FastQCFastQC Report
Sat 31 Dec 2022
SRR5847047.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5847047.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences138835
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACTTTCCGAAATCTCGTATGC5860.4220837685021788No Hit
CTTATACACATCTCCGAGCCCACGAGACTTTCCGAAATCTCGTATGCCGTCT3320.23913278352000575No Hit
ACTGGTGGGGATCTAACTGAGGGACAAAAGGAAATGAAAAAGGAACTTAAAC1960.14117477581301544No Hit
TAATTCGGGAGACAGACTGGTTGGATGGCAAGCATGTTGTGTTCGGTCACGT1790.12893002484964167No Hit
TATTCTGGGATTGAATGTAAATGAACTAAACTCTCCAATTAAAAGACAGAAT1780.1282097453812079No Hit
TGAGATGGGACGTGATAATGACTTACTGTTTTGTTTTCATTATTTCCTCCTT1750.12604890697590665No Hit
AGTTTAGGGAACCACAGCAGTGCTCAATTGTGGCGAATTACTTCTCTATCCC1730.12460834803903915No Hit
TACTTAGGGACCTTATGCCCTGTTGAATTTTTGGTGTGAGATTTTTGAGTAA1730.12460834803903915No Hit
TAATAGGGGCCCCTGAAGGATTTATTATAGCAGCTAACTTCTAACATCCATA1720.12388806857060539No Hit
CTCACAGGGAGTACTTTAGACTAATTGTGTTTGTCCGTCCATGCCATGAATG1700.12244750963373789No Hit
CAGACAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1700.12244750963373789No Hit
TGATTAGGGAGCGGCTGCAACTGCGGCTCCTGGCCAGACCCCGGCCTCAGCG1680.12100695069687038No Hit
TGGCGTGGGAGATTGGCAGTCTCTTTCGCTTATCTGCTGTTTTAAAGAATTC1670.12028667122843664No Hit
GTCTATGGGATATCAGACAAAACGGACTTTAAGACAACAGCAGTTTTAAAAA1650.11884611229156913No Hit
GCGCAGGGAATCCTATGGGCTTGCAAATAAATAACTTACTTGTTCTCTTCAG1640.11812583282313537No Hit
TTCGCCGGACGATCTATGCTGGGGAAGTAAAATGTGGAAAGAATTTAAATTA1630.11740555335470163No Hit
TAAACTGGGACCAGGGAATTCTTACTGGCAAAATGATCAATCTGGAGTGTGC1630.11740555335470163No Hit
ATAACTGGGGGAACCAAGTTGGAAAACACTCTTCAGGATATTTCCCAGGAGA1630.11740555335470163No Hit
CACTACGGGCAGTGTGAAATTGGACAAAAGTGAAAGCCACAAAACAAACTGG1610.11596499441783412No Hit
GTAGCTGGGTCACGCGGACCCAGAACCCCATGGTGACCGGGACCTCAGTCCT1600.11524471494940036No Hit
TAAAACGGGTTCTTCATAAATTGCAGTAACTTGAAAGCAGTTGGGAAATTAG1600.11524471494940036No Hit
CAATACGGGCGATCTTTCGCGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCT1600.11524471494940036No Hit
CTGCAGGGAATTGTGTGGCAGTGATTGGTCTGTTTGTGGAGAATGTATGAAA1600.11524471494940036No Hit
CTTTTTGGGGGGAGTGATTTACCCGTAACTATTTCTTAATAGCCCAGCAAGG1570.11308387654409911No Hit
TTCACGCGGGATTACTATTTAGTTTTAAGCAATTTGTAGGCATGCATTTTTT1570.11308387654409911No Hit
TAACTGCGGGCAGGTTGTGTTCATTCTTTTCCTGGAAATGATGACTTTATTG1560.11236359707566537No Hit
GTACAAGGGGACTCTTAGAAGTGCCCGAGATGGGGCTAACCTTTATTAAACA1550.1116433176072316No Hit
TTTTTAGGGATTTCACAAGTTATTTATTTATAGAATGAAAACTATGTAGTTA1550.1116433176072316No Hit
ACAAGAGGGAGTACCATAAGAGCATCACCCCCAATTTTTCTTTGAAAGATGT1550.1116433176072316No Hit
ACGATAGGGGGTGGTCCAAAGAAAACAAAATTGGGTTGAATGTTTCATTTAG1550.1116433176072316No Hit
ACTCTCGGGAGTGCATAGATCATTTAGTCCAAGAACTTAAAACTAAATAGAG1540.11092303813879786No Hit
GTTTCCGGGGAGGCAGCGAGAGTACGCGTGTGCCTCGCGCCGGTCCACGAGG1540.11092303813879786No Hit
AGCAAGGGGGGCAGAAGGGCAGAGTGTGAAGCGATTGCTCACAATGGCAATC1540.11092303813879786No Hit
ATCCTAGGGGCTTGGGAAGATGGCGGCTGTGTCGGTGTATGCTCCACCAGTT1530.1102027586703641No Hit
TCATACGGGATCTTAAAACTCGATCACCAGTCACTTTCCTGTCTGATCTGCG1530.1102027586703641No Hit
GATGTGGGATCTGAGCATGGGTATGATTCTTGAGGTAACTCTACCAGATACT1530.1102027586703641No Hit
ATTGGCGGGGAATGAGATGGAAACCAAAATAGTTGTTCCATCCTCTTACCCA1520.10948247920193035No Hit
CTCGTCGGGGTAAGGGAGGATCATGTGTATTCTTAAGTGTGGGTCCTTATTA1520.10948247920193035No Hit
CTAGCCGGGATCTAGGCAACTAAAAACAAACATTGTAAGAATGAATATTTTT1510.10876219973349659No Hit
TAATTAGGGCTGATGAGACTAGAGTGACAGTGTTTCAGAACCCAAATGTCCT1510.10876219973349659No Hit
AAAAATGGGTAGGTTTTTTAAACAGAATCCTACTGTTAATGGTAATAACTTC1510.10876219973349659No Hit
TGTTAAGGGACTTTGGGAAGTTATTTCTTTATTTTAATGATGTATCATTGTT1500.10804192026506285No Hit
CATTTGGGAGGAAGAAATGTGGTAAGACGTATACTTACATTTTCCTAAGACA1490.10732164079662908No Hit
AAGCGTGGGGGATCTCGTCAAGGGGCATAGATTCTGTGTAACTTTTTACTCT1490.10732164079662908No Hit
CAACCAGGGGGCAGGGAGGTGGGGAGATGCCCACCGATGCCTTCTGCTTGTG1490.10732164079662908No Hit
TGATGTGGGGTGGGATGTGAGAGTTGGAAGACAGCTTGCGGGCCTGCTCCGC1480.10660136132819534No Hit
CTAAAAGGGGAAACATTGTCAAAGAAAGAAGGTGTGGTGCAAAGACTTCTGC1480.10660136132819534No Hit
TTCGCTGGGGATACATTTTATCCTTTTCTCATTTGCTGGGTGGGGGAAGTGT1480.10660136132819534No Hit
CAAAATGGGAGCTGAGATTTGAATCCCTGTAGTCTTTTCTTCTTTAATTTTT1470.10588108185976158No Hit
GACTGGGGGGAATTGTTCATGAGTACTTACTAAATGAATAAGTGAAATGACA1470.10588108185976158No Hit
GTAATAGGGGGAGGAGGGACAGGTAATGTGATTCTCCCTCCTCAACCTCCAC1470.10588108185976158No Hit
TCAGTAGGGAGACATACATACAGCCAACTAGCGTGTTAAAGAAAACCTCAAT1470.10588108185976158No Hit
ACAAAAGGGATTCTGGGCTGGAGAATTCTTTGTTTTCAGGGGTTGTCTTGAA1470.10588108185976158No Hit
AACAAAGGGTTCCTGCCAAAAGCATCACAAGTTGTACATCATCAAGGCTCCC1450.10444052292289409No Hit
ATAGCAGGGCACCCAGTGGCTGGCGCTGTGGCTGGCTCCATAGATGTCGCAC1450.10444052292289409No Hit
GCATTAGGGGGTCCCCAAACAACCACTTAGAGGCTAGCTGGATATCCTGCTG1450.10444052292289409No Hit
AGACTAGGGTCTCCCTAGTGCGGGCTGGCAGTGCGGGCAGAGCCCGGCTGAG1450.10444052292289409No Hit
AAGAATCGGGTAGTTACTTTGAATATACCATCTTCATTTTTGTAGCTTTTTA1440.10372024345446033No Hit
ATTGCTATTGGGGAGGTAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTG1440.10372024345446033No Hit
CTGCCTGGGACCAGCTGTGGTGGCTAAAGGGAATATGTTAATTAAGCAAGAG1440.10372024345446033No Hit
GGCTAGGGACGACAGCTTTGATAAGAATGTTCTGGACAGTGAAGATGTTTGG1440.10372024345446033No Hit
AACACTGGGCTGTGACAACAAGTATAACTCAAACCATTGTTTGTAACTAAGA1430.1029999639860266No Hit
GCAACTGGGATATGAAAATAAAACTGCTTTAAAAAGTGACCTGAACAACATA1430.1029999639860266No Hit
TTGCTTGGGGTAAGCACTTTAGGCTTCATAGGCCAATAGTCAAAAAAAATCA1430.1029999639860266No Hit
ACGTGGGAATTATCCAGGATTTTAAATCCTCAACTTGTTCTAGCTTGTGATC1420.10227968451759283No Hit
GATTGAGGGAGAACGTGGGTGACTTTTTCAAAGTTTATAATTTAAAAAGCTC1420.10227968451759283No Hit
AAAAACGGGGATTCACTAACTTTCTGTGCTAGATATAGTTCATGCTTAGCAT1420.10227968451759283No Hit
CATCCAGGGCAATCATGAAGATACATAATTACAAACTGAGACAAGTGTCATA1420.10227968451759283No Hit
CTGAGGGACGAGGATGAAGTAGAGGATATGGAAGAAAATTAAGTGTGATCCA1420.10227968451759283No Hit
GCGCAGGGGAACTTTGAAGTCAGGATAGAATATCATTAGATTATCTGTGAGA1410.10155940504915908No Hit
AACATAGGGATAGGATGGGAGATATTGTTGAGGACATCTTTGGAAATTAAAT1410.10155940504915908No Hit
CTCAGATGGGGTAGATAGTTTTGTAGAAGACTAGAAATCAGTTTCTAAATGT1410.10155940504915908No Hit
AAGCAAGGGATCTCATGAAAAATGAATCTCATGGAAATATTGCCTTATTTAT1410.10155940504915908No Hit
TGTGCCTGGGCAAAAACAAAAGAAGCCTTCCTCTCACCTCATTTTGTAAATG1400.10083912558072532No Hit
TAAGTGGGGTTAATTGAGAGGTGAGAACATAAGATTCCTCTGGAAATTTGCT1390.10011884611229158No Hit
CTTCATGGGCTCAGGAAAAAAAGGAAGATTGAACTAATAAAATTTTATTTCT1390.10011884611229158No Hit
CTTGGCATGGGGCTCTTGTGCTGGTCTGTTCATGATATACTTTCAGGTAAGT1390.10011884611229158No Hit
TGCTTTGGGGCTGTCTGGTGCTGCTGTTATTGACTACAGTGTTGTACAGAAT1390.10011884611229158No Hit
ATTGCAGGGAGAGGTGGAGTATGAACCCAGGTGTATCTCTAAAGACCATGAC1390.10011884611229158No Hit
AAACAGGGGGCTTTCCTTCTTGTTTGAATATATTGGTGAAATGGGAAAAGAC1390.10011884611229158No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)