FastQCFastQC Report
Sat 31 Dec 2022
SRR5847034.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5847034.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences146100
Sequences flagged as poor quality0
Sequence length52
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACTTTCCGAAATCTCGTATGC9310.6372347707049966No Hit
CTTATACACATCTCCGAGCCCACGAGACTTTCCGAAATCTCGTATGCCGTCT8320.5694729637234771No Hit
TAAATAGGGGCAGCAATTCCTTATTCTTTAATCATATCCTTAGGGTATTTAG1880.12867898699520874No Hit
ATGCTGGGATGACTCCTTCAAGGAAAAGAGGCCCTGCTCCCTTTACCTGCTG1820.1245722108145106No Hit
CGATTTGGGGTTGGCCAGGCTGATCTTAAACTCCTGCAGGAAATCCACCTGC1780.1218343600273785No Hit
TCTCAAGGGGAAAACTAAGGAGAGTCGTGAAGCAGTTGAGAAAGAATTTGAG1760.12046543463381246No Hit
TTGTCGGGGGAGCTGCCAGAATCCTCTCTAAGGCTTTCATAAATTATTAAGA1730.11841204654346338No Hit
AGTAGGGGGGGCAGAAGGGAAACGTGAAGAAGGTGAAGATGGCGGTGGCCAG1730.11841204654346338No Hit
TCATCAGGATCCTATGTGAGAGAAAAATTGTATTCATTACGAACAAAAAACC1710.11704312114989733No Hit
AGGCTTCTCAATCAGGAAAAATCAGAACTGCTTGTTGAACAGGGTCGTCTAC1690.1156741957563313No Hit
TTGTTTGGGCTGGGCGCGGGAGGGGTGATCTGGGCATCTGTTGCAGCAGAAG1680.11498973305954827No Hit
GCTGAGGGGATGTGGATTTTTGAAAATAAATATATTGGAAATATTTTTTGAG1680.11498973305954827No Hit
TTGCTTTGGGAGGGGCCAGGATCCAGTTCCCTCATCTCTGGCAGGAAAGATC1670.11430527036276524No Hit
TAGCTAGGGAGCTAAGTGATTATGTCAGTTTATTTAATTTTGTCTAAGAACT1670.11430527036276524No Hit
CCTCTTGGGAAGCCTTCCACTGTTAGAGATTTGAGGTTACATGATATGCTTT1670.11430527036276524No Hit
AAAACTGGGGAAAAGACTTCTTGTGCAAACTATAGACAGGTGACCTTGCTGT1660.1136208076659822No Hit
TGTAGCGGGCTGCATCAGGGATGGCACATGCAGCGCAAGTAGGTCTACAAGA1650.11293634496919917No Hit
TAGTTGGGGACGTGAAAAAATGCTCATTATCTCTGGCCGTCAGAGAAATGCA1650.11293634496919917No Hit
TACAAAGGGGGGGAAAAATCCTAAAGCTGTCTTCCAAAAGGAAAGGTAAAAT1640.11225188227241614No Hit
TCGAATGGGCTCCATCTACATGAAAGAAAAGTTACCTAACAAGAAATTTGAG1630.11156741957563314No Hit
TTGCGCGGGGGTACATGTATCTGCATAACTCATTTTCAAAAACATCTCTAGA1600.10951403148528405No Hit
TATCATAGGGACAAGAAGCAGAATTCTTTCAGCTCAAGGGACTGGCAGAGGA1590.10882956878850102No Hit
AACTGTGGGATTTATAAGGAGGGTTATTCTTTTTTGTTTTTTGTAGGTCTGT1580.10814510609171799No Hit
CACAGTGGGGTCCGATCTATAGGAGGGCTATGAAAGTTTCTGTATAACAGGC1580.10814510609171799No Hit
GCCGTAGGGAATGAGGGATCTGAAGGAAACGGCCCCAGAGTCACCCATCCCC1580.10814510609171799No Hit
ATACAGGGGAGTGAGTTCTGATGTGTGCTTTTTGCAAATACATGTTCTCAGA1570.10746064339493498No Hit
CACTTAGGGATTGCAAAAGTCATCATGTGTGACAAAACATTTTCCACACTGG1570.10746064339493498No Hit
CGTAATGGGGTACCTGTGCCTAGGATTGGTTTTAAATTTGTAAATAATTTTC1560.10677618069815195No Hit
TGCAAGGGGATAATCAGATGTGTTATAAGCCAAGTGCTAACACATAGAAGAC1560.10677618069815195No Hit
AGTTTGGGCGATCTATTCGGGGGATGCTTATGTGGTTGTTCAGTTTGTTCCC1550.10609171800136892No Hit
GACAATGATAATTTTGTGTTGGATCCCTATTCTGGAGTCATAAAGTCAAATG1550.10609171800136892No Hit
ATTGTCGGGGGTTCATGATGACACAGGACCTTGTCTGAACATAATGATTTCA1540.1054072553045859No Hit
TGCCTGGGGGAGGTGCTGCTTTCCTTTATTCTCCTCTGTGTAGAGCTTTTCC1540.1054072553045859No Hit
TTAACAGGGATTCTGAATAATTAATAAATTGGGCTGTTTTCTTTTGGCAGGA1530.10472279260780287No Hit
TGTTTTGGGAACATGTGTTGGGTAACTGATTCTTGCACTAAGTTTTTAGAGG1530.10472279260780287No Hit
AGTGCGGGGAACTGACGGCAACATGTAAACACTCGAAAAGCAATGACACTAT1530.10472279260780287No Hit
TCAAGTGGGCTGGAGTGGAATACAATAATCTAGTTGTATGCAATGATGCAAT1530.10472279260780287No Hit
ATTCAAGGGCATGGGTTCAAACCAAATCAGCAGGTCAACTGAAATTAGAACT1520.10403832991101984No Hit
GTACTAGGGATAAAACTTAAAACTTTACAGTCAGAGGTTCAATTCCTCTTCT1520.10403832991101984No Hit
TTTCATGGGAGTTACATTGGTTTTAGATTGTGGTTTCCACATCACTGTAATA1520.10403832991101984No Hit
ATTGTAGGGACCAGTCATATGATGACAAAAAAATAATAATTTGAAACTATTT1520.10403832991101984No Hit
AACTAGGGGCCGATCTGGCCAGGGATATTAGCATGGCTATATTTGCTAGTAG1510.10335386721423684No Hit
CTACAGGGAAGATTATTTCATTTTAAATGCATAGGCTTCTCAAATTATATTT1510.10335386721423684No Hit
TTTCTTGGGACTGATTTATCACTCTGAGTTCAAGATTCATCTTCAGAATCCT1510.10335386721423684No Hit
CCAGATTGAAGAGACAGGCAAATACAGAGAACAGGAGCAGAAGCCTCTGCAG1510.10335386721423684No Hit
CGTAATGGGAGACAGGGTTACCTGGTGAGGTGCAGAGAGTCCCTCTAGTGGC1510.10335386721423684No Hit
CAGTATGCTTTAGCTGATAAACAAAACAAAACAAAAATTCCTCTTCTTCCTG1510.10335386721423684No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTTTCCGAAATCTGTCTCTT1510.10335386721423684No Hit
GTATCAGGGAGCCAGAGAAGAGGGTCAAAAGAACGATAAAATAGAAAACAAC1500.10266940451745381No Hit
TCTCTGGGGGAACTCATTTCAGGTTTGTAATATCCAATTAAGATTTTAGTTT1490.10198494182067078No Hit
GATTGTGGGGTTGGAATAGGATGTAAATAAGTGGAGGTGGGCATTGAAAGTT1490.10198494182067078No Hit
TGTATCGGGAAGAGTCTTCATGTTTGATATTTAACATCCCAGAGCCTCAGTT1480.10130047912388775No Hit
GCTCAGGGGATGTCATGGTTGCTGGTTGCCCTTAACTGAATGATTTTGAAGG1480.10130047912388775No Hit
GAAGCAGGGAGGGGCAGGGAAGGGGGTGGTGGAGCCCAGTGAGAGTTGGAGG1470.10061601642710472No Hit
GTAGGCGGGAACATGCTCTAATTATAAATCACTGCATTAAGGACAATGAAAA1470.10061601642710472No Hit
TTTATAGGGATGAACCACCTGTGCTCGTGCCCGGGTAGCTCTGACTGGGCTG1470.10061601642710472No Hit
ACTTTAGGGGTTAGATGAAAGGATATGAGAAAGGTAATATTGGTAAATAAAG1470.10061601642710472No Hit

[WARN]Adapter Content

Adapter graph