FastQCFastQC Report
Sat 31 Dec 2022
SRR5847028.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5847028.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46760
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACTTTCCGAAATCTCGTATGC5051.0799828913601368No Hit
CTTATACACATCTCCGAGCCCACGAGACTTTCCGAAATCTCGTATGCCGTCT4620.9880239520958085No Hit
ATTAACGGGAGAGAATGAGAGACATGTGGAGTAGACCTGATCTGACCTCTGT2080.44482463644140285No Hit
GTAACAGGGTAAAGAGGCAATATCTAGTAGCTAAATAATAGCTAAAATTGTT2000.42771599657827203No Hit
CACGCCGGGATCCCTGCTACAGGCCCCAGTGTGTGTTGTTCCCCTCCCTTTG1270.27159965782720275No Hit
TTGCATGGGACAGACCAACTAGGCTACATCTGTGATGAGCAGCTAGCAAAGC1050.2245508982035928No Hit
TTAGCAGGGTGAGATTTTTAACATGAAGGGATGTTGAATTTTATGGAAATCC1040.22241231822070143No Hit
TTAATGGGGAGCTTAAAACATTTTCTAATTTTCATTTTATTTCTCTTTTGAC1010.2159965782720274No Hit
AAGTGGGGGGAAATTCTGAAGTTTAGACCAAGTTGCCCATTTCTGCGTAATT1010.2159965782720274No Hit
ATTCCAGGGTTTGTCTAAGCACTGGCCAGTCTGTTGTGGGCATTGTTTTCTA1000.21385799828913601No Hit
AAATACGGGCGCTGATCTGGATGTGGGGTGCCAAAGTACCCTTGTGATTCAG980.20958083832335328No Hit
GTTAGTGGGAAGGAGCTGGTGGAGATTGCGGTGCCTGAGAACCTGGTGGGAG950.20316509837467922No Hit
TACTCAGGGGAAGCTGTGCCTGAAGGGTGAGTGTGGAGCTTGCCCCTCCGGC940.20102651839178787No Hit
TTACCAGGGGGTAAAAACAACTCAGAATACAAAATCTTTATAAAGGGAGGCC930.1988879384088965No Hit
GAAGCAGGGTGGAGGATAATGAAAAGTCCGTGTTTTGAAGTTTTCAGATTAT920.19674935842600513No Hit
TGCTATGGGTACATCTGCAATATTGTGTTTTTAACGCCAGCACCCAGAACTT900.1924721984602224No Hit
CTGTCAGGGACTGGAGTTTTTGGTGCTGAATAGAGCCAGTTGCTAAAAAATT880.1881950384944397No Hit
TTTGCAGGGACCTAAGAACAGCTAAAAGAGCACACCCGTCTATGTAGCAAAA870.18605645851154834No Hit
TGTCAAGGGCGGCGTTTGGGCTACGGTTGTTGTTGGCGACTGTGCTTCAAGC870.18605645851154834No Hit
CTAAATGGGATGGCCCTAAGCATGGAGAGTCAATATGTTTAACAAGCTAAAC870.18605645851154834No Hit
GTATCTGGGAGTGAAGCTGATGCCTAGGAACTTTTAAAGGGATCCTTTCAAA860.18391787852865696No Hit
CAAAACGGGGAATAATTAAAGATAAAGCACTTCTTCCCAAAGGGAATTATCA860.18391787852865696No Hit
TGTGCTGGGTAGTGAGGTGTGTGTATTTGTGTGTACACACATGAGATGGACA850.1817792985457656No Hit
ATAGCAGGGAGCACTTTTTCCTTCTGAAAATATTTACATTTTGCTAACCTAG850.1817792985457656No Hit
CACTCAGGGAGCCACAGAACTAATCATATTTTATATCTTCTTCGAAACCACA840.17964071856287425No Hit
TCATTAGGGATGAAAGAAATCTTAGATAAGAAGGTTGAGAAGGTGACAATCT840.17964071856287425No Hit
CATTCTGGGGTATTGCAATGGTATGAATCTGCTCATGTGGAGACTGGCTATA830.17750213857998287No Hit
GTTCTTGGGCTGGTTCCTGGAGGCATCAAAGGCTGCATCCGTTCTGCCAACA820.17536355859709155No Hit
AGAGTAGGGAGTTGCAGCTGATGCCTGAATGTATCCTAGCTGACAAATTATT820.17536355859709155No Hit
GAATTTGGGTCGTGGCAGAGTTGACTGGAGACAACATTCCTAAGGACTTTAG820.17536355859709155No Hit
CTGTAGGGATGGGAAAAGATTTGATAACACCAATCAACAGGAAGCTGGGGTG810.17322497861420016No Hit
ATGATAGGGAAGTATACCAAACAGAAGTAAACATATTCACAAGAAGACAATC810.17322497861420016No Hit
ACCGCAGGGATGTGTGAAAAGGACGCAGACTGAAAACGTTTTGGTTTTGTAC800.1710863986313088No Hit
TGTAAAGGGATCTCATGGTGTGCGATGAGCAAACGCCTGATTATTCATTATA790.16894781864841746No Hit
ACCTCACAGGCCCACCCCCTGCCGGGCATGCCGTGGGATCATGGGCAGGGAA790.16894781864841746No Hit
TAATCGGGTGATCCAGCAGTTCTATGCCTATATACATGCCCAAAGTAATTGC790.16894781864841746No Hit
GAACTTGGGCCAACAGGGAAATGGCAAATAGCCCAGTGTGCGTAGAGTTTAC780.16680923866552608No Hit
ACCATAGGGGACCCAGAAGGATGCCACCAGTTTTTCCTTGAGGCCTAGGATT760.16253207869974337No Hit
TGTCTGGGGATTGCATATTTTATGTATTTGGACCAAAAGGTTACAAGTAATT760.16253207869974337No Hit
AGCCTGGGTTATTTGGAACTGGAAACATGTTTCCCCACGAAAGTTTTGTTAT760.16253207869974337No Hit
GTATATGGGATTCCACTTTAGGGAAATTCTCCTTTAAATGGGTCAGAAAATA760.16253207869974337No Hit
CCATCAAGGGTTCTTGATGGTGCCCTTTGAATAAGAAAAGTTTTACATTTTC750.16039349871685202No Hit
TATTATGGGCATCACAAATCCAAGAATGTTTGCATCCTAAATGCTAGTTTGC740.15825491873396064No Hit
CGTAAAGGGATGAGAACAAGAATAGAGTCATCATGTATTCTTTGTTATTTGC740.15825491873396064No Hit
TGCAATGGGACCCACCATATTCTTCCTTGAGGAATTACAGCATGTAATTCTA730.1561163387510693No Hit
TAATTAGGGATTTTTTAGACAGTTTGATTATTCTTAAAAATTGAAAACATAA730.1561163387510693No Hit
GGTTTAGGGATATCCAAATTCAACTGTTTGGTTCACTCTTGGTGCCTCATAT730.1561163387510693No Hit
TGACATGGGAGGTGATTAAAAGACCTTGAAATCCATGACGCAGGGAGAATTG730.1561163387510693No Hit
TGAAAAGGGATGTAAAGATTGTATTACATTTGTATACAAAATTTTTTTGCAT730.1561163387510693No Hit
CTCTGTCGGGGATATACGGTATCAGAGACTGGTGGTGGAGCTGCTTATCAAA730.1561163387510693No Hit
TAACCAGGGTCCATGGTTGATGTTAAAGTGGATAAGATAAAAGGAGGAAAAT720.15397775876817793No Hit
TACATGGGAGTCATGAACCACCACACCTGGCCTACATGATTTTTTTAAATCC720.15397775876817793No Hit
TAAGCAGGGGAAATTCACCATGAAAAAACAAGAATAACAAATAGAAGGGAAG710.15183917878528658No Hit
GTACCAGGGGCAACGTCTTAAAAACAGGCGTGGTGCGCTGCTGCGTGGGACA710.15183917878528658No Hit
TCAACAGGGAAAGGGCTAGATTCTGGTAAATGGCATATATGAATGCACAGTT710.15183917878528658No Hit
CTTGTAGGGATAGACAAGTGGGAAAAATGGTGTTAATACTTTCTAATATTTC710.15183917878528658No Hit
CATGGTGGGCTGCAGAATTCCTCAGTGTTTGTTTATATGAAAGTTCATTGTG710.15183917878528658No Hit
GCTGTAGGGGTGTTCATTTTTTACTACATTTTATTAAAACCTGCTTTATACT710.15183917878528658No Hit
GGGCATGGGTATTTACTCAAAACATATGCCAAATTTAGTGTTGAAAGGCTCA700.14970059880239522No Hit
GGCTATGGGTACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACT700.14970059880239522No Hit
GTTTAGGGGAGAGCACAGCTTGGTTTTATACATTTTAGGGAGACATGAGACA690.14756201881950384No Hit
TAATATGGGGCAATAGGCTATAAGGAATAGCAGGAGAAATTTTACTGAAGTG690.14756201881950384No Hit
CGACATGGTATTCAGACTTCCGATGGGCTTATCTTGTCCCCTCCAGGTACAA690.14756201881950384No Hit
TAGATAGGGAAGACGAGAAGACCCTATGGAGCTTTAATTTATTAATGCAAAC690.14756201881950384No Hit
AAATTAGGGAATATATATGTACCTAATATTGGAACACCAAAATATGTAAATC690.14756201881950384No Hit
CATGCAGGGGACTATACAAATTGTAAAACAGATACTATAATATTTCCTTTAT690.14756201881950384No Hit
GAGCTAGGGAATGCCCCAACTAAATACTACCGTATGGCCCACCATAATTACC680.1454234388366125No Hit
TGCTTTTGGGAAATTATTTCAACTAGAAAGCAAAAATAGGTGGCTTCGACAG680.1454234388366125No Hit
TGTCTTGGGGCTTCTGGTACCTGATTTCCTGACATTGCTATTTATATGATGC680.1454234388366125No Hit
TTTCAGGGGGCTCTTTTTCCGGCTGGAACCATGGAGGGTGTAGAAGAGAAGA680.1454234388366125No Hit
GTACTTGGGTACCAACAAGAAGGGAATGCAGGTAGTTGTTTAGGAGATGGTA670.14328485885372114No Hit
TGGTGCGGGGGTGGCAGGGCCAAGGTCCCCACTTTCTTGCTGACTGTAAACT670.14328485885372114No Hit
ACTCATGGGAAATGGGTTACCTCCAAATATGAAAATCTATAACAACCTATGG670.14328485885372114No Hit
ATGCATGGGATTGATGTGTTCCATGTCCAATTCATGAAAATAAACTGTTCTA670.14328485885372114No Hit
TATCGGGATCTTGATCTGTTTCCTCTATCTTGTTTCAGTGCACACGCTTGTG670.14328485885372114No Hit
CTTATACACATCTCCGAGCCCACGAGACTTTCCGAAATCTGTCTCTTATACA660.14114627887082978No Hit
GACCTAGGGGTGGATGGCCCCTCCGGGAAACTGTGGCGTGATGGCCGCGGGG660.14114627887082978No Hit
AAAAGCGGGAGCCATCCCCTCCTCAGCAAGGCATCTACCGGCCCCTCACAGA660.14114627887082978No Hit
AATAGAGGGATATATAAACACTCCACAGTGTTTATATTGGGAAGATATTGGG650.1390076988879384No Hit
GTTCGCGGGTCCTTTTAAGAATATGGAGATGAAGATTGTTTTCTCCAATTTT650.1390076988879384No Hit
ATGTATGGGGGGCAGAGGTGCACTTTATGAAACTGGCACTTGACTGAACAGA640.13686911890504705No Hit
CACAGCGGATTTTGTGCAGTGATTATTTTTTAAAGTCTTCTTTCATGTAAGT640.13686911890504705No Hit
ATACAGGGGGTGCCCTTGGAGGCAACCTCCCATTTTAGATGTTTAAAGGACC640.13686911890504705No Hit
GTTTAGGGATGATGAAACTTCGGCTCACTCCTTGGCGCCTGCCTGATCCTCC640.13686911890504705No Hit
CAACATGGGAGTGCTCTGAGCCCTAGGATTCATCTTTCTTTTCACCGTAGGT640.13686911890504705No Hit
GTAGCAGGGATCTGATATCAGGAAATATCTGTGAAGGACTTGGTGATTACCT640.13686911890504705No Hit
ATCGCCGGGTATATATTGTTGTTATCCTCATTAGATTAATAAGGAAACTGAG630.1347305389221557No Hit
GAATTAGGGGGTTTGCTGTGGAGGAAATAATGTACTAGATCATTGTCACAGA630.1347305389221557No Hit
TACCCAGGGCAGATTACTCAGTGCTATAATTTTCTTTTAGTTAAACAAAGGG620.13259195893926431No Hit
GCTCAAGGGGAATGGTGTTTACAGTGCTGCAAATTACACCAATGGGAGCTTT620.13259195893926431No Hit
TTTCGGGGGCAACTTCAGGCTGATCAGCAGATGGGATGTGAAAAATACTACC620.13259195893926431No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTTCCGAA620.13259195893926431No Hit
AGTGTTGGGGGTTTGATTAGATTTTTTAAAAAAATATGACTTCATCTGTGAT620.13259195893926431No Hit
GGATATGGGAGTTTGATTTTTCTTTCCATATTTGAATTAATTTTTTCTGTTT620.13259195893926431No Hit
TTATGAGGGCATTCTGGATTATTGTTCCAGCATTGCACAGTCCCACTAAACC610.13045337895637296No Hit
TTCAATGGGAAGACTGAAGGTTTTTTTCCCTTGACATTATAATCTACCAGAA610.13045337895637296No Hit
ACTGTTGGGGAAACCGGTTAAGGGAGAAATTTTAAACATGGCAAACTTAAGA610.13045337895637296No Hit
ATCAAAGGGTGTTCTGTGTCAGTTTCCTCTGCGCCACGTGCTTTCACAGTTT610.13045337895637296No Hit
CCAGCGGATTAACATTGGAAAATAGACATAATCTTTAATTTTTAAAATATTT610.13045337895637296No Hit
TGACGGGGACAGCAAGACGAGAAGACCCTATGGAGCTTTAATTTATTAATGC600.1283147989734816No Hit
AGTTAAGGGCCTGGAGACCGCGGCCAAGCACGAGCTGGAGCTGATGGAACTC600.1283147989734816No Hit
TACTATGGGATAGGAGATTCTGCTTATTGATCGGCTTGTTGCCTGTCTGCTC600.1283147989734816No Hit
GGGTAACGGGCAGTATACAAGAGTCAATTGATTTCCTCTAGACCAGCAATGA600.1283147989734816No Hit
GTCACCGGGTAACAGCCCCAAGACCAGCCAGAGGGGCTCTGATGATCAGCCC600.1283147989734816No Hit
GACTTGGGACATATTTCAGTATAGTAAAAAGAGGAAGTCTATCACTGTAGTG600.1283147989734816No Hit
CATACACGGGACACGACGCTCTTTCGATCTAATAACGGGAACATTAAGCATG600.1283147989734816No Hit
CACGCTGGGCTGTATCTAAAGAATAGATTAAAACATAGGTTACTATTTATTA600.1283147989734816No Hit
GTGCTAGGGCTAATTAACTGTTGATGAGCATTTTGGATATTCTAGGAGAAAG590.12617621899059026No Hit
TGCAATGGGGAAGGTCATAAATCTTTCAGAGTGACATCACCAACTGTACTGC590.12617621899059026No Hit
GCTCCAGGGAACCTTCCCTCGGAAGGAGACAATTCGAGGTGCTGGTACATTT590.12617621899059026No Hit
GTCTGGGGGCAGGCCCCACTGACATTAAAAGAATACTAAAGGAATGTCACAA590.12617621899059026No Hit
AATATAGGGATTTCAGTTAAAGTTTGATTGACATATTTTTTTCTCAGTCTTT580.12403763900769887No Hit
CCAACAACGTGAAGACGGCCGTGTGCGACATCCCGCCCCGCGGCCTGAAGAT580.12403763900769887No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTTTCCGAAATCTGTCTCTT580.12403763900769887No Hit
CAGCTCGGGAATAATAAACAATGTAGTTCCCTAGCATCATTTGATTAAAAGG570.12189905902480754No Hit
AGTTAAGGGCACGACGCTCTTCCACTCTACGGCTGGGATTGCCATGGTTGAG570.12189905902480754No Hit
GGATCTGGGTAAACAGTTGTTACAATGTATTGTCAAAGACAAGCTCTCCTTG570.12189905902480754No Hit
GGGACTGGGATGCATGTGTCTCTGTGTATATGGCATATACCTAGTAAGTATG570.12189905902480754No Hit
AGCATAGGGATTTTGGAAGCAGCTGCTGGTGGCTTGGGACATCAGTGGGGCC560.11976047904191617No Hit
AACCAAGGGATAAACAATGTTAAATAAAGGTAGACTCAGTCTTTAAGATATT560.11976047904191617No Hit
ACACTAGGGATTACTGAGTAGTGTCAATGAGGGTGTGGCAAAATGGAGCCTT560.11976047904191617No Hit
CTAGAAGCGGGTCTGACTAAGGGATTCTGTTCTGGGGTCATTTTTGCAGGCC550.1176218990590248No Hit
TAGTTAGGGCAGAAATAAAATGTTTATGCTATTGTTCACTACTGAAATGATG550.1176218990590248No Hit
ACTCACGGGGAGACAAAGGGCCTTATTCATAAGTGGACAGGTTTCCCCATAG550.1176218990590248No Hit
ATGTAAGGGGGGTACACACTGAGGCCTTGATGATAACATTGGAAATGATGTG550.1176218990590248No Hit
CTCTATGCGGGCAACAAAATATGTTTTTACTGGATAAAAACAAGTTTTGTTC540.11548331907613345No Hit
CCTAACGGGGGGGGGTGGCGCTGGGGACAACCAGAAAGTTTCAAGCGACAGG540.11548331907613345No Hit
TCCTGAGGGCATACCGTTTACTAGCAAATAAAATTTTCTGATCTGTAAAAAC540.11548331907613345No Hit
CATCTTGGGAAGTGTACAGTATTTACCTTCCTTTGTCTTACATTGGCTTTTT540.11548331907613345No Hit
ACAAGGAGGTGGGTGGATATGGATTCTAAGGATTCTAGCCTGTCTGAACCAA530.11334473909324208No Hit
GTTAGGGAAGCACTAATATTCAGGCCTTACATTATACCAATTAAATCAGAAT530.11334473909324208No Hit
TAGCTTGGGAACTTTGAGCTGAGTGAGGGGAGAGGGGTAAAGAGAAAGAAAC530.11334473909324208No Hit
TGTCAGGGGCAGTGGAAGTTGTAACTATCTGCCACACTATATAACAGGAGCA530.11334473909324208No Hit
GCATTCGGGCACCATGGCTAGTTTTTCCTCTGAAGATTGTTCGCCTCAAGGC530.11334473909324208No Hit
TTCTATGGGTACTAAGCATATGTCTTGTCTGTGGGTTTCTGGAAACCAATGT520.11120615911035071No Hit
CAAGATGGGTTAATGGAGGATGTCATCTTTTCATAGATGCTGGAACTAGAGT520.11120615911035071No Hit
CTAGCCGGGATGTGACAATTGTCAAAAAATGCACTAAAATGTAAATGGAGAT510.10906757912745937No Hit
TCTGCTGGGATAAAGCATCATGTAAAGAAATCCTAACATTTAAGTGTAATTC510.10906757912745937No Hit
ATTCAAGGGGCATAACCAGATGGCTTAAAATTTTTGTACTGATGTTTGTAGT510.10906757912745937No Hit
AAGTATGGGGTGTATTCTGTGGATTCTATATTTCATATTGAGATCAGCATTC510.10906757912745937No Hit
TAAGCTGGGGGCCACCATTGCTTCCAGTGAGAACCTCTCTATCCAGGAAAGA500.10692899914456801No Hit
AATGCAGGGAATGATATGAAATGATGAAATGATAAAAAGTGAAATGATTAAA500.10692899914456801No Hit
GGTAACGGGACCTGTATTTTGAGGACAAGAAAGCTGAATGAGGGCAGAGGGG500.10692899914456801No Hit
TAGCAGGGGGTTTCCTTTTTGAGTAGTTCTTACTATTTTGCAAGGATTCATA500.10692899914456801No Hit
GTCTGAGGGTGACATATATTGAACCTGAGCTTTGTGTTATGTTTTTCAGTAC490.10479041916167664No Hit
GCTGATGGGTGGCTTTTATAGTGACAGGCAAAGAGGAATTTATTGAGATCAC490.10479041916167664No Hit
GGCTGCGGGTGCAAAATGTTCTTCAGAATAAAACTGTGTAATAATTTTTATA490.10479041916167664No Hit
GTATTGGGGGATGCTGAGTTGGATGCCTACATGGCGCAGACAGATCCCGAAA490.10479041916167664No Hit
CACAAAGGGTCCGATCTGCTATGGGGATTATTCAAGCTATGTAAAATATGCA480.1026518391787853No Hit
ATTCTCGGGAATGAGATGAAAGAAAGCCTCGGTGTCAGGCATGTGGTAATTT480.1026518391787853No Hit
AACGATGGGATCTGTAGAGGTTTGTTGTTGTTGTTGTTGTTTTTGAGATGAA480.1026518391787853No Hit
TATCATGGGTCTATGGCCGGGGATCTAATGTGGGGATTATGAAATGTTTTAA480.1026518391787853No Hit
TTGTAGGGGAATTATGTCCATTTTGTTATAGACTAAATCAGGGGTTTGTTCT480.1026518391787853No Hit
CTGCACGGGGAGACAGAAGGACCATATTCACATAACTTTTATTACAATATAA480.1026518391787853No Hit
ATTAAGGGGCAGGCACCGCCTCCAGGACAACGTGCGCGAGGACCTCTATGAG480.1026518391787853No Hit
GAATAGGGGCGATCTCAATGCGGGCTTCACTGATGCTTGTCTGGTAATAATC470.10051325919589393No Hit
GGAAACGGGATGAGACATCTATGAGAAACTTTTGGAGCAGTGCACTCACTCA470.10051325919589393No Hit
CTCACCGGGCGCTCTTCCGATCTTCGATTGGGCCGATCTTGGTTAGGGATTT470.10051325919589393No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)