FastQCFastQC Report
Sat 31 Dec 2022
SRR5847027.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5847027.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46398
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACTTTCCGAAATCTCGTATGC4641.000043105306263No Hit
CTTATACACATCTCCGAGCCCACGAGACTTTCCGAAATCTCGTATGCCGTCT4420.9526272684167422No Hit
ATTAACGGGAGAGAATGAGAGACATGTGGAGTAGACCTGATCTGACCTCTGT2320.5000215526531315No Hit
GTAACAGGGTAAAGAGGCAATATCTAGTAGCTAAATAATAGCTAAAATTGTT1890.4073451441872495No Hit
CACGCCGGGATCCCTGCTACAGGCCCCAGTGTGTGTTGTTCCCCTCCCTTTG1580.3405319194792879No Hit
TTAATGGGGAGCTTAAAACATTTTCTAATTTTCATTTTATTTCTCTTTTGAC1210.26078710289236606No Hit
TTGCATGGGACAGACCAACTAGGCTACATCTGTGATGAGCAGCTAGCAAAGC1060.2284581231949653No Hit
AAGTGGGGGGAAATTCTGAAGTTTAGACCAAGTTGCCCATTTCTGCGTAATT1050.22630285788180524No Hit
GTTAGTGGGAAGGAGCTGGTGGAGATTGCGGTGCCTGAGAACCTGGTGGGAG1010.21768179662916506No Hit
TGTCAAGGGCGGCGTTTGGGCTACGGTTGTTGTTGGCGACTGTGCTTCAAGC990.21337126600284498No Hit
TACTCAGGGGAAGCTGTGCCTGAAGGGTGAGTGTGGAGCTTGCCCCTCCGGC980.2112160006896849No Hit
GAATTTGGGTCGTGGCAGAGTTGACTGGAGACAACATTCCTAAGGACTTTAG940.20259493943704468No Hit
TCATTAGGGATGAAAGAAATCTTAGATAAGAAGGTTGAGAAGGTGACAATCT930.20043967412388466No Hit
GAACTTGGGCCAACAGGGAAATGGCAAATAGCCCAGTGTGCGTAGAGTTTAC920.1982844088107246No Hit
TTAGCAGGGTGAGATTTTTAACATGAAGGGATGTTGAATTTTATGGAAATCC920.1982844088107246No Hit
GTTCTTGGGCTGGTTCCTGGAGGCATCAAAGGCTGCATCCGTTCTGCCAACA910.19612914349756452No Hit
CTAAATGGGATGGCCCTAAGCATGGAGAGTCAATATGTTTAACAAGCTAAAC890.19181861287124444No Hit
CCATCAAGGGTTCTTGATGGTGCCCTTTGAATAAGAAAAGTTTTACATTTTC880.1896633475580844No Hit
TGTCTGGGGATTGCATATTTTATGTATTTGGACCAAAAGGTTACAAGTAATT880.1896633475580844No Hit
AGCCTGGGTTATTTGGAACTGGAAACATGTTTCCCCACGAAAGTTTTGTTAT880.1896633475580844No Hit
GTAGCAGGGATCTGATATCAGGAAATATCTGTGAAGGACTTGGTGATTACCT880.1896633475580844No Hit
CACTCAGGGAGCCACAGAACTAATCATATTTTATATCTTCTTCGAAACCACA870.18750808224492435No Hit
CTGTCAGGGACTGGAGTTTTTGGTGCTGAATAGAGCCAGTTGCTAAAAAATT870.18750808224492435No Hit
TGTGCTGGGTAGTGAGGTGTGTGTATTTGTGTGTACACACATGAGATGGACA860.18535281693176428No Hit
GAAGCAGGGTGGAGGATAATGAAAAGTCCGTGTTTTGAAGTTTTCAGATTAT860.18535281693176428No Hit
TTACCAGGGGGTAAAAACAACTCAGAATACAAAATCTTTATAAAGGGAGGCC860.18535281693176428No Hit
TGACATGGGAGGTGATTAAAAGACCTTGAAATCCATGACGCAGGGAGAATTG840.1810422863054442No Hit
CAAAACGGGGAATAATTAAAGATAAAGCACTTCTTCCCAAAGGGAATTATCA830.17888702099228415No Hit
ATAGCAGGGAGCACTTTTTCCTTCTGAAAATATTTACATTTTGCTAACCTAG830.17888702099228415No Hit
ATTCCAGGGTTTGTCTAAGCACTGGCCAGTCTGTTGTGGGCATTGTTTTCTA830.17888702099228415No Hit
CTGTAGGGATGGGAAAAGATTTGATAACACCAATCAACAGGAAGCTGGGGTG810.17457649036596407No Hit
TTTGCAGGGACCTAAGAACAGCTAAAAGAGCACACCCGTCTATGTAGCAAAA790.17026595973964395No Hit
GCTGTAGGGGTGTTCATTTTTTACTACATTTTATTAAAACCTGCTTTATACT790.17026595973964395No Hit
GTTTAGGGGAGAGCACAGCTTGGTTTTATACATTTTAGGGAGACATGAGACA780.1681106944264839No Hit
ACCTCACAGGCCCACCCCCTGCCGGGCATGCCGTGGGATCATGGGCAGGGAA780.1681106944264839No Hit
TGCTTTTGGGAAATTATTTCAACTAGAAAGCAAAAATAGGTGGCTTCGACAG780.1681106944264839No Hit
CATGCAGGGGACTATACAAATTGTAAAACAGATACTATAATATTTCCTTTAT780.1681106944264839No Hit
AAATACGGGCGCTGATCTGGATGTGGGGTGCCAAAGTACCCTTGTGATTCAG780.1681106944264839No Hit
TATTATGGGCATCACAAATCCAAGAATGTTTGCATCCTAAATGCTAGTTTGC770.16595542911332387No Hit
CACAGCGGATTTTGTGCAGTGATTATTTTTTAAAGTCTTCTTTCATGTAAGT770.16595542911332387No Hit
AAATTAGGGAATATATATGTACCTAATATTGGAACACCAAAATATGTAAATC770.16595542911332387No Hit
TGCTATGGGTACATCTGCAATATTGTGTTTTTAACGCCAGCACCCAGAACTT760.16380016380016382No Hit
TGTAAAGGGATCTCATGGTGTGCGATGAGCAAACGCCTGATTATTCATTATA760.16380016380016382No Hit
AGTGTTGGGGGTTTGATTAGATTTTTTAAAAAAATATGACTTCATCTGTGAT760.16380016380016382No Hit
ACCGCAGGGATGTGTGAAAAGGACGCAGACTGAAAACGTTTTGGTTTTGTAC760.16380016380016382No Hit
ACCATAGGGGACCCAGAAGGATGCCACCAGTTTTTCCTTGAGGCCTAGGATT750.16164489848700375No Hit
GGGACTGGGATGCATGTGTCTCTGTGTATATGGCATATACCTAGTAAGTATG750.16164489848700375No Hit
GTATATGGGATTCCACTTTAGGGAAATTCTCCTTTAAATGGGTCAGAAAATA740.1594896331738437No Hit
ATCAAAGGGTGTTCTGTGTCAGTTTCCTCTGCGCCACGTGCTTTCACAGTTT730.15733436786068367No Hit
GTACCAGGGGCAACGTCTTAAAAACAGGCGTGGTGCGCTGCTGCGTGGGACA720.1551791025475236No Hit
CTTATACACATCTCCGAGCCCACGAGACTTTCCGAAATCTGTCTCTTATACA720.1551791025475236No Hit
AGAGTAGGGAGTTGCAGCTGATGCCTGAATGTATCCTAGCTGACAAATTATT720.1551791025475236No Hit
ATGATAGGGAAGTATACCAAACAGAAGTAAACATATTCACAAGAAGACAATC720.1551791025475236No Hit
CTCTGTCGGGGATATACGGTATCAGAGACTGGTGGTGGAGCTGCTTATCAAA720.1551791025475236No Hit
ATTAAGGGGCAGGCACCGCCTCCAGGACAACGTGCGCGAGGACCTCTATGAG710.15302383723436355No Hit
ATCGCCGGGTATATATTGTTGTTATCCTCATTAGATTAATAAGGAAACTGAG700.1508685719212035No Hit
TGGTGCGGGGGTGGCAGGGCCAAGGTCCCCACTTTCTTGCTGACTGTAAACT700.1508685719212035No Hit
ACTCATGGGAAATGGGTTACCTCCAAATATGAAAATCTATAACAACCTATGG700.1508685719212035No Hit
AAAAGCGGGAGCCATCCCCTCCTCAGCAAGGCATCTACCGGCCCCTCACAGA700.1508685719212035No Hit
GACTTGGGACATATTTCAGTATAGTAAAAAGAGGAAGTCTATCACTGTAGTG700.1508685719212035No Hit
GGATATGGGAGTTTGATTTTTCTTTCCATATTTGAATTAATTTTTTCTGTTT700.1508685719212035No Hit
ATGTATGGGGGGCAGAGGTGCACTTTATGAAACTGGCACTTGACTGAACAGA690.14871330660804347No Hit
TGCAATGGGAAATATTTTATTTTTTATTTTTATTTTTGAGACAGGGTCTCAC680.1465580412948834No Hit
GTCTGGGGGCAGGCCCCACTGACATTAAAAGAATACTAAAGGAATGTCACAA670.14440277598172335No Hit
GGGCATGGGTATTTACTCAAAACATATGCCAAATTTAGTGTTGAAAGGCTCA660.1422475106685633No Hit
ACTCACGGGGAGACAAAGGGCCTTATTCATAAGTGGACAGGTTTCCCCATAG660.1422475106685633No Hit
CATGGTGGGCTGCAGAATTCCTCAGTGTTTGTTTATATGAAAGTTCATTGTG660.1422475106685633No Hit
CATCTTGGGAAGTGTACAGTATTTACCTTCCTTTGTCTTACATTGGCTTTTT650.14009224535540324No Hit
GTACTTGGGTACCAACAAGAAGGGAATGCAGGTAGTTGTTTAGGAGATGGTA640.1379369800422432No Hit
TGACGGGGACAGCAAGACGAGAAGACCCTATGGAGCTTTAATTTATTAATGC640.1379369800422432No Hit
AGCATAGGGATTTTGGAAGCAGCTGCTGGTGGCTTGGGACATCAGTGGGGCC640.1379369800422432No Hit
AATAGAGGGATATATAAACACTCCACAGTGTTTATATTGGGAAGATATTGGG640.1379369800422432No Hit
GGTTTAGGGATATCCAAATTCAACTGTTTGGTTCACTCTTGGTGCCTCATAT640.1379369800422432No Hit
GTTCGCGGGTCCTTTTAAGAATATGGAGATGAAGATTGTTTTCTCCAATTTT640.1379369800422432No Hit
GTATCTGGGAGTGAAGCTGATGCCTAGGAACTTTTAAAGGGATCCTTTCAAA630.13578171472908315No Hit
ACAGCTGGGGTATCTTTGCTGTAAAGTGCAAGGAAATTTTAAATTCTGGGAC630.13578171472908315No Hit
TAGCTTGGGAACTTTGAGCTGAGTGAGGGGAGAGGGGTAAAGAGAAAGAAAC630.13578171472908315No Hit
TAATATGGGGCAATAGGCTATAAGGAATAGCAGGAGAAATTTTACTGAAGTG630.13578171472908315No Hit
CTTGTAGGGATAGACAAGTGGGAAAAATGGTGTTAATACTTTCTAATATTTC630.13578171472908315No Hit
CATTCTGGGGTATTGCAATGGTATGAATCTGCTCATGTGGAGACTGGCTATA630.13578171472908315No Hit
AGTTAAGGGCACGACGCTCTTCCACTCTACGGCTGGGATTGCCATGGTTGAG630.13578171472908315No Hit
GTGCTAGGGCTAATTAACTGTTGATGAGCATTTTGGATATTCTAGGAGAAAG620.1336264494159231No Hit
GGCTATGGGTACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACT620.1336264494159231No Hit
GAATTAGGGGGTTTGCTGTGGAGGAAATAATGTACTAGATCATTGTCACAGA620.1336264494159231No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTTCCGAA620.1336264494159231No Hit
TAATTAGGGATTTTTTAGACAGTTTGATTATTCTTAAAAATTGAAAACATAA620.1336264494159231No Hit
CTCACCGGGCGCTCTTCCGATCTTCGATTGGGCCGATCTTGGTTAGGGATTT620.1336264494159231No Hit
TAACCAGGGTCCATGGTTGATGTTAAAGTGGATAAGATAAAAGGAGGAAAAT610.13147118410276304No Hit
GTCACCGGGTAACAGCCCCAAGACCAGCCAGAGGGGCTCTGATGATCAGCCC610.13147118410276304No Hit
TGCAATGGGGAAGGTCATAAATCTTTCAGAGTGACATCACCAACTGTACTGC610.13147118410276304No Hit
TATCGGGATCTTGATCTGTTTCCTCTATCTTGTTTCAGTGCACACGCTTGTG610.13147118410276304No Hit
GTATATGGGTCTATCAAGAGTGCTGAATTTCTGCATGTTGAAAGACTTAGTG600.129315918789603No Hit
GAATAGGGGCGATCTCAATGCGGGCTTCACTGATGCTTGTCTGGTAATAATC600.129315918789603No Hit
AACCAAGGGATAAACAATGTTAAATAAAGGTAGACTCAGTCTTTAAGATATT600.129315918789603No Hit
CTTTTAGAGGGGACCATATCGATTATAAATGTCTCTCTGTAAATTATAACTT600.129315918789603No Hit
CCTAACGGGGGGGGGTGGCGCTGGGGACAACCAGAAAGTTTCAAGCGACAGG590.12716065347644295No Hit
CGACATGGTATTCAGACTTCCGATGGGCTTATCTTGTCCCCTCCAGGTACAA590.12716065347644295No Hit
TACTATGGGATAGGAGATTCTGCTTATTGATCGGCTTGTTGCCTGTCTGCTC590.12716065347644295No Hit
TTATGAGGGCATTCTGGATTATTGTTCCAGCATTGCACAGTCCCACTAAACC580.12500538816328288No Hit
GAGCTAGGGAATGCCCCAACTAAATACTACCGTATGGCCCACCATAATTACC580.12500538816328288No Hit
CGTAAAGGGATGAGAACAAGAATAGAGTCATCATGTATTCTTTGTTATTTGC580.12500538816328288No Hit
TGTCTTGGGGCTTCTGGTACCTGATTTCCTGACATTGCTATTTATATGATGC580.12500538816328288No Hit
ATACAGGGGGTGCCCTTGGAGGCAACCTCCCATTTTAGATGTTTAAAGGACC580.12500538816328288No Hit
GTTTAGGGATGATGAAACTTCGGCTCACTCCTTGGCGCCTGCCTGATCCTCC580.12500538816328288No Hit
GCTAACGGGTATCTTCTCACATTCTGTATACTGTATCCATTTTCTAGAGAAC570.12285012285012285No Hit
AACGATGGGATCTGTAGAGGTTTGTTGTTGTTGTTGTTGTTTTTGAGATGAA570.12285012285012285No Hit
TACATGGGAGTCATGAACCACCACACCTGGCCTACATGATTTTTTTAAATCC570.12285012285012285No Hit
TAATCGGGTGATCCAGCAGTTCTATGCCTATATACATGCCCAAAGTAATTGC570.12285012285012285No Hit
CCAGCGGATTAACATTGGAAAATAGACATAATCTTTAATTTTTAAAATATTT570.12285012285012285No Hit
CAACATGGGAGTGCTCTGAGCCCTAGGATTCATCTTTCTTTTCACCGTAGGT570.12285012285012285No Hit
TAATAAGGGAGTGTAATTTCATTGCCTTTTAAAGGCTAGACATAGCTTTTGA570.12285012285012285No Hit
TACCCAGGGCAGATTACTCAGTGCTATAATTTTCTTTTAGTTAAACAAAGGG560.1206948575369628No Hit
CTCTATGCGGGCAACAAAATATGTTTTTACTGGATAAAAACAAGTTTTGTTC560.1206948575369628No Hit
CTAGCCGGGATGTGACAATTGTCAAAAAATGCACTAAAATGTAAATGGAGAT560.1206948575369628No Hit
CATACAGGGAAGACACAAATCCTCTGTATGCCATCTTGCATTGGCACTGAGG560.1206948575369628No Hit
GTTTTGGGGTGTGCATGAAATACTCTCTTTCCATAGCTAATTTACATTGTCT560.1206948575369628No Hit
CCAACAACGTGAAGACGGCCGTGTGCGACATCCCGCCCCGCGGCCTGAAGAT550.11853959222380275No Hit
ATGCATGGGATTGATGTGTTCCATGTCCAATTCATGAAAATAAACTGTTCTA550.11853959222380275No Hit
CATACACGGGACACGACGCTCTTTCGATCTAATAACGGGAACATTAAGCATG550.11853959222380275No Hit
AACTATGGGGGGCAGTGGAAGAGCTGCTGTATTTGAGGAAACTCATACAGTC550.11853959222380275No Hit
TGAAAAGGGATGTAAAGATTGTATTACATTTGTATACAAAATTTTTTTGCAT540.1163843269106427No Hit
GCATTCGGGCACCATGGCTAGTTTTTCCTCTGAAGATTGTTCGCCTCAAGGC540.1163843269106427No Hit
CACGCTGGGCTGTATCTAAAGAATAGATTAAAACATAGGTTACTATTTATTA540.1163843269106427No Hit
TTTCGGGGGCAACTTCAGGCTGATCAGCAGATGGGATGTGAAAAATACTACC530.11422906159748265No Hit
TCAACAGGGAAAGGGCTAGATTCTGGTAAATGGCATATATGAATGCACAGTT530.11422906159748265No Hit
TTTCAGGGGGCTCTTTTTCCGGCTGGAACCATGGAGGGTGTAGAAGAGAAGA530.11422906159748265No Hit
CTGCACGGGGAGACAGAAGGACCATATTCACATAACTTTTATTACAATATAA530.11422906159748265No Hit
TAAGCAGGGGAAATTCACCATGAAAAAACAAGAATAACAAATAGAAGGGAAG520.11207379628432261No Hit
TCACCGGGGGAAGCGGCGGTGCAGAACCCAGGGACCATGGGCGCCTCCAGGC520.11207379628432261No Hit
GTTAGGGAAGCACTAATATTCAGGCCTTACATTATACCAATTAAATCAGAAT520.11207379628432261No Hit
TCTGCTGGGATAAAGCATCATGTAAAGAAATCCTAACATTTAAGTGTAATTC520.11207379628432261No Hit
GGGTAACGGGCAGTATACAAGAGTCAATTGATTTCCTCTAGACCAGCAATGA520.11207379628432261No Hit
TAGATAGGGAAGACGAGAAGACCCTATGGAGCTTTAATTTATTAATGCAAAC520.11207379628432261No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTTTCCGAAATCTGTCTCTT520.11207379628432261No Hit
GGATCTGGGTAAACAGTTGTTACAATGTATTGTCAAAGACAAGCTCTCCTTG520.11207379628432261No Hit
CTAGAAGCGGGTCTGACTAAGGGATTCTGTTCTGGGGTCATTTTTGCAGGCC510.10991853097116254No Hit
AATGCAGGGAATGATATGAAATGATGAAATGATAAAAAGTGAAATGATTAAA510.10991853097116254No Hit
ACTGTTGGGGAAACCGGTTAAGGGAGAAATTTTAAACATGGCAAACTTAAGA510.10991853097116254No Hit
CAAGATGGGTTAATGGAGGATGTCATCTTTTCATAGATGCTGGAACTAGAGT510.10991853097116254No Hit
GCACTGGGGAAGGAATCTTTCTTGAAGGAAATTTAAATACAGAATGAAACAT510.10991853097116254No Hit
TATTGTCGGGGGGGTTTTGGCCCAGCCAGAGGACCCAGAGTTGAGCTTCAAA510.10991853097116254No Hit
TAACGAGGGGAGTTTCTGTTGGGGAGGAAGAAGGTGAAGGTGAAACTGGTTG510.10991853097116254No Hit
GTATATGGGACCAATGGATACATAAACTGGAGCCCTAGAAGGATGAAAAAAG500.1077632656580025No Hit
TACACAGGGTCTCCATCTCCTGGGCTCGAGTGATCCTCCTGTCTCACCTCCC500.1077632656580025No Hit
TCCTGAGGGCATACCGTTTACTAGCAAATAAAATTTTCTGATCTGTAAAAAC500.1077632656580025No Hit
TATTTAGGGAGTGTACATTTCAGATAATTTCCTTCAGTCTCCAGATAGGAGT500.1077632656580025No Hit
TTATAAGGGAGAGATGAGCTTTATAGCTTCTAAAATAAAACCCTAACTTTTT490.10560800034484245No Hit
GACTCCGGGCAGTCAACTTCTCAGAGTTTTCTAAGAAGTGCTCAGAGAGGTG490.10560800034484245No Hit
TAGTTAGGGCAGAAATAAAATGTTTATGCTATTGTTCACTACTGAAATGATG490.10560800034484245No Hit
GCTGATGGGTGGCTTTTATAGTGACAGGCAAAGAGGAATTTATTGAGATCAC490.10560800034484245No Hit
TCGCCCGGCCAGCCGCCCCGTCCGGGAGGGAGGCGAGGGGGTCAGCCCCCCG490.10560800034484245No Hit
GACCTAGGGGTGGATGGCCCCTCCGGGAAACTGTGGCGTGATGGCCGCGGGG480.10345273503168241No Hit
GGTAACGGGACCTGTATTTTGAGGACAAGAAAGCTGAATGAGGGCAGAGGGG480.10345273503168241No Hit
TACGGTAGGGGGGCTCGGGGACGCGCTTCCTGGTGCTGGGCCCTGGCGCTGC480.10345273503168241No Hit
CGTCTAGGGTATATGGAACTTTATTTTCAAATCTGCTTGTCTTCTGGTCATA470.10129746971852234No Hit
AAGTATGGGGTGTATTCTGTGGATTCTATATTTCATATTGAGATCAGCATTC470.10129746971852234No Hit
GGAAACGGGATGAGACATCTATGAGAAACTTTTGGAGCAGTGCACTCACTCA470.10129746971852234No Hit
ATGTAAGGGGGGTACACACTGAGGCCTTGATGATAACATTGGAAATGATGTG470.10129746971852234No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)