FastQCFastQC Report
Sat 31 Dec 2022
SRR5847018.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5847018.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences83085
Sequences flagged as poor quality0
Sequence length52
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTTCCGAAATCTCGTATGCCGTCT8431.0146235782632245No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTTTCCGAAATCTCGTATGC6850.8244568815068906No Hit
AGAGAGGGTGGGTCTCTGAGTCTGAGAAGGGATACCGCAGCATTCACGTCGC2580.3105253655894566No Hit
AGGTTAGGGGCACTCGCGCAGACCTAGCGCGTCCAGGTGGGAGGTTAGTGTG1670.2009989769513149No Hit
AACGGCGGTTCTGATCGGGGCCACGGTGATCGGCCTGATGAACCGGTTCGCC1660.1997953902629837No Hit
CTTATACACATCTCCGAGCCCACGAGACTTTCCGAAATCTGTCTCTTATACA1510.18174158993801529No Hit
TGTTCAGGGAATTCTGTGGGCGTGTACTGCTGTGATAAGATGTTTGTTCTCT1490.17933441656135285No Hit
TAACCGGGATCTGAGATTGTTCTAGAAACTGATGAGGAAAGAGTCGTACTAA1480.1781308298730216No Hit
ACGGGTGGGAGAGATGGGGTTCTCTTGCTGTCTCTCTGGCTGATTCCACGCA1450.17452006980802792No Hit
GCAAGGGGAAAGACAGAGATGGGAAAACAAAGCAGGTATCTAGTGGATTAGA1420.17090930974303425No Hit
AATGGCGGGAGTTGCAGGTAATTTTTTGTCTGCTAGTAGTGTTCTAGATTAT1360.1636877896130469No Hit
GTATGCGGGAACCTCTGCCTCCTGGGTTCAAGTGATTCTTCTGCTGAGGCAG1360.1636877896130469No Hit
ATTCACGGGATATTAAGACAGTGTACAAAACTGTAAATAAAATGTGTACAGT1350.16248420292471566No Hit
GGTTACGGGATTTTATTTGAATATAAGTTTAAGGAGTGAGAATGGAGAGTTG1350.16248420292471566No Hit
GCATATGGGATTCATAGTAAATGCCGTATACAGATGTATCATCTTTAATCTT1350.16248420292471566No Hit
ATCAAGGGGATACAAGAGCAAAATTCAAGTAATACAAATATTTAACATTTTG1320.15887344285972196No Hit
ACTATTGGCTACCGAGGGGAATATAAAATTGTTTAATCACTTCCTGCATGTT1320.15887344285972196No Hit
CTTAACGGGATTGAATGAATAAAGAATATGTGGTATATATACACAATGAAAT1310.15766985617139073No Hit
ATTGAAGGGAACAAATAGAAAGAAGAAAGCCTGTAATCTGTTTCAGAATGCT1280.15405909610639706No Hit
AAATAAGGGGAAAGTATCTTGGTCTAGTGCAGTATTCTGATTTTCTCTTTTT1260.1516519227297346No Hit
TTGTACGGGGCTGCCGGGAGCTTTACCCAGCGTCCTGTGAGCCTCAAAACCA1260.1516519227297346No Hit
TTTCTGGGAGTGATAAGGAAGGAAAAAGGTAAAAATACAGTATACTTCACGA1250.15044833604140337No Hit
ACAAACGGGACACGACGCTCTCCGATCTAGCTCGGGATTCTAAGGATATGCT1250.15044833604140337No Hit
TTCAGTGGGTCGCTTTCATGCATGGAGGAGGGGAATCTACGCAAAGAAAGGC1240.14924474935307216No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTTTCCGAAATCTGTCTCTT1230.14804116266474093No Hit
GTTGACGGGACTCACTCGTGTCTCGCTACCAGCTCCCCGCTGCCCTGCGCTC1210.14563398928807847No Hit
ATAATAGGGAAGGACAGTTTAACATTACAAATGCTATAAATACTTCATAATT1210.14563398928807847No Hit
TTCATAGGGACTTTCAGTCAATATGTGTCTTTACAGGTAATGTGAGTTTCTT1200.14443040259974724No Hit
GGATGGGAAGAGTAAAGTAGACACTAATAAAGCACACCCTGACAATAAGGCA1200.14443040259974724No Hit
ATTACGGGAAGAAATGAAGATATGCATTTCCAGTTCCAGAGAGAAATTCACA1200.14443040259974724No Hit
GGTCTGGGAGTTTGAAAAGCCTAATATTTATTTGTATGTCAATATTTTTCAT1190.14322681591141603No Hit
TCTTGCGGGGATATTTCAGATGGGCTTTTGATGCACTGTTGCCAAGGAAGGC1180.1420232292230848No Hit
TCAAACGGGAGCTGCTCTGCAAGGTTGAGGGGCCATCACAGGGCGCCTGGCT1180.1420232292230848No Hit
ATTGCAGGGATTTTTGATGATGTTAGCAGCTGTTGATGCTCAGTGACTAGAT1180.1420232292230848No Hit
TTTATTGGGAACATATACTTGGCAATAAGTCACAAAAACATTCGTATTTTTT1160.13961605584642234No Hit
GGGCGGGGAAGTTGCTGGATAATACATGCACTATGATCCTATTTCTGTAAAA1160.13961605584642234No Hit
ATATCAGGGAATCAGCATGCTCCTAGTGAAAAAAAGAGGAGATATAATAACA1160.13961605584642234No Hit
GTCGAGGGATGTACTGAGCACCTACTAAATACCCAAAATTATGCTAGAGTGG1160.13961605584642234No Hit
TTCGTTGGGAGCTGAAAAGTAAATTGCCTTCTAGACACTGAAGGCAAATCTC1150.1384124691580911No Hit
ATAACTGGGAAACTTTAGTTTTGTCAAATTTTTTTCTATTGCTTTTTTGGTC1150.1384124691580911No Hit
TGCAAAGGGAGAGGCTGCCCCGGAGGATGCCGCAAGCTCCCCCTAGCCTCTG1140.13720888246975987No Hit
CTGGCATGGGAAATTCCATAATGTGCCTTATCTTTTCATTGCAGGATGACAG1140.13720888246975987No Hit
CTGTCGGGGAAAAAATCTAGATGTAATCAAGAGCTGGATTTGTCTTCTGATG1140.13720888246975987No Hit
ACAGAAGGGACAGAAAACCACTGATAGTATTTGTTGCCAATGATAAAATTTA1140.13720888246975987No Hit
AATGCAGGGAAGTTATGGGCAAGTCACTGAACCATGGGCCATCAGTTTCATT1130.13600529578142864No Hit
TCGCGGGAGTGTTTGGTTTGGAAACCATCGCATATTAAATTATTCAGCAACT1130.13600529578142864No Hit
CTATGAGGGATCCAGCTGGGCCGGAAGCTTATCACAAAATACAATCGCCGGG1110.1335981224047662No Hit
TCTCAAGGGGACTGTAAGTCTGAACTACAGAGCCATGGTGCTTAACTGATAA1110.1335981224047662No Hit
TCACTGCGGGACCAGCAGCCAATCAGATAACTCCAGTCGTGTTTATACTTCT1110.1335981224047662No Hit
AGATCAGGGAAGAGACCTACTGTCCGAAAACATTGTTACATACATTATTTTT1110.1335981224047662No Hit
AGTAATGGGCATGTGTTCTCATGTCTTTTTGCAGATTTAAAGGTTTTATTGT1100.13239453571643497No Hit
TAATGTGGGTGTGCTCGGGAGGCGTGAGCCACCGCACCCGGCCATGTATACT1100.13239453571643497No Hit
CGTTTTGGGATGCCTCCTCTGGCTTCCTCATGTAAAGCAGTAGTAGTTCATT1100.13239453571643497No Hit
AATCGGGGGATCAGTGGAGTGGAAGGTGACTTGCTTGGCCCTTGTTCTTTTT1100.13239453571643497No Hit
GAAACTGGGATATAGGCAAATTTTCTCTTTACATGAAATGCTTACCTATATT1090.13119094902810374No Hit
GTTGTTGGGATCCCATTGTCAATACATTTTTTAAACATGTTTTAAAATTGAG1090.13119094902810374No Hit
ACGCTCGGGAACTCTTTTAATTTATATACAAAGCAAAGAACAACATTAATGG1080.1299873623397725No Hit
TCGTCCAGGGAGGCCCCAAGTTATTTTTTAGCAATCTTACGAGTGAAATGTT1080.1299873623397725No Hit
GCTTAAGGGATATTACAGTATGTTAAACATATGTTTTCTCCAAGCACCACCA1080.1299873623397725No Hit
TAACTTGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1070.1287837756514413No Hit
GTAACAGGGAAGCCATATAGATTCATTTAGCTTCAGCCTCTGCAGCTGTAAT1070.1287837756514413No Hit
TGGTCAGGGATTTGACGCTACTGTAAAACTCCTTGGCCACAAATTTGGAAAG1070.1287837756514413No Hit
ACTGCAGGGATCTCAGAAGATGGCGTACAAAACTATTTTTCTAACTATAGCA1070.1287837756514413No Hit
AAGTCCGGGAGTTGGGCCCTGTACCATCCCAAGTCCTTTGTAGCTCCTCTTG1070.1287837756514413No Hit
GTAATTGGGAGGGAAAGACTGATGAAAAGTAAAATGGACTACTTTTGTAACT1060.12758018896311005No Hit
AAGTCAGGGGACGCTCTTCCGATCTGTTTATGGGATTTCAATTGTACTTTTT1050.12637660227477884No Hit
CTCAAGAAGGGAGAACATGACTTTTAAAAATGGAAGGGATTAAAAATCCTTT1050.12637660227477884No Hit
AGTTCGGGACTTCTAAATGGAAGGGAAAAAGTTACCCACACAAGTTAGAAAA1050.12637660227477884No Hit
ATACTAGGGACGCCCCAAATAGCTTCCGTTTGGGCCCTTACATTCACAGATA1050.12637660227477884No Hit
TTTAGCGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1050.12637660227477884No Hit
TTTGGCGGGATGGCTTTATTTGTTTTTTTTGTTTTGTTTTGGTTTTTTTTTT1040.1251730155864476No Hit
GTTTATGGGATTGCACTCCAGCCTGGGTGACAGGGCGAGACTCTGTCTCAAA1040.1251730155864476No Hit
GCTGCAGGAAGAACTGTCCATTAAGCCTAATGGAATACCAAACTGTCTTCTG1030.12396942889811638No Hit
AAAGTCGGGACTGGATATCAAAGATTATTTGGAAAAGAAAAATAAAATCTCT1020.12276584220978516No Hit
AATGCTGGGAGAGCTCTAAAACACAAAGTATTCTGAAGAAGTAGGTAACTGT1020.12276584220978516No Hit
CCACATGGGCAAGTATGGCTTTACTTGGTGACACTGGCCATAGCTAAGTTAA1010.12156225552145393No Hit
TTGGTACGGGGTTTTCCTGGAGCCATTTGGTATGAGGTATTAATTTAGCTTA1010.12156225552145393No Hit
TTAATGGGGCTAGGAGTTTGAGGCCAGCCTGGACAACAATGTGAGATTGTGG1010.12156225552145393No Hit
AACACTGGGAACTTCCAGTTGGTTATATGGAAGATATGTAAGATAAAACTGA1010.12156225552145393No Hit
CTTATACACATCTCCGAGCCCACGAGACTTTCCGAAACTGTCTCTTATACAC1010.12156225552145393No Hit
TATATAGGGTGGCATAAATCTAAGAAGAAGGCCTTTACCAAGTACTGCAAGA1000.12035866883312271No Hit
GAAGTGGGGCAGACCCTGCTCGCCGCTCCATATGACGGCGGCATCGACAGCG1000.12035866883312271No Hit
GTGTAAGGGAAGGATGTTTTTCTTTATTTTTGTTAATATTAAAAAGTCTGTA1000.12035866883312271No Hit
AACGTCCGGGATTAGTATCAAAGATGTTATGGTAATTCAATATGTACAGTTT1000.12035866883312271No Hit
AATACGGGGCACAGCCTCGTCTGGAAAAAGGACCAGGGGTCCCGGAGGAACC1000.12035866883312271No Hit
CCTTAAGGGAAGCCATCGTCATCATAGGAGACGACAGCTCTGTGCCTGTTAT990.1191550821447915No Hit
CAACATAGGGAAATATGCATTTCTAACCTTAATCGGTATGGTTTCCATTTTT990.1191550821447915No Hit
TACTGTGGGACCCAGAGGCCTGCTCTCCTCACACATTGTGTGGTTTGGGGTT980.11795149545646025No Hit
TTGACAGGGGATCTCCTGCAGGGAAGTAACAAGCTTCTAGAGGGCCCGGTAA980.11795149545646025No Hit
CTTATACACATCTCCGAGCCCACGAGACTTTCCGAAATCCTGTCTCTTATAC970.11674790876812903No Hit
TACGCAGGGACCGAAGGGCATCTTATAAGGGAGGTGGGGGAGGCGGGGTCAT970.11674790876812903No Hit
AATATAGGGATGACACACTAGGGAGTTGTGGCAGGCCTGAGAGTCTCCTCAG960.11554432207979778No Hit
CAATGAGGATTATTTGTGAGCTTTAATAGGCCAGTGAGTCTTGAAGAGAATA960.11554432207979778No Hit
AAGCTGGGGATAACTTGTGTGATTTCAAATTAAACTTGCATTATGTGTTAAT960.11554432207979778No Hit
GAGCACGGGAAGACCATCTTATTCATTTTAATAGTTAATTATTGAGTTAATT960.11554432207979778No Hit
ACGCAGGGGACATCAAAGAAAGAAAAATATAAGCCAATACCCCTGATAAACA960.11554432207979778No Hit
CGTAATGGGAGTATATTTGAAGAGATTTCCAAAAAATATAAACAACATCAGC950.11434073539146658No Hit
ATTGATGGGCAAGAGAGAAGATTCCAATTCTAAAAGTATTCGATGCAGAATA950.11434073539146658No Hit
GTGATGGGATTGCTTATTCATAAGATTTTAAATGAGTTAAAATATTTAAACA950.11434073539146658No Hit
ACTGCGGGGACTCCAAACCCTGTGCTCCTAGCCCCAGTGTTGTTCTTTCTAA940.11313714870313533No Hit
GAGTACGGGATCCACTTTCTGTATCTATAGATTTGTATATTCTGGACATTTT940.11313714870313533No Hit
GCCAGCGGGTCCTGATGAGAGAGAAGATGGTGCCAAGGCTGTCTTTGTATAA940.11313714870313533No Hit
CATTCTGGGGTTCCATTGGTCTATGCTTCTATTTTTGTACCAGTACATTGTT940.11313714870313533No Hit
CGTGGCGGGATTGCTAGAATAGCTAAAATATTTTGGAGAACTAGAACAGAAT940.11313714870313533No Hit
CATACAGGGATAGACTTTGATTTTAGCATCCTGTCTTCCATTAAGTTTCCCT930.11193356201480412No Hit
ACAACAGGGGATCTACGGTTGGATTGGTACTTCTGGTTGGGGCTCTCTTTAC930.11193356201480412No Hit
ACACAGGGCGATCTATATGCGGGATAGGAATCCTTGTGATTTTTTGCACATT930.11193356201480412No Hit
TGCTCCGGGATAAGGCTCATGATATAGGAAAAGGAATATAGGGCTAAAAATA930.11193356201480412No Hit
AGTAAAGGGACTAAGATATTGCTTAGCGTTAAGTTTTTAACGTAATTTTAAT920.1107299753264729No Hit
AAATGAGGGAAAGTTTGCCAAGAGAAGTATTAAAATGCACTGTATATTTTAT920.1107299753264729No Hit
GATGCTGGGATATCAAGGATCTTTTAAATTATGCCACTACAGGATGTATCAT920.1107299753264729No Hit
AAACACGGGATTCGAGAGCTGCTGCCAAAACGCCTGGCGCCGCCACCGTCGG920.1107299753264729No Hit
ATATAAGGGACTATGGGCAGTTCACACAAGGCAAAAACTATTGAACAGTTGG920.1107299753264729No Hit
CTAATAGGGAGAGCCTGGCCATGGTGCAGGCTTTGGGTGTTCAGCACCTGAG920.1107299753264729No Hit
AGTAAAGGGAGGTAGCAGTAGTGTGTGCTGCTATTGTGGAATATACGTGTGT910.10952638863814165No Hit
GTAACTGGGGATCTGCTACGGGGATGCAAGAACTTATCTATAAACACATACA910.10952638863814165No Hit
GTTGCGGGAGATTTTGGAAATATTCATCCTGCAGGATTCTACTTGTTTTATT910.10952638863814165No Hit
ATTTCTGGGAATAAAAGGTATAGGTATTTATATATTAATATTTAAGTCATAT900.10832280194981043No Hit
ATTAACGGGAAAAGCACTATGGCAGATTAACACTTTCTCAGACCTTGTAATT900.10832280194981043No Hit
AACAGTGGGATGGCCAGCAATCCTGTTCCCCCTCAGCATCCTGATTTAATTT900.10832280194981043No Hit
GTACTGGGGAACATTTAGCTGTATGATTTTGTCCTCGTTGTTGCCTAAAACG900.10832280194981043No Hit
AACTGGCGGGACCCACTTAAATGCTTAGCTTTTCTTGTAATTTCATCAAGAA900.10832280194981043No Hit
ATCTTAGGGACTAAAAATTTGTTCCTTTTTCACTATGGGCAGTTCACACAAG890.1071192152614792No Hit
GAACTAGGGATGCCTTTATAGGGTCAGGCCTACAATGAATAGGTATGGTGGT890.1071192152614792No Hit
GCAGTTGGGACTCTTACTAACAATTTTTATACAGAAAATGAGTCATTTTGGA890.1071192152614792No Hit
ACCTGGGGGGAGCTTCCTGTCCGGTGTTAGAAAGTAACTAGTTAAAAGTTCA890.1071192152614792No Hit
GTGCGAGGGAGAATTGTTAATACCTTTACCCTTCTCTTGAACACATCAAAGG890.1071192152614792No Hit
GGTATTGGGAATAGCAACATGACTTTTGGCAAATTACTCCAACTGAGTGTCT890.1071192152614792No Hit
GATCGGGAAGATTTGGAAGCAACCTAAGTGTCCATCAGTAGATAAATGGATC890.1071192152614792No Hit
TTTCAGGGGGCTCCCAGTGTTTCCATTTCATGTTTTAATTCTCATTTTAAAA890.1071192152614792No Hit
GTTAAAGGGACCAGTAATAGTTGCATTGATACTGCTGGCACCAGTTAATTCT880.10591562857314799No Hit
AGTCTCGGGATTATAATCAATTTGCAAAGAAAATTGCTGAACCCTTGTTTTT880.10591562857314799No Hit
ATTAAAGGGAGGAACTTATGTGTGGGCTACATAAAATTTGGTGTTGAGGGAG880.10591562857314799No Hit
AAACAAGGGACTGTGGTTTTGATTTGCATTTCTCTAATGATCAGTGATGTTG880.10591562857314799No Hit
CAAGTCGGGATACAAATGGCCAATAAGCACATGAAAACATGCTCAGACATCA870.10471204188481677No Hit
CTAGCTGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA870.10471204188481677No Hit
AAAAGTGGGACAGCTGAGCAGTGTTTCGGCGATGGCTTGAACTGGGCTGGTT860.10350845519648552No Hit
TAATGGGAAGATATATTCTTTTTGGTTTTAAAATGCAAAATCTTATTGGCTG860.10350845519648552No Hit
ACCGTAGGGACGTTTAATTTAACAAACTATAGACCAAATACTTCAAGTTTTT860.10350845519648552No Hit
CTTCATGGGGGAGGCGAGGACTAGATTATTCTCTTTAGTTCCCCCAAATTTT850.1023048685081543No Hit
TGACACGGGACTGCAAAAACATGCCAAATTGTAAAGACCATCAATGCTAGGA850.1023048685081543No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTTTCCGAAATCCTGTCTCT840.10110128181982307No Hit
AGTTTTGGGAGAGATGATTTAAAAAGTTCAAACAGACATGGTCATAAAAGGA840.10110128181982307No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)