FastQCFastQC Report
Sat 31 Dec 2022
SRR5847013.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5847013.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences59862
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTTCCGAAATCTCGTATGCCGTCT4450.7433764324613277No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTTTCCGAAATCTCGTATGC4170.6966021850255587No Hit
TGTGGTTGGGGGCACAACTGAAGCAGCCTTGTCCCTCATTAGATGATCTTGC1240.2071430957869767No Hit
AACCAAGGGAGGCTTAGAGAGCATTTATTTATCTGGCAGCTGCAGCATTTTG1070.178744445558117No Hit
ACGCTGCGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1040.17373291904714178No Hit
TGTAACGGGATGATTTGGCTCTCTGTTTGTCTGTTATTGGTGTATGAGAATG980.16370986602519128No Hit
GACCTAGGGACCAGTCATAGCATTTACTTCTGCTTCTCCTGGATTATGTGCT940.15702783067722428No Hit
CTTATACACATCTCCGAGCCCACGAGACTTTCCGAAATCTGTCTCTTATACA930.15535732184023254No Hit
TGAACGGGAGTGTGGGTGTGGCGGTGCTGGAGCTGCTCAATTTCCCGCCGCC910.15201630416624903No Hit
CTACATGGGCCGATCTTGGTGAGGGCCTTGACAGTTCTTTGCACACAAGTTT870.14533426881828204No Hit
AAGTACGGGGCGAACGGCGAGCAGCGGCGGCGGCGCGGGAAGTGCAGCGGAG860.1436637599812903No Hit
TAGTGCGGGAGGGATCCTGCCCCAAATGCGGTCGGAGCGCCAGTGACCCTGA850.14199325114429853No Hit
CTACAGGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC840.14032274230730682No Hit
GGTCATGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC840.14032274230730682No Hit
GACTACGGGTGATCTCGGTTTGGGAGACTTAGGGTTTATCTATTTTTTTTTT840.14032274230730682No Hit
ACAATGGGGAGAAGGTCACAACACAGGCCTTTTTCACTCAGCTTTGTAGGCA830.13865223347031508No Hit
CCATCAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC810.13531121579633157No Hit
TTCCGAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC810.13531121579633157No Hit
AAACCTGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC800.1336407069593398No Hit
AGGTCTGGGATACTTACTTGTATCCTGGCTTTGGCTAGCTAAAAATTCCCCA790.13197019812234806No Hit
TGGTCTGGGATCTTTGCAGCAATTCCAGTAATAAAAAAATGCCCATTTCTTT780.13029968928535632No Hit
ACTGAGGGGATCCGGCCTGAGGCGCAGCGGTCGCGTTAGTTCGGCCCAATGG750.12528816277438107No Hit
TATCCAGGGCATTTCACATGAACTAACCCCATTTAAAAGAATACATTTTCCA750.12528816277438107No Hit
CTATACAGGGGACCCAACACCGGAAGGCTCAGATTCATGAAACAAATATTAC740.12361765393738933No Hit
CAATTAGGGTAACTTACACCTTTAAAGAGAGACACATAGAGCAGGAATTTTT740.12361765393738933No Hit
GTAGAGGGGATATCATACAATGGAATACTATTCAGCAACAAAAGGGAATAAA730.12194714510039759No Hit
TTACCGGGGACGAGAGCTTCCCACCTTCTCACCTACACCTGTGATCTGCCTC720.12027663626340583No Hit
AGACCAGGGATGCACAAAGAGACCGATGTGAAACACAGTGTCAAATGAAGAA720.12027663626340583No Hit
TGAGTAGGGAGGGCTCCTCCCTCCTACCTCAGGCCTGATCCATCGTGCCCTT720.12027663626340583No Hit
TTTACTAGGGAGCTGATGCCTGAATGTATCCTAGCTGACAAATTATTGATTA710.11860612742641409No Hit
AGTACGGGGGCGGCGGTGGTGGCGGCTGTCAGAGCTGGAGAGCGGCCGGCGG710.11860612742641409No Hit
GCTTTTGGGATAATTATTTTCACTTTTTAATTGCATAAACACACACACATAT710.11860612742641409No Hit
TAGTGACGGGAGGCACACTTAATACATTTTTTTTCAAGCGAAAGTGTCAGCA710.11860612742641409No Hit
ATGGCAGGGAAGCTAGAAATTCATGACAATGAGTATTACTATTTGCCTAATG710.11860612742641409No Hit
CAGCCTGGGTGACAGAGCTCGACTCCATCTCAAAAAACAAAATAAACAAAAA710.11860612742641409No Hit
ATCTTAGGGCCAGCAGTGATGTCAAGTGCCCAGTGCCTTGGGGAACCGGCTT710.11860612742641409No Hit
TCAAAGGGGGAAATAAAAAAGTACAATATGTAGTATATTAGAAAGCAATGAG710.11860612742641409No Hit
TACGACGGGATAAGCAATGGCAACAAAAGCCAAAATTGACAAATGGGATCTA710.11860612742641409No Hit
GTGCAGGGAGATTAAGAAATTTGGACTCTAGATCTTGTTTATATAGTGAGTT710.11860612742641409No Hit
GGAAATGGGAATGTGGCACTTTATGTCTCCATTTCTTCCATTCTTTTCCTTT700.11693561858942232No Hit
TGTGCCGGGGCATACACATACATGCACACACACACGTTATCCCATGATGTTC700.11693561858942232No Hit
TAAAATGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC700.11693561858942232No Hit
GCAACTGGGAGGAAGTGATGGAGCTTTAGATTTTCTTTTAATATTGCTATTC690.1152651097524306No Hit
GTATTGGGGACCCTCTACAAGGCCTACCTGGAGTCCTTCTACAAGTTCTGCA690.1152651097524306No Hit
AACGAGGGGCAATAAAATCTGGGTTGATTTATAGTCCCATAGTTAATTCTTT690.1152651097524306No Hit
GGTCTAGTCAAGGGATAGTATTTTTTCTTTGCCTCTAAGAAAATACTTTCTT680.11359460091543885No Hit
TATTCTGGGCGATCTAAATTCGGGAGGATATAATGTGTTAACCTTTTGTGAT680.11359460091543885No Hit
TACTGCGGGAAGACTTAAAGCAGTCTTGTTAAATTGACATAAATGGTAAACT680.11359460091543885No Hit
GCCATGGGGACACCATTTTGTAAATGAAACTTAAGAATTGAATTGGAAAGAC670.11192409207844708No Hit
CTCACTAAGGGGGAATCTTTCAGTTATAATAATCATTGATCATATGCTAATG670.11192409207844708No Hit
GTAGTGGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC660.11025358324145534No Hit
CATAGAGGGAGAAACCTTGATTAGAAGTGGCGAAAGAAGGAAAGAAACTGTG650.1085830744044636No Hit
AAAACGTGGGGGGTTCTCTAGAAACAAGAGCCTGACTGGAAATTAAGTGAAG650.1085830744044636No Hit
CGGCGCGGGTACCAGCCAAGATAGATAAATATGCCCAATAAGTATAATTGTT650.1085830744044636No Hit
AGTACGGGGGTGCGTGGTGGATAAGCTTGATCTCGTCTTCCCTGAAGTCTGG650.1085830744044636No Hit
CCTCAAGGGAGTGTGAAACTAGAACAATAATACAATCTTGTTAAAGCTGTGC650.1085830744044636No Hit
CATCCTGGGGATGGATTTAATTCTTGATGCTGGTGCTAGAAGTAGGTCTTCA650.1085830744044636No Hit
ATCTATGGGACATTCTCTAAGCTCCAAATCAGATGTCGAGGGTAGGAGCACT650.1085830744044636No Hit
GTGAAGGGATAAAAGGATAGCACAAGAGATCCTTGTGGTGATGGACATATTC640.10691256556747185No Hit
CTGGCAGGGAATAAAGCTGGAATGAGACATAATTAAGCTAGTTCAAAGGAAA640.10691256556747185No Hit
TCAGAGGGAGTCCCTGTACTGAAGATATCAAAATGAATTTGACAATCTGGTA630.10524205673048011No Hit
CTCTATGGGCAGCTTGCGGGCATAATAAGCAACCTTTCTTCTCTCTGTTAAT630.10524205673048011No Hit
CTTGGAGGGAGGGCAGAGCCTGAGGATCTAAGCGAAGGCTTCCCCGGGTGTA630.10524205673048011No Hit
AGACGGGATTTCTGAATTATGAAAGCCTCTCTAAATCAGGAATTACACAGTC630.10524205673048011No Hit
CCGTTTGGGATATGACACTTGGTTATAACTCTTTAGTTGGATATAACATTAT630.10524205673048011No Hit
GTCTCAGGGTACTGACTTCAAGAAGGGCCTTGAGTAAGATATTAACCTTTCT630.10524205673048011No Hit
TGCAAGCGGGAACCACAGAATACACATTCTATTCAACAGCACATGGAACTTT630.10524205673048011No Hit
AAGTTTGGGAGGCTCAATTTTGCTGTGAACATAAACTTCTCTAAAAAGTAGC630.10524205673048011No Hit
TACCATGGGATAACTTTTGATTTCTAAGGAAAATATTAGCATACATTTCAAA620.10357154789348835No Hit
TCACACGGGTCTTGCTTGGGGGAGAAATTAAACATGTAAAAATAGAGTCCAC620.10357154789348835No Hit
CAGTTTGGGAAGACGACTCATATCACATTAATTTTAGAAATATTAACGTATT620.10357154789348835No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTTTCCGAAATCTGTCTCTT620.10357154789348835No Hit
TGTCCAGGGAGGCAAACTGGGAGGACCTGGTCACGCTAACAAATGGGAAAGT620.10357154789348835No Hit
CAAAATGGGATGTTAGAACAGGCTGTTCATTTCTCCTTTTGCATTGTCCTGA610.10190103905649661No Hit
CTGCTCCGGGGGGCATTTGAAATAACATACTATGGCAGCTCTGAAATTAGAT610.10190103905649661No Hit
CTAACGGGATTTGGGTTTGGGTATAATAAAAAGAGGAGTGCCATAAAAAGAA610.10190103905649661No Hit
AGCCACGGGAGACTATTTCTCTTTGGAAATTGATGAGGAAGGGAACCTGATT610.10190103905649661No Hit
GAAAGTGGGAGGGAAGTTATGAATTACTCCTTCCAGTAGTAGCTCTGAAGTG610.10190103905649661No Hit
CTTCTTGGGATCTCCAAAGATGTCATTGGATGTCATGTACCTACTAATACAA600.10023053021950487No Hit
GGCCGGGATAGTATAGTCCAACATTCGTTGATCCCAATACAGGCAAACAACC600.10023053021950487No Hit
AAGTACGGGGGTCTCTTATGCAAGAGCATGGGTCCCAAAAAGGTGGGGATGC600.10023053021950487No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)