FastQCFastQC Report
Sat 31 Dec 2022
SRR5847012.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5847012.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences59085
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACTTTCCGAAATCTCGTATGC4570.7734619615807734No Hit
CTTATACACATCTCCGAGCCCACGAGACTTTCCGAAATCTCGTATGCCGTCT4390.742997376660743No Hit
TGTGGTTGGGGGCACAACTGAAGCAGCCTTGTCCCTCATTAGATGATCTTGC1110.18786494034018786No Hit
TGGTCTGGGATCTTTGCAGCAATTCCAGTAATAAAAAAATGCCCATTTCTTT1010.17094017094017094No Hit
TGAACGGGAGTGTGGGTGTGGCGGTGCTGGAGCTGCTCAATTTCCCGCCGCC1000.16924769400016923No Hit
CTACAGGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1000.16924769400016923No Hit
AAGTACGGGGCGAACGGCGAGCAGCGGCGGCGGCGCGGGAAGTGCAGCGGAG980.16586274012016586No Hit
TATCCAGGGCATTTCACATGAACTAACCCCATTTAAAAGAATACATTTTCCA960.16247778624016246No Hit
CTTATACACATCTCCGAGCCCACGAGACTTTCCGAAATCTGTCTCTTATACA940.1590928323601591No Hit
GGTCATGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC930.1574003554201574No Hit
CTACATGGGCCGATCTTGGTGAGGGCCTTGACAGTTCTTTGCACACAAGTTT920.15570787848015571No Hit
AGGTCTGGGATACTTACTTGTATCCTGGCTTTGGCTAGCTAAAAATTCCCCA890.15063044766015063No Hit
CTCACTAAGGGGGAATCTTTCAGTTATAATAATCATTGATCATATGCTAATG890.15063044766015063No Hit
AACCAAGGGAGGCTTAGAGAGCATTTATTTATCTGGCAGCTGCAGCATTTTG890.15063044766015063No Hit
ACTGAGGGGATCCGGCCTGAGGCGCAGCGGTCGCGTTAGTTCGGCCCAATGG870.14724549378014726No Hit
TTCCGAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC870.14724549378014726No Hit
ACGCTGCGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC860.14555301684014554No Hit
AGACCAGGGATGCACAAAGAGACCGATGTGAAACACAGTGTCAAATGAAGAA860.14555301684014554No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTTTCCGAAATCTGTCTCTT850.14386053990014386No Hit
TAGTGCGGGAGGGATCCTGCCCCAAATGCGGTCGGAGCGCCAGTGACCCTGA820.13878310908013877No Hit
ACTGCCGGGGTCCTGTGATTGCGGGCGTGAGCCACTGTGCCTGGCCATAAAG810.1370906321401371No Hit
GAACCGGAGCTCTCTTACAGTAAAATCAACCATTTTTTCTTTTGATATTATT810.1370906321401371No Hit
AAACCTGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC790.13370567826013371No Hit
TGTAACGGGATGATTTGGCTCTCTGTTTGTCTGTTATTGGTGTATGAGAATG790.13370567826013371No Hit
TAAAATGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC790.13370567826013371No Hit
GTGTGGGGGATATATGGAAAACATTTAATGCAAAAACAATGCCATAAAGTTG770.13032072438013032No Hit
GCCATGGGGACACCATTTTGTAAATGAAACTTAAGAATTGAATTGGAAAGAC770.13032072438013032No Hit
GACCTAGGGACCAGTCATAGCATTTACTTCTGCTTCTCCTGGATTATGTGCT760.12862824744012863No Hit
TATTCTGGGCGATCTAAATTCGGGAGGATATAATGTGTTAACCTTTTGTGAT760.12862824744012863No Hit
CAGCCTGGGTGACAGAGCTCGACTCCATCTCAAAAAACAAAATAAACAAAAA740.12524329356012526No Hit
AACGAGGGGCAATAAAATCTGGGTTGATTTATAGTCCCATAGTTAATTCTTT740.12524329356012526No Hit
GCTTTTGGGATAATTATTTTCACTTTTTAATTGCATAAACACACACACATAT730.12355081662012354No Hit
TAGTGACGGGAGGCACACTTAATACATTTTTTTTCAAGCGAAAGTGTCAGCA730.12355081662012354No Hit
TTACCGGGGACGAGAGCTTCCCACCTTCTCACCTACACCTGTGATCTGCCTC720.12185833968012184No Hit
GTATTGGGGACCCTCTACAAGGCCTACCTGGAGTCCTTCTACAAGTTCTGCA720.12185833968012184No Hit
TTTACTAGGGAGCTGATGCCTGAATGTATCCTAGCTGACAAATTATTGATTA710.12016586274012017No Hit
TTCGCTTGGGATCAATGTTCAGTTCAAAAGCCTGAAAGTTTAGATCTAGAAG710.12016586274012017No Hit
TGAGTAGGGAGGGCTCCTCCCTCCTACCTCAGGCCTGATCCATCGTGCCCTT710.12016586274012017No Hit
CATTATGGGAAGGACTCCTGAGGCCACCCTGGGCCAGCCCTGTCCATACTAC710.12016586274012017No Hit
AAGTTTGGGAGGCTCAATTTTGCTGTGAACATAAACTTCTCTAAAAAGTAGC710.12016586274012017No Hit
GGAAATGGGAATGTGGCACTTTATGTCTCCATTTCTTCCATTCTTTTCCTTT700.11847338580011847No Hit
ACAATGGGGAGAAGGTCACAACACAGGCCTTTTTCACTCAGCTTTGTAGGCA700.11847338580011847No Hit
GACTACGGGTGATCTCGGTTTGGGAGACTTAGGGTTTATCTATTTTTTTTTT700.11847338580011847No Hit
TGTGCCGGGGCATACACATACATGCACACACACACGTTATCCCATGATGTTC690.11678090886011677No Hit
ATCTTAGGGCCAGCAGTGATGTCAAGTGCCCAGTGCCTTGGGGAACCGGCTT690.11678090886011677No Hit
GTAGAGGGGATATCATACAATGGAATACTATTCAGCAACAAAAGGGAATAAA690.11678090886011677No Hit
GCTCTAGGGATTTCAGTACTTAGCAATTAGAGTTCTTTTATTAAGATGTATC680.11508843192011507No Hit
TTAGTGGGATTGCTGTCATGGCGTCAGTTCACCAGACTCATTGATTTTGTTT680.11508843192011507No Hit
TGTACAGGGATTGATGGAGGTGAAGGGCGGAAGAAGAGTGGGCAGACCCGGT680.11508843192011507No Hit
TACTGCGGGAAGACTTAAAGCAGTCTTGTTAAATTGACATAAATGGTAAACT680.11508843192011507No Hit
TCGCGTGGGCGATCTACGTGCGGATTAAAATATGTTCATATGAGTCATCTGA670.1133959549801134No Hit
CGTGGCGGGGAGAAAGTTACAGTGCCCACAAATCTATTGCTATTAGAATATT660.1117034780401117No Hit
CGGCGCGGGTACCAGCCAAGATAGATAAATATGCCCAATAAGTATAATTGTT660.1117034780401117No Hit
CAATTAGGGTAACTTACACCTTTAAAGAGAGACACATAGAGCAGGAATTTTT660.1117034780401117No Hit
TCATTAGGGATCTGACTTTTCCACCATTTCTATCTGTATCAATTGTATTAAT660.1117034780401117No Hit
GTTCTTGGGCAATTCATATGGAATCTAGATGGATATTTCATGCATACCCATA650.11001100110011No Hit
GTGGATGGGATCCAAATATTTGCATTGCATACTATTTGTTAGCAGACAATAT650.11001100110011No Hit
CTCACTGGGCTTTCCCTTTTTGCCATTATTCTCAGCTCCATTCCCCAAGACA650.11001100110011No Hit
ACACGGGAATTTTCTGTAACTAAGAATAATTGAATAGATATTGGGTAATCAA650.11001100110011No Hit
GAAAGTGGGAGGGAAGTTATGAATTACTCCTTCCAGTAGTAGCTCTGAAGTG650.11001100110011No Hit
TAGTACGGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTAC650.11001100110011No Hit
GAATGTGGGAGCCTTTTTCTTGTTTGGCTTTTGTGCCATTTGCCCCTCACTG650.11001100110011No Hit
CATCCTGGGGATGGATTTAATTCTTGATGCTGGTGCTAGAAGTAGGTCTTCA650.11001100110011No Hit
GTGAAGGGATAAAAGGATAGCACAAGAGATCCTTGTGGTGATGGACATATTC640.10831852416010833No Hit
GGCCGGGATAGTATAGTCCAACATTCGTTGATCCCAATACAGGCAAACAACC640.10831852416010833No Hit
CATAGAGGGAGAAACCTTGATTAGAAGTGGCGAAAGAAGGAAAGAAACTGTG640.10831852416010833No Hit
CCATCAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC640.10831852416010833No Hit
CTGCAGGGGAGTGTGTTAGTGTAGGTTTAGGCAGAAACCAGCCTGCTGCTGC630.10662604722010663No Hit
ACCATGGGGAACACCTTGATTTTAGCCCATCAAAACCCATTTTGGATGTCTC630.10662604722010663No Hit
TGTCCAGGGAGGCAAACTGGGAGGACCTGGTCACGCTAACAAATGGGAAAGT630.10662604722010663No Hit
ATCTATGGGACATTCTCTAAGCTCCAAATCAGATGTCGAGGGTAGGAGCACT630.10662604722010663No Hit
AAAACGTGGGGGGTTCTCTAGAAACAAGAGCCTGACTGGAAATTAAGTGAAG620.10493357028010493No Hit
CTTATACACATCTCCGAGCCCACGAGACTTTCCGAAACTGTCTCTTATACAC620.10493357028010493No Hit
TCATTTGGGGGAGAAAGATTGGTGGACAGGAGCAGCGGCCGGTGGGGAGGGC610.10324109334010323No Hit
AAGTACGGGGGTCTCTTATGCAAGAGCATGGGTCCCAAAAAGGTGGGGATGC610.10324109334010323No Hit
ATGCTGGGGCCAGCAAAACTCACACTTGACAAACACAGACTGCTGAATGCAA610.10324109334010323No Hit
GATGCGGGATCAAAGTTTTCATAAAACTAGCTACAAAATCCACTTGGATTAT610.10324109334010323No Hit
GTTTAAGGGGGGAAGACTAGGAAGGACTCTGAATTTCAAAAGAGGTGATTTC610.10324109334010323No Hit
TTGTAGGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC610.10324109334010323No Hit
CTCTATGGGCAGCTTGCGGGCATAATAAGCAACCTTTCTTCTCTCTGTTAAT600.10154861640010156No Hit
ATTGAAGGGCGCTCTTCCGATCTGAAACGGGGGTTCATAATATATTTTTTTT600.10154861640010156No Hit
TCGCGTGGGAGTTGAATGTTATTCAAGCCAGAAGTTTGGCAAAACACTTTGG600.10154861640010156No Hit
TCAAAGGGGGAAATAAAAAAGTACAATATGTAGTATATTAGAAAGCAATGAG600.10154861640010156No Hit

[WARN]Adapter Content

Adapter graph