FastQCFastQC Report
Sat 31 Dec 2022
SRR5847008.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5847008.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences185155
Sequences flagged as poor quality0
Sequence length52
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTTCCGAAATCTCGTATGCCGTCT6690.36131889497988173No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTTTCCGAAATCTCGTATGC5420.29272771461748265No Hit
TAACGGGGTCACCCAGACATCCTACTCATTATTCTCTTTTATCCATTTCAAA2880.1555453538926845No Hit
AAGACAGGGACAAATCTCCTTAATTATAACATTCACAGCCACAGAACTAATC2610.14096297696524535No Hit
ATTTTAGGGAATTTCTTGTATTTCTATATACAAATAACAACCTAGATGAAAA2510.13556209662174934No Hit
TCTGTGGGGACTTTCAGCAAAGAAATTTATTTTGAGAATATGAATATTTCAG2490.13448192055305014No Hit
CTTTGCGGGGGGAAAGAGAGTGGCAGAAAAACTTTGAGGAAAGTCGAGATGG2480.13394183251870057No Hit
TGTACTGGGGTGAACTGTCAAAGAACTCTGATATCGAACAGTCTTCAGATAG2460.13286165645000134No Hit
TACGACGGGGACTGCATAATTTTCTTGAAGACGTGTAACAGATCTGTGGCTG2420.13070130431260296No Hit
TCTACTGGGCATTTTTTAAGGTTGGGTCTTTCAGGCTGGTTTTGGTTTGTAT2420.13070130431260296No Hit
TTAATTGGGATAGGGACTTGTCTTTTTTTCTTTTTACATACAGCTTTTCCTA2390.12908104020955416No Hit
TGCAATGGGATAGGAATTTTGTTTTTGGTTTCTTTATCATGCTACAGAGAGT2280.12314007183170857No Hit
TTATAAGGGGATCTCGATATTTCAGATGGGCTTTTGATGCACTGTTGCCAAG2270.12259998379735898No Hit
CGTAGTGGGGGTTGGTTAAGGAGTTGTTTAGAAAGGCAAAGATGTTTAGAGT2260.12205989576300938No Hit
TCAGTTGGGCGATCTCGAAATGGGAATCTGAATAGTTATTTTACTTTTCATG2210.11935945559126138No Hit
CTTTTGAGGGGGTTAAATGTTTACTCAAATGTAGTAATAGTATGAGGCCAAA2190.11827927952256218No Hit
TTCATAGGGTATTTTAGCAGTTGATGTATTTTGCCTTCACAGTACATTTCTA2170.11719910345386299No Hit
AAATACGGGACATCCATCCAGCGGAATACCATTTCCCTCAAACCAGCAAATA2150.11611892738516377No Hit
ATTAACGGGATTTCAGGTGGATTCTTTTGTATGATGACTTATAAAAGCTGAT2130.11503875131646459No Hit
GATTACGGGGTCAGGGAGAACCTCAAAGTGTGGATCTATCCCGAGGGTACTC2130.11503875131646459No Hit
TCAGTAGGGGATGTGGGAGTTTAGTGCTATAAACTTTCCTCTTAACACTGCT2100.1134184872134158No Hit
TATCACGGGATGAATGCATATCCTATTTCCAGGCAGGCTCTCTTACTTGAAC2100.1134184872134158No Hit
TAGGTGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA2100.1134184872134158No Hit
TAGACACGGGACTCAACCTGTATTTTTCTTTTATACTTGAATTCTTCAAAAT2100.1134184872134158No Hit
TTGTAAGGGTGAAGTAGGCATATGAAAGAAAAGGCACTTCTTGGACAAGCAC2090.1128783991790662No Hit
AAGTTGGGGATAACTTCTGTTATTTCAGTAATAATAATTAACCCCAAAATTC2080.11233831114471658No Hit
GTCACTGGGATAAATATAGGTATTTATCCCTCTGCTGGATTCATAAGCATCG2080.11233831114471658No Hit
GTCTTTGGGAGCCTAGTCCTCTCCTCCTCACCACCTCCTCAGCCACCAGCCT2080.11233831114471658No Hit
TATTATGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA2070.11179822311036698No Hit
AGCGTGGGGACGCTAACGTAGATAAACACCTTAATAATCTCAGTTAATACTG2070.11179822311036698No Hit
CAGCTCTCCCACCACTTTTCTCTATTGCATTTGCTCTCAAACTAGGGAGATT2070.11179822311036698No Hit
AATCTAGGGATCTCAAAAAAAGAAAGAGGAAAAGATATACATAGAAACAACA2070.11179822311036698No Hit
CGTTCTGGGAGAACCTGTGATGTGGGCAATGGGGAGAATGAATGTGACTTTT2060.11125813507601738No Hit
CTCACTGGGGTTAGCTTCATTCTATAACAGATTTTCTCAGTGTGGTGGAGAA2050.1107180470416678No Hit
GAGTTGGGGGTTACACATAATGAATGCATCTAAGTAAATTCCTACAGGGTCT2050.1107180470416678No Hit
ATGTCGGGATTGTTACAGAAATGGCATTGGAACCAACATGGCACTGTGAAAA2030.10963787097296859No Hit
GGACTGCGGGAGAAGGGAAGGAAAAATGTAAGGTTTAACAGTAAGCACTTGC2010.1085576949042694No Hit
AAGTTGGGGATCATTTTGTTGCGCTTCTGAATTCTTGTTACCCATATCACTA2000.1080176068699198No Hit
CTTTAGCGGGCGGCGGCGCACCTCGTGGCGAGAATCGGGAGAAGGAGGAGAC1990.10747751883557019No Hit
GAATGTGGGATTTTCTTAATGAAACAAAAGGGAAAATCAGCCCAATAATAAT1990.10747751883557019No Hit
TTGCCTGGGCTGAACTAAAATACATATTATACTTTATTGATCATTGACGTTA1980.10693743080122059No Hit
CACAGCGGGGCAATCAGCTAGAGCAATGTAGGCTTTTTTTAATTTAAATTAT1980.10693743080122059No Hit
TTACACGGGGTTTTATGTTACAAAACAGGTTATTTCACTATATGTATGTTTA1980.10693743080122059No Hit
AGTTTAGGGGAGTCTATAGATCAGAATGAATGGGGCCTAAAGTTTTAAAAAC1980.10693743080122059No Hit
TGCTTTGGGCCTTGATGATTTTTTTTATCACTGGTTACTATCTTCTTAAAAA1970.106397342766871No Hit
CTTCATGGGATTGCTTACACTTTTGAGGTCTTAGTCATAAATAATTTGCCTA1950.1053171666981718No Hit
AGATCAGGGGGTCTTACAGCTGATCCGGTTCTTGCGGCGGTGCGTGCTGGCG1940.1047770786638222No Hit
TACACGGGGCGATCTACGATGGGGATTGTATGTTACATATATTATGTACTGT1940.1047770786638222No Hit
TGCACAAGGGCCCCAAATTTAATATATCCAGAAACAGACTTCCTTTGCCATC1940.1047770786638222No Hit
TTCTATGGGACCTCCTAATTTTGCCCTTCAAAATTGCCAAGAGGTTATTTCT1940.1047770786638222No Hit
ATTGCCGGGGAAAACAGAATCAACAGAATGTATTCCTGAACAAGACCTGAGG1930.10423699062947261No Hit
CAGTATGGGCAGAGATACACATGATCATGTTACAATTTGAAGTGTTTTAATG1930.10423699062947261No Hit
TTATATGGGGAAAAACAAAGAAATTAACACATTTAAAAAATGAATGATAGCA1920.10369690259512301No Hit
ACTGGTGATCCCTGGTTTGGCTTGGCTGGGCTTGAGCAAATGAAAAGGAACA1920.10369690259512301No Hit
CACCATGGGAGTTTAACTAATGTGGGGCTGGCCACGTGGAAGACAGTTATTA1920.10369690259512301No Hit
ATGCATGGGGTAAAGACATAGGGATAGGTGGGAGAGGTCCATTCTTTCATAA1920.10369690259512301No Hit
ATTAAGGGAGAAAATCAAATTAGTAGAACAGAGGACAAACTTGAGAAGCAGT1910.1031568145607734No Hit
CAGCTAGGGCCCAGCAGTTGGTTTGAGCAAGCCCTCAAGTGATTCTGATTCC1910.1031568145607734No Hit
GACAATGGGATAGTACACTACATGTATCTGTATTATAAATAGGCTTTTAAGG1900.1026167265264238No Hit
CTCCAGGGGACAGTCACCTAAGTCACTGAACACAAATGTGATCAGATTATTC1900.1026167265264238No Hit
CATTCCGGGGAATTGTCCTATAATAAATCACTTATTTACAACCAATCAAACT1900.1026167265264238No Hit
TTGCACGGGATGGAATAAACCAGTAACTAATATTTCAGCTGTTGAAACAGAA1890.1020766384920742No Hit
TGCGCAGGGATGGTAATGTCCAGTTTTAAAAATATTGGACATCTTCAATCTT1890.1020766384920742No Hit
ATTGCTGGGGGGTAAGGTTAGGTTACTGATGTGAGATTTTTTTCTTCCTTTT1890.1020766384920742No Hit
TAAAGTGGGATTCTAAATAAATACATATTTGTACCTAAATATGCATAAATGA1870.10099646242337501No Hit
AAATGCGGGGAAAAAACCACAAGCTCACTTGCCTGTGCATACAGAAAAACAG1870.10099646242337501No Hit
GTTTATGGGAAGGACACAAGAAACATGAAAAGGAAACATACCTCTAATGGAA1860.10045637438902541No Hit
TGCGTTAGGGAAATCAAGTTAGATTTATAAAAGGGGTTAATTTTTGGCCTAC1860.10045637438902541No Hit
TTGCTAGGGTGATTGAATAAATAAATATAAACATATGGAGAAAGAGACAAAT1860.10045637438902541No Hit
ATACAAGGGATAATTTATTATGGTATTAAGTTCAATAGTGATTGGTTAAATA1860.10045637438902541No Hit
TCTTAAGGGGAAATCCTAAATGATATATGGCATCATAAATAAAATATTATAC1860.10045637438902541No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)