FastQCFastQC Report
Sat 31 Dec 2022
SRR5847000.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5847000.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences111757
Sequences flagged as poor quality0
Sequence length52
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTTCCGAAATCTCGTATGCCGTCT7310.6540977298961139No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTTTCCGAAATCTCGTATGC6190.5538802938518393No Hit
ACTCTCGGGCAGAGGGTGTATTCAGGCAACTGGTAACTATGCCACCATTTCA2180.1950660808718917No Hit
GTTGATGGGTATGGAGGAAGGTTTTGCTCATGCATGGCATGTTAGTCAATTT1930.1726961174691518No Hit
AGACCGGGGATGGGGCTACAGGAAGACAGCAAGGGAATCCAGACTGAGCAAA1870.16732732625249425No Hit
GAGTCAGGGGATCTCGTGTTGGGAGTCACTCTGTTGACAGTATCAATGGGAA1850.16553772918027507No Hit
TGTGCAGGGTGGCTATTCTAGTTCCTTTCCATATAAATTTTGGAATAAACTT1720.15390534821085033No Hit
TCTCTGGGATTCACAGTTGCTTCAAAGAGAATAAAATACCTAGGAATCCAAC1660.14853655699419277No Hit
CTATCACGGGGAACTGCAAAATATACATTCTATTCAACAGTGCATGGAACTT1650.14764175845808317No Hit
GAAGGTGGGGTTACTTGTAATTGAAAGTATTCTCAACAAATATAACCAAACT1580.141378168705316No Hit
CCAGCGGGAAGTTCTCTGAGGATGAGCACTAAGTAGCTGACATTTCTAAGTT1580.141378168705316No Hit
CATACTGGGATAGTAACTACTGAAGAGATAAATTGTAAAATGGAAACTATAA1550.13869377309698722No Hit
TGTTTGGGACCATATACCATAATAAACTCTGAAGGCACGAGAAATCTAAATG1540.1377989745608776No Hit
TAGGCAAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGG1540.1377989745608776No Hit
GTCACTGGGATACTGGCTTTGCTGTAATGCTTGAAAAGGCGTTGGTATCTTC1530.136904176024768No Hit
GTTAAGGGTCTCTTAGCGGGGTCACTGAGAAGTCAAGTAACTTCCTCTAAGG1530.136904176024768No Hit
ATTTCCGGGGCTTCTTTTCCGACAAAACACCAAATGGCGGATGACGCCGGTG1520.13600937748865843No Hit
CTGAGGGGGTGATACCACTTTCTTGAAATTAAGTCCCTCCTTAGTTTTTGCC1520.13600937748865843No Hit
AACATGGGAGAAAAAATAATTTAAGAAAAACACTAAGCCATAGATATACTCT1510.13511457895254883No Hit
CTTATTGGGGCCTACTTGGATGATTGTCACCGGAGGTATAGCTGTGCCCACT1510.13511457895254883No Hit
CATCTCGGGAGCCCAGTATATCAATGAATATAAAAATTTGAGAAGATATTTT1500.13421978041643925No Hit
TGCCGCGGGATCTAGTGACTTTTTAAAGCCCTAACGTTTACATAAAGAAGCT1490.13332498188032965No Hit
AGACTGGGGATGGTATACATCACTTTTGACCTTGTCTAAATATCTCAGGAGG1490.13332498188032965No Hit
TTCTTAGGGACCTAAACACTTTATCTCCAAGTTACAAAAGTTTGAGGTGCAG1480.13243018334422005No Hit
TATGCTGGGAGGCAGAGTGTGGTTCAAATACGCCAGACACCTTTTGAAAGCT1470.13153538480811044No Hit
CTCTGTGGGGCTCTTCCGATCTGTATGAGGGCAAGTATTTTCTTAAAGATAT1470.13153538480811044No Hit
AAAGCGGGAGTTAAAATTAAAAGCAAAAGATTAGAAAAATTAAAACATTACA1460.13064058627200087No Hit
GCATACGGGAGGAGAGGCAAGAGAATCACTTGAACCCAGCAGGCAGAGGTTG1460.13064058627200087No Hit
ATATAAAGGGATCCCCATCAAGCTACCAATGACTTTCTTCACAGAATTGGAA1440.12885098919978166No Hit
TACAAGGGGAACCACCAATGCGCTGGACTTGAATTCAGTGCTTGGGAAGGAT1440.12885098919978166No Hit
CTTCGTGGGAACCTTGACCTTTTTTCCCTCTGGTTTGAGAAAACTTTGGACA1440.12885098919978166No Hit
ATTACTGGGCCACTATGCACATTTTATTTCTCCAACATACTCGGATTCTACC1430.1279561906636721No Hit
AAACACGGGGACATATAAATGGCTAATAAGTATATGAAAAAATGCTCAACAT1420.12706139212756248No Hit
AGACTAGGGAGAAGCAAAGCTCAGAGAAGATGCATTAGGATCAGATTATAGG1410.12616659359145288No Hit
AGTTTTGGGATAGTTCTATGCTTACTGTTGTGCATATACTGGCAATAAAACT1410.12616659359145288No Hit
TACTAGGGGGATCTAGTGTTTGCTGTCATTAGTGACCAAGAAAAAGTAGTTC1390.12437699651923369No Hit
AGTCGAGGGTGGCGGGGGACAGTAAACTTTAGACAATGCTACTTAATCATTT1390.12437699651923369No Hit
GCGGCACGGGATTATTTAAATTGTTCATCACAGTAGAAGTCATTATTTTTAA1380.1234821979831241No Hit
ATGTCAGGGATTTGAGGGCTTAATTGTGTATAGTAGAAATACTATTTTAGAC1380.1234821979831241No Hit
ATAAGAGGGAATGTAAGACACGAAATTGTTAAACTGCTAGAAGAAAACATAA1380.1234821979831241No Hit
ACATGTGGGCGGATTCTTGAGTGGATTAAATGAGTTAGTTTTGGTAAAGTTT1370.12258739944701451No Hit
TTTTGCGGGAAAAGAGGCATTAATCAGGATTGTAGTTCATCTCTCTTTTTAT1370.12258739944701451No Hit
TCAGCAGGGAGAGTTTCTCAACCTTCAGATATTGAAATGAAGTGTTGATGAA1370.12258739944701451No Hit
AACCATGGAGGAAAACTCCAGTGACTATCAGTCAAACCTTGCTTAGGTAGAG1370.12258739944701451No Hit
CTTATACACATCTCCGAGCCCACGAGACTTTCCGAAATCTGTCTCTTATACA1370.12258739944701451No Hit
GCATGGGGTGGCAGCAATTTTGTGTTTTTATTTTGGAGACGGCTAATGGTAG1370.12258739944701451No Hit
TGTTAAGGGAGGGCCCATTCAGTGTGTTTATGTTTTCTAAGGCTTAACCAAT1370.12258739944701451No Hit
TGTACTGGGGACAACATGAAAACTCCATATTTATTTAGATGCTATTATTACT1360.12169260091090492No Hit
CATCTAAGGACGTATCCTATATTTTGAATTTTATACTAGTTTTTCAAAAGTA1360.12169260091090492No Hit
CGTAGCGGGGATCTGGTTCTGGGATTGAATACATTAGCCATTGATAATCTAC1360.12169260091090492No Hit
CTCCAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1350.12079780237479532No Hit
AGTACTGGGATTATTGAAGCTTTGTAATATAATTTGAAATCAGAGAGAATAA1340.11990300383868573No Hit
ATCTCCGGGACCCCTGGTCAATCAATGGCCCTCACTGGGAACGCTTAATGGG1340.11990300383868573No Hit
ATTACGGGGATTCCTCTCTGGGTTTCAAGTCGAGAGTATTTAAATTATAAAA1340.11990300383868573No Hit
GGGTAAGGGTCTCTAAAGGGGAAATCAGAGATAGTCCTGTAAGAAAACTTCC1330.11900820530257611No Hit
TTGGACGGGACAACATACCAGAATCTCTGGGACACATACAAAGCAGTGTGTA1330.11900820530257611No Hit
CTATCCGGGGACGCTCTTCCGATCTTTTACGGGGATGGATTTTAATTGTGTT1330.11900820530257611No Hit
AACAAAGGGAACTTCTACCTCCCAGGTTCAAGCAGTTCTCCCTACCTCAGCC1320.11811340676646652No Hit
TTTGCAGGGAACTTCCTACTGGGAATGACCCGTTCCTGCAGGGTCTCACCTT1320.11811340676646652No Hit
GTAACGGGGCAGGATTTATCTGCTTTGATGCTGCAACAAAGGCTGTCCTTTG1320.11811340676646652No Hit
ATTCCAGGGATACACCATAAATTAATCCCATTCATTCTAAAAACTTGTTCAT1310.11721860823035693No Hit
GCGCTAGGGGTTTGGGCAGTTTGGCAAAATCCTAGATGTAGAAATAATCTTT1310.11721860823035693No Hit
TACTCTGGGATTGTTCATGAAAATGGAAGAACAATGTTTATCACAAAACTAG1310.11721860823035693No Hit
AAGCCAGGGATGTGCCTGATGTGGTGGAGGGAGACACCAGCAGGTGGCTGCA1300.11632380969424734No Hit
GCTTTGGGATAAATCACTGATTTTATTTTCTACAGGTTATTTAGATGTTTGG1300.11632380969424734No Hit
TTAGTTGGGGGAGTCAAGACCTGGTTTCTAGTCTTGGTTTTGCCATTAATGT1300.11632380969424734No Hit
GCGCCATGGGTCTCTAATTTTCCTGTCAGTTATATTTTGGTGGAGTCTGGGT1300.11632380969424734No Hit
AAAGAGGGGAAGATGTATATTATAACCATAGAATAATCACTAAAAAGTACAG1300.11632380969424734No Hit
GCGCTGGGGACTGCTCGAGACACAAGTGCTGCTGTAGTTAGCGCTTAGTGAC1290.11542901115813775No Hit
CCAATGGGGGGCAGGTCAAATTCTGAGAGCTCTGGCTGTGGTACATTTCTTC1290.11542901115813775No Hit
TGTCAAGGGATCAGGAAATCCCAAAGTTTCCAAAGCTTTTTGTTTACAGAAT1290.11542901115813775No Hit
ACAACTGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1280.11453421262202816No Hit
CGTGCGGGGGGCCTCTAGAACTGTGAAAGAATAAACTTCGGTATTTGTAAGT1270.11363941408591856No Hit
CACAAGCGGGATCACTCTTATGTAATCCCCAAAGGCTTGGCATGCCGTAAGT1270.11363941408591856No Hit
AGTAACGGGGATGATAGGAGTTGGCAGTTTTTTAATGCTTAAAGAGGAATTA1270.11363941408591856No Hit
TTCTTAGGGACCACCTCCTTACTTACCTGCCTGAAGAAATTCTGCCTTTGAC1260.11274461554980897No Hit
ATGCCAGGTGAAATGAGATGAGTTGAAATGATGAGATGTAATGATGAAATGA1260.11274461554980897No Hit
CTAGAGGGGTCATTATCTTCATGGCTTTATTATCTACAATAACTTTTAAATT1250.11184981701369935No Hit
AACCAAAGGGGAAACATTTTGTAGTAATAATAATCAATTTTCAGCTGATAAA1250.11184981701369935No Hit
GCGTGAGGGGGCAGGTCCCTAGCCTGCAACATTTATGTCGCATGTCAATCCG1250.11184981701369935No Hit
CTGAAAGGGATTTTCAGAAGTTTCGGCTGATGGAGAAAAGAGAGTCCTTAAT1240.11095501847758976No Hit
TTTGTGGGAGTGACACAACTTACCAAAACCTCTGGGATACAACAAAAGTGGT1240.11095501847758976No Hit
ATAGTTGGGGAGAAGGGAAACGTGAAGAAGGTGAAGATGGCGGTGGCCAGGG1240.11095501847758976No Hit
GAAACAGGGATACTTGGTTGATTTAGGTTGCACATTCACTGAATTCACTGAG1240.11095501847758976No Hit
AAGCGAGGGGACAAGACTCGGCTTTGACCACAGCCCTTTCTTGCTCCATTCC1230.11006021994148017No Hit
CCATGTGGGGATTACAAATGTGGAAAATGTTATAATGTCATATTCTAGCATT1230.11006021994148017No Hit
GTGGTAGGGATTGGCCAGGCACCAGAGTCCATTTCAGAGAAAGAATTAAAAT1230.11006021994148017No Hit
CCAGACAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1230.11006021994148017No Hit
TTTGTAGGGGATTTTCTAATTATCCCAAATGATTTTATAAATTCCGTAACTT1230.11006021994148017No Hit
TCATATGGGTGTAAAAACAGTATCCCATATTGCATTGCAACAGGTCGACACA1220.10916542140537058No Hit
GGTATTGGGAACTGAGCTGCTGCCTATAGTGTCTTTTGGAAAACTACTTAAA1220.10916542140537058No Hit
TACGCTGGGGGAATAATTTTCAGTGCACATAAAGCTTGAACTTAAGTAACAT1210.108270622869261No Hit
GCATTTGGGATTTGTTAACCTTGTTCTTTATTCCTATTTTTGTCTTCCATTC1210.108270622869261No Hit
TAAGAAGGGAGTTGAATGAATGAATAAATAGTGAAGGTGCTTAAGTGAGGGA1210.108270622869261No Hit
TTGGTGGGGAGCTGAGTTCTGCTTATGGGGAGTTTTGGTGGGAGATTGGAGG1210.108270622869261No Hit
GCAATGGGACTTGGATACCACCCAGCAGATGATTGCTAATTCCTTGAAGGAC1210.108270622869261No Hit
CTGCTTGAGGGAAGTAATCTGACACTAGGTGCCACATAATTAAGATAAAGAC1200.1073758243331514No Hit
TTATAAGGGATAATCAGAAAGTGGTAGAGTCAGTACTTAACTCTGGGTGTCT1190.1064810257970418No Hit
TTTCGCGGGATCTCTTAAACAGCTTGATTTTAGCATCATGACTTTCCTCTGG1190.1064810257970418No Hit
CTCTCCGGGTGGTATATATATCTTGTCTGTGCTATGTACATGTACTTTCTGG1190.1064810257970418No Hit
TATAGTGGGATCTACAGCCGGGACCTCGATGTCGGCTCGTCGCATCCTGGGG1180.1055862272609322No Hit
TGTTTTGGGAGCAAACATTAAACAAAGCCCAATTCCTCATCAGATTTCTGTA1180.1055862272609322No Hit
CACGAAGGGAAGTCACGGTATTATCAACACACTTATTCACAACCCAAAATAT1180.1055862272609322No Hit
GTAACTGGGAGGGGAGCCAACGTTCCTTGTAAAGTGTGTAGATTTTTTGTTT1180.1055862272609322No Hit
AGGATGGGGAGAGACCTTAAAGCCAGGAGACTTGATGATCTACCATGCTTTC1180.1055862272609322No Hit
CAGATGGGGGACAACACATACACACTTGCCTCTCCCCATCTCCAGAAGAGGG1180.1055862272609322No Hit
GGTAAGGGAAGTATAGACACTTGCATTCTTATTGTATTCAGTGGATTATGAT1180.1055862272609322No Hit
GGGCCAGGGCACATTTTCTTTACTTTTTAGTCTGTTGATAGACATTTAGGTC1170.1046914287248226No Hit
TACTTAGGGGACTGTTTTGATTTAATATTAGTAGGAGCTCTTCATGAGAAGA1170.1046914287248226No Hit
TACCTAGGGAAGTCTTGCTTCTGTTTATTTCCAGACACTGTACTCATGAGCC1170.1046914287248226No Hit
CTTCGGCGGGATCTTGACAGAGCAAAGATTTTAATTTTATAAATTATGATTT1170.1046914287248226No Hit
AGCTAGGGATGCCTCTTTTTGTCTCATTTTTGATGCTATATTTTAAATTTTA1160.103796630188713No Hit
TTGAACGGGCTTGAAAGGGGACCATGCTTCTGGCATGATAAAATCATGGAAT1150.10290183165260341No Hit
TATGCAAGGGATCATTTTTTGAGGACTGTCTAGAAAAGGTTGCCTAACCCTC1150.10290183165260341No Hit
GTCACAGGGATTTAATTAAATTTTAGTAAATCTTATTGAGTAATACAGTTGT1150.10290183165260341No Hit
GCTCTTGGGGGTCCTCAGCAAGAGAGACATCACAATCTCACAGGTGATCCAC1150.10290183165260341No Hit
TTTGTAGGGATGGAAGAGAACAGATAGCTCTGTAAATAGAGCCAGATGTCTC1140.10200703311649383No Hit
TTAATTGGGGGATTCCTTCAGGTCTTTCCTTTGGTTCTTTGAGCATCTTTGC1140.10200703311649383No Hit
ATCAAGGGATCTGCCTTTGGGAGATGAAATAAAGACCAGAGAGAGAATAAAG1140.10200703311649383No Hit
TGGCGCGGGTGGGGCTGTGAGGACTAAATGAGTTAAGACATGAAAAACCCTG1140.10200703311649383No Hit
GAAACTGGGATGCATTGTGTATTAAGAGAGTTTATATTTTAGTAAAATTTAA1140.10200703311649383No Hit
CATCTAGGGAAGCATAGTTACCACATGACCCAGAAATTCTATTCCTGAATAA1130.10111223458038424No Hit
GTTGGGGGTTGAATGAATGATCCTTCATAATTACAATTTTTAATGCCTTTAC1130.10111223458038424No Hit
ATTGTAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1130.10111223458038424No Hit
AGCACCGGGAGTAAATTCAAGAGTAGAGAGGAAGGCTTTCTAGGGTCCTCAC1130.10111223458038424No Hit
GGTCGGGAGAAGCAATGGACGAACCAGTCCTCACATCTTCTCCCGCCATAGT1120.10021743604427463No Hit
CACAATGGGATGCTATAAAAATATTTCTAGGTAATGGAATTTAGTAAGAATT1120.10021743604427463No Hit
ACACCTGGGACCATGGAATGAAAAATATTGTTATACAATACATTGATCTGTC1120.10021743604427463No Hit

[WARN]Adapter Content

Adapter graph