FastQCFastQC Report
Sat 31 Dec 2022
SRR5846999.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5846999.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences111893
Sequences flagged as poor quality0
Sequence length52
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTTCCGAAATCTCGTATGCCGTCT6740.6023611843457589No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTTTCCGAAATCTCGTATGC6270.5603567694136362No Hit
ACTCTCGGGCAGAGGGTGTATTCAGGCAACTGGTAACTATGCCACCATTTCA2160.19304156649656368No Hit
GAAGGTGGGGTTACTTGTAATTGAAAGTATTCTCAACAAATATAACCAAACT1800.16086797208046974No Hit
TGTTTGGGACCATATACCATAATAAACTCTGAAGGCACGAGAAATCTAAATG1750.15639941730045667No Hit
AAACACGGGGACATATAAATGGCTAATAAGTATATGAAAAAATGCTCAACAT1750.15639941730045667No Hit
AAAGCGGGAGTTAAAATTAAAAGCAAAAGATTAGAAAAATTAAAACATTACA1740.15550570634445407No Hit
GTTGATGGGTATGGAGGAAGGTTTTGCTCATGCATGGCATGTTAGTCAATTT1720.15371828443244887No Hit
CTATCACGGGGAACTGCAAAATATACATTCTATTCAACAGTGCATGGAACTT1710.15282457347644626No Hit
GAGTCAGGGGATCTCGTGTTGGGAGTCACTCTGTTGACAGTATCAATGGGAA1680.15014344060843843No Hit
GCATACGGGAGGAGAGGCAAGAGAATCACTTGAACCCAGCAGGCAGAGGTTG1680.15014344060843843No Hit
TACTAGGGGGATCTAGTGTTTGCTGTCATTAGTGACCAAGAAAAAGTAGTTC1640.14656859678442796No Hit
GCGCCATGGGTCTCTAATTTTCCTGTCAGTTATATTTTGGTGGAGTCTGGGT1630.14567488582842536No Hit
TGTGCAGGGTGGCTATTCTAGTTCCTTTCCATATAAATTTTGGAATAAACTT1580.14120633104841232No Hit
CTGAGGGGGTGATACCACTTTCTTGAAATTAAGTCCCTCCTTAGTTTTTGCC1580.14120633104841232No Hit
ATATAAAGGGATCCCCATCAAGCTACCAATGACTTTCTTCACAGAATTGGAA1570.14031262009240972No Hit
TGCCGCGGGATCTAGTGACTTTTTAAAGCCCTAACGTTTACATAAAGAAGCT1560.1394189091364071No Hit
AACCATGGAGGAAAACTCCAGTGACTATCAGTCAAACCTTGCTTAGGTAGAG1550.13852519818040449No Hit
CATCTCGGGAGCCCAGTATATCAATGAATATAAAAATTTGAGAAGATATTTT1540.13763148722440188No Hit
TCAGCAGGGAGAGTTTCTCAACCTTCAGATATTGAAATGAAGTGTTGATGAA1490.13316293244438884No Hit
ATTTCCGGGGCTTCTTTTCCGACAAAACACCAAATGGCGGATGACGCCGGTG1490.13316293244438884No Hit
ATAAGAGGGAATGTAAGACACGAAATTGTTAAACTGCTAGAAGAAAACATAA1470.1313755105323836No Hit
ATTACTGGGCCACTATGCACATTTTATTTCTCCAACATACTCGGATTCTACC1470.1313755105323836No Hit
GGGTAAGGGTCTCTAAAGGGGAAATCAGAGATAGTCCTGTAAGAAAACTTCC1460.130481799576381No Hit
GCGGCACGGGATTATTTAAATTGTTCATCACAGTAGAAGTCATTATTTTTAA1460.130481799576381No Hit
AGACCGGGGATGGGGCTACAGGAAGACAGCAAGGGAATCCAGACTGAGCAAA1460.130481799576381No Hit
GTAACTGGGAGGGGAGCCAACGTTCCTTGTAAAGTGTGTAGATTTTTTGTTT1450.12958808862037838No Hit
GTAACGGGGCAGGATTTATCTGCTTTGATGCTGCAACAAAGGCTGTCCTTTG1440.12869437766437578No Hit
CCAGACAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1430.12780066670837317No Hit
GAAACTGGGATGCATTGTGTATTAAGAGAGTTTATATTTTAGTAAAATTTAA1420.12690695575237057No Hit
GTCACTGGGATACTGGCTTTGCTGTAATGCTTGAAAAGGCGTTGGTATCTTC1400.12511953384036534No Hit
CTTTTGACGGGGTTTTTTCAGTCACCTGAAAATAAACCACTTCATTTCAGAG1400.12511953384036534No Hit
GCGTGAGGGGGCAGGTCCCTAGCCTGCAACATTTATGTCGCATGTCAATCCG1390.12422582288436274No Hit
AGCTAGGGATGCCTCTTTTTGTCTCATTTTTGATGCTATATTTTAAATTTTA1390.12422582288436274No Hit
ATTCCAAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1370.12243840097235753No Hit
TATGCTGGGAGGCAGAGTGTGGTTCAAATACGCCAGACACCTTTTGAAAGCT1370.12243840097235753No Hit
CGTACTGGGTATTTATTCATGTACTCCCTCCTCAATACAGGACGTGGACCAC1370.12243840097235753No Hit
CGGTAGGGAGATTATTTCTGTAGTTATATTTCTCCACTCTTTCTCGAATTTT1370.12243840097235753No Hit
TTCTTAGGGACCTAAACACTTTATCTCCAAGTTACAAAAGTTTGAGGTGCAG1370.12243840097235753No Hit
GTTAAGGGTCTCTTAGCGGGGTCACTGAGAAGTCAAGTAACTTCCTCTAAGG1360.12154469001635491No Hit
CGTGCGGGGGGCCTCTAGAACTGTGAAAGAATAAACTTCGGTATTTGTAAGT1350.12065097906035231No Hit
TTAGTTGGGGGAGTCAAGACCTGGTTTCTAGTCTTGGTTTTGCCATTAATGT1350.12065097906035231No Hit
AGTTTTGGGATAGTTCTATGCTTACTGTTGTGCATATACTGGCAATAAAACT1350.12065097906035231No Hit
AGTCGAGGGTGGCGGGGGACAGTAAACTTTAGACAATGCTACTTAATCATTT1350.12065097906035231No Hit
ATGCCAGGTGAAATGAGATGAGTTGAAATGATGAGATGTAATGATGAAATGA1340.11975726810434968No Hit
TTCTTAGGGACCACCTCCTTACTTACCTGCCTGAAGAAATTCTGCCTTTGAC1330.11886355714834708No Hit
CATACTGGGATAGTAACTACTGAAGAGATAAATTGTAAAATGGAAACTATAA1330.11886355714834708No Hit
AAAGAGGGGAAGATGTATATTATAACCATAGAATAATCACTAAAAAGTACAG1330.11886355714834708No Hit
CTAGAGGGGTCATTATCTTCATGGCTTTATTATCTACAATAACTTTTAAATT1320.11796984619234446No Hit
AACATGGGAGAAAAAATAATTTAAGAAAAACACTAAGCCATAGATATACTCT1320.11796984619234446No Hit
CATCTAAGGACGTATCCTATATTTTGAATTTTATACTAGTTTTTCAAAAGTA1320.11796984619234446No Hit
ACACCTGGGACCATGGAATGAAAAATATTGTTATACAATACATTGATCTGTC1320.11796984619234446No Hit
AAGCCAGGGATGTGCCTGATGTGGTGGAGGGAGACACCAGCAGGTGGCTGCA1310.11707613523634186No Hit
TTGATTGGGAAGAAAAAGATTTAATTGTTCCATGTGTATTTTATTTATGGTA1310.11707613523634186No Hit
CTTATTGGGGCCTACTTGGATGATTGTCACCGGAGGTATAGCTGTGCCCACT1310.11707613523634186No Hit
TAGGCAAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGG1310.11707613523634186No Hit
TGTTAAGGGAGGGCCCATTCAGTGTGTTTATGTTTTCTAAGGCTTAACCAAT1310.11707613523634186No Hit
GATGAAGGGAGGAAGAATAATTTTAACAGACATGTATACTCCATAGAAACTA1310.11707613523634186No Hit
TTTTGCGGGAAAAGAGGCATTAATCAGGATTGTAGTTCATCTCTCTTTTTAT1300.11618242428033926No Hit
CTATCCGGGGACGCTCTTCCGATCTTTTACGGGGATGGATTTTAATTGTGTT1300.11618242428033926No Hit
GCGCTAGGGGTTTGGGCAGTTTGGCAAAATCCTAGATGTAGAAATAATCTTT1300.11618242428033926No Hit
AGACTGGGGATGGTATACATCACTTTTGACCTTGTCTAAATATCTCAGGAGG1300.11618242428033926No Hit
ACAACTGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1290.11528871332433664No Hit
ACGTTAGGGATTGGCTAGCTTATAGTTTGTTTTGTAGTGTTTGGAAATTAGC1290.11528871332433664No Hit
ATCTCCGGGACCCCTGGTCAATCAATGGCCCTCACTGGGAACGCTTAATGGG1290.11528871332433664No Hit
ATTACGGGGATTCCTCTCTGGGTTTCAAGTCGAGAGTATTTAAATTATAAAA1290.11528871332433664No Hit
TTTGCAGGGAACTTCCTACTGGGAATGACCCGTTCCTGCAGGGTCTCACCTT1290.11528871332433664No Hit
CTTATACACATCTCCGAGCCCACGAGACTTTCCGAAATCTGTCTCTTATACA1280.11439500236833404No Hit
GTGAGTGGGATCTAGTGATGGGGTGTGGAGTCACAATTACTGACCCCAAGTC1280.11439500236833404No Hit
GCGATAGGGACCCCCTCCAAAAGAAGCACTTTACTTATGTACTCACATATTT1270.11350129141233142No Hit
TATAGTGGGATCTACAGCCGGGACCTCGATGTCGGCTCGTCGCATCCTGGGG1270.11350129141233142No Hit
TTTAGTGGGATTACTGATTTCCAATAATATGTTGGAACAGGCCCATGGTGAG1270.11350129141233142No Hit
AAGCGAGGGGACAAGACTCGGCTTTGACCACAGCCCTTTCTTGCTCCATTCC1260.11260758045632882No Hit
TCTCTGGGATTCACAGTTGCTTCAAAGAGAATAAAATACCTAGGAATCCAAC1260.11260758045632882No Hit
ACGATGGGGGCACTGTTGGGAACTATTAAAACAGCTAACTATAAACTAGTTA1260.11260758045632882No Hit
GCATTTGGGATTTGTTAACCTTGTTCTTTATTCCTATTTTTGTCTTCCATTC1250.11171386950032622No Hit
GTTGGGGGTTGAATGAATGATCCTTCATAATTACAATTTTTAATGCCTTTAC1250.11171386950032622No Hit
TTAATTGGGGGATTCCTTCAGGTCTTTCCTTTGGTTCTTTGAGCATCTTTGC1250.11171386950032622No Hit
CTTCGTGGGAACCTTGACCTTTTTTCCCTCTGGTTTGAGAAAACTTTGGACA1250.11171386950032622No Hit
TACTCTGGGATTGTTCATGAAAATGGAAGAACAATGTTTATCACAAAACTAG1240.1108201585443236No Hit
ACATGTGGGCGGATTCTTGAGTGGATTAAATGAGTTAGTTTTGGTAAAGTTT1230.10992644758832097No Hit
GTACGCGGGAGCATTGAAATTTAGGAAAAGAATATTAAATGCCTTTGGAAAC1230.10992644758832097No Hit
CTGAGCGGGATCAGCGAGCGATTTGGCCTACTTCCTCATTGGCTTCTATTCT1230.10992644758832097No Hit
CAGATGGGGGACAACACATACACACTTGCCTCTCCCCATCTCCAGAAGAGGG1220.10903273663231837No Hit
TCAAGTGGGTGGAGTGAATAGCATGGGGGAAGGGGATTACTAATGAGGCTGA1210.10813902567631577No Hit
AAAAATGGGATAGGTCTAGATTTGTTATCAAATCACTTCCTGGAAAACAGAC1210.10813902567631577No Hit
CTCTCCGGGTGGTATATATATCTTGTCTGTGCTATGTACATGTACTTTCTGG1210.10813902567631577No Hit
TATGCAAGGGATCATTTTTTGAGGACTGTCTAGAAAAGGTTGCCTAACCCTC1200.10724531472031315No Hit
AACAAAGGGAACTTCTACCTCCCAGGTTCAAGCAGTTCTCCCTACCTCAGCC1190.10635160376431055No Hit
GCTTTGGGATAAATCACTGATTTTATTTTCTACAGGTTATTTAGATGTTTGG1190.10635160376431055No Hit
GCGCTGGGGACTGCTCGAGACACAAGTGCTGCTGTAGTTAGCGCTTAGTGAC1190.10635160376431055No Hit
AGACTAGGGAGAAGCAAAGCTCAGAGAAGATGCATTAGGATCAGATTATAGG1190.10635160376431055No Hit
TTTACGGGAGAACAAGCCATCGTCATCGTAGGAGACGACAGCTCTGTGCCGG1190.10635160376431055No Hit
GTCATAAGGGCTCTACCTGTGAGGAGGTATGGGTTTTACTGATATGGTTCTT1180.10545789280830793No Hit
AGTACTGGGATTATTGAAGCTTTGTAATATAATTTGAAATCAGAGAGAATAA1180.10545789280830793No Hit
AGAGTGGGTACTGATTTGACAGCAGTATGTATTCAACATTTAAGACTTTCTG1180.10545789280830793No Hit
CACAATGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1180.10545789280830793No Hit
CTGCTTGAGGGAAGTAATCTGACACTAGGTGCCACATAATTAAGATAAAGAC1180.10545789280830793No Hit
AAATATGGGATTAAATATAATAAACAGTAAACGTTGAGGTTCATCAATAAAA1180.10545789280830793No Hit
TTGAACGGGCTTGAAAGGGGACCATGCTTCTGGCATGATAAAATCATGGAAT1170.10456418185230533No Hit
AACCAAAGGGGAAACATTTTGTAGTAATAATAATCAATTTTCAGCTGATAAA1170.10456418185230533No Hit
GTGGTAGGGATTGGCCAGGCACCAGAGTCCATTTCAGAGAAAGAATTAAAAT1170.10456418185230533No Hit
AGTAACGGGGATGATAGGAGTTGGCAGTTTTTTAATGCTTAAAGAGGAATTA1170.10456418185230533No Hit
CACGAGGGGAGTCCCCCACAGATACTGAGGAATGACTAAATCATCTCAGTTG1160.10367047089630273No Hit
TTACATGGGATGCAAAGAGAGGCATTGGTCCATATAGTAAAATTGTCCTTGC1160.10367047089630273No Hit
CACGAAGGGAAGTCACGGTATTATCAACACACTTATTCACAACCCAAAATAT1150.10277675994030011No Hit
TGCATGGGTGAGATGCCCGTGTCTGTGCGTGTGCGTGTGTGTTTCATACAGC1150.10277675994030011No Hit
ATTGTGGGGATCGTCACCAACGTGGCCTCCCAGTGAGGCAAGACCGAAGTAA1150.10277675994030011No Hit
TTGTGGGGGATTTCCCAATTTCAAAAGTTACTGGAAAACTACAGTGTGGTAC1140.10188304898429751No Hit
AGCTTGTGGGAGGAAGATACAATGATAAAAGATCTTCTGCATGGCACATTAT1140.10188304898429751No Hit
GTCTACGGGATTCATATAATTTTTTTATTATGTATTTCTTTTTCTGTATCTG1140.10188304898429751No Hit
TACTTAGGGGACTGTTTTGATTTAATATTAGTAGGAGCTCTTCATGAGAAGA1140.10188304898429751No Hit
CAGTATGGGAGATGGAGAGGACAGAGTCAGTCTCCTTCGGATAATTCCAATC1140.10188304898429751No Hit
TTGAAGGGGAAATCACAACAAGTGATTGTCCAGGGTGTCCATGAGCTCTGTG1140.10188304898429751No Hit
GGTATTGGGAACTGAGCTGCTGCCTATAGTGTCTTTTGGAAAACTACTTAAA1140.10188304898429751No Hit
GCTTTCGGGAAATCGCTGGATTTGGCTTTCCAGGTGACAGAAATGGGGCACT1140.10188304898429751No Hit
GCTCTTGGGGGTCCTCAGCAAGAGAGACATCACAATCTCACAGGTGATCCAC1140.10188304898429751No Hit
GCAGTGGGGGATTTGGGCAAGCCCAACTCAAATAAAAGACCTTGCATTCCTC1130.10098933802829488No Hit
CCATGTGGGGATTACAAATGTGGAAAATGTTATAATGTCATATTCTAGCATT1130.10098933802829488No Hit
TATCACGGGATGATCATCATTTCCTTGCGGACTTCTCTGCCTGGTTTTGTGT1130.10098933802829488No Hit
ATCAACGGGTGGCCATGTGCCAGGCAATCATAAGCAGCAATTCTGAACGGCT1130.10098933802829488No Hit
AACCTTGGGACATACTTTGTATTTTATATGTATTATTGACAGTATTCTTACA1120.10009562707229228No Hit
GTGCAAGGGACAGTAAATATATAAGATGAGCCTGGAACATCTTAGAGTGTCA1120.10009562707229228No Hit
TACAAGGGGAACCACCAATGCGCTGGACTTGAATTCAGTGCTTGGGAAGGAT1120.10009562707229228No Hit
GATACTGGGAACCAACAATACTTCCACTGCTAGCTTCCCCATAATTTTAGCA1120.10009562707229228No Hit
CTCTGTGGGGCTCTTCCGATCTGTATGAGGGCAAGTATTTTCTTAAAGATAT1120.10009562707229228No Hit
AATCACGGGAGGTCAATTGTATTTCCTCTTGGAAAGCAGTTTAACATGTAAG1120.10009562707229228No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)