FastQCFastQC Report
Sat 31 Dec 2022
SRR5846993.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5846993.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences153001
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTTCCGAAATCTCGTATGCCGTCT10650.6960738818700531No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTTTCCGAAATCTCGTATGC8880.5803883634747485No Hit
GAGTCATCTGACACTGATGATGAAGAATTACGGCATCGAATCCGGCAAAAAC2720.17777661583911217No Hit
CGTTCAGGGGTCGGCAGCCGCCTACACCAAGGCAACAGCGCGGAAGTGTTTG2410.15751531035744865No Hit
GATTAGGGGAACCATTTAATTCATACATGGATACCCCTGGGTTGCCTAAGAT2370.15490094835981463No Hit
AGCCATGGGGAGGAAGCTATGTTTCAACACCTGCAGTAGGTGCCTGAAATCA2120.13856118587460212No Hit
ACAACTGGGGAATAAAGGCCATTTGACTTAGATCGCCATCCAAGGCTCTCTG2090.13660041437637663No Hit
ACAGCAGGGACACGCTTATGGATGTGTGTACCATTGAGATGAGAATGGGTAG2040.13333246187933412No Hit
GTACAAGGGATATCAACACTGGAAAAGAGACTTCACTATTTTCGAAATTATC2020.1320252808805171No Hit
ACGCCACTGGGATAAGAATTCATAAATTTGAATGATGTAGAGTTTGATTAAA1950.1274501473846576No Hit
GGTATAGGGTCCGATCTAATATAGGGCAGCTTTTAAAAAATGTGTACTATTA1900.12418219488761512No Hit
ATTCTAGGGATAGAAAATGATACGATGTTTCATTAAAAAATTTAAAATAGAA1860.1215678328899811No Hit
GAAATGGGGATGAAACCTTCTTCAGAATAGATCTCATATTAGGCCATAAAAT1830.11960706139175561No Hit
TGCGGATTGGGAATGACACAGAAGACAGCAAAGAAACTCCCACTGACCACAT1800.11764628989353011No Hit
CAACTAGGGAGCATTAAATTTAATTAATGTCTAATTTGTTTACTTGGTTTAG1760.11503192789589611No Hit
TACAGTGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1760.11503192789589611No Hit
TACTGGGGATCCAAACAACAGGTACAAATCGTATTGACACTATTCAACAAGG1750.1143783373964876No Hit
CTACACCGGGATGACTATGCAGTTTCTAAACATACACATAGAAGCTGAGTCT1740.11372474689707911No Hit
AATAGAGGGACAACTTTCATGCTTTTTCTTTATTTCTTGTATGCAGAATAAG1740.11372474689707911No Hit
CAAGCAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1720.11241756589826211No Hit
ACGCTGGGGCAACTCCACCTCCTGGTGTAGGAGTGGCAACATCATGTTGACA1710.1117639753988536No Hit
GTTAAAGGGGGTAAAGGCTATGAAATCCACAGTGATTCCCACCCTGGCCACA1700.1111103848994451No Hit
TTATCTGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1690.1104567944000366No Hit
CTTCTCTAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGG1690.1104567944000366No Hit
CTTATACACATCTCCGAGCCCACGAGACTTTCCGAAATCTGTCTCTTATACA1680.10980320390062809No Hit
CATGAAGGGATAGCTTACGGTAGTAATGGCATGGATTAATTTTATTTGCATG1680.10980320390062809No Hit
AGAACAGGGGATGCTTTGATTGTTATTCCTGGGCTTCTGGGCTTATTTACAA1670.1091496134012196No Hit
TCCGCAGGGCTGTGCTTTTGTATGTATTCATTTAAATTGTCAAAAAGGATGT1660.1084960229018111No Hit
TTATAGGGGAACAACTGCTAGGGAGGGTTGGTTTTCTCTGAGTGCATGATTT1660.1084960229018111No Hit
GTTTACGGGAGCATTGTGATAGGTTATTTACCCCGTGATGAAGGGAAGAGAG1660.1084960229018111No Hit
AGGCATGGGCCTAAAATCAATTCTCCATTTTTTATCATATGTGGGATTTGTT1640.1071888419029941No Hit
ACAAGCGGGGGAAACCAGTGCTAAGAATGCAACGAATGTAGAACAGTCTTTC1640.1071888419029941No Hit
TAAATAGGGTAACTAATTCAGTTTGGGATTTAATTATACAATTAAATTATGA1630.1065352514035856No Hit
CACTGCGGGCAGTGGCCAGATGAGCTAAATTAAATCACAAAAGCAGATGCTT1620.1058816609041771No Hit
CAGATAGGGTGAGTTTGAAATTGTTAGCATCATTCAACTAATATGCGTATAG1610.10522807040476859No Hit
AACCGTGGGGCCTACAGACAGACATACATAGACATTTCAAAATTAATAGCTT1600.1045744799053601No Hit
TTCCTTGGGATAAAAGAGGATACAAACAAATGGAAGAACATTCCATGCTCAT1600.1045744799053601No Hit
TGCAACGGGAGGAAGGAGGAAGAAAAATTTCTCCTGAAGAATTCATTGGAAA1590.1039208894059516No Hit
GTGCTTGGGAGAGCTTTCCTCTTTACTTCAAACAGCAAAAAAGTGGGGGGCA1570.10261370840713459No Hit
GCACCTGGGGGCACGTAATCGCCGAGGGCACGTGCATGCCCCCTGGTTAAGA1570.10261370840713459No Hit
GCATTAGGGAATTAGCCTGAAAAATATTTCAGGTAATGGTGTGGATTGGCCT1570.10261370840713459No Hit
CTGACAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1560.1019601179077261No Hit
GGGCCAGGGTATTGTACCACTGCGCTCCAGCCTGGGCAACACAGCGAGAGCG1560.1019601179077261No Hit
GTAATAGGGTAACAAGGATGTGACCCATCCACGTATGCGGCGCTATATCAAG1550.1013065274083176No Hit
GCGCATGGGCCCATGTTCTTTCCCAGAACAAGTAGAAAGATAAGTGTCCTCC1540.10065293690890909No Hit
CTAGACGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1540.10065293690890909No Hit
ACACTGGGGTACACTGGATAGCATATTAAACACCTTGGTGAATTATGGTGCT1540.10065293690890909No Hit
GTCAGTGGGCCTCTCAATGTAGGTTTTAAAAAGACAGGGAAGTGCAGCACAT1540.10065293690890909No Hit
TAGTCGGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1540.10065293690890909No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)