FastQCFastQC Report
Sat 31 Dec 2022
SRR5846992.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5846992.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences127659
Sequences flagged as poor quality0
Sequence length52
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGCTGAGGTATCTCGTATGCCGTCT3820.29923467988939284TruSeq Adapter, Index 2 (95% over 21bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACGCTGAGGTATCTCGTATGC3510.2749512372805678No Hit
TTGTCAGGGGATTTCCGACCCGGGCAAGATGGCAGCGGCGCTGCGCGTGCGT1550.12141721304412537No Hit
TATGGCAGGGAAGCCAGGAAATAACAAGTATTGGCAGGGATGTAGAGATACT1550.12141721304412537No Hit
TAACATGGGAGAGTGTGGTCAGGCGGCTCGGACTGAGCAGGACTTTCCTTAT1480.115933855035681No Hit
ATCAGTGGGGGCCATTTTGCTGGTTGCATTACTGGGTAATCGGGGCCCTGGC1470.1151505181773318No Hit
GCAAATGGGAACTCCACTGATCACTCAGCTGGAGCAGAAGGAAGTTTGTCAT1460.1143671813189826No Hit
TCTGCTGGGAAGCCAATATCTCTAATGAATATTGATGCGAAAATCCTCAGCA1440.11280050760228422No Hit
AATGTAGGGAAAGACAGCTTTACTACTCCCTTTTCCCTTTGGCTTTGTTATG1430.11201717074393501No Hit
TATGCTGGGAGCTTTTCAGTGCATCCCACCTCCCTGTCGCCCCCATGCTCGG1430.11201717074393501No Hit
TGCTCAGGGCATCACAGTAAATTGTTTGAAAATTATTACAAAAGCACTAATC1410.11045049702723662No Hit
CGTAATGGGCTTTCCCGCGCGACCGGCGAGGGAGGAAGAAGCGCGAAGAGCC1410.11045049702723662No Hit
ATTGTGGGGCTGGAGCAGGGTGGCCGTGGCAGCGTCCTGCAGTTCATGCCCT1370.10731714959383984No Hit
CGGCTTGGGAGCAACCACAGGTGCCCTACACTTCAACACATCTGGAAAGTAA1360.10653381273549065No Hit
GCCTTGGGGCCTAGTTACAGATTAGTCTTAAAGAATTGCATTAACTTAAAAA1350.10575047587714145No Hit
TCATATCGGGATGCCTGGCACAGCCAGGAAGGAGCTGCCCATGGGATTGTCA1350.10575047587714145No Hit
CTTTTTGGGGCCGCCTCCTCTGCAACGCCGGGGCCAGAGTCTTAAAACCGAG1340.10496713901879225No Hit
GTTTGAGGGTCTTTTTTTGGGATTTTGGCACAAGACAAAATACTCATGCTAA1340.10496713901879225No Hit
TTTGCTGGGATCAAACTCAAACTACGCCCTGATCGGCGCACTGCGAGCAGTA1340.10496713901879225No Hit
GGGTACTGGGGGAGTGCAGGGGGACCATAGAGACGAAGAGGTGGCGGCGGCC1330.10418380216044305No Hit
CTACATAAGGGACCTGGAAACTAAGTGGATTACCCAAACACCGCGTGATTGG1330.10418380216044305No Hit
ATAAGCGGGATGGAATGGAATGGAGTGGAATGGAATGGACTCGAATGGTATG1330.10418380216044305No Hit
GGCGCTGGGAGTTTTATGTGTTGCTGTTTGTATTTCTTTCACAGTTTTGATA1310.10261712844374467No Hit
GCAGCTGGTCTGTGCGCGGGAGTGGTGAACGTGGGCTGCGGGCCAGCCGAGG1310.10261712844374467No Hit
AAGTGAGGGCTCCAGCTCTGGGCTCCGCGGCCCCCCGGGGGACAGCTCCTTC1310.10261712844374467No Hit
GATTATGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1300.10183379158539548No Hit
AATTAAGGGACTGAGTCTTACCTCGTTGTGGCGTCAGAACCGCCGTCGCTCG1300.10183379158539548No Hit
CAGTTTGGGTCTGTGCTCGGGAACCACAGGTCTACAAGAGCGTTTTTAGTAA1300.10183379158539548No Hit
TACGCGGGAACCCACAGAATGAAGAAAATATTTGCAGACTACCCAACTGATG1300.10183379158539548No Hit
CTTACTGGGATACCTGCAGCTGTGCTGGCCGGCTTCACAGGAAGTGGACCTA1290.10105045472704627No Hit
TTGACAGGGCTCTTTTTCCGGCTGGAACCATGGAGGGTGTAGAAGAGAAGAA1290.10105045472704627No Hit
TTCACCGGGAGCATGGAAGGTGCAAAACTCAGTATTTCTACAATTGCAGCTA1290.10105045472704627No Hit
CTTAGTGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1280.10026711786869708No Hit
GCATATGGGAACCATTAACACTTCGGTTCTGTTGCTCATTATGGAAACCATG1280.10026711786869708No Hit
AATTCTGGGCGATCTGACTAGGGGAAGTGCTCTTATCCCAGATCACCAGTCC1280.10026711786869708No Hit
TGCTCTGGGATCAAGCTGCGGCCTCCAGGAGCTGAGAAACCCTCCTGGCTAG1280.10026711786869708No Hit
TGGCACGGGATAAGTAAAACTATTTCTTTAAAAAGTATGTTCTCAAATGAGA1280.10026711786869708No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)