FastQCFastQC Report
Sat 31 Dec 2022
SRR5846991.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5846991.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences127819
Sequences flagged as poor quality0
Sequence length52
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGCTGAGGTATCTCGTATGCCGTCT3740.2926012564642189TruSeq Adapter, Index 2 (95% over 21bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACGCTGAGGTATCTCGTATGC3390.26521878593949255No Hit
TATGGCAGGGAAGCCAGGAAATAACAAGTATTGGCAGGGATGTAGAGATACT1870.14630062823210946No Hit
TACGCGGGAACCCACAGAATGAAGAAAATATTTGCAGACTACCCAACTGATG1760.13769470892433833No Hit
TGCTCAGGGCATCACAGTAAATTGTTTGAAAATTATTACAAAAGCACTAATC1710.133782927420806No Hit
TTCACCGGGAGCATGGAAGGTGCAAAACTCAGTATTTCTACAATTGCAGCTA1600.12517700811303484No Hit
CGTAATGGGCTTTCCCGCGCGACCGGCGAGGGAGGAAGAAGCGCGAAGAGCC1540.12048287030879602No Hit
ATAAGCGGGATGGAATGGAATGGAGTGGAATGGAATGGACTCGAATGGTATG1540.12048287030879602No Hit
GCCTTGGGGCCTAGTTACAGATTAGTCTTAAAGAATTGCATTAACTTAAAAA1490.1165710888052637No Hit
TCTGCTGGGAAGCCAATATCTCTAATGAATATTGATGCGAAAATCCTCAGCA1480.11578873250455723No Hit
GCAGCTGGTCTGTGCGCGGGAGTGGTGAACGTGGGCTGCGGGCCAGCCGAGG1460.11422401990314429No Hit
CTGCGTGGTTCTTGGGAAAATTATAAAATCTGTTCCTGGAAAACTAATGAAA1450.11344166360243782No Hit
GTAGTTGGGTTATGTTGAACTTGACACAGGAGAAAAGCTTTTCCACAGAATT1430.11187695100102488No Hit
ACATTGGGGAAACTTTATTTATGGCAGTATTCATAATTAGCCTGAAATGCAT1420.1110945947003184No Hit
CACTGTAGGGCAGCAGAGGAACCTTCAACCCGGAAAAGGGCAAACAAAAATT1400.10952988209890549No Hit
GGGTTGGGGGGGGGCCTGAGGTGCCAGGGTTCACAGACAGGGTTTCCCACCA1400.10952988209890549No Hit
CCATCGGGGCATGCCATCTTCTCCAGCCACCCCAGCCAGTGGAACCAAGGTA1390.10874752579819902No Hit
AGCCGGGAACAATGTCATTGGAAAGCATCCTCTTTGGTTTTTTGGTTATCAC1380.10796516949749255No Hit
AATTAAGGGACTGAGTCTTACCTCGTTGTGGCGTCAGAACCGCCGTCGCTCG1380.10796516949749255No Hit
TGTTATGGGCGATCTTTACATGGGCTTGAAGTCTGTACAGATTCAGCCTCAG1380.10796516949749255No Hit
TTGTCAGGGGATTTCCGACCCGGGCAAGATGGCAGCGGCGCTGCGCGTGCGT1370.10718281319678609No Hit
ATCAGTGGGGGCCATTTTGCTGGTTGCATTACTGGGTAATCGGGGCCCTGGC1370.10718281319678609No Hit
CAGCACGGGTACTTCAAGATGAGTTTATTGTTTTCATTTGTATTGCAAAAGT1360.10640045689607962No Hit
TTAAACCGGAGATGTACAGTTGTTACAACTCTGTCTACAGCAGTTCCCTGAC1360.10640045689607962No Hit
TCCGGAAGGGTATCTGTTATCTGAAAAGCAGTTATCTTGGCCAGTTTGAATT1350.10561810059537315No Hit
TGTTGTGGGGTCCGAGAGCCGCAGCGGCAGCGGCGCGTCCTGCCTGCAGAGA1350.10561810059537315No Hit
ATTGTGGGGCTGGAGCAGGGTGGCCGTGGCAGCGTCCTGCAGTTCATGCCCT1340.10483574429466667No Hit
TTGGTCAGGGTACCATGAGAACAGTATAGGGGAAACTGCCCCCATGATTCAA1340.10483574429466667No Hit
GGGTCCGGGATCTAGCATTGCTCATAAAAGTACTCATGTTCATCCTTTTTGG1340.10483574429466667No Hit
AAAGATGGGTTGGGAAGTGTGAATCCTTCAACTTTGTTTTTCCTTTTCAAGA1340.10483574429466667No Hit
TGAATAGGGGTCCTCGCCTGTCTCAGCAGCGGGACCCCTGTTTCCTCTTTGT1330.1040533879939602No Hit
TTCTGAAGGGGCTGGGCAACCCTGGAGCTGAATCTGGCCCTCAGACTTGTTT1330.1040533879939602No Hit
GTCATAGGGATGCCCGGAAGGACTCCTGGAAATAGCCCAGGAAAATGAGAAG1310.10248867539254726No Hit
GTAACTGGGCTATTTTTTCTGGGAGAGGGGGGGATTGATTAGAAAAAAATGT1310.10248867539254726No Hit
GTTTGAGGGTCTTTTTTTGGGATTTTGGCACAAGACAAAATACTCATGCTAA1310.10248867539254726No Hit
GAACTAGGGATCTATGAGATGACAATGTCATTTTTGGTTTTAGTTTTGTTTA1310.10248867539254726No Hit
TCATATCGGGATGCCTGGCACAGCCAGGAAGGAGCTGCCCATGGGATTGTCA1300.10170631909184082No Hit
TATCATGGGGGGCAAGCTCAACGCGGCCATCAGCTTCTTGTGTGGTGTCATT1290.10092396279113434No Hit
TTTGCTGGGATCAAACTCAAACTACGCCCTGATCGGCGCACTGCGAGCAGTA1280.10014160649042787No Hit
TATCTCGGGAGATGGATTTCTTTCACTTAGCAATATACATTTAACCTCTATC1280.10014160649042787No Hit
TGCTCTGGGATCAAGCTGCGGCCTCCAGGAGCTGAGAAACCCTCCTGGCTAG1280.10014160649042787No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)