FastQCFastQC Report
Sat 31 Dec 2022
SRR5846990.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5846990.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences125510
Sequences flagged as poor quality0
Sequence length52
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGCTGAGGTATCTCGTATGCCGTCT3470.2764719942634053TruSeq Adapter, Index 2 (95% over 21bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACGCTGAGGTATCTCGTATGC3420.2724882479483706No Hit
TGCTCAGGGCATCACAGTAAATTGTTTGAAAATTATTACAAAAGCACTAATC1870.14899211218229622No Hit
TATCATGGGGGGCAAGCTCAACGCGGCCATCAGCTTCTTGTGTGGTGTCATT1600.12747988208110908No Hit
TATGGCAGGGAAGCCAGGAAATAACAAGTATTGGCAGGGATGTAGAGATACT1570.12508963429208828No Hit
TCATATCGGGATGCCTGGCACAGCCAGGAAGGAGCTGCCCATGGGATTGTCA1570.12508963429208828No Hit
AATTTAGGGAAGGGGCTAAGGGCTTCCAGCACCAACGGATGACCAACGGGGC1470.11712214166201895No Hit
GGCGCTGGGAGTTTTATGTGTTGCTGTTTGTATTTCTTTCACAGTTTTGATA1410.11234164608397738No Hit
GTAGTTGGGTTATGTTGAACTTGACACAGGAGAAAAGCTTTTCCACAGAATT1410.11234164608397738No Hit
TCTGCTGGGAAGCCAATATCTCTAATGAATATTGATGCGAAAATCCTCAGCA1410.11234164608397738No Hit
ACAGCAGGGATCCTTCTCTCTCAGCTCTAATATATAAGGACGAGAAGCTCAC1400.11154489682097045No Hit
CGTAATGGGCTTTCCCGCGCGACCGGCGAGGGAGGAAGAAGCGCGAAGAGCC1400.11154489682097045No Hit
CGGCTTGGGAGCAACCACAGGTGCCCTACACTTCAACACATCTGGAAAGTAA1380.10995139829495658No Hit
GCAGCTGGTCTGTGCGCGGGAGTGGTGAACGTGGGCTGCGGGCCAGCCGAGG1380.10995139829495658No Hit
TTAAACCGGAGATGTACAGTTGTTACAACTCTGTCTACAGCAGTTCCCTGAC1360.10835789976894271No Hit
ATTGTGGGGCTGGAGCAGGGTGGCCGTGGCAGCGTCCTGCAGTTCATGCCCT1350.10756115050593579No Hit
TCCAGGGACTGGAAAGTCTTTTTAACATAAAGTGGCAGAATCTGGGATCTTG1340.10676440124292885No Hit
TGTTATGGGCGATCTTTACATGGGCTTGAAGTCTGTACAGATTCAGCCTCAG1340.10676440124292885No Hit
TTCACCGGGAGCATGGAAGGTGCAAAACTCAGTATTTCTACAATTGCAGCTA1340.10676440124292885No Hit
CACTACGGGCGCTCTTCCGATCTCTAAGCGGGAACGCAGCACAGGCACAAAA1340.10676440124292885No Hit
TGAACAAAGGGAGATGGAAGGAAGAACTTGTGTGCTTAGACCTGACGCTGGG1330.10596765197992192No Hit
CAAAATTGGGGGAAGCAATACGAAAAGGTATTCAGCTACGCAAAGTAGAAGA1330.10596765197992192No Hit
TATACAGGGAGAATTCAAAAAAACATGTTTGAAATTAATAATGAAATGGGTA1320.10517090271691498No Hit
CTATGTGGGGATATGCCCTTGGCTGAGGGAGCACCATACTCCAGTAAAGCTG1320.10517090271691498No Hit
TGAATAGGGAAGAATCCATGGGACACTTTCAAAAACCCCAGTGAATACCCGC1310.10437415345390805No Hit
CTTAGTGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1290.1027806549278942No Hit
GTCCGCGGCAGCTGTAGTAAGCCATTATAGTTTAATGTAAAGTTTAAAATGC1290.1027806549278942No Hit
GTCATAGGGATCAGAGCTCTTAGTAGACTCTGTTCCTGGTGAATTAACAGAA1280.10198390566488727No Hit
TTCTGAAGGGGCTGGGCAACCCTGGAGCTGAATCTGGCCCTCAGACTTGTTT1280.10198390566488727No Hit
GACTGTCGGGCAGCTAAAAGAGCACACCCGTCTATGTAGCAAAATAGTGGGA1280.10198390566488727No Hit
ATGCATGGGATCGCATTGGGCAGCAAAAGCAGGGTGGGGTCCAGCGAAATCA1280.10198390566488727No Hit
AGGCTAGGGTGCGGGGCCAAGTGTTGCGGCGACGCACCTCACGTCGAGAATC1270.10118715640188033No Hit
TGTTGGGGGCCTACTGAAATCTTGCCATTCCTCTACCAACTTTCAGAAAAAT1260.1003904071388734No Hit
TTTGCTGGGATCAAACTCAAACTACGCCCTGATCGGCGCACTGCGAGCAGTA1260.1003904071388734No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)