FastQCFastQC Report
Sat 31 Dec 2022
SRR5846989.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5846989.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences289891
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACGCTGAGGTATCTCGTATGC8880.30632203138420994No Hit
CTTATACACATCTCCGAGCCCACGAGACGCTGAGGTATCTCGTATGCCGTCT8600.2966632285928159TruSeq Adapter, Index 2 (95% over 21bp)
CAGTGAGGTGGATAGATGTTATTCAACTCCTTCCAGTTGTCTTGAACAGCCT3190.11004136037338172No Hit
TGAGATGGGATAATCGTATCATATGGAACAAGGAAGAAAACAAGTGATGGAC3160.10900648864573236No Hit
CAACACGGGAGAGACCCTGGAAACACACTGAGATCTCCAATTGCTGCCTCCA3130.107971616918083No Hit
GCAAACGGGCTTTCGGAGAAGCTCATGGTGGGGGAAATCTGGAATTTATCTA3050.10521195897768472No Hit
CGCTATGGGGGAGGCCGACGGGGCGCCGTACGGCGGAGGCGGGGTTTCAGTG3020.10417708725003537No Hit
TTACGCGGGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCC3020.10417708725003537No Hit
GTTATCGGGCATTTCACGAAGTTTGATGTCTTGTTCTTCCATTGTTTCATCT2960.10210734379473664No Hit
TGATTAGGATGAATGGCTGGCTGCTTCTAGGAATATAAGATGCCTCAAGCAT2950.10176238655218686No Hit
AATACCGGGGGTAGCGGGCGACCTAGGCCGCGGGACCCGGACGGAGGTAGAG2920.1007275148245375No Hit
CTTGCAGGGAATGGTAGATGAGCAGCAAGTGTTTTTGAATGTTATTAATTCA2910.1003825575819877No Hit
TAATGAGGGGGTTCTGATAAATTTGACTGTTAAATTGCTATAGCTAGCAATC2910.1003825575819877No Hit
CTAGACGGGAATGGCATTCCTAATGCTTACCGAACGAAAAATTCTAGGCTAT2910.1003825575819877No Hit
AATACCGGGAAGAGCGGATTATGGGCGCAGCCATACTGCTGCCTTCTAGCGG2910.1003825575819877No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)