FastQCFastQC Report
Sat 31 Dec 2022
SRR5846988.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5846988.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences288124
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACGCTGAGGTATCTCGTATGC8850.30715941747303244No Hit
CTTATACACATCTCCGAGCCCACGAGACGCTGAGGTATCTCGTATGCCGTCT8700.3019533256514556TruSeq Adapter, Index 2 (95% over 21bp)
TTAGCAGGGGAGACGTTCTTCGCCGAGAGTCGTCGGGGTTTCCTGCTTCAAC3290.11418694728658493No Hit
CAGTGAGGTGGATAGATGTTATTCAACTCCTTCCAGTTGTCTTGAACAGCCT3220.11175743776984909No Hit
CGCTATGGGGGAGGCCGACGGGGCGCCGTACGGCGGAGGCGGGGTTTCAGTG3190.11071621940553372No Hit
CAACACGGGAGAGACCCTGGAAACACACTGAGATCTCCAATTGCTGCCTCCA3130.10863378267690299No Hit
CTTGCGCGGGGACCAGTGCACCGGTCTTCAGGGCTTCTTGGTTTTCCACAGC3120.10828670988879786No Hit
GTCTACGGGATCAGCTGCTTGGTCAGGCTGGACGCCATGTTCCGTGCCTTCA3110.10793963710069275No Hit
GCAAACGGGCTTTCGGAGAAGCTCATGGTGGGGGAAATCTGGAATTTATCTA3040.10551012758395689No Hit
TCATGAGGGATAGATGAAACGGAAAAATTTTATGAAAAACAATACTTACCAA3010.10446890921964154No Hit
TTACGCGGGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCC2960.10273354527911592No Hit
ACGACGGGGCTATCAGTATATATACTAAAATCCACAAGTTTATCGCAGTACC2940.10203939970290568No Hit
ATACGGGCTGGTCAAAGAGGGAGTGGTGAAAGATCTCAGTGGAGGCATTGAT2940.10203939970290568No Hit
TGATCAGGGTGAATTTTGCCTCAGTATTTGAACTCTAGAAACAATGAAACAT2930.10169232691480057No Hit
ACAACCGGGATGCTTATGGCTTGGAGAAATTTCTTAGTTGGGTGAATTAAAG2920.10134525412669544No Hit
ACTGTTGGGAGGAGGGAAGATGGCGGACGAGGAGAAGCTGCCGCCCGGCTGG2900.1006511085504852No Hit
GCGCTTGGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCC2900.1006511085504852No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)