FastQCFastQC Report
Sat 31 Dec 2022
SRR5846987.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5846987.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences283715
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACGCTGAGGTATCTCGTATGC8630.30417848897661387No Hit
CTTATACACATCTCCGAGCCCACGAGACGCTGAGGTATCTCGTATGCCGTCT8260.291137232786423TruSeq Adapter, Index 2 (95% over 21bp)
CAGTGAGGTGGATAGATGTTATTCAACTCCTTCCAGTTGTCTTGAACAGCCT3260.11490404102708705No Hit
CAACACGGGAGAGACCCTGGAAACACACTGAGATCTCCAATTGCTGCCTCCA3120.10996951165782563No Hit
TCATGAGGGATAGATGAAACGGAAAAATTTTATGAAAAACAATACTTACCAA3050.10750224697319492No Hit
CGCTATGGGGGAGGCCGACGGGGCGCCGTACGGCGGAGGCGGGGTTTCAGTG2980.10503498228856423No Hit
GCAAACGGGCTTTCGGAGAAGCTCATGGTGGGGGAAATCTGGAATTTATCTA2950.10397758313800821No Hit
TGATCAGGGTGAATTTTGCCTCAGTATTTGAACTCTAGAAACAATGAAACAT2920.10292018398745219No Hit
GTTATCGGGCATTTCACGAAGTTTGATGTCTTGTTCTTCCATTGTTTCATCT2870.10115785206985883No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)