FastQCFastQC Report
Sat 31 Dec 2022
SRR5846985.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5846985.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences147714
Sequences flagged as poor quality0
Sequence length52
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGCTGAGGTATCTCGTATGCCGTCT5440.3682792423196176TruSeq Adapter, Index 2 (95% over 21bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACGCTGAGGTATCTCGTATGC5000.33849195066141324No Hit
CTGACTGGGTGGTTATGGATCAGGAGACAGTGAAGATGAGAGGAGTGACAGA2690.18210866945584034No Hit
AATGCCGGGTGCCCTTTGATGCTTGATTCCAGTGATTGATTCCCTTTGTTGC2340.1584142329095414No Hit
CAGCCTCGGGGGAGAAGAGAACTGTTCTACACAATAAAAAATTATTGAGAAA2260.1529983616989588No Hit
ATCAGAGGGAGAGCAAAAGAGGCTTGAAGAATATGAAAATAACCAGCCACAG2190.148259474389699No Hit
CACTACGGGGAATAAGAAATTATTTCTCAGAGGAAAGTTAAAGGAAAGTGAT2100.1421666192777936No Hit
AACTGGGTTAAATATAATGACTCATGCCTAAACCTCTTTCCAGATAAATTGA2060.13945868367250228No Hit
GCAAACGGGTCTGCGTACGGGATAGCAGCTTTATTCATAATTGCCAAAACTT1960.132688844659274No Hit
CACACGGGGGTCTCGTGGTGCGCCGTTTTTTAAGCCGGTCTGAAAAGCGCAA1940.13133487685662834No Hit
TCGCAGGGAGGGTAAAGTGTATACAATCAAAGTTAAGTTGTAATCAGCTTAA1940.13133487685662834No Hit
GTACCAGGGGGAAGAGCTCTCGGCTGTGCAGTGGTACCGTCGGGGCCTGGGC1900.12862694125133706No Hit
GCACAGGGGGATCTGCTCACGGGCTGATGCTTCAGTGTTATAAATTCAACAT1900.12862694125133706No Hit
CATAAGGGAGAGCTAACATGGATGGGTTGAAGAAGAGAGACAAAAAGAACAA1900.12862694125133706No Hit
ACTTGCGGGGAGCTGACCATGGAAGCGAATGGGTTGGGACCTCAGGGTTTTC1870.12659598954736856No Hit
ATTCCAGGGTCACACATTCCATACACACACACATTCCATACGCACAAACACA1840.12456503784340008No Hit
ATTGCAGGGAGTTTGGCATTTTTGGCCCTTTTCAATACTAATCAAACTTACT1840.12456503784340008No Hit
TACGCAGGGGAGTTGAAAAAATTTAAAGCAACTGTAATAATGACAAAAACAT1830.12388805394207725No Hit
TTGCTGGGGGATGTCAAGATAACACAACTACAACTACTAAGTCTGAAGATGG1820.12321107004075443No Hit
GTAGGAGGGGTGAATATTTAGTATCGTGAATATTAAACCTTTGCAGCTGAGT1810.1225340861394316No Hit
GCCATTGGGATGGCCTATAATGTTCTAAGTTCCAGAATGAAAAGATCTGTAA1800.12185710223810878No Hit
GCTTCTGGGGTCGACGACGGTGGCTACTATGACGGCATGAGCGTGGAGGCGC1790.12118011833678595No Hit
CGACTTGGGCTGCCTGGCTCCTGTAGCATCTGAAAGCCCTGAAGGAACTTGA1780.12050313443546312No Hit
ACTTCAGGGATCCAGAGTTACCAAATCTAGTATTTGAAGTCCTTAGGTTTGA1780.12050313443546312No Hit
TCAGCAGGGTAGCTTAAGATTTTAAGAGAAAATATGAAGACTTAGAAGAGTA1780.12050313443546312No Hit
TTTAAAGGGCTTTGCACCCTGGGAACCTCTGACCATGTGCCTGCCTGTCCTT1780.12050313443546312No Hit
GGTATTGGGCAAGCTGTGTCAGAAGCCAAACAAGAAAACGAAAATATGAGAA1760.11914916663281748No Hit
AAGCATGGGATTGTGCCAAGGACTTGAATGGATGATGTCACGACTTAAGATT1750.11847218273149465No Hit
TTCCTGGGGAAGCCTGTGTAAAATGCTACCAAATGGCAAAAAGCAACAATAA1750.11847218273149465No Hit
AATAAGGGGATCAAAAAGTTGAAATTATATGAAAGTCAGAGAAATGTGAACG1740.11779519883017182No Hit
CTCTCCTAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGG1730.117118214928849No Hit
ATGGCGGGAAGAATGAAGTGTTGAGAAAAGGCTATTTCAGGCATTCATGAAA1730.117118214928849No Hit
TGGTAAGGGAGCTGGGGATATAGCTATCCCAGAGAGATGAAAGATCTCTACA1730.117118214928849No Hit
GTTAGTAGGGCTTATATCAAAATCAGCTAGAGTTAGCATCTACATTATTGAA1710.11576424712620335No Hit
GCATTGGGGCTTCCTTTCCAACTTGGACGCTGCAGAATGGCTCCCGCAAAGA1700.11508726322488053No Hit
ATAACAGGGTAGGAAAAAAGGGTATGAAAATAAAATGGAGAGTGAGAGGTAT1700.11508726322488053No Hit
ATTTTGGGGAGGGGAGGAACCAGTTGTTCTGTAGCCTAGGAACTGCCTCAGT1680.11373329542223486No Hit
CGTAATGGGATTGACCAGGTAGGTAGGCACGTTGATCACAAACACCTTGAGG1680.11373329542223486No Hit
GGTTAGGGACAGCAAGTTCATCTCACATTGAGTTATTGTCCAAGACTGCCAT1680.11373329542223486No Hit
AGCGTAGGGCATTAATGACGTAGAAATAAAGTCTTTCCTAGTCAAGCAAATG1660.1123793276195892No Hit
TTACTAGGGCAAACTGTGAAGGTTTATGCTTCATTAATTATGACCATACTTT1660.1123793276195892No Hit
CTAGCCGGGATCTGAATTAGGGTCTCTTAAAAAAGACACCTAATGAAAGTGA1660.1123793276195892No Hit
TGTAACGGGGAGACGTTCTTCGCCGAGAGTCGTCGGGGTTTCCTGCTTCAAC1660.1123793276195892No Hit
TTGCGTGGGGCGGGAAAGAGAGAGGGAACGAGACAGGGAAAAAGAACGGGAA1650.11170234371826637No Hit
CTCGCGGGGACGTGGGCCGAGAGCTGACCCGCAAGAAGGGCATCCTCTTGCA1650.11170234371826637No Hit
CGCGCGGGCCGATCCAGAGAGGGAGGAATGAGGGTCGTGACCAACTCAGTAT1640.11102535981694354No Hit
AAAGTTGGGTATCCGCTGGCTCCTGTTTCCAATTGATTGAGGGATGCCCCCA1640.11102535981694354No Hit
CATAAAGGGTGTCTCAAATACCTGTCATTGGCTTCTCTCCTCTACAGTTTGA1630.11034837591562072No Hit
TTATCGGGATGCATATTTGCACTTCCAGATGGGTTGCATAAGAATCAGGTCC1630.11034837591562072No Hit
TGACAGGGAAGGGATAAAATTACAAATTGAAAGTCAGGGAAGACTTATCTAA1620.1096713920142979No Hit
TAAACGTGGGTGAGGTTCAATTGCAATCATGTTGGAGTCGAGTACGTAGAGT1610.10899440811297507No Hit
ACAATGGGACTTATGTAAATTTATGCAGCTGGATTGGATTTCTCCTCAGAAA1610.10899440811297507No Hit
CAATTTGGGGAGCCGGGTTGTATTTGGAAACGCGGAGTGAGTTTTTCCGTGC1610.10899440811297507No Hit
CTGATGGGATGTCATCAAGAAGTGCAGCTGAGTCCTTCTTGTTTATTTCTCT1600.10831742421165225No Hit
CAATACGGGATGACATTATATCTTAGATGTGTAATTTATGAACTACTACTGG1590.10764044031032942No Hit
CAAAATGGGGTTAAAAAGATACTGCTTCTACTTATTAAGCCATCCATCTACC1590.10764044031032942No Hit
GATCTGGGGATCTCTGATCTTAGATGCCACCTTATACCTTGCTGTACATATA1580.10696345640900659No Hit
TGTAAGGGGATAGTGGTTTGCTACTGTGCATATCTTCCCAGCTCCCTTTTCA1580.10696345640900659No Hit
TATGAGGGGGTTTTTTGTTTTTGTTTTTTGTTTTTTTAATTTGTAAAATCTG1580.10696345640900659No Hit
CTAGTTGGGGAGTGACTCAGACTTTGGTCAGAGTCAAGAGGAAGTGACCTTC1580.10696345640900659No Hit
CTTAATGCGGGGGGGAAAGCCAGGTTTGTTGACATCTTTAAAGAACATGGAA1570.10628647250768378No Hit
TGCACTGGGGAGTGACAGAGCATGTCTATTGCAAACAATTCTCTCAGTTACG1570.10628647250768378No Hit
GATAAAGGGGTTTTAATATTATTTTATAAAATGAACAATATTTCATTTTCCA1570.10628647250768378No Hit
CATGAAGGCAGAAGCGGTATTTTTAACAAGCTCCCAGGTAATTCTGATGTGC1570.10628647250768378No Hit
CATTTGGGGGTTTTGTAGATGTTTTCTGTAGGGACCAGCCCCACAGGGTTGG1570.10628647250768378No Hit
CTTTACGCGGGGGAGTGGAGAGAGCGAGCTGAGTGGTTGTGTGGTCGCGTCT1560.10560948860636095No Hit
CATTGTGGGGAAGGACTACATGTTCATTTGTCCAAATTGTCCCTTTAAAATA1560.10560948860636095No Hit
CCAAGACGATGTAGCTGTTTGTTTAGAAGTGGTTCAGCTCCATGTATGTTTT1550.10493250470503812No Hit
TTACCGGGGCTTGATTCCAGTGATTGATTCCCTTTGTTGCTTGAGGGCCATA1550.10493250470503812No Hit
AGACCAGGAAGTTTTCTTACTCATCTCTTCTTACAATGGGTCAGGCACCGGG1550.10493250470503812No Hit
CCACAAGGGGAATGAAGAGCCATTTTTCTTTTCTTTTTTTTAAGTCAGTCAT1550.10493250470503812No Hit
CAACATGGGTAAAAACACTCTCTGTATGCATTGTTTCATGGGAACTCCACCA1540.1042555208037153No Hit
CTGCCAGGGAGGCCTGCTGAGACTACAGGTCTGAGCCACTGTGCCTGGCCCA1540.1042555208037153No Hit
GTCAACGGGATTTTCCTTACCGAATATAGTGAGGAAGCGTCACAGAATAACA1540.1042555208037153No Hit
CAAACTCCTCTGACACCAGACCATCTGCCTGCCTTGGCTCCCCAAAGTGCTG1530.10357853690239247No Hit
TATCAAGGGCGGTGGGGGGCAGCTGGGAGGCCTGGTTCCAGGTGACCTTCTC1530.10357853690239247No Hit
ATTGATGGGGCCAAAACTTACCTATAAATTAAGAAGAGTTTTTATTACAAAT1530.10357853690239247No Hit
AATTCCGGGGTCGGAGAAAGGATGGACAGTGTGGCCTATTTGCTTTGATGTA1530.10357853690239247No Hit
AACTCGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1520.10290155300106965No Hit
GGATGTGGGGTATTCTGCATCAGTTTGCAGTTCACTGCAAATCTTTTACATT1510.1022245690997468No Hit
CATGCAGGGTGATGCTTCCATAGCACAGCTTCCTCTGATCCTCCTCTCTGCC1510.1022245690997468No Hit
ACTTCTGGGAAGCTGAGGCCCGTGGATTGCTTGAGCCCAGGAGTTTGAGAGC1510.1022245690997468No Hit
ATGCATGGGATTTCAGATTTATTCCAAATCTCCATTCTACCGAGCTGCCTCA1510.1022245690997468No Hit
CTTGATGGGGATGGAAGAAACCTCACTGATTAATTTTAAGAACCTTTTAGGG1500.10154758519842397No Hit
AACAACGGGAGGCGGCGGCTGTAGTGTTAGCTTTGGACGCCGCAGTAGCCGC1490.10087060129710115No Hit
CTATGTGGGCGACTTAAGAGATGGTGAATCTGGTGCACCATGCACTTTCCTG1490.10087060129710115No Hit
TATATGGGGTCAAATCCACAATGAGATACCTTATTATACCAGACAGAATTGT1490.10087060129710115No Hit
GGCTATGGGTCTGGCTCTGGGATTTCGCAGAAGCTTTTAGCATCGGATTATC1480.10019361739577833No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)