FastQCFastQC Report
Sat 31 Dec 2022
SRR5846984.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5846984.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences146671
Sequences flagged as poor quality0
Sequence length52
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGCTGAGGTATCTCGTATGCCGTCT4960.33817182674148266TruSeq Adapter, Index 2 (95% over 21bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACGCTGAGGTATCTCGTATGC4720.3218086738346367No Hit
CTGACTGGGTGGTTATGGATCAGGAGACAGTGAAGATGAGAGGAGTGACAGA2460.16772231729517084No Hit
ATAACAGGGTAGGAAAAAAGGGTATGAAAATAAAATGGAGAGTGAGAGGTAT2080.14181399185933144No Hit
ACTTGCGGGGAGCTGACCATGGAAGCGAATGGGTTGGGACCTCAGGGTTTTC2030.1384050016704052No Hit
AATAAGGGGATCAAAAAGTTGAAATTATATGAAAGTCAGAGAAATGTGAACG2010.1370414055948347No Hit
AATGCCGGGTGCCCTTTGATGCTTGATTCCAGTGATTGATTCCCTTTGTTGC1980.13499601148147894No Hit
GCACAGGGGGATCTGCTCACGGGCTGATGCTTCAGTGTTATAAATTCAACAT1940.13226881933033796No Hit
GCAAACGGGTCTGCGTACGGGATAGCAGCTTTATTCATAATTGCCAAAACTT1930.13158702129255273No Hit
CAATTTGGGGAGCCGGGTTGTATTTGGAAACGCGGAGTGAGTTTTTCCGTGC1890.12885982914141175No Hit
GGTATTGGGCAAGCTGTGTCAGAAGCCAAACAAGAAAACGAAAATATGAGAA1830.12476904091470024No Hit
TTGCTAGGGAAGACAGAGCAAAATGCTTTGAAAAGAATGAGGCCATACAGGC1820.124087242876915No Hit
AACTGGGTTAAATATAATGACTCATGCCTAAACCTCTTTCCAGATAAATTGA1820.124087242876915No Hit
CAGCCTCGGGGGAGAAGAGAACTGTTCTACACAATAAAAAATTATTGAGAAA1810.12340544483912974No Hit
CAAAATGGGGTTAAAAAGATACTGCTTCTACTTATTAAGCCATCCATCTACC1800.12272364680134451No Hit
TTCCTGGGGAAGCCTGTGTAAAATGCTACCAAATGGCAAAAAGCAACAATAA1800.12272364680134451No Hit
ATCAGAGGGAGAGCAAAAGAGGCTTGAAGAATATGAAAATAACCAGCCACAG1790.12204184876355925No Hit
CATCTAGGGGTGTATCAGGGATGAGATTTCTACTGCACACCCAACTTGCAAG1780.12136005072577402No Hit
TCGCAGGGAGGGTAAAGTGTATACAATCAAAGTTAAGTTGTAATCAGCTTAA1760.11999645465020352No Hit
CACTACGGGGAATAAGAAATTATTTCTCAGAGGAAAGTTAAAGGAAAGTGAT1760.11999645465020352No Hit
CTAGTTGGGGAGTGACTCAGACTTTGGTCAGAGTCAAGAGGAAGTGACCTTC1750.11931465661241826No Hit
TATGAGGGGGTTTTTTGTTTTTGTTTTTTGTTTTTTTAATTTGTAAAATCTG1730.11795106053684777No Hit
GTTAGTAGGGCTTATATCAAAATCAGCTAGAGTTAGCATCTACATTATTGAA1710.11658746446127728No Hit
TCAGCAGGGTAGCTTAAGATTTTAAGAGAAAATATGAAGACTTAGAAGAGTA1710.11658746446127728No Hit
TTTAAAGGGCTTTGCACCCTGGGAACCTCTGACCATGTGCCTGCCTGTCCTT1700.11590566642349204No Hit
GCTTCTGGGGTCGACGACGGTGGCTACTATGACGGCATGAGCGTGGAGGCGC1690.11522386838570678No Hit
TTACCGGGGCTTGATTCCAGTGATTGATTCCCTTTGTTGCTTGAGGGCCATA1690.11522386838570678No Hit
ATTGCAGGGAGTTTGGCATTTTTGGCCCTTTTCAATACTAATCAAACTTACT1690.11522386838570678No Hit
GTTCTCGGGGATTTCATAAACAAGATAGAAAAATCCAGTCATGAAGAAAAAC1660.11317847427235103No Hit
TGTTTGGGGGGACATCTTCAGGGAGTTATATCGCTCAGGGAACTAACATCTT1660.11317847427235103No Hit
ATTCCAGGGTCACACATTCCATACACACACACATTCCATACGCACAAACACA1650.1124966762345658No Hit
TTGCTGGGGGATGTCAAGATAACACAACTACAACTACTAAGTCTGAAGATGG1630.11113308015899531No Hit
GCCATTGGGATGGCCTATAATGTTCTAAGTTCCAGAATGAAAAGATCTGTAA1630.11113308015899531No Hit
CCAAGACGATGTAGCTGTTTGTTTAGAAGTGGTTCAGCTCCATGTATGTTTT1630.11113308015899531No Hit
GATCTGGGGATCTCTGATCTTAGATGCCACCTTATACCTTGCTGTACATATA1620.11045128212121005No Hit
TACGCAGGGGAGTTGAAAAAATTTAAAGCAACTGTAATAATGACAAAAACAT1620.11045128212121005No Hit
ATTGATGGGGCCAAAACTTACCTATAAATTAAGAAGAGTTTTTATTACAAAT1610.10976948408342481No Hit
CATTGTGGGGAAGGACTACATGTTCATTTGTCCAAATTGTCCCTTTAAAATA1610.10976948408342481No Hit
AAGGCTGGAGTGCAGTGGCACAATCTCAGCTTACTGCAACTTGTGCCAACCA1610.10976948408342481No Hit
GATAAAGGGGTTTTAATATTATTTTATAAAATGAACAATATTTCATTTTCCA1610.10976948408342481No Hit
ATATTTGGGCGAGATGAGCTGCACACAGAGCAGGATGCCATGCTGGTGGACA1600.10908768604563955No Hit
CGGTTTGGGATATGAATGTGATTAAAATGATGACGCAATTGCTGCTGCAACT1600.10908768604563955No Hit
CACACGGGGGTCTCGTGGTGCGCCGTTTTTTAAGCCGGTCTGAAAAGCGCAA1600.10908768604563955No Hit
TCAAGCGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1590.10840588800785432No Hit
ATTAGCGGGGTGTGTGGCACTGGTGTGGATTCAGATTCCTACTCCATGCCTT1590.10840588800785432No Hit
TGCAAGGGAGATCTGTTCTAAATCTTGAGATTTTCCTGCTATATTATCTGTC1570.10704229193228383No Hit
GGTACTGGGACTCTTTGTGATCCCTAGTTGTGTTTTGGCTAAGCGCTCAATG1560.10636049389449857No Hit
TGGCAAGGGGCAGCAAATCTTGTATCTATTTAAAATGTTTTGGGCTGCAAAG1560.10636049389449857No Hit
TATATGGGGTCAAATCCACAATGAGATACCTTATTATACCAGACAGAATTGT1560.10636049389449857No Hit
CTCGCGGGGACGTGGGCCGAGAGCTGACCCGCAAGAAGGGCATCCTCTTGCA1550.10567869585671333No Hit
CATAAAGGGTGTCTCAAATACCTGTCATTGGCTTCTCTCCTCTACAGTTTGA1550.10567869585671333No Hit
TCTTATGGGATTATTTATTTTTTCTAAGTTCCAGAATAATAGTGTCATTATT1550.10567869585671333No Hit
AACAACGGGAGGCGGCGGCTGTAGTGTTAGCTTTGGACGCCGCAGTAGCCGC1540.10499689781892807No Hit
ACTTCAGGGATCCAGAGTTACCAAATCTAGTATTTGAAGTCCTTAGGTTTGA1540.10499689781892807No Hit
ATTTAAGGGCTTCATTGAAAGTGTACTTCAACAAGTCCCTTTAAGTTTGACC1540.10499689781892807No Hit
CGCTTAGGGATTCCAGCCAAACTGGTCTGCCTGGTGCTCCTCAAACTCCAAG1540.10499689781892807No Hit
TAACGGGAACTGTTGAAGATGAGAAACTTCAAGGCAAGATTAACGATGAGGA1540.10499689781892807No Hit
TTACGCGGGAGATAAGGAAATATCTACAGAGGAGTATTTGACTTGATTTGGG1540.10499689781892807No Hit
CTTAATGCGGGGGGGAAAGCCAGGTTTGTTGACATCTTTAAAGAACATGGAA1530.10431509978114284No Hit
AGCGTAGGGCATTAATGACGTAGAAATAAAGTCTTTCCTAGTCAAGCAAATG1530.10431509978114284No Hit
CAGACAGGGATTTCTTCTCATCTCCATTGTAAGCTGTTTTTCTGTCATTTCT1530.10431509978114284No Hit
AGACCAGGAAGTTTTCTTACTCATCTCTTCTTACAATGGGTCAGGCACCGGG1530.10431509978114284No Hit
CTTGATGGGGATGGAAGAAACCTCACTGATTAATTTTAAGAACCTTTTAGGG1530.10431509978114284No Hit
CATAAGGGAGAGCTAACATGGATGGGTTGAAGAAGAGAGACAAAAAGAACAA1530.10431509978114284No Hit
CAAACTCCTCTGACACCAGACCATCTGCCTGCCTTGGCTCCCCAAAGTGCTG1520.10363330174335758No Hit
ACAATGGGACTTATGTAAATTTATGCAGCTGGATTGGATTTCTCCTCAGAAA1520.10363330174335758No Hit
TATGCAGGGTCCTTTAAAAAAATGTGCTGTTAAGCCAGTATTAGGTATCTTT1520.10363330174335758No Hit
GCTGTGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1520.10363330174335758No Hit
AACTTAGGGTGACTTCCAAGGGCCCTTCTAAAATTCTGTGAGTCCATGATTT1520.10363330174335758No Hit
GTGCTTGGGTAGACCTAGGCTTTGAAAGGAAAAATAAGTCAGCATCCTTTAC1510.10295150370557235No Hit
ATGATGGGGGAGCAACAGAATAAGACTGTCTCAAACAAACAAAAAAAGCATT1510.10295150370557235No Hit
TCAACTGGGTGGAGAAACTAAAAATGTTGTACAACTGACCGAAAGAAAACCC1500.10226970566778709No Hit
TTTACGGGATTGCTGTCCAGGCTGCAGTAAGCATTTTAAGAGTGGGAACTTT1500.10226970566778709No Hit
GCATTGGGGCTTCCTTTCCAACTTGGACGCTGCAGAATGGCTCCCGCAAAGA1500.10226970566778709No Hit
TCTGATGGGTAAGGAAAGAAGCAATCAGTAGAGAATTCAGGATAGTTTTGTT1500.10226970566778709No Hit
AAGCAAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1500.10226970566778709No Hit
GTACCAGGGGGAAGAGCTCTCGGCTGTGCAGTGGTACCGTCGGGGCCTGGGC1490.10158790763000183No Hit
GAAATCGGGGTTTCTTGAATCTTATTTGTTAATCTGACTCACAGCTGAAAAG1490.10158790763000183No Hit
AAGCATGGGATTGTGCCAAGGACTTGAATGGATGATGTCACGACTTAAGATT1490.10158790763000183No Hit
ATGAATGGGTGGGAATCTGTTTTGAACAACGGAAAACATTTTTTAAGGGGAA1490.10158790763000183No Hit
ATCGCGGGGAAGTGAAGTTCTCCTTAGAAGAGAAGATCTTTTGGGATCAGTT1490.10158790763000183No Hit
TCTTATGGGTATTCTAGCCTAGTTGATAATTTTTATTTAAAAGGTGGTCCCT1490.10158790763000183No Hit
CTCTAAGGGTTAATACAATCACTATGGAGAACAGTTTGGAAGTTCCTCAAAA1490.10158790763000183No Hit
GGATGTGGGGTATTCTGCATCAGTTTGCAGTTCACTGCAAATCTTTTACATT1480.10090610959221659No Hit
CTATGTGGGCGACTTAAGAGATGGTGAATCTGGTGCACCATGCACTTTCCTG1480.10090610959221659No Hit
TGACAGGGAAGGGATAAAATTACAAATTGAAAGTCAGGGAAGACTTATCTAA1480.10090610959221659No Hit
CCACAAGGGGAATGAAGAGCCATTTTTCTTTTCTTTTTTTTAAGTCAGTCAT1480.10090610959221659No Hit
TTATCGGGATGCATATTTGCACTTCCAGATGGGTTGCATAAGAATCAGGTCC1480.10090610959221659No Hit
TGCAGTGGGAGGTTGATATTCACCAGTATAAAAACACTCAAAATTATAAATT1470.10022431155443133No Hit
TTTAAGGGAGTGTCAGAGGAATAGTTTTTTTTCTGGCCCATATTAGTTACAG1470.10022431155443133No Hit
TTTCTCGGGGAACAGTAAAATAAATAAAAACCAATAGTACTAATTTTGCTTT1470.10022431155443133No Hit
ATAACGGGGATCTGGTCGCGGGATATGATATTCTGTAAATATTGTACAATTT1470.10022431155443133No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)