FastQCFastQC Report
Sat 31 Dec 2022
SRR5846977.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5846977.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences198368
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACGCTGAGGTATCTCGTATGC5100.25709791901919665No Hit
CTTATACACATCTCCGAGCCCACGAGACGCTGAGGTATCTCGTATGCCGTCT4240.21374415228262622TruSeq Adapter, Index 2 (95% over 21bp)
CTATTAGGGATTTGAAATTTGAATTATTCGCTGTCTTGCTGCTTAAGATGAG3010.15173818357799645No Hit
CTCAGTCGGGAACACAAGCGGGGTGCAGGTGATCTGCATGAAGGGGAAGGCC2980.15022584287788354No Hit
TTTGGCGGTGGTTGGTGCCTTCCCTATTTTTGTCCGGAATGCCATCCTAGGC2940.1482093886110663No Hit
ATGTCCGGGAATGATTGCCATTCTAACTGGTGTGAGATGGTATCTCATTGTG2820.1421600258106146No Hit
GGCGCGGGCGATGAAAAGTTAGTGGAACATTTCTGTGCAGAATTTAAAACTA2770.13963945797709307No Hit
ACTTATGGGATGAGGGCTCCGATATAAGGTGGCTGTCCTTTAATGATATATC2740.13812711727698015No Hit
TAGCCAGGAGAAGTATTTTGGTCTTTATCTAGAAGTTTGATGATTTTTTTGT2660.1340942087433457No Hit
AACTTAAGGAATGATTAATAACAGAATGTTTATTTAATGTGCTGTCCATTTT2650.1335900951766414No Hit
ACAAGGGAAACTACAAAACACTAATGAAAGAAATAAACAAATGGAAAGACAT2650.1335900951766414No Hit
AGCAAAGGGGAGAGCTACGCAGCCTTCGGTGCAGTCGTCACTCGTGTCTCGC2650.1335900951766414No Hit
ACTCCTGGGAGATTTGGGAGTCAATCAGAAATTGGCTCCTGTTTCAGAAAGT2650.1335900951766414No Hit
CTTTGCGGGTATGGGATTTTGATATTTTGGTATTCTAAAGAATTTAGTATTT2640.1330859816099371No Hit
TATATGGGGATTGTGATTGCCAGATAAAACATCAAACCAGCATATATGTAGT2560.12905307307630262No Hit
GCACTAGGGAGAGATTGGCTCCACAACTCTTGCTCTCAACCCCCCATATGAT2530.1275407323761897No Hit
GAATTGGGGGCAAATCGGCAGTATAGTATAGAGGAAAGGAGGGTGACCATTA2500.1260283916760768No Hit
TTGTGTGGGACCTGTATGACCTTGTGAAATACATTACTTATTCATTTTATAT2500.1260283916760768No Hit
GATAGTGGGGAAGGGGCAGGCGAGGCGGGGGGTGAGGAGGCTGACGAGAAGC2480.12502016454266818No Hit
AGCCTTGGGGAGGAAGTCAAATTGTCCCTGTTTGCAGATGACATGATTGTAT2470.12451605097596387No Hit
TTAACAGGGCTTGTTCCAAGGGAGCATGATTGGAAGCTTTATCGATATATAC2430.12249959670914663No Hit
CAGAGGGGTAACATTTTAATTATAGTACATCAGATTCATCTTTGTATCCCCA2420.12199548314244234No Hit
TAACCTGGGCTAGCGCGTCCAGGTGGGAGGTTAGTGTGGCCCGGGCGTCCGC2420.12199548314244234No Hit
AGAAGCGGGGGGAGAGGGTAATGTTACTTCACAAAGGACATGTCAGATCCTT2410.12149136957573803No Hit
ACATAGGGGCAGGAGAAGTATACCAAGCCAAATGGTGACAAGGAACTCTCTG2410.12149136957573803No Hit
TAGTAAGGGATCTGATTCTGGCAGAGAGGGCTCCTCCATCTGAAACGCATGG2390.1204831424423294No Hit
GCCATGGGGATGAATAGAACATTACCCCATATCTCTATTAACATTGAATGTA2380.1199790288756251No Hit
TCAACAGGGAAGGCAGAGCAGACTACTGATCTTAATAAAAGGAAAATTATTT2380.1199790288756251No Hit
CGTTATGGGCTGGGCATAAATCTTAATAAAACTTAATCTCAGAAAAGTTTGT2380.1199790288756251No Hit
GCTCGGGAACAAACATTTCATTCCTTAATGTTTTAAGTTTATTTTAGTAGTT2370.11947491530892079No Hit
CTTATCGGGGTCTGTGGTAATTCTCAAAACAAAATTTCACTTCTGTTTTCCA2360.11897080174221648No Hit
AACGTAGGGGTGCATCATAAAGGAATATTCTCGTTCTCTTTACTAATCTATT2360.11897080174221648No Hit
GACCTCAGGTGATTCAGCCACCTCGGCCTCCCAAAGTGCTGGGATTATAGGC2350.11846668817551219No Hit
GTGCTGAAGGGGATAAATGATTCTTTTTATGCAGACTAGCAAATGAAATAGG2350.11846668817551219No Hit
TTGGCTGGGGACATTACAAGAGTATAAAAATGGACATTAAATCATGGCCTTG2340.11796257460880788No Hit
GCAGCTGGGCTTCCGGTCGCGGGACACCGGGTGTAGAGGGCGGTCGCGGCGG2330.11745846104210356No Hit
GACCGAGGGGAATTAGTGGAGCTTTTGTATCTTCTGCAGACACTGTGGGTAG2330.11745846104210356No Hit
CGCTACGGGTGGAAAAATAAATTATATTTGCCTCTGGAAAGGTCTTACGTGA2330.11745846104210356No Hit
GCCATGGGGATTTCTAAAGATGAAGTAACAGGGGAAGTGATGAACTTTATAA2320.11695434747539925No Hit
TTAATAGGGATGCACTTTCCTGCTAGACTCTGGCCTAGTTCAAGCTGAAATG2320.11695434747539925No Hit
CCGTCGGGTCATGATAACAACTCTCAGTAAACTAACAGTAGAAGCAAACTTC2320.11695434747539925No Hit
CTATGAGGGCTTGCTTCTTGTGTTTGAAACACTCGTGTGCATGAGAATGTCA2310.11645023390869495No Hit
TCGCAAGGGACCTTTCTCAGATGAATTAAATGATTTTAATAACTTCCCAATT2310.11645023390869495No Hit
TCCGCAGGGCATAGGGAACAGCATAAGAAAAAGAAATGAAGGAGACAGACAG2310.11645023390869495No Hit
AAATGGGAAATCAAGACAGTTCTTTCCAAGTCACTGAAAATTTAATGTCAGA2300.11594612034199064No Hit
TGCAAAGGGAAGAAAGTCATGCTGAATCTGAGAATGCCAGGACATTTCACGT2300.11594612034199064No Hit
ATTACGGGGCAATAAAAGATATTAAGAATTGTTATCCTTAACTTACGGGCTT2300.11594612034199064No Hit
AGCTGTGGGAGAAATTTAAGAGTTGCTTATATTTGGAACCACTGTAGTACTT2280.11493789320858204No Hit
GCTTACGGGGCGCGGCGCGGCCCCCGAGCGCACCGGGCCTGCCGAGGAGCGC2270.11443377964187773No Hit
ATTATCGGGCAAATGAGAAAGTGAGACACAGAGATGCTAAGGAATTGCCCAA2260.11392966607517341No Hit
CGTAACGGGGCATGGTGAAACCCCGTCTCTACTAAAAATATAGAAAAATTAG2260.11392966607517341No Hit
GGGCTGGGCACTTGCCAAAATAATACATACTTCATAGACCACTGAGTTCTAG2260.11392966607517341No Hit
GTGTTGGGGGTCAAGTGAATGTATTTTGAAAATCTTCTCTTCTGTCTGTCTT2260.11392966607517341No Hit
AACACCGGGGGGTCTCCGCATCCCTCTGTTCCCTGTCCCAGCAGAGGCTGTG2260.11392966607517341No Hit
GTCAAGGGGTTTCTATGAAAGAGACGGTTACTGATATTGATAGGTAGAATGT2260.11392966607517341No Hit
CATGCAGGGAACAGAGTTAAAGAATTAAGAAGGGAAAATGAAGATACTGAAC2260.11392966607517341No Hit
AAAGCTGGGGGAAATAGCAATATTAATTGAAGCTTACAAGGGGCTGCCCAAA2250.1134255525084691No Hit
AATCTAGGGGGAGTCAAACACTGGATGCAGAAATTTTGGATTTTTCATCACT2250.1134255525084691No Hit
ACATCGGGTGTAAAGATACAGGTACAAGCATGTTTATTGCAGTATTGTTCTT2240.1129214389417648No Hit
GTTATTGGGCAACTGTTTATGTGTTTGGATTTTCACTAATTACTCCTCTCCA2240.1129214389417648No Hit
CTTGTTGAGGGCACCAATTTGAAAGGTATAATTACTCATGTTGATGTGAATA2240.1129214389417648No Hit
GAGTGCTGGGGTGTATTAGAGATGTAGCCAAAATACAAACAGATAAGTAAAA2230.1124173253750605No Hit
CAAGTAGGGTGTCAAATCGAAGCTGATTTGAAGTTAAAGGAATAAGGTATAG2220.11191321180835619No Hit
GCTTGTGGGATGGTACACTCAACCAATCTCAAAACGAATCCAAGGGCACCAG2220.11191321180835619No Hit
ACTGCTGGGGTAGAAACAATTTTACATCTACCTGAAGAAAATGTCAACACAG2220.11191321180835619No Hit
TATAAGGGGGCCTTTCTCCTTTCAGTATTTCAGTGTGTTTATGGTTGCTGCT2200.11090498467494757No Hit
GCGCGTGGGGCTCTTCAGCAGAAAATCTTTGTGGAACACATTCCACACTTTG2200.11090498467494757No Hit
ATGAAAGGGGACAGGATTTGTTTATTTTCTAAAATTAGCTTCATTCAATATT2200.11090498467494757No Hit
CATTTTGGGGCACCTACACCCGCCACGGCTACATCTTTTCGTCGCTTGCCGG2190.11040087110824326No Hit
TACATGGGGCACGTATCTCCTTTATAAATGTTTTTGTAAATTATTTTGCTAA2190.11040087110824326No Hit
TGGTTCGGGGGGGGAGCAGGAAGAGGGGCAGAAGGAAGAAGAAGAGGGGGAG2180.10989675754153895No Hit
CGTTACGGGGTTCCACCAAAACACGTTAGAGAATTTGTACATTAGAGAATTT2170.10939264397483466No Hit
GTCTGTCGGGAACCCTCTGAAGAGAAGACTTCTGAAAGCAAAAAGACTTACA2170.10939264397483466No Hit
ATGCAGGGCAGAGGGAGGCAGTGGGTGAGAGGAGCTTCCTTTCATTTTTGAA2170.10939264397483466No Hit
CTCGTCCTGGGTGAAGAAAGGGAGAAGATGTTTGAGTTCTACGAGCGAGTGT2160.10888853040813035No Hit
TCAGCTGGGATTAAATGAGTGCTATCTGTGAAATTGGTTATATTAGGTGGCT2160.10888853040813035No Hit
CAATTGCGGGAGGCTATCAGAGCTGGGTCACAATTTAGTGGAGAAGATGAGT2160.10888853040813035No Hit
CTACTTGGGTCACAAAAAACTTCCAATGCCATTTTTGAGAACTAACCTAACT2150.10838441684142605No Hit
ACACATGGGGTAAGCATCAAGCAGCCTGTCAGGAAAAGGATGAACAAAAAAG2150.10838441684142605No Hit
ATGGTTGGGATATCTAAGTTCAAATAGATAATATTTTAATGAGAAAATACCA2150.10838441684142605No Hit
TAAAAGGGTACCATATCCACTGAAAATATCTTCCAGGAATGAAGGTGAAATA2150.10838441684142605No Hit
TAATCAGGGGATGCTGATGCATTTTGATGCTTTGTGCATTCATTTCTTTCTG2140.10788030327472171No Hit
CTTCGTGGGGTATCTTCAAAGCCAACAACAGTGGGTCAAGTGCCAAATTCTT2140.10788030327472171No Hit
CTGCTGGGGTAGAGAGCTTCAAATAGTTTCTATGTGCCATAATCACTCATTT2140.10788030327472171No Hit
GCTCTTGGGGAGGTAAGATTGTTCTGTCAGAAAACCCTCTCCCAGTTTCCCC2130.10737618970801742No Hit
CAAACGGGGAACATACATTGCTTGGCCAAAGCCATCAACCAGATTGCTGCAG2130.10737618970801742No Hit
CCATCCGGGCGATCTCCCTGCGGGGAGACGTTCTTCGCCGAGAGTCGTCGGG2120.10687207614131311No Hit
AGGACTGGGAGTTCAGCAGGAAGTATTGTCTGTGTGTGATGACGGGGCAGTA2120.10687207614131311No Hit
TACTGCGGGTGTTCAAAAAACCACAGCACAAAAAATAAACTTGTTTTATTCT2110.1063679625746088No Hit
CGAACAGGGACCGAAAAATTGCAGAAGCACAAGCCAAACTGGCCGAAGAACA2100.1058638490079045No Hit
TCGACCGGGCGATCTGAGATGGGGTGCCAAAAAATTTTAAGCAAATGAAAGC2100.1058638490079045No Hit
CACCTGGGGAGGGAAACAAAATGTGGTCATTCATCAGTCACTTAGTCATTGA2090.1053597354412002No Hit
GATCTTGGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCC2090.1053597354412002No Hit
TCTAGAGGGTATGATTCTTGAGGTAACTCTACCAGATACTTCCAGATTTAGA2090.1053597354412002No Hit
GTCAACGGGCTTGTGGTTTAGGTTTAAAACCTAGAATAAAAGACAGAAAGCT2090.1053597354412002No Hit
ATTATTGGGTAGAATTGTATTCAAATAAAATATAAAAAAGAAAGATCACTTA2090.1053597354412002No Hit
CCACTGGGGAATAGACAAGTCCACTATTATAGTTTCAGACTTCAACACTCTT2090.1053597354412002No Hit
CTTAAAGGGACTTTTGTATTTGGGAGAATAGATTCCTGTGTAGCAGTGGGGA2090.1053597354412002No Hit
ATCAAAGGGTGGCTAATACCTATTCTTCAACCACCTCGGTTACTCTGACATA2080.1048556218744959No Hit
AATTGCGGGATTGTGAGAGGGACTCTTTGCAATTTCTCTGATTTCTTTGTAT2070.10435150830779157No Hit
CAAACAGGACTTATTCTGCATTCAGAAAATATACTCAACTAGAACCATTCAG2070.10435150830779157No Hit
CTCTCCGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC2070.10435150830779157No Hit
TCGACTGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC2070.10435150830779157No Hit
AACGCCGGGATCAACAGAAAGCGCTACTGCTCCATCTCACTAGTAGTCAGGG2070.10435150830779157No Hit
CACAATGGGTCCAAGGCCTAATAGGAGTGCAGCAGCCACCTCAGAGTCAGAA2070.10435150830779157No Hit
TTTTGAGGGATCTTGTTTTCTAATCTATTCACTTGCCCAGGTCAGAAATCTA2060.10384739474108727No Hit
CACTTTGGGTGTTCTATGTGTATTTAAACTGGGAGGTCTGAACTAAATGATC2050.10334328117438296No Hit
GCTGCAGGGGTGAGACTAAGAATGTCTGACACCTGCCTTCCTCACTTCCCAA2050.10334328117438296No Hit
TTTCACGGGAGTTAAATCATATAATGTTTGTATGATTAAAAATAAATTTCTC2040.10283916760767867No Hit
ACAAGGGGGGTTTGTAAGTACCAATCAATAAATGTGATCATTTACTAATGGC2040.10283916760767867No Hit
AAGAGTGGGTATAGAGCTTTGTCTCCCACTTTGTGCTGTTTTTCTCTTACAG2040.10283916760767867No Hit
TTTAAAGGGACGTGCTTCCGCTGGAGCCTCTCGGGAGGCGGGTAACGTTATA2040.10283916760767867No Hit
ATAATAGGGCTGTCGAGGGACCCAACCAAAAACAAACCTGAGACAGTGGTAT2040.10283916760767867No Hit
TGGCATGGGGGTGTAATTTATATGAGAGTTTCATTTATAACAACGTTACCTG2030.10233505404097436No Hit
GACAAAGGGATGGGCCAGCCCCCTCCATCTGTACTTGGTCCAACTGCAACTC2030.10233505404097436No Hit
ATTGGAGGGATTCTCTAGGAACTCACTTTTGAGAGTATTTTTCTCTAACCCA2030.10233505404097436No Hit
GCCAATGGGGCTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAA2030.10233505404097436No Hit
TGCACAGGGACCAGGCTTTGGAAATCCTTAATAATAAGGAGCAAATTTCCAG2020.10183094047427005No Hit
GGACGGGACCATCAGGAGAAGGAGTTTGAGACTGTGTATGCAACCCCCAATA2020.10183094047427005No Hit
GGTCGGGGCCAGTCAGGTGGGGCAGGGACCTGGGGGACCGGGCTGGGAGGCG2010.10132682690756573No Hit
AAAACCGGGGTGTGGGTCAATGATTCTCATACTGTTATACTTGTGTACTGGA2010.10132682690756573No Hit
CTTTCAGGGGAAATGATGAGATGAGATAAAATGAGATGAAATGATGAGATGA2010.10132682690756573No Hit
ATTAATAGGGACCACCATCACTACCACCACCACCACCATCACCACCACCACC2010.10132682690756573No Hit
TGACTAGGGCACTTGACTCAGTGGTTTCACAGGGTCTCACCCTCACCTTCTA2000.10082271334086142No Hit
GCATAGGGGTTACAAGTAATCTGACACTAGGTGCCACATAATTAAGATAAAG2000.10082271334086142No Hit
GCTTAAGGGGACGCTCTTCCGATCTGCGTTTGGGCAGCACTGTGCTACAAAA2000.10082271334086142No Hit
TCTACCGGGAAGGGATTACACCAAATATACAACTGGAGGATTTTCCTACATC2000.10082271334086142No Hit
CTTCGCGGGAGAACAACCTAAGAAACCCTTTGTGTCCTGAGGGGTCGTTGAC2000.10082271334086142No Hit
ATCGCCGGGGTGAAGGCAAGGGCCATGTCTATCTTATTCGTCATTAGATTCC2000.10082271334086142No Hit
ATCTGGGGGACACTGCCCTTGGCAGAAAAGTTTTCTAAAATATAATAATAAA2000.10082271334086142No Hit
TGCAACGGATACCATCACCTGTTCATACTCATTTAATTCTAATATAGTTATA1990.10031859977415712No Hit
AGCCCAGGGCTGTGCCTCTACTTCCCCATCTGTAAAATGGAGTTAATAATCA1990.10031859977415712No Hit
TGCAATGGGAGTTGAATTGAATGTCTATAAGGTCTAAAGGTAGAATGTGAAT1990.10031859977415712No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)