FastQCFastQC Report
Sat 31 Dec 2022
SRR5846976.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5846976.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences197545
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACGCTGAGGTATCTCGTATGC4780.24197018400870687No Hit
CTTATACACATCTCCGAGCCCACGAGACGCTGAGGTATCTCGTATGCCGTCT3950.1999544407603331TruSeq Adapter, Index 2 (95% over 21bp)
GCAGCTGGGCTTCCGGTCGCGGGACACCGGGTGTAGAGGGCGGTCGCGGCGG2780.14072742919334835No Hit
GGCGCGGGCGATGAAAAGTTAGTGGAACATTTCTGTGCAGAATTTAAAACTA2760.13971500164519476No Hit
TCTGGGGCAATCCGTTGATCCAGAAATTATAGCTCGAGGTACTGTTGGCTTT2720.1376901465488876No Hit
ATGTCCGGGAATGATTGCCATTCTAACTGGTGTGAGATGGTATCTCATTGTG2690.13617150522665722No Hit
GCACTAGGGAGAGATTGGCTCCACAACTCTTGCTCTCAACCCCCCATATGAT2670.13515907767850363No Hit
AGCAAAGGGGAGAGCTACGCAGCCTTCGGTGCAGTCGTCACTCGTGTCTCGC2660.13465286390442682No Hit
TCCGCAGGGCATAGGGAACAGCATAAGAAAAAGAAATGAAGGAGACAGACAG2660.13465286390442682No Hit
CTATTAGGGATTTGAAATTTGAATTATTCGCTGTCTTGCTGCTTAAGATGAG2650.13414665013035004No Hit
TCAACAGGGAAGGCAGAGCAGACTACTGATCTTAATAAAAGGAAAATTATTT2630.13313422258219645No Hit
CTCAGTCGGGAACACAAGCGGGGTGCAGGTGATCTGCATGAAGGGGAAGGCC2610.13212179503404287No Hit
GATAGTGGGGAAGGGGCAGGCGAGGCGGGGGGTGAGGAGGCTGACGAGAAGC2560.1295907261636589No Hit
CTATTGGGGATTTTTGGGCAGGTATGTACTGGCAGTAAGTTGAAAACGTTAG2560.1295907261636589No Hit
ATTACGGGGCAATAAAAGATATTAAGAATTGTTATCCTTAACTTACGGGCTT2550.12908451238958213No Hit
AACTTAAGGAATGATTAATAACAGAATGTTTATTTAATGTGCTGTCCATTTT2540.12857829861550532No Hit
ACATCGGGTGTAAAGATACAGGTACAAGCATGTTTATTGCAGTATTGTTCTT2520.12756587106735176No Hit
GACCGAGGGGAATTAGTGGAGCTTTTGTATCTTCTGCAGACACTGTGGGTAG2510.12705965729327495No Hit
GCTTACGGGGCGCGGCGCGGCCCCCGAGCGCACCGGGCCTGCCGAGGAGCGC2510.12705965729327495No Hit
TTTGGCGGTGGTTGGTGCCTTCCCTATTTTTGTCCGGAATGCCATCCTAGGC2490.12604722974512136No Hit
TATAAGGGGGCCTTTCTCCTTTCAGTATTTCAGTGTGTTTATGGTTGCTGCT2480.12554101597104458No Hit
CTTATCGGGGTCTGTGGTAATTCTCAAAACAAAATTTCACTTCTGTTTTCCA2470.12503480219696778No Hit
TGCAAAGGGAAGAAAGTCATGCTGAATCTGAGAATGCCAGGACATTTCACGT2470.12503480219696778No Hit
ACTTATGGGATGAGGGCTCCGATATAAGGTGGCTGTCCTTTAATGATATATC2470.12503480219696778No Hit
AAAGCTGGGGGAAATAGCAATATTAATTGAAGCTTACAAGGGGCTGCCCAAA2450.12402237464881419No Hit
GCCATGGGGATTTCTAAAGATGAAGTAACAGGGGAAGTGATGAACTTTATAA2450.12402237464881419No Hit
CACTTTGGGTGTTCTATGTGTATTTAAACTGGGAGGTCTGAACTAAATGATC2430.1230099471006606No Hit
TTTTGAGGGATCTTGTTTTCTAATCTATTCACTTGCCCAGGTCAGAAATCTA2400.12149130577843023No Hit
TCTAGAGGGTATGATTCTTGAGGTAACTCTACCAGATACTTCCAGATTTAGA2390.12098509200435344No Hit
TCGACCGGGCGATCTGAGATGGGGTGCCAAAAAATTTTAAGCAAATGAAAGC2390.12098509200435344No Hit
GCTGCAGGGGTGAGACTAAGAATGTCTGACACCTGCCTTCCTCACTTCCCAA2380.12047887823027664No Hit
CAAGTAGGGTGTCAAATCGAAGCTGATTTGAAGTTAAAGGAATAAGGTATAG2370.11997266445619985No Hit
GAATTGGGGGCAAATCGGCAGTATAGTATAGAGGAAAGGAGGGTGACCATTA2370.11997266445619985No Hit
TAGCCAGGAGAAGTATTTTGGTCTTTATCTAGAAGTTTGATGATTTTTTTGT2360.11946645068212305No Hit
ACTCCTGGGAGATTTGGGAGTCAATCAGAAATTGGCTCCTGTTTCAGAAAGT2360.11946645068212305No Hit
GTTATTGGGCAACTGTTTATGTGTTTGGATTTTCACTAATTACTCCTCTCCA2340.11845402313396948No Hit
TACATGGGGCACGTATCTCCTTTATAAATGTTTTTGTAAATTATTTTGCTAA2340.11845402313396948No Hit
TAAAAGGGTACCATATCCACTGAAAATATCTTCCAGGAATGAAGGTGAAATA2340.11845402313396948No Hit
GCCATGGGGATGAATAGAACATTACCCCATATCTCTATTAACATTGAATGTA2330.11794780935989269No Hit
TTTGTAGGGATTCTCTGCTAGTGGAGATGCAAACTGACACAGCTCTTCTGGA2330.11794780935989269No Hit
CGTTATGGGCTGGGCATAAATCTTAATAAAACTTAATCTCAGAAAAGTTTGT2330.11794780935989269No Hit
AGCTGTGGGAGAAATTTAAGAGTTGCTTATATTTGGAACCACTGTAGTACTT2310.1169353818117391No Hit
TTAATAGGGATGCACTTTCCTGCTAGACTCTGGCCTAGTTCAAGCTGAAATG2300.11642916803766232No Hit
GCTCTTGGGGAGGTAAGATTGTTCTGTCAGAAAACCCTCTCCCAGTTTCCCC2280.11541674048950873No Hit
CTGCTGGGGTAGAGAGCTTCAAATAGTTTCTATGTGCCATAATCACTCATTT2280.11541674048950873No Hit
CAATTGCGGGAGGCTATCAGAGCTGGGTCACAATTTAGTGGAGAAGATGAGT2280.11541674048950873No Hit
GCTCGGGAACAAACATTTCATTCCTTAATGTTTTAAGTTTATTTTAGTAGTT2270.11491052671543193No Hit
ATTATTGGGTAGAATTGTATTCAAATAAAATATAAAAAAGAAAGATCACTTA2270.11491052671543193No Hit
TCGACAGGGAACACAGTGAAGGTAGATGACACTTTCAAGAGCTTTGTGAATT2270.11491052671543193No Hit
AATGCAGGGAGTCCACCCCTTGGAGCTAGACATCCTGTACTTAGTCACGGGG2260.11440431294135513No Hit
AATCTAGGGGGAGTCAAACACTGGATGCAGAAATTTTGGATTTTTCATCACT2260.11440431294135513No Hit
CGCTACGGGTGGAAAAATAAATTATATTTGCCTCTGGAAAGGTCTTACGTGA2260.11440431294135513No Hit
ACAAGGGGGGTTTGTAAGTACCAATCAATAAATGTGATCATTTACTAATGGC2250.11389809916727833No Hit
TCATCAGGGAAATGATGATATTTCAGAACTGGAAGACCTTTCGGAATTGGAA2240.11339188539320154No Hit
TTAAACGGGATTGGTTTTTAGTATATTCACAGAATTCTACAACCATCACTAT2240.11339188539320154No Hit
CTTAAAGGGACTTTTGTATTTGGGAGAATAGATTCCTGTGTAGCAGTGGGGA2240.11339188539320154No Hit
CACAATGGGTCCAAGGCCTAATAGGAGTGCAGCAGCCACCTCAGAGTCAGAA2230.11288567161912474No Hit
CAGCTGAGATTGTACCACTGCACTCCTGCCTGAGTGAAAGAGTGAGACCGTG2230.11288567161912474No Hit
TGCACAGGGACCAGGCTTTGGAAATCCTTAATAATAAGGAGCAAATTTCCAG2220.11237945784504796No Hit
GACCTCAGGTGATTCAGCCACCTCGGCCTCCCAAAGTGCTGGGATTATAGGC2220.11237945784504796No Hit
CGTTACGGGGTTCCACCAAAACACGTTAGAGAATTTGTACATTAGAGAATTT2210.11187324407097117No Hit
CTCGTCCTGGGTGAAGAAAGGGAGAAGATGTTTGAGTTCTACGAGCGAGTGT2210.11187324407097117No Hit
CTATGAGGGCTTGCTTCTTGTGTTTGAAACACTCGTGTGCATGAGAATGTCA2210.11187324407097117No Hit
ACATAGGGGCAGGAGAAGTATACCAAGCCAAATGGTGACAAGGAACTCTCTG2210.11187324407097117No Hit
CATTTTGGGGCACCTACACCCGCCACGGCTACATCTTTTCGTCGCTTGCCGG2200.11136703029689438No Hit
CAGAGGGGTAACATTTTAATTATAGTACATCAGATTCATCTTTGTATCCCCA2200.11136703029689438No Hit
TAACCTGGGCTAGCGCGTCCAGGTGGGAGGTTAGTGTGGCCCGGGCGTCCGC2200.11136703029689438No Hit
CAGCTAGGGCAGGTTATTTCATGACCCAGATACTAAGCCTACTACCCAATAG2200.11136703029689438No Hit
GTGCTGAAGGGGATAAATGATTCTTTTTATGCAGACTAGCAAATGAAATAGG2200.11136703029689438No Hit
TTAACAGGGCTTGTTCCAAGGGAGCATGATTGGAAGCTTTATCGATATATAC2190.11086081652281758No Hit
CTTGTTGAGGGCACCAATTTGAAAGGTATAATTACTCATGTTGATGTGAATA2190.11086081652281758No Hit
ATGGTTGGGATATCTAAGTTCAAATAGATAATATTTTAATGAGAAAATACCA2190.11086081652281758No Hit
TCCTAAGGGTATTGATACAAAAAAAGCCTCCACGAATGTGTTTCTGTTACCA2190.11086081652281758No Hit
AAATGGGAAATCAAGACAGTTCTTTCCAAGTCACTGAAAATTTAATGTCAGA2170.10984838897466401No Hit
AGCACAGGGATTGAAAAAAATGCAGGTGACCTAGCTGAAACAGAGAAGAGTA2170.10984838897466401No Hit
TAGCCAGGGGAGAGGCAGAGGCAGCATCCAGCGGCGGCGCCAGCAGTTCCAG2160.10934217520058721No Hit
TTGTGTGGGACCTGTATGACCTTGTGAAATACATTACTTATTCATTTTATAT2160.10934217520058721No Hit
CGTAACGGGGCATGGTGAAACCCCGTCTCTACTAAAAATATAGAAAAATTAG2150.10883596142651042No Hit
TAGTAAGGGATCTGATTCTGGCAGAGAGGGCTCCTCCATCTGAAACGCATGG2140.10832974765243363No Hit
CTACTTGGGTCACAAAAAACTTCCAATGCCATTTTTGAGAACTAACCTAACT2140.10832974765243363No Hit
AGGACTGGGAGTTCAGCAGGAAGTATTGTCTGTGTGTGATGACGGGGCAGTA2140.10832974765243363No Hit
GTACACGGGGCCTCAATCCACACTGACTTATGGAAAAAATTTACTGGGTTGC2140.10832974765243363No Hit
TTGGCTGGGGACATTACAAGAGTATAAAAATGGACATTAAATCATGGCCTTG2140.10832974765243363No Hit
TGGCATGGGGGTGTAATTTATATGAGAGTTTCATTTATAACAACGTTACCTG2130.10782353387835683No Hit
CTCTCCGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC2130.10782353387835683No Hit
CTTTCTGGGAATCTTCATGTTCTCATTTCAACAATGTTCACAGCATCTTCAC2120.10731732010428004No Hit
GTCAACGGGCTTGTGGTTTAGGTTTAAAACCTAGAATAAAAGACAGAAAGCT2120.10731732010428004No Hit
GGGCTGGGCACTTGCCAAAATAATACATACTTCATAGACCACTGAGTTCTAG2120.10731732010428004No Hit
GACAGCACCTGAAATTATCCTGCCAGGAGCCTTCTGTTACTAATCATAAGCA2120.10731732010428004No Hit
TTTCACGGGAGTTAAATCATATAATGTTTGTATGATTAAAAATAAATTTCTC2120.10731732010428004No Hit
TATATGGGGATTGTGATTGCCAGATAAAACATCAAACCAGCATATATGTAGT2120.10731732010428004No Hit
GAGTGCTGGGGTGTATTAGAGATGTAGCCAAAATACAAACAGATAAGTAAAA2120.10731732010428004No Hit
AGAACGGTATGGTAATTTAATTATTTTGCCACAATATGAAGTTTCTTATGGA2110.10681110633020326No Hit
TCGCAAGGGACCTTTCTCAGATGAATTAAATGATTTTAATAACTTCCCAATT2110.10681110633020326No Hit
CAAACAGGACTTATTCTGCATTCAGAAAATATACTCAACTAGAACCATTCAG2100.10630489255612646No Hit
ACAAGGGAAACTACAAAACACTAATGAAAGAAATAAACAAATGGAAAGACAT2100.10630489255612646No Hit
CCTCTTGGGAACGCCTTCAGAACGATTTGAAGGTCCATATACAGACTTCACC2100.10630489255612646No Hit
ATTATCGGGCAAATGAGAAAGTGAGACACAGAGATGCTAAGGAATTGCCCAA2100.10630489255612646No Hit
TTTAGAGGGGAAGTATCTCTTGGAGTTTCAGAAATGCTAGCTTGGACAACTG2100.10630489255612646No Hit
ATCAAAGGGTGGCTAATACCTATTCTTCAACCACCTCGGTTACTCTGACATA2100.10630489255612646No Hit
GCCAATGGGGCTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAA2100.10630489255612646No Hit
GCATAGGGGTTACAAGTAATCTGACACTAGGTGCCACATAATTAAGATAAAG2090.10579867878204967No Hit
ATGAAAGGGGACAGGATTTGTTTATTTTCTAAAATTAGCTTCATTCAATATT2090.10579867878204967No Hit
TTTAAAGGGACGTGCTTCCGCTGGAGCCTCTCGGGAGGCGGGTAACGTTATA2090.10579867878204967No Hit
GTCAAGGGGTTTCTATGAAAGAGACGGTTACTGATATTGATAGGTAGAATGT2080.10529246500797287No Hit
TAGTGTGGGAGGAAAACTTAGAACTTTGTGTGAAATAGACTGGCCAGCATTA2070.10478625123389607No Hit
ACTGCTGGGGTAGAAACAATTTTACATCTACCTGAAGAAAATGTCAACACAG2070.10478625123389607No Hit
AACGTAGGGGTGCATCATAAAGGAATATTCTCGTTCTCTTTACTAATCTATT2070.10478625123389607No Hit
TTTGATGGGGGGGATTCCAAAAGGGGCTGCAGATTCTAGACTGAAGATAATG2060.10428003745981927No Hit
TAATCAGGGGATGCTGATGCATTTTGATGCTTTGTGCATTCATTTCTTTCTG2060.10428003745981927No Hit
GCTTAAGGGGACGCTCTTCCGATCTGCGTTTGGGCAGCACTGTGCTACAAAA2060.10428003745981927No Hit
ACACATGGGATTATCTTTTGCATTAACCTATTTTTTTCCATCATACATGCTA2060.10428003745981927No Hit
AATTGCGGGATTGTGAGAGGGACTCTTTGCAATTTCTCTGATTTCTTTGTAT2050.10377382368574248No Hit
CGAACAGGGACCGAAAAATTGCAGAAGCACAAGCCAAACTGGCCGAAGAACA2050.10377382368574248No Hit
GAACTTGGGGCCGACCTGCAGCGGGTCATCGCCAACTGTCGCGAGTACAACC2050.10377382368574248No Hit
GCTTATGGGCTCTTGAAAAACACTCCTAGTATCAGCGAGGAACAAATTAAGG2050.10377382368574248No Hit
GACACAGGGGAATTGCCTCGGTGATGTCGTGGGTTCAAGCAGCCTCCTTGAT2040.10326760991166568No Hit
CACCTGGGGAGGGAAACAAAATGTGGTCATTCATCAGTCACTTAGTCATTGA2030.1027613961375889No Hit
GACAAAGGGATGGGCCAGCCCCCTCCATCTGTACTTGGTCCAACTGCAACTC2030.1027613961375889No Hit
GGTCGGGGCCAGTCAGGTGGGGCAGGGACCTGGGGGACCGGGCTGGGAGGCG2030.1027613961375889No Hit
ATCTGGGGGACACTGCCCTTGGCAGAAAAGTTTTCTAAAATATAATAATAAA2030.1027613961375889No Hit
TGAAATGGGGAGGGCCTCATGTTACGGCATTGCCTTTTCTTTCTGTGGATCC2030.1027613961375889No Hit
CTGCAAGGGGTGGCTGAATAGTAAGATGAATGCACAGAACAAACTAAGTCTC2020.10225518236351211No Hit
GCTTGTGGGATGGTACACTCAACCAATCTCAAAACGAATCCAAGGGCACCAG2020.10225518236351211No Hit
TTCGACGGGATTGAAATTATACAGTTGTTGTCTTTACTGTCTGCCTTCTTTC2020.10225518236351211No Hit
AATGTGGGGGCAGTTCATACTGAATGACCACTTGATATGACTAATTCAGGGA2020.10225518236351211No Hit
CCGTCGGGTCATGATAACAACTCTCAGTAAACTAACAGTAGAAGCAAACTTC2020.10225518236351211No Hit
TGTGAAGGGCAAAAGGTATTGTAAATATGATAGTAGGCAAACACGTACACAA2020.10225518236351211No Hit
TTTAGTGGGATCTCAGGGTGTTTCTGTTGCTTGATTTTTCTTCCTTTTTTCT2020.10225518236351211No Hit
TGCTGCGGGTACCAAGTTAATCTAATTGTAGTTGTTGATAATTTTTTTTCTT2010.10174896858943532No Hit
ATCTTTGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC2010.10174896858943532No Hit
CAACTGGGCTCAGCAGTGCAGTTGTGCTTCAGTCTGCAAGTCTAGCTAGCTT2000.10124275481535852No Hit
ATTTAAGGGGCCCTACAGAGGTGCCAGCTATTTCTGTTGATACTATGTATAA2000.10124275481535852No Hit
GTGTTGGGGGTCAAGTGAATGTATTTTGAAAATCTTCTCTTCTGTCTGTCTT2000.10124275481535852No Hit
AAGCCTGGGATGTTAACCCTTGGTATTTAGAAAATTAAAACCTTATAATATT2000.10124275481535852No Hit
TTCATAGGGGGAAGTAAATGTGATTATATTTGTGGAGCATCTAGCATAATGG1990.10073654104128173No Hit
CATGCAGGGAACAGAGTTAAAGAATTAAGAAGGGAAAATGAAGATACTGAAC1990.10073654104128173No Hit
TGATTGGGGCACATTTCATGTCTTTGAAAGTGTTGTACATTACTGTAAACCA1980.10023032726720493No Hit
ATTGATGGGGCTCTGGACTGGCGCAGGCGCAAGCCGGCAAGATGGCGGCGGC1980.10023032726720493No Hit
CTTTGCGGGAGAAGAAAACACCTCTTAACACCAGCTTAACACCTAGAGTGAG1980.10023032726720493No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)