FastQCFastQC Report
Sat 31 Dec 2022
SRR5846975.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5846975.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences194615
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACGCTGAGGTATCTCGTATGC4300.22094905325899852No Hit
CTTATACACATCTCCGAGCCCACGAGACGCTGAGGTATCTCGTATGCCGTCT4090.21015851809983813TruSeq Adapter, Index 2 (95% over 21bp)
AACTTAAGGAATGATTAATAACAGAATGTTTATTTAATGTGCTGTCCATTTT3020.15517817228887804No Hit
GGCGCGGGCGATGAAAAGTTAGTGGAACATTTCTGTGCAGAATTTAAAACTA2750.14130462708424324No Hit
CGTTATGGGCTGGGCATAAATCTTAATAAAACTTAATCTCAGAAAAGTTTGT2650.1361662770084526No Hit
CATTTTGGGGCACCTACACCCGCCACGGCTACATCTTTTCGTCGCTTGCCGG2640.13565244200087354No Hit
ACTCCTGGGAGATTTGGGAGTCAATCAGAAATTGGCTCCTGTTTCAGAAAGT2610.1341109369781363No Hit
GCACTAGGGAGAGATTGGCTCCACAACTCTTGCTCTCAACCCCCCATATGAT2610.1341109369781363No Hit
TTTGGCGGTGGTTGGTGCCTTCCCTATTTTTGTCCGGAATGCCATCCTAGGC2600.13359710197055724No Hit
CTCAGTCGGGAACACAAGCGGGGTGCAGGTGATCTGCATGAAGGGGAAGGCC2580.1325694319553991No Hit
TAGCCAGGAGAAGTATTTTGGTCTTTATCTAGAAGTTTGATGATTTTTTTGT2550.13102792693266194No Hit
TCCGCAGGGCATAGGGAACAGCATAAGAAAAAGAAATGAAGGAGACAGACAG2550.13102792693266194No Hit
CTATTAGGGATTTGAAATTTGAATTATTCGCTGTCTTGCTGCTTAAGATGAG2540.13051409192508287No Hit
AGCAAAGGGGAGAGCTACGCAGCCTTCGGTGCAGTCGTCACTCGTGTCTCGC2540.13051409192508287No Hit
GTTATTGGGCAACTGTTTATGTGTTTGGATTTTCACTAATTACTCCTCTCCA2530.13000025691750378No Hit
TTAATAGGGATGCACTTTCCTGCTAGACTCTGGCCTAGTTCAAGCTGAAATG2500.12845875189476658No Hit
ACAAGGGGGGTTTGTAAGTACCAATCAATAAATGTGATCATTTACTAATGGC2460.12640341186445034No Hit
GCTCGGGAACAAACATTTCATTCCTTAATGTTTTAAGTTTATTTTAGTAGTT2450.12588957685687127No Hit
TTTCACGGGAGTTAAATCATATAATGTTTGTATGATTAAAAATAAATTTCTC2440.12537574184929218No Hit
CTCGTCCTGGGTGAAGAAAGGGAGAAGATGTTTGAGTTCTACGAGCGAGTGT2430.12486190684171312No Hit
TACATGGGGCACGTATCTCCTTTATAAATGTTTTTGTAAATTATTTTGCTAA2420.12434807183413406No Hit
TATATGGGGATTGTGATTGCCAGATAAAACATCAAACCAGCATATATGTAGT2420.12434807183413406No Hit
CTTATCGGGGTCTGTGGTAATTCTCAAAACAAAATTTCACTTCTGTTTTCCA2390.12280656681139686No Hit
TCAACAGGGAAGGCAGAGCAGACTACTGATCTTAATAAAAGGAAAATTATTT2390.12280656681139686No Hit
TAGTAAGGGATCTGATTCTGGCAGAGAGGGCTCCTCCATCTGAAACGCATGG2360.12126506178865967No Hit
ATTACGGGGCAATAAAAGATATTAAGAATTGTTATCCTTAACTTACGGGCTT2360.12126506178865967No Hit
TTGTGTGGGACCTGTATGACCTTGTGAAATACATTACTTATTCATTTTATAT2360.12126506178865967No Hit
CGCTACGGGTGGAAAAATAAATTATATTTGCCTCTGGAAAGGTCTTACGTGA2360.12126506178865967No Hit
GCCATGGGGATTTCTAAAGATGAAGTAACAGGGGAAGTGATGAACTTTATAA2350.12075122678108059No Hit
GACCTCAGGTGATTCAGCCACCTCGGCCTCCCAAAGTGCTGGGATTATAGGC2340.12023739177350153No Hit
ACATAGGGGCAGGAGAAGTATACCAAGCCAAATGGTGACAAGGAACTCTCTG2340.12023739177350153No Hit
ATGTCCGGGAATGATTGCCATTCTAACTGGTGTGAGATGGTATCTCATTGTG2330.11972355676592246No Hit
TTTGTAGGGATTCTCTGCTAGTGGAGATGCAAACTGACACAGCTCTTCTGGA2330.11972355676592246No Hit
GTCAAGGGGTTTCTATGAAAGAGACGGTTACTGATATTGATAGGTAGAATGT2320.11920972175834339No Hit
ATTATCGGGCAAATGAGAAAGTGAGACACAGAGATGCTAAGGAATTGCCCAA2280.11715438172802714No Hit
CAGCTGAGATTGTACCACTGCACTCCTGCCTGAGTGAAAGAGTGAGACCGTG2280.11715438172802714No Hit
AAAGCTGGGGGAAATAGCAATATTAATTGAAGCTTACAAGGGGCTGCCCAAA2270.11664054672044807No Hit
GCTTACGGGGCGCGGCGCGGCCCCCGAGCGCACCGGGCCTGCCGAGGAGCGC2260.116126711712869No Hit
GTATCAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA2240.11509904169771086No Hit
GCTCTTGGGGAGGTAAGATTGTTCTGTCAGAAAACCCTCTCCCAGTTTCCCC2240.11509904169771086No Hit
ACTTATGGGATGAGGGCTCCGATATAAGGTGGCTGTCCTTTAATGATATATC2230.1145852066901318No Hit
ATCTGGGGGACACTGCCCTTGGCAGAAAAGTTTTCTAAAATATAATAATAAA2230.1145852066901318No Hit
CAGAGGGGTAACATTTTAATTATAGTACATCAGATTCATCTTTGTATCCCCA2220.11407137168255273No Hit
CTATTGGGGATTTTTGGGCAGGTATGTACTGGCAGTAAGTTGAAAACGTTAG2220.11407137168255273No Hit
ATTATTGGGTAGAATTGTATTCAAATAAAATATAAAAAAGAAAGATCACTTA2220.11407137168255273No Hit
GAATTGGGGGCAAATCGGCAGTATAGTATAGAGGAAAGGAGGGTGACCATTA2220.11407137168255273No Hit
TCTACCGGGAAGGGATTACACCAAATATACAACTGGAGGATTTTCCTACATC2210.11355753667497367No Hit
CACCTGGGGAGGGAAACAAAATGTGGTCATTCATCAGTCACTTAGTCATTGA2200.11304370166739461No Hit
GGGCTGGGCACTTGCCAAAATAATACATACTTCATAGACCACTGAGTTCTAG2200.11304370166739461No Hit
CCGTCGGGTCATGATAACAACTCTCAGTAAACTAACAGTAGAAGCAAACTTC2200.11304370166739461No Hit
TCAGCTGGGATTAAATGAGTGCTATCTGTGAAATTGGTTATATTAGGTGGCT2200.11304370166739461No Hit
TGATTGGGGCACATTTCATGTCTTTGAAAGTGTTGTACATTACTGTAAACCA2190.11252986665981554No Hit
CAAGTAGGGTGTCAAATCGAAGCTGATTTGAAGTTAAAGGAATAAGGTATAG2190.11252986665981554No Hit
GTCAACGGGCTTGTGGTTTAGGTTTAAAACCTAGAATAAAAGACAGAAAGCT2190.11252986665981554No Hit
CGTAACGGGGCATGGTGAAACCCCGTCTCTACTAAAAATATAGAAAAATTAG2190.11252986665981554No Hit
GCTGCAGGGGTGAGACTAAGAATGTCTGACACCTGCCTTCCTCACTTCCCAA2190.11252986665981554No Hit
GTGTTGGGGGTCAAGTGAATGTATTTTGAAAATCTTCTCTTCTGTCTGTCTT2180.11201603165223646No Hit
CTTTGCGGGTATGGGATTTTGATATTTTGGTATTCTAAAGAATTTAGTATTT2180.11201603165223646No Hit
CAATTGCGGGAGGCTATCAGAGCTGGGTCACAATTTAGTGGAGAAGATGAGT2180.11201603165223646No Hit
TCTGGGGCAATCCGTTGATCCAGAAATTATAGCTCGAGGTACTGTTGGCTTT2170.1115021966446574No Hit
GATAGTGGGGAAGGGGCAGGCGAGGCGGGGGGTGAGGAGGCTGACGAGAAGC2170.1115021966446574No Hit
CTATGAGGGCTTGCTTCTTGTGTTTGAAACACTCGTGTGCATGAGAATGTCA2170.1115021966446574No Hit
GTCTGTCGGGAACCCTCTGAAGAGAAGACTTCTGAAAGCAAAAAGACTTACA2160.11098836163707833No Hit
GCAGCTGGGCTTCCGGTCGCGGGACACCGGGTGTAGAGGGCGGTCGCGGCGG2160.11098836163707833No Hit
TGCACAGGGACCAGGCTTTGGAAATCCTTAATAATAAGGAGCAAATTTCCAG2160.11098836163707833No Hit
AACGTAGGGGTGCATCATAAAGGAATATTCTCGTTCTCTTTACTAATCTATT2160.11098836163707833No Hit
ACATCGGGTGTAAAGATACAGGTACAAGCATGTTTATTGCAGTATTGTTCTT2150.11047452662949926No Hit
CTACTTGGGTCACAAAAAACTTCCAATGCCATTTTTGAGAACTAACCTAACT2150.11047452662949926No Hit
TATAAGGGGGCCTTTCTCCTTTCAGTATTTCAGTGTGTTTATGGTTGCTGCT2150.11047452662949926No Hit
CTTCGTGGGGTATCTTCAAAGCCAACAACAGTGGGTCAAGTGCCAAATTCTT2150.11047452662949926No Hit
AATCTAGGGGGAGTCAAACACTGGATGCAGAAATTTTGGATTTTTCATCACT2140.1099606916219202No Hit
TCGTTAGGGATTTCAGATGGGCTTTTGATGCACTGTTGCCAAGGAAGGCTTT2140.1099606916219202No Hit
GATCTTGGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCC2140.1099606916219202No Hit
TCGACAGGGAACACAGTGAAGGTAGATGACACTTTCAAGAGCTTTGTGAATT2140.1099606916219202No Hit
CATGCAGGGAACAGAGTTAAAGAATTAAGAAGGGAAAATGAAGATACTGAAC2140.1099606916219202No Hit
TGCAAAGGGAAGAAAGTCATGCTGAATCTGAGAATGCCAGGACATTTCACGT2130.10944685661434114No Hit
TTCTTCGGGGGATGGCAGACATTAACTGCGTGCCTGGCCCTATGAGGGCGGC2130.10944685661434114No Hit
ACAAGGGAAACTACAAAACACTAATGAAAGAAATAAACAAATGGAAAGACAT2120.10893302160676208No Hit
TTTGATGGGGGGGATTCCAAAAGGGGCTGCAGATTCTAGACTGAAGATAATG2120.10893302160676208No Hit
ATGGTTGGGATATCTAAGTTCAAATAGATAATATTTTAATGAGAAAATACCA2120.10893302160676208No Hit
TTGGCTGGGGACATTACAAGAGTATAAAAATGGACATTAAATCATGGCCTTG2110.10841918659918301No Hit
CAAACGGGGAACATACATTGCTTGGCCAAAGCCATCAACCAGATTGCTGCAG2110.10841918659918301No Hit
ATTAATAGGGACCACCATCACTACCACCACCACCACCATCACCACCACCACC2100.10790535159160394No Hit
ATCGCCGGGGTGAAGGCAAGGGCCATGTCTATCTTATTCGTCATTAGATTCC2100.10790535159160394No Hit
TTCAGAGGGGTGGTTTCTAACTCCTGAGCTCAAGTGATTGTCCCACCTTGGT2100.10790535159160394No Hit
TAGTGTGGGAGGAAAACTTAGAACTTTGTGTGAAATAGACTGGCCAGCATTA2090.10739151658402486No Hit
CGAACAGGGACCGAAAAATTGCAGAAGCACAAGCCAAACTGGCCGAAGAACA2090.10739151658402486No Hit
CAACTGGGCTCAGCAGTGCAGTTGTGCTTCAGTCTGCAAGTCTAGCTAGCTT2090.10739151658402486No Hit
GTGGCTTGGGATCCTCTACCTATAGAAACTGATCTTATCATCTTCCTACCTC2090.10739151658402486No Hit
AGCCTTGGGGAGGAAGTCAAATTGTCCCTGTTTGCAGATGACATGATTGTAT2090.10739151658402486No Hit
CTGCTGGGGTAGAGAGCTTCAAATAGTTTCTATGTGCCATAATCACTCATTT2090.10739151658402486No Hit
AGAAGCGGGGGGAGAGGGTAATGTTACTTCACAAAGGACATGTCAGATCCTT2080.1068776815764458No Hit
TGGTTCGGGGGGGGAGCAGGAAGAGGGGCAGAAGGAAGAAGAAGAGGGGGAG2080.1068776815764458No Hit
AGCTGTGGGAGAAATTTAAGAGTTGCTTATATTTGGAACCACTGTAGTACTT2080.1068776815764458No Hit
GCCATGGGGATGAATAGAACATTACCCCATATCTCTATTAACATTGAATGTA2080.1068776815764458No Hit
AAATGGGAAATCAAGACAGTTCTTTCCAAGTCACTGAAAATTTAATGTCAGA2070.10636384656886673No Hit
CACTCAGGGCTTCTGTGTTGAAAGGATTCTCTTAAAGATCTTACAGTGGTTT2070.10636384656886673No Hit
CTCTCCGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC2060.10585001156128766No Hit
GTACACGGGGCCTCAATCCACACTGACTTATGGAAAAAATTTACTGGGTTGC2060.10585001156128766No Hit
GGTGTCTGTTTTTTGTTTGTTTGTTTGTTTTTACCATATAATTATAATCATT2060.10585001156128766No Hit
CACTAAGGGTGTCCCTTGGAACAGCTAGTGTTCATGTTTATGTCATCCTTGG2050.10533617655370861No Hit
TGAAGTGGGGTATTTGTCTTTTTATTTTATCTGTATAATTTTTTTTGTCAGA2050.10533617655370861No Hit
ACACATGGGGTAAGCATCAAGCAGCCTGTCAGGAAAAGGATGAACAAAAAAG2050.10533617655370861No Hit
GAGTGCTGGGGTGTATTAGAGATGTAGCCAAAATACAAACAGATAAGTAAAA2050.10533617655370861No Hit
TTTAAAGGGACGTGCTTCCGCTGGAGCCTCTCGGGAGGCGGGTAACGTTATA2050.10533617655370861No Hit
ATTGGAGGGATTCTCTAGGAACTCACTTTTGAGAGTATTTTTCTCTAACCCA2040.10482234154612954No Hit
GCCAAGAGGGTGACATGCCGAGTCACAAGAAAGGCCTGTTTTACCTATTCCC2040.10482234154612954No Hit
GCGTTGGGGAATACAAGCCAATATCAGCTTCCCATTGTGTAATAACTTGGGT2040.10482234154612954No Hit
TTTTGAGGGATCTTGTTTTCTAATCTATTCACTTGCCCAGGTCAGAAATCTA2040.10482234154612954No Hit
TCCTAAGGGTATTGATACAAAAAAAGCCTCCACGAATGTGTTTCTGTTACCA2030.10430850653855048No Hit
TGACTAGGGCACTTGACTCAGTGGTTTCACAGGGTCTCACCCTCACCTTCTA2020.10379467153097141No Hit
TTAACAGGGCTTGTTCCAAGGGAGCATGATTGGAAGCTTTATCGATATATAC2020.10379467153097141No Hit
TACTGCGGGTGTTCAAAAAACCACAGCACAAAAAATAAACTTGTTTTATTCT2010.10328083652339234No Hit
GAACTTGGGGCCGACCTGCAGCGGGTCATCGCCAACTGTCGCGAGTACAACC2010.10328083652339234No Hit
CACAATGGGTCCAAGGCCTAATAGGAGTGCAGCAGCCACCTCAGAGTCAGAA2010.10328083652339234No Hit
TAAAAGGGTACCATATCCACTGAAAATATCTTCCAGGAATGAAGGTGAAATA2010.10328083652339234No Hit
AATGCAGGGAGTCCACCCCTTGGAGCTAGACATCCTGTACTTAGTCACGGGG2000.10276700151581326No Hit
GCTTGTGGGATGGTACACTCAACCAATCTCAAAACGAATCCAAGGGCACCAG2000.10276700151581326No Hit
GACAGCACCTGAAATTATCCTGCCAGGAGCCTTCTGTTACTAATCATAAGCA2000.10276700151581326No Hit
GTGCTGAAGGGGATAAATGATTCTTTTTATGCAGACTAGCAAATGAAATAGG2000.10276700151581326No Hit
TCTAGAGGGTATGATTCTTGAGGTAACTCTACCAGATACTTCCAGATTTAGA1990.1022531665082342No Hit
TTGCACGGGGCTGGGATTACAGGCATGAGCCACCATGCCTGGCCAGAAGATA1990.1022531665082342No Hit
TCGACTGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1990.1022531665082342No Hit
GAACTCGGGGAAATGGGATCAGTACTCAGATGTTAAAAGTTAAGGTCTTGCT1990.1022531665082342No Hit
GGACGGGACCATCAGGAGAAGGAGTTTGAGACTGTGTATGCAACCCCCAATA1990.1022531665082342No Hit
CATGAGGGGTTTCTGGCAAAATGTGTTTACGTATCTGACAAGTTGTACATTT1990.1022531665082342No Hit
TGAAGAGGGGATCTCACGATGGGCAAATTCTGAATGGAGAGTAAAGCCCACA1980.10173933150065513No Hit
CACTTTGGGTGTTCTATGTGTATTTAAACTGGGAGGTCTGAACTAAATGATC1980.10173933150065513No Hit
AGAACGGTATGGTAATTTAATTATTTTGCCACAATATGAAGTTTCTTATGGA1980.10173933150065513No Hit
CTTTCTGGGAATCTTCATGTTCTCATTTCAACAATGTTCACAGCATCTTCAC1970.10122549649307606No Hit
ATGAAAGGGGACAGGATTTGTTTATTTTCTAAAATTAGCTTCATTCAATATT1970.10122549649307606No Hit
ATGCAGGGCAGAGGGAGGCAGTGGGTGAGAGGAGCTTCCTTTCATTTTTGAA1970.10122549649307606No Hit
AATTGCGGGATTGTGAGAGGGACTCTTTGCAATTTCTCTGATTTCTTTGTAT1960.10071166148549701No Hit
CTTTCAGGGTGATAGGAATGTAATTGGATTATTTTCTAAAAAACATTTTAGA1960.10071166148549701No Hit
TACAGCGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1960.10071166148549701No Hit
TTTTATGGGTGGTGGTGGGTAGGCAGGCATTTCCTCAGGCCTCTCAGTAGTA1960.10071166148549701No Hit
AACACCGGGGGGTCTCCGCATCCCTCTGTTCCCTGTCCCAGCAGAGGCTGTG1960.10071166148549701No Hit
TGATTAGGGATAGCCAATCTATGTCAGTTCCATAATTTTTAAACTTAGAACA1960.10071166148549701No Hit
TCATTTGGGCGATCTCGCGTGGGGTGTCCTTAAAGATGTTGTTATTAACTCG1960.10071166148549701No Hit
CGTTACGGGGTTCCACCAAAACACGTTAGAGAATTTGTACATTAGAGAATTT1950.10019782647791795No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)