FastQCFastQC Report
Sat 31 Dec 2022
SRR5846959.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5846959.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences293570
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACGCTGAGGTATCTCGTATGC5960.20301801955240656No Hit
CTTATACACATCTCCGAGCCCACGAGACGCTGAGGTATCTCGTATGCCGTCT5570.18973328337364173TruSeq Adapter, Index 2 (95% over 21bp)
GACTATGGGAAACTATTCTAGAGGGCTCAGTTTAGTATGGGATTGTGCCACC3360.11445311169397418No Hit
AATCAAGGGAAGAAAAAAGAATTAAAAAAATGAACAAAACCTTCAAGAAATA3080.10491535238614301No Hit
CGAACAGGGTGCATTAAAACTGTTCTCTGAAATTAAGCTTGTCTTGTGAGGA3020.10287154682017917No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)