FastQCFastQC Report
Sat 31 Dec 2022
SRR5846956.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5846956.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences181824
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACGCTGAGGTATCTCGTATGC7290.40093717001055973No Hit
CTTATACACATCTCCGAGCCCACGAGACGCTGAGGTATCTCGTATGCCGTCT6920.38058782118972195TruSeq Adapter, Index 2 (95% over 21bp)
CTTCGAACGGGGTTGTCTGTTTGATGGTTTAATCTTTTGGGTTGTTGTCACT2180.11989616332277367No Hit
AGACATGGGCTCTAAAGCAAATCTGCAGTGTTCCAAAGACTTTGGTATGGAT2100.11549630411826822No Hit
AAATTTGGGATGTTCAGGTTTTGAATGTTTTTGTATCAGTTTTTTCCTTTGT2000.1099964801126364No Hit
TCATCGGGGATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTA2000.1099964801126364No Hit
ACTTTGGGACACCAGGAGAATGGCTTCTCCACCAACGGACCCTCTGGGGACA1990.10944649771207321No Hit
GCATGTGGGCACTGTGCAGGGAATAAAAGCTGACAGGCTTTTCTGATAGACG1970.10834653291094684No Hit
TTCGTCGGGGTAAATGTGTGTTCCTCCTAGGTTGGAAGAAATGTCTTTCCTT1970.10834653291094684No Hit
CACTGGGGGGCGCGGGGATGGGAGGAAGAAATTTTCTCAGAGCAAACTTTCT1960.10779655051038367No Hit
TCACATGGGGATTGCAGTAGATTTTGGAAATAATGACGAAAAACAAATTGAG1950.10724656810982049No Hit
AAAAATGGGATCTCATGATTCCTACTTGTACAGAATGATTAGTTTTGCTTCT1920.10559662090813093No Hit
TGAACTGGGAAGCGCCCATCCTCGTGGTAGGCAACAAGCGGGACAGGCAGCG1910.10504663850756776No Hit
TAAGTGGGGGGACCAGTTAGTCCCTGAGAATCAAAACATCACTGTTTACTCT1900.10449665610700457No Hit
GCAAAGGGGATGCCATTGCACTCCAGCTCGGTGACAATAGCCAGACCCCGTC1900.10449665610700457No Hit
TTACTAGGGTGGCATTCCACGAGATTTTATAACCAGAGTCTAGCAGTGATAA1890.1039466737064414No Hit
TTTAAAAAGAGATTAAACCGAAGGTGATTAAAAGACCTTGAAATCCATGACG1880.10339669130587821No Hit
GCTCCGGGTCCGATCTATTAGTGGGGTTTTACTATAAGTAAGTTGTACCTCA1870.10284670890531503No Hit
ACGCTAGGGACAGCAAGACCCTGTCTCAAAACAAACAAACAAACAGAAATGT1870.10284670890531503No Hit
TATTAAGGGAGTCGTTTTTGTTCCAAGAATGATATGAAAGGTGAAGAAGAGG1860.10229672650475186No Hit
TTTACTGGGTCTTCGTTAGGGGTAACCAGCATTGTAGCTTTGACAATGAACA1850.10174674410418866No Hit
AAGTTGGGGCATAAATCCCTGTTTAGTGTTACATGGGAATAAGGAATTTCAT1840.10119676170362549No Hit
ATTTCTGGGACAAGGAACTTCAGGCACATGGTGCTGATGAGTTATTAAAGAG1830.1006467793030623No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)