FastQCFastQC Report
Sat 31 Dec 2022
SRR5846955.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5846955.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences182483
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACGCTGAGGTATCTCGTATGC7400.4055172262621724No Hit
CTTATACACATCTCCGAGCCCACGAGACGCTGAGGTATCTCGTATGCCGTCT7290.39948926749341035TruSeq Adapter, Index 2 (95% over 21bp)
GCTGCCAGATCCTGGCCAGCCTGCAGTCATGCGAGAGCAGTAAAAGCAAGTC2220.12165516787865172No Hit
TCATCGGGGATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTA2130.11672320161330096No Hit
GCATGTGGGCACTGTGCAGGGAATAAAAGCTGACAGGCTTTTCTGATAGACG2100.11507921285818405No Hit
GGTAACGGGATGATTAAAAACGCATTTAATACTAACTGCAGTAGTTCTTTCA2060.11288722785136149No Hit
TGAACTGGGAAGCGCCCATCCTCGTGGTAGGCAACAAGCGGGACAGGCAGCG2020.11069524284453894No Hit
AAATTTGGGATGTTCAGGTTTTGAATGTTTTTGTATCAGTTTTTTCCTTTGT2020.11069524284453894No Hit
TTCGTCGGGGTAAATGTGTGTTCCTCCTAGGTTGGAAGAAATGTCTTTCCTT2020.11069524284453894No Hit
TTTGATGTGGACAGATTATAGAGGTAATTGTGAAGACAGTTGAACCTTAGAA1930.10576327657918819No Hit
AAGCAAGGGGGAACACTGTGATTAATTAGCCAACGCCAGAGATCTACATGAG1930.10576327657918819No Hit
AGACATGGGCTCTAAAGCAAATCTGCAGTGTTCCAAAGACTTTGGTATGGAT1910.10466728407577691No Hit
TCACATGGGGATTGCAGTAGATTTTGGAAATAATGACGAAAAACAAATTGAG1910.10466728407577691No Hit
TTTACTGGGTCTTCGTTAGGGGTAACCAGCATTGTAGCTTTGACAATGAACA1900.10411928782407129No Hit
CTTCGAACGGGGTTGTCTGTTTGATGGTTTAATCTTTTGGGTTGTTGTCACT1880.10302329532066001No Hit
AATCTGGGTATCAGCCACCCAGTAAAAATCAAAAAATCTGGTATGCTGTTTG1870.10247529906895438No Hit
ACTTTGGGACACCAGGAGAATGGCTTCTCCACCAACGGACCCTCTGGGGACA1870.10247529906895438No Hit
CCTACAGGGCACTTCCTAGAATTTTAGAAACCATCACTGTGAAAGAATTCCA1860.10192730281724874No Hit
GAGGGTGGGTCCTGTAATATATGGGTATTGATGAGTTTTCAGGCATATTTTT1860.10192730281724874No Hit
CTGTGGGAGAATTATTTAATTTTACTTGTAATTTTAACATTAAAAACTTCAG1860.10192730281724874No Hit
ATGCAGGTAGGGGATGCTGTTCCCGCTGCACGGCCCACCGGCGGCGTAAGTG1840.10083131031383746No Hit
GGTACAGGGGGTATTCAGAGAAGTATTTTGCATTATAGTAATAGGGAAACAT1830.1002833140621318No Hit
ACAACTGGGGAAAGAAGTTTGCTGACGAAGATGGCGACTGAGGCACAGAGTG1830.1002833140621318No Hit
CGAAGCGGGGATATTCAGCAGCTACAAAAACAGCTAAAGGAAGCAGAACAAA1830.1002833140621318No Hit
AAAAATGGGATCTCATGATTCCTACTTGTACAGAATGATTAGTTTTGCTTCT1830.1002833140621318No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)