FastQCFastQC Report
Sat 31 Dec 2022
SRR5846954.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5846954.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences178116
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGCTGAGGTATCTCGTATGCCGTCT7310.4104067012508702TruSeq Adapter, Index 2 (95% over 21bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACGCTGAGGTATCTCGTATGC7020.39412517685104087No Hit
ACTTTGGGACACCAGGAGAATGGCTTCTCCACCAACGGACCCTCTGGGGACA2060.11565496642637382No Hit
TTTAAAAAGAGATTAAACCGAAGGTGATTAAAAGACCTTGAAATCCATGACG2050.11509353455051764No Hit
CACTGGGGGGCGCGGGGATGGGAGGAAGAAATTTTCTCAGAGCAAACTTTCT1970.11060207954366817No Hit
AGACATGGGCTCTAAAGCAAATCTGCAGTGTTCCAAAGACTTTGGTATGGAT1970.11060207954366817No Hit
AAATTTGGGATGTTCAGGTTTTGAATGTTTTTGTATCAGTTTTTTCCTTTGT1950.10947921579195581No Hit
AAAAATGGGATCTCATGATTCCTACTTGTACAGAATGATTAGTTTTGCTTCT1950.10947921579195581No Hit
CTTCGAACGGGGTTGTCTGTTTGATGGTTTAATCTTTTGGGTTGTTGTCACT1900.1066720564126749No Hit
CTTGCAGGGAGAAGTAAAAGCCCCCAAATAATTCATCTTTTAGGCTTTGTAA1900.1066720564126749No Hit
CAGCATGGGTGTCAAGAGTTGACAGCATATTAATTGTGTGGCATTATAATTG1900.1066720564126749No Hit
CTGTGGGAGAATTATTTAATTTTACTTGTAATTTTAACATTAAAAACTTCAG1890.1061106245368187No Hit
ATGGCTGGGATTAATAGATGTAAATCTTCATTGATTTATATGTGTTCTCTAA1870.10498776078510634No Hit
ACTCGCGGGAGACCAGTCTAGAGAATGTGAAACCTCTATAAACCAAAACATA1850.10386489703339397No Hit
TGTACGGGAACTGCTCCAAGGAGTTGTGCAAACTTTTCATTCCATAACAGTC1850.10386489703339397No Hit
GCTCCGGGTCCGATCTATTAGTGGGGTTTTACTATAAGTAAGTTGTACCTCA1840.10330346515753779No Hit
TCATCGGGGATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTA1840.10330346515753779No Hit
GCAAAGGGGATGCCATTGCACTCCAGCTCGGTGACAATAGCCAGACCCCGTC1820.10218060140582542No Hit
AATCTGGGTATCAGCCACCCAGTAAAAATCAAAAAATCTGGTATGCTGTTTG1820.10218060140582542No Hit
GCTGCCAGATCCTGGCCAGCCTGCAGTCATGCGAGAGCAGTAAAAGCAAGTC1810.10161916952996924No Hit
CTATTTGGGATATGGAAGATATGTAAGATAAATCTGATCAAATGAATTGCAG1800.10105773765411305No Hit
GCACAGGGGTCTGCGCACGGGATTCTGGCTGAGAACGGCTTCCTCCCAATTC1800.10105773765411305No Hit
TTACTAGGGTGGCATTCCACGAGATTTTATAACCAGAGTCTAGCAGTGATAA1800.10105773765411305No Hit
CTAACTGGGCATGCTTGTCTCTGTGTCTCTGTCTTTGTTCACACTACTTATC1790.10049630577825687No Hit
TTTGATGTGGACAGATTATAGAGGTAATTGTGAAGACAGTTGAACCTTAGAA1790.10049630577825687No Hit
TGCAGCGGGGCGTACTTGCTTTGTAAGTGATCTGAAGCGAGGACTAAAAATC1790.10049630577825687No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)