FastQCFastQC Report
Sat 31 Dec 2022
SRR5846949.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5846949.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences168842
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACGCTGAGGTATCTCGTATGC7250.42939552954833515No Hit
CTTATACACATCTCCGAGCCCACGAGACGCTGAGGTATCTCGTATGCCGTCT6260.37076082965139007TruSeq Adapter, Index 2 (95% over 21bp)
TTTAAAAAGAGATTAAACCGAAGGTGATTAAAAGACCTTGAAATCCATGACG2840.16820459364376164No Hit
TCGTTTGGGGGTCCCAATGGACCTTATTTCTGTTCTACAATACATTAAGTTT1990.11786166948981888No Hit
CAACTAGGGGAGTCTTACCTCGTTGTGGCGTCAGAACCGCCGTCGCTCGCTC1980.11726939979389014No Hit
GAAGTTGGGTCTGCATCGGGGGTCCGGGTCGCCGCCGGCAGCAGCCTCCCGG1980.11726939979389014No Hit
GTTTAAGGGATCAACCAACAAGTCATTATTACCCTCACTGTCAACCCAACAC1920.11371578161831772No Hit
AGGCTCGGGATTTACCTTTGCTTGGAAACCTCTCCAGCTAAATATCCAATTT1910.11312351192238898No Hit
GCGTTAGGGGCGATGGAGGAAAATTTACCAAGCAAATGGAAAGCAGAAAAAA1900.11253124222646024No Hit
AGACCTGGGCTGTAATGGTCTCTAACATGAATAACAAGATTAGAGGGACAAT1880.11134670283460277No Hit
GCTTTAGGGCAGAAAACAAAAGTGGAGTTAATAACCGTTTTTAAAATACTAC1860.11016216344274529No Hit
CGTGCGGGGGAGGTCTGTTAGATGAAAGGATATGAGAAAGGTAATATTGGTA1850.10956989374681655No Hit
TTTCTTGGGGCTCTGGGTCTGGCTGAATTTTCTGCCCTGAGATCTGGGCATA1840.10897762405088782No Hit
CTTAGGGGGGGTGTGGGTCGAGCAGCGGCGGCGGCGGCGGCAGTGGCGGTCC1810.1072008149631016No Hit
CGCTATGGGGTTTTTACAGATCAGACTTGACTGTACTAATTAGACAGTCAAT1810.1072008149631016No Hit
GCGCTTGGGAGGGAGCATGTAGCTAAAATGAAGAAGATGGAGATGGAGATGG1810.1072008149631016No Hit
CGCAACGGGGGCTCTTTTTCCGGCTGGAACCATGGAGGGTGTAGAAGAGAAG1800.10660854526717287No Hit
AAATACGGGTTGTTGCGAAGGTTTCAAATATTTGTGACTGAATGTACGGTGA1780.10542400587531539No Hit
GGAAAAGGGAAGAGTATTTTTCTTAAAAGTCTAGATTTTCTTTTGTTTAGTC1780.10542400587531539No Hit
ATACAGGGGACCCCAGTCTTCAGCCTGAAGAGTGCCTTCCATTGGGCTTCCA1770.10483173617938664No Hit
ATTGTTGGGAAATATCTGAACTGACAAAATTTTAATTTAAGTTTTGTACCTT1770.10483173617938664No Hit
TTTAGAGGGGCAGGCCTTGTGATAACTAAGCGCTACTTTTGACGAGCCTTCA1760.1042394664834579No Hit
CGTTGCGGGGATGTAAGGATTGTTCAGCAGTTCGGCATTTGGGATCGAAAGC1760.1042394664834579No Hit
CTGCCGGGAGCAGGGCTGCATTTGAATAGAGGAGGAATCTTTTCCTCCATTC1730.10246265739567169No Hit
GCAGCAGATTTCTTTGATAATTTAGCTTTTACTGAATCTCTTGCAATGAAGA1710.10127811800381421No Hit
GACTGCGGGCAAAGAATAAATTGCCAATGAATTATTAAGACCAAATCATGGA1700.10068584830788548No Hit
TATGCCGGGTTGAAATCAAAATCTCAAAGAGATACCTGCACTCCCGTGTTCA1700.10068584830788548No Hit
TGGTTTGGGTGATTGAATTGGTAAGAAAGAGTTGGATTCTAGATATATTTTG1700.10068584830788548No Hit
TTATCTGGGAATTCATTGAAGTAATAACCAAATAATTTTCAATCTTGATTCA1700.10068584830788548No Hit
TCAACTGGGACTGGAGAGTTCATCAATCACAGTTTCAGAACATGCTCTTAAT1690.10009357861195674No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)