FastQCFastQC Report
Sat 31 Dec 2022
SRR5846948.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5846948.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences163052
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACGCTGAGGTATCTCGTATGC6880.4219512793464662No Hit
CTTATACACATCTCCGAGCCCACGAGACGCTGAGGTATCTCGTATGCCGTCT6880.4219512793464662TruSeq Adapter, Index 2 (95% over 21bp)
TTTAAAAAGAGATTAAACCGAAGGTGATTAAAAGACCTTGAAATCCATGACG3250.1993229153889557No Hit
ATCAGTGGGGGAAAATGCCAGAGGGGTGGTCAGAGGAGGATGCTGGAAACGG2120.13001987096141107No Hit
GGAAAAGGGAAGAGTATTTTTCTTAAAAGTCTAGATTTTCTTTTGTTTAGTC1990.12204695434585285No Hit
GCTTTAGGGCAGAAAACAAAAGTGGAGTTAATAACCGTTTTTAAAATACTAC1930.11836714667713367No Hit
GCGTTAGGGGCGATGGAGGAAAATTTACCAAGCAAATGGAAAGCAGAAAAAA1900.11652724284277409No Hit
AGTAATGGGGATCTTTCAGCGGGAATTGGAACCTTCTGTATACTGCTGGTGG1890.11591394156465422No Hit
TTCACTGGGACCCAAGGCCAGGAAAAAAGAAAATTTTTGGATATCAGAAATC1870.11468733900841449No Hit
GTTTAAGGGATCAACCAACAAGTCATTATTACCCTCACTGTCAACCCAACAC1850.11346073645217476No Hit
GAAGTTGGGTCTGCATCGGGGGTCCGGGTCGCCGCCGGCAGCAGCCTCCCGG1850.11346073645217476No Hit
CTTAGGGGGGGTGTGGGTCGAGCAGCGGCGGCGGCGGCGGCAGTGGCGGTCC1830.11223413389593503No Hit
TCGTTTGGGGGTCCCAATGGACCTTATTTCTGTTCTACAATACATTAAGTTT1780.10916762750533572No Hit
TATGCAGGGTGATGCTAAGGCACTGATAGCGGACAGTGGACTTAAAATACTT1780.10916762750533572No Hit
CTCAAGGGGATTTGACATTTCTCCTATGTTCCAGTTTGTGCAACTGATAAAA1770.10855432622721586No Hit
GGTCTGGGGGGAGTCGGCTAGCCGGGGCTCGGGGAGCGGGGTGCGCAGGGCT1750.10732772367097612No Hit
TTTCGTGGGCATTAGAATTGAAAGAAAAAAAGTATATAAAGTGCTTAATACT1750.10732772367097612No Hit
AGGCTCGGGATTTACCTTTGCTTGGAAACCTCTCCAGCTAAATATCCAATTT1740.10671442239285626No Hit
TTTAGAGGGGCAGGCCTTGTGATAACTAAGCGCTACTTTTGACGAGCCTTCA1730.10610112111473641No Hit
TGCTCAGGGAGTCCGAGTGGAGAGAGCGAGCTGAGTGGTTGTGTGGTCGCGT1720.10548781983661655No Hit
CGCAGGGCCTTTCCAACTTGGACGCTGCAGAATGGCTCCCGCAAAGAAGGGT1710.10487451855849667No Hit
TGTTAAGGGTGTTTTTGATGTAGGTGTTTATTACTAAATACTTCCCTCTTAA1700.10426121728037681No Hit
TATGCCGGGTTGAAATCAAAATCTCAAAGAGATACCTGCACTCCCGTGTTCA1700.10426121728037681No Hit
ATTATTGGGGAAAATTTAAAAAGTAAAATTCACTGAAAAATAACTACTAGTC1700.10426121728037681No Hit
TTATCTGGGAATTCATTGAAGTAATAACCAAATAATTTTCAATCTTGATTCA1700.10426121728037681No Hit
AGACCTGGGCTGTAATGGTCTCTAACATGAATAACAAGATTAGAGGGACAAT1690.10364791600225695No Hit
AACGAGGGGTCATTTTACAACTTCCTTTATCCCTTTTTTCCAATTTTGTCAG1690.10364791600225695No Hit
CGCTATGGGGTTTTTACAGATCAGACTTGACTGTACTAATTAGACAGTCAAT1670.10242131344601721No Hit
GAGAACGGGTAACCAGTGCTTGGAAAAATCTTGCTTGGCTATTTCTGCATCA1670.10242131344601721No Hit
CGCAATGGGAATTACAATGACTGGTGCTATAATCTCAAAAACACCGAAACAG1660.10180801216789735No Hit
ACCTCAGGGGAATGAGTGAATATTTAGGCTTAGAATGAAAAAAATTAAATTG1650.1011947108897775No Hit
AATAGCGGGGTCTCGAGTAGGCAGTAGAGACTTGGAACCCAGCTCTCCAGAG1650.1011947108897775No Hit
ACATCAGGGAATGTTTCAAATAATGCTTTAGTTTGGACTGCCTCAGATTATG1640.10058140961165764No Hit
ACTTGAGGGCAGGTTCAGAAAAAAACTAAAGATAGGCTATATTTTATTTAGA1640.10058140961165764No Hit
GTGCACGGGATCTGGACTCTGACACTTCTTTTACTCTCCCTCTTTAATACTA1640.10058140961165764No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)