FastQCFastQC Report
Sat 31 Dec 2022
SRR5846938.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5846938.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences192412
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGCTGAGGTATCTCGTATGCCGTCT5220.271292850757749TruSeq Adapter, Index 2 (95% over 21bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACGCTGAGGTATCTCGTATGC5100.2650562334989502No Hit
ATTCATGGGGTAACTGACAAGATGAATTACTCAGTTTCTCTTTCTCTAAAGC2600.1351267072739746No Hit
AAGCCCGGGAATGTTCTTCAAAAAAGTAATATCCTCACTTGGAGAGTGTCAA2590.13460698916907468No Hit
CGTATGGGGAAGTAAGAAAAGTTTAGATTAGAGCAAGTAAGCGAAAAATCAC2560.133047834854375No Hit
CGATATGGGAAGGCACATTGATCCATTTACTCATTCATTCATTCATTCATTC2520.13096896243477538No Hit
CGGCTAGGGTATTTGCAGACTATTCATCCTGTGAGGGGCTAGGATCCAGAAT2490.12940980812007566No Hit
TGTCCAGGGTATTTTCAGCAGTAGAAATTCTTCGATGGCCCATTTCAGACCA2460.12785065380537594No Hit
GCATCAGGGGGCATTTACTGGCTTATGATGTCTGAGGCATTGATCTAAGGAC2400.12473234517597655No Hit
CTGACAGGGAAGACAGAGCTTTAGATTGCAAATCAGTTTAGCCTCATCAGTT2380.12369290896617675No Hit
GGCATAGGGACTGAGCAAATGTTTGAAATTATGTTAATTTTGACAGGTACAA2350.12213375465147704No Hit
GCCAGAGGGGCTTTGTTAGGCTGTGTGATGTTCATACATTTTCTCCCCATCT2320.12057460033677732No Hit
TCAAGTGGGAAGTATAACATTTTTCCTTTGTAGATTGGAGAAAGAAGTCATG2320.12057460033677732No Hit
TATTAGGGGCCTTCGATGCTGCTGCCCACCAGTGATGTAAATCACAAAGCAT2300.11953516412697753No Hit
AACAATGGGGGCGTAGGAGCGTGCGTTCGGGCCCTCTTCTCCCACCTGTTCG2290.11901544602207763No Hit
AAACGAGGGGTGGATTTTTGAAAATAAATATATTGGAAATATTTTTTGAGAT2250.11693657360247801No Hit
ATGACAGGGAGCACTGAAACCTGAAGTGTCTGTGATATGTATAATAAGACAG2230.1158971373926782No Hit
TATAAAGGGCTCCCAGGTTCAAGTGATTCTCTTGCCTCAGCTTCCCGAGTAG2230.1158971373926782No Hit
TGTGCTGGGATTACAGGCGTGAGCCACCGTGCCCGGCCAACAAAGAAAGTTT2220.11537741928777832No Hit
TGAACAGGGTCTGGAAAGAAACAGGATAATTTCTGTTATGATGGTGCTTTCT2210.11485770118287841No Hit
ATCACAGGGATTGGGATTTGAAGACAAAATTGTAGGAAATCTTACATTTTTG2200.11433798307797849No Hit
GTAACAGGGTATTAAACAGATGCAGTCATCATTTACATCTTCAGAACAAGAG2190.1138182649730786No Hit
TTCTAGGGGACCAGACACAGAGAAAAGGGAAATTGTGTTTTATTGAGCGATT2180.1132985468681787No Hit
ATTTCCGGGGGAGAGAAGGAAAGGAGAGAAGGCAGGAAGAAGGCAAGGGACG2180.1132985468681787No Hit
CATTATGGGCACCAATTGGCCAGCAAAACCTTTTGTTCACATAACATATTAA2160.11225911065837889No Hit
TTCACTGGGGGAGATGCCTAAGCTTTTATGAGACTTTACATAAAATCCTAAA2160.11225911065837889No Hit
CTCTGTGGGCTTTTTGTAAATGCGTCCTGATAATGATTAGGAAAATCGACCT2160.11225911065837889No Hit
GCTGTGGGGGCCGGTTGACTTTGCGGAGCCATGGAGGGCGGCTTCGGCTCCG2150.111739392553479No Hit
AGTACTGGGCGTTACTAAGTAGAATAGGTGGACCTAACATTATTTGTAGATG2150.111739392553479No Hit
ATCGACGGGAGTTCACACGTGGCGCCAGCGGAGGCAGGTTGATGTGTTTGTG2150.111739392553479No Hit
GGCAACGGGATTGGGTAGCATTAACTGTAAGTTTTGTGCTTCCAAATCACTT2140.1112196744485791No Hit
AAAAACGGGATTCCTGATCGAGGCTTGCAAAGTGAATCCTTTTGCCAAGGAC2130.11069995634367918No Hit
GACTACGGGGAGGTGAAGGGATGCCGCAGTATCGTAATCCCTTTGAAAAAGG2130.11069995634367918No Hit
GTGGTCCGGGGTGACTTTGTGAAGCAATATTTTGACCAGGTACTGTAAAGCT2130.11069995634367918No Hit
TTTTCTGGGATGACAGAAAGAAAGAGGGAGTGGTGGAAAGAAACAATAGGAT2120.11018023823877929No Hit
CAACAAGGGTATGAGCACCTGGAATACAATCAGTGGAGATATTTATTTGAAT2110.10966052013387938No Hit
ACGTATGGGAACATAATGTCCTTGGGAAATGATGATAGCCCAATGAACTTAA2100.1091408020289795No Hit
TTTGACGGGATCATTTTTATTTGCTAGCCTGTTAATATTATTCATTATATAA2090.10862108392407958No Hit
TACTATGGGTGAGCGGTTGAATTGGGAGAATGAAGATAAGTAATTTACCTGT2090.10862108392407958No Hit
ACAGTTGGGGATCTAACACCGGGATGAGAACAAAGGACAATGTACTAGAATC2090.10862108392407958No Hit
AGCAAAGGGCACAGATCCAATTTCTGAACAAAACATATCAAAAATTGAAGGT2080.10810136581917967No Hit
GCTAATGGGTACTAAGATGCTTTGCTTGCTGTGGCACTCTAAACTCTCTTGC2080.10810136581917967No Hit
TAGAAGGGGGATGCTGATTGTTTGGGTCACTTCATTATGTTGACAAATTCCT2060.10706192960937987No Hit
TTGCTGGGATTGCAGGAATGAGCCACCATGCCCGGCCAATATTTTAACATTT2060.10706192960937987No Hit
TCTTCTGGGGTGGCTTGAACTTGCCAGATGCAAATACCACAGACTCCAAGAA2060.10706192960937987No Hit
CAGTGCGGGGCTCTTTTTCCGGCTGGAACCATGGAGGGTGTAGAAGAGAAGA2050.10654221150447997No Hit
GCGTGGGGATCATTCTGAGGGGCCACAGTAATTACAAACTTACTATCCGCCA2050.10654221150447997No Hit
GGCAGGGGACTAATGATATTGAGTATCTTCTCATGTGCTTACTTGCCATCCG2050.10654221150447997No Hit
CTGCGAGGGAAATCAAAACCACTATGAGATATCATCTCACACCAGTTAGAAT2030.10550277529468018No Hit
AATTGTGGGCTTCTACATGTGGGTATCCTTTTTTCCCAGCACAATTTATTGA2030.10550277529468018No Hit
AACAGCGGGAATAAATAAAAGTAAAAATGTGTTATCTAATTTCTGTGAAGCA2030.10550277529468018No Hit
GTAAAAGGGAGAACACAGCCAGCTAGTAAGGTTTTCCCACTGGGGCTGACAT2000.10394362097998046No Hit
CCACCAGGATGGTACATTTATTAAAATGAATTGACCTGCATTGACACATCTT2000.10394362097998046No Hit
GCCACTGGGGGGGCATTTTTATATAATCAACAACTAAATCTTTTGCCAAATG2000.10394362097998046No Hit
CATCAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA2000.10394362097998046No Hit
GTATTAGGGCTCTTTCCAGCCAGCGCCGAGCGATGGGCATCTCTCGGGACAA2000.10394362097998046No Hit
CACCAGGGGGTATATTAAAATTTACTGCACACTTTGAGATTGCTGCCATTTT2000.10394362097998046No Hit
AGCTTAGGGTAGATCTGGATTTTGAACCACTTAATGAAGCGGCAACACCAGG1990.10342390287508055No Hit
AGCCTGGGTGACAGAGCGAGACTCTGTCTCAAAACACAAAAGAGAAAAGCTA1990.10342390287508055No Hit
TGAAATGGGCACTTCTGTTATTTCCCTTTTAACCCTTGCCCGCCTATGTTTT1990.10342390287508055No Hit
GGTACGGGGAGGAAGCTGAAGAAAAAGCGCCAGTGAAGAAATCTATACGAGA1980.10290418477018067No Hit
CGAAGAGGGTATAGATCTGTTGCTCACACCACACACATGTTCACACAAACGC1970.10238446666528075No Hit
AAGGGCGGGAGGGGATGAGAGTCAGGTGTAATCTTACGAGGTGTGTGTTTTT1970.10238446666528075No Hit
AATGCGGGGTCTTAAGTCTGTGGCACACTGTGTCTTCAGACAGTTTGAAGGA1970.10238446666528075No Hit
AGCCCAGACCACTCTGAACTCCAGATTCACATACCCAGCTGCCTACTGGGCT1960.10186474856038086No Hit
TGAGATGGGGGTCACAGTAAGCACCCAGTGAATGTTTGTTGACTATCGAATG1960.10186474856038086No Hit
GGTGCGGGACGAGAACGACTGCATTTTCGACAAGTTTGAATGTGCCTGGAAC1950.10134503045548095No Hit
ATGTCGGGGCTTTGTATTCCAAAATAGGGTCTCAAACATTTTGTAATTTTCA1950.10134503045548095No Hit
GTAACAGGGGTAGCAGGTTAAATAACAGTATCCTCTAAGGAGCTGAGTTCAG1950.10134503045548095No Hit
CTGTAAGGGTTCCCGGTGCCTGCTGTTGGAGTCAGCATAGCATATGATTATG1950.10134503045548095No Hit
ATAACAGGGACTGCTACTACAACCAAGCGTTATAAAGTACTCACTAAAACAG1940.10082531235058104No Hit
GCACTCGGGATCCAGTGCTAGTGGCCAGACTCTTCATTCCAGAGTTATTCAC1940.10082531235058104No Hit
GACACCGGGGTATCCCAGAATGTGTTGTGTTTTTTTAAAAGATGCATAATAG1940.10082531235058104No Hit
TGTAGCGGGTAGCAATTGTTCAGTTGGCAAAGAATGTGAAAGTCTGGGAAAA1930.10030559424568114No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)