FastQCFastQC Report
Sat 31 Dec 2022
SRR5846937.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5846937.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences194185
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACGCTGAGGTATCTCGTATGC5460.28117516800988746No Hit
CTTATACACATCTCCGAGCCCACGAGACGCTGAGGTATCTCGTATGCCGTCT5310.2734505754821433TruSeq Adapter, Index 2 (95% over 21bp)
CGTATGGGGAAGTAAGAAAAGTTTAGATTAGAGCAAGTAAGCGAAAAATCAC2600.13389293714756548No Hit
ATTCATGGGGTAACTGACAAGATGAATTACTCAGTTTCTCTTTCTCTAAAGC2570.13234801864201665No Hit
CGGCTAGGGTATTTGCAGACTATTCATCCTGTGAGGGGCTAGGATCCAGAAT2560.1318330458068337No Hit
GCTGTGGGGGCCGGTTGACTTTGCGGAGCCATGGAGGGCGGCTTCGGCTCCG2550.13131807297165074No Hit
TTTTCTGGGATGACAGAAAGAAAGAGGGAGTGGTGGAAAGAAACAATAGGAT2530.13028812730128486No Hit
ATTTCCGGGGGAGAGAAGGAAAGGAGAGAAGGCAGGAAGAAGGCAAGGGACG2500.12874320879573603No Hit
CGATATGGGAAGGCACATTGATCCATTTACTCATTCATTCATTCATTCATTC2450.12616834461982132No Hit
GGCATAGGGACTGAGCAAATGTTTGAAATTATGTTAATTTTGACAGGTACAA2440.12565337178463837No Hit
TGACCCGGGTTCTCCATCCTGCAAGCCACTCCTTTTACCTCCACTGTAACTC2420.12462342611427248No Hit
TGAGATGGGGGTCACAGTAAGCACCCAGTGAATGTTTGTTGACTATCGAATG2400.12359348044390658No Hit
AATTGTGGGCTTCTACATGTGGGTATCCTTTTTTCCCAGCACAATTTATTGA2390.12307850760872364No Hit
ACAGTTGGGGATCTAACACCGGGATGAGAACAAAGGACAATGTACTAGAATC2380.12256353477354069No Hit
TCAAGTGGGAAGTATAACATTTTTCCTTTGTAGATTGGAGAAAGAAGTCATG2370.12204856193835775No Hit
AAAAACGGGATTCCTGATCGAGGCTTGCAAAGTGAATCCTTTTGCCAAGGAC2360.1215335891031748No Hit
TGAACAGGGTCTGGAAAGAAACAGGATAATTTCTGTTATGATGGTGCTTTCT2360.1215335891031748No Hit
ATCGACGGGAGTTCACACGTGGCGCCAGCGGAGGCAGGTTGATGTGTTTGTG2350.12101861626799186No Hit
AGATTGGGGCCTGACAAAACACATAGCCTACCCCTTCCTTGTACTATCCCTA2340.12050364343280892No Hit
TTCTAGGGGACCAGACACAGAGAAAAGGGAAATTGTGTTTTATTGAGCGATT2310.11895872492726008No Hit
GCGTGGGGATCATTCTGAGGGGCCACAGTAATTACAAACTTACTATCCGCCA2310.11895872492726008No Hit
AACAATGGGGGCGTAGGAGCGTGCGTTCGGGCCCTCTTCTCCCACCTGTTCG2300.11844375209207714No Hit
GCTAATGGGTACTAAGATGCTTTGCTTGCTGTGGCACTCTAAACTCTCTTGC2290.11792877925689418No Hit
GGCAACGGGATTGGGTAGCATTAACTGTAAGTTTTGTGCTTCCAAATCACTT2290.11792877925689418No Hit
TATAAAGGGCTCCCAGGTTCAAGTGATTCTCTTGCCTCAGCTTCCCGAGTAG2280.11741380642171124No Hit
CTGCGAGGGAAATCAAAACCACTATGAGATATCATCTCACACCAGTTAGAAT2270.11689883358652832No Hit
CAGTGCGGGGCTCTTTTTCCGGCTGGAACCATGGAGGGTGTAGAAGAGAAGA2260.11638386075134538No Hit
AGCAAAGGGCACAGATCCAATTTCTGAACAAAACATATCAAAAATTGAAGGT2250.11586888791616243No Hit
CGTTCAGGGATGGAAGTGGTAGAGATATAATTAAAGGTCATTTTGCTACTTG2240.11535391508097949No Hit
CTGACAGGGAAGACAGAGCTTTAGATTGCAAATCAGTTTAGCCTCATCAGTT2240.11535391508097949No Hit
GACACCGGGGTATCCCAGAATGTGTTGTGTTTTTTTAAAAGATGCATAATAG2240.11535391508097949No Hit
AGTACTGGGCGTTACTAAGTAGAATAGGTGGACCTAACATTATTTGTAGATG2190.11277905090506476No Hit
GTATTAGGGCTCTTTCCAGCCAGCGCCGAGCGATGGGCATCTCTCGGGACAA2190.11277905090506476No Hit
CAACAAGGGTATGAGCACCTGGAATACAATCAGTGGAGATATTTATTTGAAT2170.11174910523469887No Hit
ACGTATGGGAACATAATGTCCTTGGGAAATGATGATAGCCCAATGAACTTAA2170.11174910523469887No Hit
GTATCAGGGATATTGTGAAAATGACCATAATGCCAAAAGCAATCTACAAATT2170.11174910523469887No Hit
GTGGTCCGGGGTGACTTTGTGAAGCAATATTTTGACCAGGTACTGTAAAGCT2160.11123413239951592No Hit
GCATCAGGGGGCATTTACTGGCTTATGATGTCTGAGGCATTGATCTAAGGAC2150.11071915956433298No Hit
CATAAGGGGTCTTGTATAAATAGGAGTCATGGGCGTTAGTTCTGTAGTAATA2150.11071915956433298No Hit
ATAACAGGGACTGCTACTACAACCAAGCGTTATAAAGTACTCACTAAAACAG2150.11071915956433298No Hit
CTCTGTGGGCTTTTTGTAAATGCGTCCTGATAATGATTAGGAAAATCGACCT2150.11071915956433298No Hit
AACAGCGGGAATAAATAAAAGTAAAAATGTGTTATCTAATTTCTGTGAAGCA2150.11071915956433298No Hit
TGCTTCCGGGATGATTATAAATTTTAAAATGAGTTTTTAGCTATAGTAATCT2140.11020418672915003No Hit
AAACGAGGGGTGGATTTTTGAAAATAAATATATTGGAAATATTTTTTGAGAT2130.10968921389396709No Hit
AAATCGAGGGGACTTGGTAAGATGCTCTGTTACAAATTGCCACTGCACACTG2130.10968921389396709No Hit
CTGTAAGGGTTCCCGGTGCCTGCTGTTGGAGTCAGCATAGCATATGATTATG2130.10968921389396709No Hit
AGCCCAGACCACTCTGAACTCCAGATTCACATACCCAGCTGCCTACTGGGCT2130.10968921389396709No Hit
TTCACTGGGGGAGATGCCTAAGCTTTTATGAGACTTTACATAAAATCCTAAA2120.10917424105878415No Hit
CTTAGCGGGCGGAAAATCCTGAATGTGAAGAGATCTCAGGGGTCATCGCCTC2120.10917424105878415No Hit
CGACCAGGGTATGGTGAAAATATTTATCTGGAATTTATGTTGTTTAGTGTTA2120.10917424105878415No Hit
TCTTCTGGGGTGGCTTGAACTTGCCAGATGCAAATACCACAGACTCCAAGAA2120.10917424105878415No Hit
CATTATGGGCACCAATTGGCCAGCAAAACCTTTTGTTCACATAACATATTAA2100.10814429538841826No Hit
AAGCCTGGGGATGACCCACCAATCACATGCCTATCATATAGTAAAACCCAGC2090.1076293225532353No Hit
TGAAATGGGCACTTCTGTTATTTCCCTTTTAACCCTTGCCCGCCTATGTTTT2090.1076293225532353No Hit
GACTTAGGGCACTGTAGAAAAACTGAATGAAAGCACGCATCAGTAGTGACTG2090.1076293225532353No Hit
TGTGTTGGGTATCCTACCAGGCTTCGGAATAATCTCCCATATTGTAACTTAC2080.10711434971805237No Hit
TTAAGAGGGGCCTTTTCTTCCTTTTGGTGCGAGCTTGCTGTGGTTTTTGCTC2080.10711434971805237No Hit
ATGACAGGGAGCACTGAAACCTGAAGTGTCTGTGATATGTATAATAAGACAG2080.10711434971805237No Hit
GTACTGGGGCTATTAAAGTGGCTGTGTTCCCTGCTAAATTAATGTTTAAGGG2080.10711434971805237No Hit
AGCACAGGGATGAGGAAAATGTATACTATATTGTGATAAAATTACATTTCAT2080.10711434971805237No Hit
TTCAATGGGACAACAATCAGTCTTCAAATTTTGGACCCATGAAGGGAGGAAA2070.10659937688286944No Hit
GGCAGGGGACTAATGATATTGAGTATCTTCTCATGTGCTTACTTGCCATCCG2070.10659937688286944No Hit
CTAAGTGGGTGCAGGGAGCCGATACGTTTGCTGATAGCAATACTGGAACCAC2060.10608440404768649No Hit
CAATTGGGGGTGAGTTCTATCTTTTTCTTTATAAACTGTCCATATTGTGCTC2060.10608440404768649No Hit
GGCACTGGGACTCAAGGCTTATCTCCAAATAACCCTTCCTAACTCACCTAGC2060.10608440404768649No Hit
GTACTGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA2060.10608440404768649No Hit
CCACCAGGATGGTACATTTATTAAAATGAATTGACCTGCATTGACACATCTT2050.10556943121250355No Hit
TATTAGGGGCCTTCGATGCTGCTGCCCACCAGTGATGTAAATCACAAAGCAT2050.10556943121250355No Hit
TAGTAAGGGGCGCTCTTCCGATCTTGCAAAGGGAATCATTGGAAAGTGTTAT2040.10505445837732061No Hit
AAGCCCGGGAATGTTCTTCAAAAAAGTAATATCCTCACTTGGAGAGTGTCAA2040.10505445837732061No Hit
AATGCGGGGTCTTAAGTCTGTGGCACACTGTGTCTTCAGACAGTTTGAAGGA2040.10505445837732061No Hit
TGTCCAGGGTATTTTCAGCAGTAGAAATTCTTCGATGGCCCATTTCAGACCA2030.10453948554213766No Hit
GTAACAGGGTATTAAACAGATGCAGTCATCATTTACATCTTCAGAACAAGAG2020.10402451270695472No Hit
GTAAAAGGGAGAACACAGCCAGCTAGTAAGGTTTTCCCACTGGGGCTGACAT2020.10402451270695472No Hit
CATCAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA2010.10350953987177176No Hit
GCCAGAGGGGCTTTGTTAGGCTGTGTGATGTTCATACATTTTCTCCCCATCT2000.10299456703658882No Hit
GCCACTGGGGGGGCATTTTTATATAATCAACAACTAAATCTTTTGCCAAATG2000.10299456703658882No Hit
AATACAGGGCACAGTTTGATGCTTTTAATTTTCTGTTATATAAAACACAATG1990.10247959420140587No Hit
TTTGACGGGATCATTTTTATTTGCTAGCCTGTTAATATTATTCATTATATAA1990.10247959420140587No Hit
CATTTTGGGAAAGATTCAATGATACAATTATCAGTGATTGCTTATATATTAG1990.10247959420140587No Hit
GCTAGTGGGGTGAGAGCTTCTAATATCTAAATTAATGTTGAATCATTATTCA1980.10196462136622293No Hit
GCACTCGGGATCCAGTGCTAGTGGCCAGACTCTTCATTCCAGAGTTATTCAC1980.10196462136622293No Hit
GTAGAGGGGTGTGCTGGCTGGACACCCTCTGCTGGCAGCATCTTCATGGGCA1970.10144964853104No Hit
TATGCAGGGGAGTTCCTCCTAAGGAAATTGAGGAGAGGGACTGAATAGAAGC1970.10144964853104No Hit
ACTTATGGGGAATATTTCAAGTTTCAAACTACTTTTACCATGTTATATCTAT1970.10144964853104No Hit
GGCAATGGGTGAAAAACAATTTATGTTTGCAGGGTTTAAAAATCAGTAAAAA1960.10093467569585704No Hit
CTCTTTGGGAGAACTTCAGGGGCTAAGTATGCTGGAGTTCCTACCACAGATC1960.10093467569585704No Hit
CTAAACGGGGGGCACTGTGATTGTGCCTGTGAATAGTCACTGTACTTCAGTG1960.10093467569585704No Hit
GGTACGGGGAGGAAGCTGAAGAAAAAGCGCCAGTGAAGAAATCTATACGAGA1960.10093467569585704No Hit
TTCTTAGGGAGAAATCTATCAAAATATAACAAATACTTTCATAATGCGAAAT1950.1004197028606741No Hit
TTTTCAGGGACTCTCGGCCTATCATGTTCAATTAAGCTAAGGATTTAAAAGT1950.1004197028606741No Hit
GCAAAAGGGGGACCGCTTTCCGGGAGACTGGAGTCGAAGGCCGTGAGGTATT1950.1004197028606741No Hit
ACATCGCGGGGATCTCTGAATGGGTGAGGATTAATCTGATTTATAAGTAATA1950.1004197028606741No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)