FastQCFastQC Report
Sat 31 Dec 2022
SRR5846936.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5846936.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences191078
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACGCTGAGGTATCTCGTATGC5380.28156040988496844No Hit
CTTATACACATCTCCGAGCCCACGAGACGCTGAGGTATCTCGTATGCCGTCT5210.27266351960979285TruSeq Adapter, Index 2 (95% over 21bp)
CGGCTAGGGTATTTGCAGACTATTCATCCTGTGAGGGGCTAGGATCCAGAAT2990.15648059954573523No Hit
ATTCATGGGGTAACTGACAAGATGAATTACTCAGTTTCTCTTTCTCTAAAGC2510.1313599681805336No Hit
AAACGAGGGGTGGATTTTTGAAAATAAATATATTGGAAATATTTTTTGAGAT2500.13083662169375856No Hit
CGTATGGGGAAGTAAGAAAAGTTTAGATTAGAGCAAGTAAGCGAAAAATCAC2500.13083662169375856No Hit
AAAAACGGGATTCCTGATCGAGGCTTGCAAAGTGAATCCTTTTGCCAAGGAC2490.13031327520698352No Hit
AGATTGGGGCCTGACAAAACACATAGCCTACCCCTTCCTTGTACTATCCCTA2490.13031327520698352No Hit
CAACAAGGGTATGAGCACCTGGAATACAATCAGTGGAGATATTTATTTGAAT2380.12455646385245815No Hit
AATTGTGGGCTTCTACATGTGGGTATCCTTTTTTCCCAGCACAATTTATTGA2360.12350977087890809No Hit
GACACCGGGGTATCCCAGAATGTGTTGTGTTTTTTTAAAAGATGCATAATAG2360.12350977087890809No Hit
ACAGTTGGGGATCTAACACCGGGATGAGAACAAAGGACAATGTACTAGAATC2330.12193973141858298No Hit
ATTTCCGGGGGAGAGAAGGAAAGGAGAGAAGGCAGGAAGAAGGCAAGGGACG2290.11984634547148286No Hit
AGCAAAGGGCACAGATCCAATTTCTGAACAAAACATATCAAAAATTGAAGGT2270.11879965249793278No Hit
TGAGATGGGGGTCACAGTAAGCACCCAGTGAATGTTTGTTGACTATCGAATG2260.11827630601115775No Hit
TATAAAGGGCTCCCAGGTTCAAGTGATTCTCTTGCCTCAGCTTCCCGAGTAG2240.11722961303760769No Hit
CTGACAGGGAAGACAGAGCTTTAGATTGCAAATCAGTTTAGCCTCATCAGTT2240.11722961303760769No Hit
GCGTGGGGATCATTCTGAGGGGCCACAGTAATTACAAACTTACTATCCGCCA2230.11670626655083263No Hit
AGCAATGGGCTCCCACGTCAGCCTCCTGAGTGGCTGAAACTGCGGCTGCGCA2200.11513622709050754No Hit
TTTTCTGGGATGACAGAAAGAAAGAGGGAGTGGTGGAAAGAAACAATAGGAT2180.11408953411695746No Hit
TCAAGTGGGAAGTATAACATTTTTCCTTTGTAGATTGGAGAAAGAAGTCATG2180.11408953411695746No Hit
CAGTGCGGGGCTCTTTTTCCGGCTGGAACCATGGAGGGTGTAGAAGAGAAGA2170.11356618763018243No Hit
CGATATGGGAAGGCACATTGATCCATTTACTCATTCATTCATTCATTCATTC2170.11356618763018243No Hit
AAGCCCGGGAATGTTCTTCAAAAAAGTAATATCCTCACTTGGAGAGTGTCAA2170.11356618763018243No Hit
GCATCAGGGGGCATTTACTGGCTTATGATGTCTGAGGCATTGATCTAAGGAC2160.1130428411434074No Hit
TAGAAGGGGGATGCTGATTGTTTGGGTCACTTCATTATGTTGACAAATTCCT2160.1130428411434074No Hit
TGTCCAGGGTATTTTCAGCAGTAGAAATTCTTCGATGGCCCATTTCAGACCA2160.1130428411434074No Hit
GTAACAGGGGTAGCAGGTTAAATAACAGTATCCTCTAAGGAGCTGAGTTCAG2160.1130428411434074No Hit
AACAATGGGGGCGTAGGAGCGTGCGTTCGGGCCCTCTTCTCCCACCTGTTCG2150.11251949465663237No Hit
TACAAAGGGATGTGCAAATTTTAAAGAGTCGTAGCCAGGTCAGTCGGGTCTT2140.11199614816985734No Hit
AAAAGCGGGGCAAGGTGACCATTTTAAGCAAGAAATTGATCTGACATACGTA2140.11199614816985734No Hit
CATAAGGGGTCTTGTATAAATAGGAGTCATGGGCGTTAGTTCTGTAGTAATA2140.11199614816985734No Hit
GGCATAGGGACTGAGCAAATGTTTGAAATTATGTTAATTTTGACAGGTACAA2140.11199614816985734No Hit
GCACTCGGGATCCAGTGCTAGTGGCCAGACTCTTCATTCCAGAGTTATTCAC2130.1114728016830823No Hit
CTAAGTGGGTGCAGGGAGCCGATACGTTTGCTGATAGCAATACTGGAACCAC2120.11094945519630726No Hit
ATCGACGGGAGTTCACACGTGGCGCCAGCGGAGGCAGGTTGATGTGTTTGTG2120.11094945519630726No Hit
TAGTAAGGGGCGCTCTTCCGATCTTGCAAAGGGAATCATTGGAAAGTGTTAT2110.11042610870953223No Hit
CTCTGTGGGCTTTTTGTAAATGCGTCCTGATAATGATTAGGAAAATCGACCT2090.10937941573598217No Hit
CCACCAGGATGGTACATTTATTAAAATGAATTGACCTGCATTGACACATCTT2080.10885606924920713No Hit
TCACAGGGTGAAACGTGATGAACGCACACTGAAAAACACAGATATCAAAGTT2080.10885606924920713No Hit
TTCACTGGGGGAGATGCCTAAGCTTTTATGAGACTTTACATAAAATCCTAAA2070.1083327227624321No Hit
AAACTTGGGGAATGCTGATGATATTTTATTAGAATGAAAGCACAGATTAGCA2070.1083327227624321No Hit
AACAGCGGGAATAAATAAAAGTAAAAATGTGTTATCTAATTTCTGTGAAGCA2070.1083327227624321No Hit
CACCAGGGGGTATATTAAAATTTACTGCACACTTTGAGATTGCTGCCATTTT2070.1083327227624321No Hit
TGACCCGGGTTCTCCATCCTGCAAGCCACTCCTTTTACCTCCACTGTAACTC2060.10780937627565707No Hit
CAAATGGGGTACGCTGCTGCACAAGTGGGCGAGCGGCAACTTCCGTCATGAG2060.10780937627565707No Hit
CCAGCCTGGGTAACAGAGTGAGATTCCATCTCAAAATAAAATAAAATAAAAT2060.10780937627565707No Hit
CGACGAGGGCAGTAGCAATACTTTTAATTGTCTTATGCTTTTCAACCTATAG2050.10728602978888203No Hit
TCATTAGGGAAGGCAGTCATTGTCCTGGTGGAAAACTTCTACAAATATGTGT2040.106762683302107No Hit
AGTACTGGGCGTTACTAAGTAGAATAGGTGGACCTAACATTATTTGTAGATG2030.10623933681533194No Hit
GACTACGGGGAGGTGAAGGGATGCCGCAGTATCGTAATCCCTTTGAAAAAGG2020.10571599032855691No Hit
TATTAGGGGCCTTCGATGCTGCTGCCCACCAGTGATGTAAATCACAAAGCAT2020.10571599032855691No Hit
TGAGTAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA2010.10519264384178188No Hit
TGCAATTGGGCATGGAGGAAGAGAATATTAGGTTAATTATCTTAATGATATT2010.10519264384178188No Hit
TTTGACGGGATCATTTTTATTTGCTAGCCTGTTAATATTATTCATTATATAA2010.10519264384178188No Hit
TACTATGGGTGAGCGGTTGAATTGGGAGAATGAAGATAAGTAATTTACCTGT2010.10519264384178188No Hit
AGCACAGGGATGAGGAAAATGTATACTATATTGTGATAAAATTACATTTCAT2010.10519264384178188No Hit
TGTCTTGGGGAAATCTCATATTTGTAAAATAGGCATTAGAAGATAATTGTGA2000.10466929735500685No Hit
TGAACAGGGGTAAAATTAAAGAGAATTGTCTAACTATTTTGACTCATTCTGC2000.10466929735500685No Hit
TTGCTGGGAGATATTTTTACTAAATTAAATGAAGTTAATTTGTCAATGCAAG2000.10466929735500685No Hit
ACGTATGGGAACATAATGTCCTTGGGAAATGATGATAGCCCAATGAACTTAA2000.10466929735500685No Hit
TCCTGGGATTGCAAGAGGCGAGTTTAAAATTCTGCAGATGGCCTTATTTGTG1990.10414595086823183No Hit
ACACTCGGGAGAAGAACAAACTAAGTCCAAAGTCAGCAGGAAGAAGGAAATA1990.10414595086823183No Hit
AGCCCAGACCACTCTGAACTCCAGATTCACATACCCAGCTGCCTACTGGGCT1990.10414595086823183No Hit
GGCACTGGGACTCAAGGCTTATCTCCAAATAACCCTTCCTAACTCACCTAGC1980.1036226043814568No Hit
AATCGCGGGCAGTGTTTGATTTTTTTCATTAGTTGAAGTGAGTTTTAGTTTT1980.1036226043814568No Hit
GTATCAGGGATATTGTGAAAATGACCATAATGCCAAAAGCAATCTACAAATT1980.1036226043814568No Hit
GCTGTGGGGGCCGGTTGACTTTGCGGAGCCATGGAGGGCGGCTTCGGCTCCG1970.10309925789468175No Hit
AACCGAGGGAGTCTGCAAACATTACCAAATGTTTCTTGGGGGACAAAAGCAC1970.10309925789468175No Hit
AAGCCTGGGGATGACCCACCAATCACATGCCTATCATATAGTAAAACCCAGC1960.10257591140790671No Hit
TTAACTGGGCAAGCTGAAACGACATGCCAAGGCCCACGAGGGCTATGTATGT1960.10257591140790671No Hit
ACTAAAGGGCAGGAAATTTTAACCAATTCTATTTGGAAAAAATTTGTTTTTG1950.10205256492113168No Hit
CTGCGAGGGAAATCAAAACCACTATGAGATATCATCTCACACCAGTTAGAAT1930.10100587194758162No Hit
CTGTAAGGGTTCCCGGTGCCTGCTGTTGGAGTCAGCATAGCATATGATTATG1930.10100587194758162No Hit
TGTGTTGGGTATCCTACCAGGCTTCGGAATAATCTCCCATATTGTAACTTAC1920.10048252546080658No Hit
TTCTAGGGGACCAGACACAGAGAAAAGGGAAATTGTGTTTTATTGAGCGATT1920.10048252546080658No Hit
GGTGCGGGACGAGAACGACTGCATTTTCGACAAGTTTGAATGTGCCTGGAAC1920.10048252546080658No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)