FastQCFastQC Report
Sat 31 Dec 2022
SRR5846929.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5846929.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences113069
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACGCTGAGGTATCTCGTATGC4620.40860005837143687No Hit
CTTATACACATCTCCGAGCCCACGAGACGCTGAGGTATCTCGTATGCCGTCT4570.4041779798176335TruSeq Adapter, Index 2 (95% over 21bp)
TTAACCGGGGTTTCCAAATTGTTAAAATGAGTCCCTATCAATCATCAAATGT1250.11055196384508574No Hit
TATCTTGGGTGAGGTGGCAGAAGTGAGGAAAGCATTGGAAATAAAAAATATT1220.10789871671280368No Hit
AACACGGGAACCAGTTTAGAGAAGAAGATAAATGACCTGATGGAGCTGAAAA1200.10612988529128231No Hit
GTATCTGGGAGTGTGGAGTAGAGAAACGAGGCATGGGAAGAATGGGGTGGAA1180.10436105386976094No Hit
ACTAACGGGCGCTCTTCCGATCTTGATTTGGGGAGGCATTGAGGCAGCCAGC1140.10082339102671821No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)