FastQCFastQC Report
Sat 31 Dec 2022
SRR5846928.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5846928.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences113606
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACGCTGAGGTATCTCGTATGC4170.3670580779184198No Hit
CTTATACACATCTCCGAGCCCACGAGACGCTGAGGTATCTCGTATGCCGTCT4030.354734785134588TruSeq Adapter, Index 2 (95% over 21bp)
ACTAACGGGCGCTCTTCCGATCTTGATTTGGGGAGGCATTGAGGCAGCCAGC1280.11267010545217683No Hit
AACACGGGAACCAGTTTAGAGAAGAAGATAAATGACCTGATGGAGCTGAAAA1220.10738869425910604No Hit
TTAACCGGGGTTTCCAAATTGTTAAAATGAGTCCCTATCAATCATCAAATGT1200.10562822386141577No Hit
ACAAGTGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1180.10386775346372551No Hit
ATCCCAGGGATCGATTGGAACCCTGATTGGCGGCGTCTACCCCGGGAGCTAA1160.10210728306603524No Hit
CTAGTATCGGGGGGATGGAATGGAAAGGAGTGGAATGGAATGGAATGGTATG1140.10034681266834497No Hit
GGTTGCAGAAATTTAAGAAAAGACATGGCATTAAATTTTTAAAGACTTGTGG1140.10034681266834497No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)