FastQCFastQC Report
Sat 31 Dec 2022
SRR5846927.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5846927.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences110930
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGCTGAGGTATCTCGTATGCCGTCT3860.3479671865140178TruSeq Adapter, Index 2 (95% over 21bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACGCTGAGGTATCTCGTATGC3760.3389524925628775No Hit
AACACGGGAACCAGTTTAGAGAAGAAGATAAATGACCTGATGGAGCTGAAAA1220.10997926620391239No Hit
TATCTTGGGTGAGGTGGCAGAAGTGAGGAAAGCATTGGAAATAAAAAATATT1200.10817632741368431No Hit
ACAAGTGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1150.10366898043811412No Hit
TAGAGTGGGGGCGATGGCGGCGGTGGCTGCGTTGCAGCTGGGGCTGCGGGCG1130.10186604164788605No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)