FastQCFastQC Report
Sat 31 Dec 2022
SRR5846908.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5846908.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences130958
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAAGGAGCTATCTCGTATGCCGTCT8810.6727347699262359RNA PCR Primer, Index 38 (95% over 21bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACAAGGAGCTATCTCGTATGC7970.6085920676858229No Hit
TCTCTCGGGATGAGTAATTTATTCTATTGTCCTATAAATATTAATTGAGCAG2010.15348432321813102No Hit
GATCTCGGGGGCACTCGCAGGCGACCTGCTCCAGTCTCCAAAGCCGATGGCA1790.1366850440599276No Hit
GTGAACGGGAAGTAAAAAATGTGAAGAGAAAAAAATTGCAAACAGAAGAATA1750.13363062966752698No Hit
GGTCACGGGGAGACGACCAGGGCCGGCGAGCGCAGTGCAGCGCCGTGCGGTG1750.13363062966752698No Hit
GCTGTAGGGGGAGGCCACCGCTGCCCTGCCATCCCGTCTGCCTGCCATGGGA1700.12981261167702624No Hit
ATCACCGGGCTGGCATTTTCTCTCCTGAGAACAAGGTAGTGGTTCCCAATTG1690.12904900807892608No Hit
CATTCCGGGAGTTGTCTAGTGTAAGCAGAAGCTGATAAATGCTTGTTGCTGA1670.12752180088272574No Hit
GCAACAGGGATTGTGACACTGTGAACATTGGATAATTATAAAAAATAGTGCT1650.12599459368652546No Hit
CGTTGCGGGGAAACTGAATTAACTTGGGAATATTTGTTGTTAAAAACTTCTT1640.12523099008842528No Hit
TATACTGGGTAAAAAATACATTGTCCCTTTACACATATGGTTTCTCCTATCA1620.12370378289222499No Hit
AACCCGGCACATTAGTTTGAAGAGCAAAACTGGAGTACACCTTCCTGAGCCA1600.12217657569602468No Hit
TGCTATGGGCTTGGGGCCTCCTAAGAAAATGTTTTAATAAGGAAGTCCAAGG1580.12064936849982438No Hit
TGGCAGGGGCGATCTTATACGGGTTATCTGCCTCTTCCTACACATCGGGCGA1580.12064936849982438No Hit
AATGCGTGAAAAGAAAAAACTGAAGAAAAAACTTGTCAGGTCTGAAGAAAAC1560.11912216130362406No Hit
GCAAACGGGGTAAATAAACTGTGGAAAAGATACTTGTTTACAGTATGAGAGC1530.1168313505093236No Hit
TAGCTTGGGTGTCAAAAGCTGGGATGGTGATGCCTAAATCCGCATGACAGTT1530.1168313505093236No Hit
AATAGTGGGATGCTGTTTCAGAGTCAGGGTTCAAGGAAATGTCTTAATGACT1520.11606774691122344No Hit
TATGTCGGGCTCTAGTTATTTCTAAACAAAATTTTACTTCTTGATGACTACA1500.11454053971502312No Hit
TGTACCGGGAGCTTAAGAGACTTATGGGACACCATCAAGTGACTAATATGTA1500.11454053971502312No Hit
GCTACTGGGCTCACATCTATAAGCGAGACAGTTTTGCAAATAAATTCATAAA1500.11454053971502312No Hit
GCAAAGGGAACTGTACAAAAAAATGCTTCTGGAGATTTCTTTGGCAGAAATG1490.11377693611692298No Hit
CTAAGCGGGGACTTGACCATCTTTCCAACCCCTGGGGAAGACATTTGCAACT1490.11377693611692298No Hit
GAGCTGGGACCTCGGAGAGGGGGAGGCCTCGCGCCGGTGGCTACAGCAAGAG1490.11377693611692298No Hit
GCTAAAGGGGGGCTGGCCTGGCATTAGTCATTATTTAGTTTCCAGGCCAACC1490.11377693611692298No Hit
GCGGTAGGGAGACACAAGGCTGCTAAACCAATTAAGAAAATAATTAATTTCT1480.11301333251882283No Hit
GAAAAAGGGAGGATACTGATTTTAGCTTTTTACTAGGTCCTGACATTCCCGT1480.11301333251882283No Hit
GCCACGGGAAACTGAGTTTTTCTTATATTCACAATGGTTTTAATTTTTCTCC1480.11301333251882283No Hit
AAGCACGGGAGCTGAAAATGATTTGGAAGAAACAATTTCATTGCATTGCCAA1470.11224972892072269No Hit
GCACGAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1470.11224972892072269No Hit
TAATGAGGGATCCTCCCACCTGGAGGGTGTGAATGGGGATCAGTTTTTCAAT1460.11148612532262252No Hit
TGATTAGGGTGTCAAAACCAAATGATGATGTATTTTCCTTAAGGCATTGTTT1460.11148612532262252No Hit
AAATTAGGGTGGTATTCATTAGATTCCTGAAGGGATGTATCTATAAGATTAA1460.11148612532262252No Hit
GAGAAAGGGAAGCATAACCCTGAGATTCTTACTACTGATGAGAACATTATCT1460.11148612532262252No Hit
TACGCTGGGATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTA1450.11072252172452236No Hit
TACTGTGGGATCATATTTCAAAAACTTCCTATTTCTTGTGTTATGAACCACC1440.10995891812642222No Hit
ATCCGGGGATCACCATCAGTGTTGTTTTGCCAGTGTTGTTCAGATTAGCTGT1440.10995891812642222No Hit
TCTGCAGGGGTGTCTTCTCTTCATGAGACACATTAATTGGTAAAACTCAAAT1440.10995891812642222No Hit
TTCATCGGGAGAGGAAAGTTGGAAAGGGGCGGTAGTCAAGGAATATAGAAGT1440.10995891812642222No Hit
AAGCGAAGGGGAGTCAGGCCTGGGCAGGACCCTGCTGACTCGTGGCGCGGGA1430.10919531452832207No Hit
TTAATTGGGTGATGGCCATTTAAATAGAATTTTATTCTATCTAATTCATTTA1430.10919531452832207No Hit
TGATTTGGGGAGTCGGAGCCAGCGCGAGCCGCCGCCGCCATCACTGCCGCTG1430.10919531452832207No Hit
TAGCTCGGGCATTAAATATGTGTTAATTGACTGTGTTATTTGTCAGGCTTAC1420.1084317109302219No Hit
GTCTGCGGGAAGACCAGCGTGGCCAACGTGGTGAAACCCTGTCTCTACTAAA1420.1084317109302219No Hit
TGGCATGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1410.10766810733212175No Hit
ATATTAGGGACTACTGCACCCACTACCACGCTTGGCTAGTTTTGTATTTTTG1410.10766810733212175No Hit
AGATGTGGGAACATTCTTCTAGCTACATTTGTTGAAAATAAATATTAATCTT1410.10766810733212175No Hit
TCGACTAGGGGCTTGAAAATGATTCTCTTAATTACAGTCGTGGAGTTGCTTT1410.10766810733212175No Hit
ACGTCGGGCTGGCGCAATAGATATAGTACCGCAAGGGAAAGATGAAAAATTA1400.1069045037340216No Hit
TCAATAGGGCTGACCTATTTTAGAAACACTTATTTAATAGTATAATCAAAAA1400.1069045037340216No Hit
TCGCGCAGGGGGCCCTTGCAGGCAAGAGTGCTGGAGGGCGGCAGCGGCGACC1400.1069045037340216No Hit
GGCGCTGGGGGCCAATGAAATAGCAGAAGTGATGTGCACCACATCTTAGCAC1380.10537729653782128No Hit
TAAAGACGGGATGCCTGTGCCGCCGATGATGACTTTGACACGGATTTTGACC1380.10537729653782128No Hit
AGTGCTGGGATCCTCAAATAAGATTAACAGCTGTTTTTCATCAGAAACCATG1380.10537729653782128No Hit
CTCGTTGGGATCTATATTTTGAACTATTGGCCAGTTACTCTGAGTTAAAGAA1380.10537729653782128No Hit
TTCTCGGGGAACTCTTCGTGGGCATGCTCAACAAGCAACAGTCCGAGGACGA1380.10537729653782128No Hit
CACCATGGGAATCTTTAATTCACTGAAGTCTATGATTATTCTTCACTTTTTT1370.10461369293972113No Hit
GTAACGGGGAAGTATTTGATACAGACTTAATATGAGGTGGATGCAAATATCA1360.10385008934162099No Hit
CTGCTGGGGGAGTCATGGCAGAGCTCTCGGAGCCGCTCAGCTGGGCTCCAGA1360.10385008934162099No Hit
ACCTCCGGGATTTTATAAAATTATGAAATCAGGAAGTTAACATCTTGGTTTT1360.10385008934162099No Hit
GAAGCTGGGATCACTTAAAATTGAATTCATCCATTTTTAAAAAACACTTCAT1350.10308648574352082No Hit
CACGCAGGGGTACTAAATTGACTATAAATTGAGTCTGCAAAGAGGAAACTTT1350.10308648574352082No Hit
AGGGGCGGAGAAAAAGAAAGAAAACCATTCTCTATTTTTCCATGGGTATGAT1350.10308648574352082No Hit
CGTACGGGGATTATCGGGTGTCTCAGTCCATCACTTCCATTCTCTGAATCAC1340.10232288214542067No Hit
CATCAGGGAGAAGAGAAAGGTCCTGGAGGCTCCAAGTGGGGCCCTCTCCCTC1340.10232288214542067No Hit
TGTTAGGGGATACATGCTGTTTTTTAATTAAAATATAATCACTGAAGTTTAC1330.10155927854732053No Hit
AGGCGGAGCTTGAGGAAACCGCAGATAAGTTTTTTTCTCTTTGAAAGATAGA1330.10155927854732053No Hit
GATACCGGGACCCGCCCCAAGCCAGGGGAGCTGGCCTTCCGCAAGGGCGACG1330.10155927854732053No Hit
CTACAACGGGAATGTAACATTTTTAAACATAAACAACTTTAGATTCTGTGAA1330.10155927854732053No Hit
CTGAAAGGGATACTTGGTTTTGGTCATTCTTTTTTCATCCTGCGAACATTTA1330.10155927854732053No Hit
ACCGTAGGGACATCTTGAAAGATGTATTTTTCCAAAAGATGCCACTTTTGCC1320.10079567494922034No Hit
AGTTATGGGCTCATACTCTTCTGCTAAAAGGTATAAATGTTCAACTTTTTTT1320.10079567494922034No Hit
CCTGACCTTGTGATCCGCCCGCCTCAGCCCCTCAAAGTACTGGGATTACAGG1310.1000320713511202No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)