FastQCFastQC Report
Sat 31 Dec 2022
SRR5846907.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5846907.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences129822
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAAGGAGCTATCTCGTATGCCGTCT9380.722527768791114RNA PCR Primer, Index 38 (95% over 21bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACAAGGAGCTATCTCGTATGC7970.6139175178321086No Hit
GTGAACGGGAAGTAAAAAATGTGAAGAGAAAAAAATTGCAAACAGAAGAATA1990.15328680809107856No Hit
GATCTCGGGGGCACTCGCAGGCGACCTGCTCCAGTCTCCAAAGCCGATGGCA1970.15174623715548982No Hit
GCTGTAGGGGGAGGCCACCGCTGCCCTGCCATCCCGTCTGCCTGCCATGGGA1930.14866509528431235No Hit
TCTCTCGGGATGAGTAATTTATTCTATTGTCCTATAAATATTAATTGAGCAG1880.14481366794534054No Hit
TGTACCGGGAGCTTAAGAGACTTATGGGACACCATCAAGTGACTAATATGTA1860.1432730970097518No Hit
CCTGACCTTGTGATCCGCCCGCCTCAGCCCCTCAAAGTACTGGGATTACAGG1800.13865138420298562No Hit
TGCTATGGGCTTGGGGCCTCCTAAGAAAATGTTTTAATAAGGAAGTCCAAGG1750.1347999568640138No Hit
CATTCCGGGAGTTGTCTAGTGTAAGCAGAAGCTGATAAATGCTTGTTGCTGA1720.1324891004606307No Hit
CGTTGCGGGGAAACTGAATTAACTTGGGAATATTTGTTGTTAAAAACTTCTT1670.12863767312165889No Hit
TTGCTCGGGAAGACTTCTCGGATCTGGTCCTTGGAAGGAAAGTCAATAATGA1670.12863767312165889No Hit
TCTGCAGGGGTGTCTTCTCTTCATGAGACACATTAATTGGTAAAACTCAAAT1620.12478624578268706No Hit
AATGCGTGAAAAGAAAAAACTGAAGAAAAAACTTGTCAGGTCTGAAGAAAAC1620.12478624578268706No Hit
GGCGCTGGGGGCCAATGAAATAGCAGAAGTGATGTGCACCACATCTTAGCAC1610.1240159603148927No Hit
AACCCGGCACATTAGTTTGAAGAGCAAAACTGGAGTACACCTTCCTGAGCCA1590.12247538937930397No Hit
ACTATAGGGGGGACTGGAGAAGGGAGGCGGCGGGCGAAGCGCACGTCGAGCG1590.12247538937930397No Hit
GCTAAAGGGGGGCTGGCCTGGCATTAGTCATTATTTAGTTTCCAGGCCAACC1570.12093481844371524No Hit
GCATATGGGAAACTTGGTGTTCATCAGTAGATAAATAGATGAACAAAATGTT1560.12016453297592088No Hit
GCAACAGGGATTGTGACACTGTGAACATTGGATAATTATAAAAAATAGTGCT1550.11939424750812651No Hit
CCATGGGGGGAAGTCATTTGCCCATTTAATTTTTTTTCTTTTTCTTTCTTTT1550.11939424750812651No Hit
TACGCTGGGATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTA1550.11939424750812651No Hit
CTACAACGGGAATGTAACATTTTTAAACATAAACAACTTTAGATTCTGTGAA1520.11708339110474342No Hit
GCTTGAGGGGGAAGCTGTGCTGTGTGTGTAGACAGGGTGCTGTACTTGTTTG1510.11631310563694905No Hit
GCAAACGGGGTAAATAAACTGTGGAAAAGATACTTGTTTACAGTATGAGAGC1510.11631310563694905No Hit
TTAATTGGGTGATGGCCATTTAAATAGAATTTTATTCTATCTAATTCATTTA1510.11631310563694905No Hit
TTGCCCGGGAGTGAGCAAATCGCGGACCACCGGGGCTGCCAGCTCGCCTGAC1510.11631310563694905No Hit
TGGCAGGGGCGATCTTATACGGGTTATCTGCCTCTTCCTACACATCGGGCGA1510.11631310563694905No Hit
AGTGCTGGGATCCTCAAATAAGATTAACAGCTGTTTTTCATCAGAAACCATG1480.11400224923356596No Hit
TACTGTGGGATCATATTTCAAAAACTTCCTATTTCTTGTGTTATGAACCACC1470.1132319637657716No Hit
AGATCGGGGATTGTAAGCTAAGCATTAGGACTGATACAAATAATATATGCTC1470.1132319637657716No Hit
GTGTAGCGGGACATAAAGCAAGTGAGCGACCTTATCTCTGTGCTTCGTGAAG1470.1132319637657716No Hit
GCAAAGGGAACTGTACAAAAAAATGCTTCTGGAGATTTCTTTGGCAGAAATG1470.1132319637657716No Hit
GCACGAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1470.1132319637657716No Hit
ATCACCGGGCTGGCATTTTCTCTCCTGAGAACAAGGTAGTGGTTCCCAATTG1460.11246167829797724No Hit
AAGCACGGGAGCTGAAAATGATTTGGAAGAAACAATTTCATTGCATTGCCAA1460.11246167829797724No Hit
TACGAAGGGTTATCCTCAGCAGTATAGTGGGATTGTCAAGATTATTATATTT1450.11169139283018287No Hit
GGTCACGGGGAGACGACCAGGGCCGGCGAGCGCAGTGCAGCGCCGTGCGGTG1450.11169139283018287No Hit
TATACTGGGTAAAAAATACATTGTCCCTTTACACATATGGTTTCTCCTATCA1450.11169139283018287No Hit
GAGCTGGGACCTCGGAGAGGGGGAGGCCTCGCGCCGGTGGCTACAGCAAGAG1440.11092110736238849No Hit
TGATTAGGGTGTCAAAACCAAATGATGATGTATTTTCCTTAAGGCATTGTTT1430.11015082189459414No Hit
ATCCGGGGATCACCATCAGTGTTGTTTTGCCAGTGTTGTTCAGATTAGCTGT1430.11015082189459414No Hit
AATAGTGGGATGCTGTTTCAGAGTCAGGGTTCAAGGAAATGTCTTAATGACT1430.11015082189459414No Hit
CACTGTGGGAAATAACTTGCCGGGCGGTGGCTCACCCCTGTAATCCCAGCAC1420.10938053642679978No Hit
GCTACTGGGCTCACATCTATAAGCGAGACAGTTTTGCAAATAAATTCATAAA1420.10938053642679978No Hit
AAAGCTGGGATGTGAAGGCCTGTTTTAAGGAAAGGGAAATTGGAAGAACTCA1420.10938053642679978No Hit
CATGTTGGGGAGAGCTTTTCTGTGTTTCTCCGGACTTCGAGCCATGGCGGTG1410.1086102509590054No Hit
GATACCGGGACCCGCCCCAAGCCAGGGGAGCTGGCCTTCCGCAAGGGCGACG1410.1086102509590054No Hit
CTGAAAGGGATACTTGGTTTTGGTCATTCTTTTTTCATCCTGCGAACATTTA1410.1086102509590054No Hit
CTAAGCGGGAGAGATAATCTGTCTCTCCCATCAGCCCCTGAACAATTGATAG1400.10783996549121105No Hit
TAATGAGGGATCCTCCCACCTGGAGGGTGTGAATGGGGATCAGTTTTTCAAT1390.10706968002341669No Hit
CACCATGGGGATTGGTGGTAGGACTAAGAAGGAAACAGATATTTTAAATACT1390.10706968002341669No Hit
TGAGAAGGGTCTAAGCACGGGGTGCTGGAAGCTGTGGCATGGCTAGCAGGAG1390.10706968002341669No Hit
TCGCGCAGGGGGCCCTTGCAGGCAAGAGTGCTGGAGGGCGGCAGCGGCGACC1390.10706968002341669No Hit
ATCAGCGGGATTGTGTGCTTAGTCGTATTACTCAAATGAGTGAAATTAGCTT1370.10552910908782796No Hit
ATCTACGGGTTGGAAGAGATGGCCAACCTTTGGCTAGATTTTTACTAGAATT1370.10552910908782796No Hit
GGTAGGGATTTCATTCTTTTTTCATGGCTACATAGTATTCCAAGGTGAAAAT1370.10552910908782796No Hit
AGATGTGGGAACATTCTTCTAGCTACATTTGTTGAAAATAAATATTAATCTT1370.10552910908782796No Hit
AGAGACGCGGGCTTCCCTGTGCTGTGCAAGGACCCAGTCCAGGAGGCCTGGG1360.1047588236200336No Hit
TCTGCAGGGGATTGTAACAGGATGCAGACAGGCAGTGAATAATAAGCATAAT1360.1047588236200336No Hit
TAAATAGGGGATCTCAGCTCACCGCAACCTCTGCCTCCCAGATTCAAGCAAT1360.1047588236200336No Hit
TAGCTCGGGCATTAAATATGTGTTAATTGACTGTGTTATTTGTCAGGCTTAC1360.1047588236200336No Hit
GAGAAAGGGAAGCATAACCCTGAGATTCTTACTACTGATGAGAACATTATCT1360.1047588236200336No Hit
GGCGGGGATGACCCACCAATCACATGCCTATCATATAGTAAAACCCAGCCCA1350.10398853815223921No Hit
TAGTGCGGGACCCGAGTGGAGATGCTGGAGATCATCGAGGCTATCTACAAAA1350.10398853815223921No Hit
TCAAAGGGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCC1350.10398853815223921No Hit
GCCACGGGAAACTGAGTTTTTCTTATATTCACAATGGTTTTAATTTTTCTCC1350.10398853815223921No Hit
CATCAGGGAGAAGAGAAAGGTCCTGGAGGCTCCAAGTGGGGCCCTCTCCCTC1350.10398853815223921No Hit
TCGACTAGGGGCTTGAAAATGATTCTCTTAATTACAGTCGTGGAGTTGCTTT1350.10398853815223921No Hit
GCGGTAGGGAGACACAAGGCTGCTAAACCAATTAAGAAAATAATTAATTTCT1340.10321825268444484No Hit
CTCGTTGGGATCTATATTTTGAACTATTGGCCAGTTACTCTGAGTTAAAGAA1340.10321825268444484No Hit
ATATTAGGGACTACTGCACCCACTACCACGCTTGGCTAGTTTTGTATTTTTG1340.10321825268444484No Hit
CAGCCTGGGACCTCAATGGCGAGTTTGTAGGGGGCTGTGACATTCTTCTGCA1340.10321825268444484No Hit
AAACCAGGGAGAAACAGAGGTTTCAGTGAGCCGAGATCATGCCACTGCACTC1330.1024479672166505No Hit
CTATGAGGGGGGAGTGGAGCGGCTGCCAGCCGAGGAGCAGGCGCGGCCGCGG1330.1024479672166505No Hit
CGAGCGGGGGGTGGAAAGTAGAGTATCTTAGCACTAAACCTCAGACTGTTGC1320.10167768174885612No Hit
TCTCAAGGGAGCACATGGGAGACATCCTCTATAAACTTTCCTCCATGAAATT1320.10167768174885612No Hit
TTTAGAGGGCTGGCTGTAAGAACAAAGGGCTGGGCCAGAGAGCCCTTGTAGG1320.10167768174885612No Hit
CTTCTTGGGAGTTCCTGCTCCACCATTTTCCTAGATGATAGCACAGTCAGTC1320.10167768174885612No Hit
TATGTCGGGCTCTAGTTATTTCTAAACAAAATTTTACTTCTTGATGACTACA1310.10090739628106175No Hit
ATAACGGGTACTTGTGCTATCTAAAGCACTACTTTGTAAAGATGACTACCCA1310.10090739628106175No Hit
TTAGCCGGGGTCTTAATGTGTGCTAAGAACCTTGTGGACAGCAAATGAATAA1310.10090739628106175No Hit
GTTACAGGGTAAGAAGTAAAAATAGAGAAGAAATTGAAGAGATTTAAGCATA1310.10090739628106175No Hit
TTCTCGGGGAACTCTTCGTGGGCATGCTCAACAAGCAACAGTCCGAGGACGA1310.10090739628106175No Hit
GCTGCTGGGATATCATGAAACTAAGCTTTTGACAAGATACTTAAAACCTACA1310.10090739628106175No Hit
GGTGGTGGGAAGAAATCCCTTTTCTTTCTGCCATTAAAACCTGCATATCTAT1310.10090739628106175No Hit
CGTACGGGGATTATCGGGTGTCTCAGTCCATCACTTCCATTCTCTGAATCAC1300.10013711081326741No Hit
TGGACCGGGGGGCAGTGAGTGAGGGACCCCTAATGCAGGGACTAGAAGCCTC1300.10013711081326741No Hit
CACACAGGGGTTACACTTACTTTCAGAGTTCTTTTTAGATCTAAAGAAGTCA1300.10013711081326741No Hit
ACCAGGGAGCCGTGGACCATGAAAAATGGCTTCTGCAGGGGCTTAAGAAAGA1300.10013711081326741No Hit
GGCTCCGGGTTATCCCATGAGTGTTTGCATGATGAATTAAGGCCCCACAGAC1300.10013711081326741No Hit
TCATGTGGGATTGTACTTTGCCTTTTCATTCAGTTAGTGGAGTAAGTCATGA1300.10013711081326741No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)