FastQCFastQC Report
Sat 31 Dec 2022
SRR5846906.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5846906.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences129138
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAAGGAGCTATCTCGTATGCCGTCT9540.7387445988012824RNA PCR Primer, Index 38 (95% over 21bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACAAGGAGCTATCTCGTATGC7480.5792253248462884No Hit
GCAACAGGGATTGTGACACTGTGAACATTGGATAATTATAAAAAATAGTGCT1930.1494525236568632No Hit
TGCTATGGGCTTGGGGCCTCCTAAGAAAATGTTTTAATAAGGAAGTCCAAGG1860.1440319658040236No Hit
GATCTCGGGGGCACTCGCAGGCGACCTGCTCCAGTCTCCAAAGCCGATGGCA1840.14248323498892657No Hit
AATGCGTGAAAAGAAAAAACTGAAGAAAAAACTTGTCAGGTCTGAAGAAAAC1780.1378370425436355No Hit
TACGCTGGGATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTA1760.13628831172853845No Hit
GCTGTAGGGGGAGGCCACCGCTGCCCTGCCATCCCGTCTGCCTGCCATGGGA1720.13319085009834442No Hit
TCTCTCGGGATGAGTAATTTATTCTATTGTCCTATAAATATTAATTGAGCAG1700.13164211928324737No Hit
GTGAACGGGAAGTAAAAAATGTGAAGAGAAAAAAATTGCAAACAGAAGAATA1690.13086775387569888No Hit
GAGCTGGGACCTCGGAGAGGGGGAGGCCTCGCGCCGGTGGCTACAGCAAGAG1680.13009338846815036No Hit
CGTTGCGGGGAAACTGAATTAACTTGGGAATATTTGTTGTTAAAAACTTCTT1680.13009338846815036No Hit
GCATATGGGAAACTTGGTGTTCATCAGTAGATAAATAGATGAACAAAATGTT1650.1277702922455048No Hit
CCTGACCTTGTGATCCGCCCGCCTCAGCCCCTCAAAGTACTGGGATTACAGG1600.12389846520776224No Hit
TGTACCGGGAGCTTAAGAGACTTATGGGACACCATCAAGTGACTAATATGTA1580.12234973439266522No Hit
CGTGCCGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1550.12002663817001966No Hit
AGTGCTGGGATCCTCAAATAAGATTAACAGCTGTTTTTCATCAGAAACCATG1520.11770354194737412No Hit
TGATTAGGGTGTCAAAACCAAATGATGATGTATTTTCCTTAAGGCATTGTTT1500.1161548111322771No Hit
GGCTCCGGGTTATCCCATGAGTGTTTGCATGATGAATTAAGGCCCCACAGAC1500.1161548111322771No Hit
TCGACTAGGGGCTTGAAAATGATTCTCTTAATTACAGTCGTGGAGTTGCTTT1500.1161548111322771No Hit
TGTTAGGGGATACATGCTGTTTTTTAATTAAAATATAATCACTGAAGTTTAC1490.11538044572472858No Hit
TGATTAGGGCTGTGTGATAATTTATACGACTCACTTTTGTTTCCGTTCTGTA1490.11538044572472858No Hit
CTCGTTGGGATCTATATTTTGAACTATTGGCCAGTTACTCTGAGTTAAAGAA1490.11538044572472858No Hit
GGGGAAGAGGTTTTATTTACATTTTAGGGTGGGTAAGAAAGCCACCTTGTTA1470.11383171490963157No Hit
AATAACGGGATTGACCATGTGTTCTCATGTCTTTTTGCAGATTTAAAGGTTT1470.11383171490963157No Hit
TCTGCAGGGGATTGTAACAGGATGCAGACAGGCAGTGAATAATAAGCATAAT1460.11305734950208304No Hit
TTTAGAGGGGTTAAGAGAAAAGGTAGTGATTATAATCAGAAAAGCTAGAGTG1460.11305734950208304No Hit
TATCAGGGGATCCATAGAGAAGCCCACCTTCCTGCCTTCCCAGTATTATTTA1440.11150861868698603No Hit
TATACTGGGTAAAAAATACATTGTCCCTTTACACATATGGTTTCTCCTATCA1440.11150861868698603No Hit
TCAAAGGGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCC1430.1107342532794375No Hit
CACTGTGGGAAATAACTTGCCGGGCGGTGGCTCACCCCTGTAATCCCAGCAC1420.10995988787188898No Hit
TGGCATGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1420.10995988787188898No Hit
CTCTGTGGAACAGAATGAAGGAAAAAAATCTCGGTGCAGAAAAAGTATTTGA1410.10918552246434046No Hit
CTACTAGGGATGTATAATGGTGATAAAATATAGCAAGTGAACATGTCGTAAA1400.10841115705679195No Hit
GAGAAAGGGAAGCATAACCCTGAGATTCTTACTACTGATGAGAACATTATCT1400.10841115705679195No Hit
AGATGTGGGAACATTCTTCTAGCTACATTTGTTGAAAATAAATATTAATCTT1400.10841115705679195No Hit
AACCCGGCACATTAGTTTGAAGAGCAAAACTGGAGTACACCTTCCTGAGCCA1390.10763679164924345No Hit
CTGAAAGGGATACTTGGTTTTGGTCATTCTTTTTTCATCCTGCGAACATTTA1390.10763679164924345No Hit
GCCACGGGAAACTGAGTTTTTCTTATATTCACAATGGTTTTAATTTTTCTCC1390.10763679164924345No Hit
CATTCCGGGAGTTGTCTAGTGTAAGCAGAAGCTGATAAATGCTTGTTGCTGA1380.10686242624169492No Hit
GCACATGGGGATCTCAAAGCGGGAGCTGACCCAAGGGGCCTTCGAGGTGCCT1380.10686242624169492No Hit
CATGTTGGGGAGAGCTTTTCTGTGTTTCTCCGGACTTCGAGCCATGGCGGTG1380.10686242624169492No Hit
GCTACTGGGCTCACATCTATAAGCGAGACAGTTTTGCAAATAAATTCATAAA1370.10608806083414642No Hit
GTTACAGGGTAAGAAGTAAAAATAGAGAAGAAATTGAAGAGATTTAAGCATA1370.10608806083414642No Hit
TATTCAGGGATACCTTGAGAAGCAAACTTTGTAGGAAGCCACTCTTCTCCCC1370.10608806083414642No Hit
TTAATTGGGTGATGGCCATTTAAATAGAATTTTATTCTATCTAATTCATTTA1360.10531369542659791No Hit
GAAGCTGGGATCACTTAAAATTGAATTCATCCATTTTTAAAAAACACTTCAT1360.10531369542659791No Hit
CGATCAGGGATACAAAACAACAATACCATAAAATAAAACACTACATAGTTGA1360.10531369542659791No Hit
CATTGTGGGGATAATGAAACCAATCATGGATGCTGTTTTTATTGGGCATGTC1360.10531369542659791No Hit
GTACCTGGGTTGGAAAGGAGAGAGTGAACATAGTGGTGATTGAGGTGGAGGC1360.10531369542659791No Hit
CTTCTTGGGAGTTCCTGCTCCACCATTTTCCTAGATGATAGCACAGTCAGTC1360.10531369542659791No Hit
CAGACTGGGTACAAGTTCTAATGTGCATGTTCCAGCTGCAGTCCCAGTAAGA1350.10453933001904939No Hit
TGATAAGGGACCTCTTGCCTTTTCTGGGCTTGCGTTTCTCTCCTCTAGTGGG1350.10453933001904939No Hit
TGTAGTGGGCCTGTGTGCTGTAAGTGCCATTTCTCAGTATTTTCAAGGCTCT1350.10453933001904939No Hit
CAGCCTGGGACCTCAATGGCGAGTTTGTAGGGGGCTGTGACATTCTTCTGCA1350.10453933001904939No Hit
GCTTGAGGGGGAAGCTGTGCTGTGTGTGTAGACAGGGTGCTGTACTTGTTTG1340.10376496461150088No Hit
CATCAGGGAGAAGAGAAAGGTCCTGGAGGCTCCAAGTGGGGCCCTCTCCCTC1340.10376496461150088No Hit
TACTGTGGGATCATATTTCAAAAACTTCCTATTTCTTGTGTTATGAACCACC1330.10299059920395237No Hit
CGTACGGGGATTATCGGGTGTCTCAGTCCATCACTTCCATTCTCTGAATCAC1330.10299059920395237No Hit
GCAAACGGGGTAAATAAACTGTGGAAAAGATACTTGTTTACAGTATGAGAGC1330.10299059920395237No Hit
GCTAGAGGGGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCA1330.10299059920395237No Hit
TCTAAAGGGATGGACTTCTACAAAATGTTGTATTAATCACTTTTTCTAGTTC1330.10299059920395237No Hit
AAGCACGGGAGCTGAAAATGATTTGGAAGAAACAATTTCATTGCATTGCCAA1330.10299059920395237No Hit
GCACGAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1330.10299059920395237No Hit
TCATGTGGGATTGTACTTTGCCTTTTCATTCAGTTAGTGGAGTAAGTCATGA1330.10299059920395237No Hit
AGATCGGGGATTGTAAGCTAAGCATTAGGACTGATACAAATAATATATGCTC1320.10221623379640385No Hit
GCACTCTGGGAAGATTAGATGATCTCTACTGACCTCTTCTCATAGATTTTGT1320.10221623379640385No Hit
GACGGTGGGCTTCCGATCTCGAGAAGGGGATCTTGCCTGGGAAAGTATTTAG1320.10221623379640385No Hit
CGAATAGGGCACTGGTCAGACATGCTGGCCAACCCCAGGCGACCTATTGCCC1320.10221623379640385No Hit
TATGTCGGGCTCTAGTTATTTCTAAACAAAATTTTACTTCTTGATGACTACA1320.10221623379640385No Hit
TGATTTGGGGAGTCGGAGCCAGCGCGAGCCGCCGCCGCCATCACTGCCGCTG1320.10221623379640385No Hit
TAATGAGGGATCCTCCCACCTGGAGGGTGTGAATGGGGATCAGTTTTTCAAT1310.10144186838885534No Hit
CTCATCGGGTCGCCGGGGGACGCTCTTCCGATCTCATCCGGGGATCTTTGTC1310.10144186838885534No Hit
GGCTAAGGGATCCTTGAGGGCTGGAACCTGAGGATAGAGCGAAAATGGAGAG1310.10144186838885534No Hit
GAAAAAGGGAGGATACTGATTTTAGCTTTTTACTAGGTCCTGACATTCCCGT1310.10144186838885534No Hit
GGTCACGGGGAGACGACCAGGGCCGGCGAGCGCAGTGCAGCGCCGTGCGGTG1310.10144186838885534No Hit
GACGAAGGGGGATAAATCTAACAAAATATGTGTAAGATTTATATGATGGAAA1310.10144186838885534No Hit
TGTATAGGGGGTGGGCCGGGAGCCGTGGGGCGGCATGGAGGGGCTGGTTGTC1310.10144186838885534No Hit
ATAACAGGGATTTCAAGGAAACACACTGGATACTGCCTACTGACAAAACCTA1300.1006675029813068No Hit
TTTACTGGGATGTTAGTGCACATCAGAGAGTTCATACGGGGGAGAAACCCTA1300.1006675029813068No Hit
TTCCCGGGCTTTCTTTTCCCTAATTTGCTTTGGTGGGGTCCTTAAAACATTT1300.1006675029813068No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)