FastQCFastQC Report
Sat 31 Dec 2022
SRR5846896.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5846896.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences142874
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAAGGAGCTATCTCGTATGCCGTCT6450.45144672928594426RNA PCR Primer, Index 38 (95% over 21bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACAAGGAGCTATCTCGTATGC6390.44724722482747037No Hit
GAGTCATCTGACACTGATGATGAAGAATTACGGCATCGAATCCGGCAAAAAC2150.15048224309531477No Hit
CGTGGAGGGATATAGAGGGAGCACCTTTTCTGTGTTCAAAAACTTGTCATGC1490.10428769405210186No Hit
CGTCTGGGGTGGATAAAGAGTCAAGACCCATCAGTGTGCTGTATTCAGGAAA1480.10358777664235619No Hit
AACTAAGGGCCTTTCTACATCCTGCAGGTTTTGCCAGCATGTGCAGCTCACC1460.1021879418228649No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)