FastQCFastQC Report
Sat 31 Dec 2022
SRR5846895.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5846895.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences143382
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACAAGGAGCTATCTCGTATGC6840.4770473281165No Hit
CTTATACACATCTCCGAGCCCACGAGACAAGGAGCTATCTCGTATGCCGTCT6250.43589850887838083RNA PCR Primer, Index 38 (95% over 21bp)
CGTGGAGGGATATAGAGGGAGCACCTTTTCTGTGTTCAAAAACTTGTCATGC1750.12205158248594662No Hit
GAGTCATCTGACACTGATGATGAAGAATTACGGCATCGAATCCGGCAAAAAC1570.10949770543024927No Hit
AACTAAGGGCCTTTCTACATCCTGCAGGTTTTGCCAGCATGTGCAGCTCACC1520.10601051735922222No Hit
TGTACCGGGCAGCTGCACAGGGACCCTACATTTCCCAAAGTCTAGGATCTGG1520.10601051735922222No Hit
CGTCTGGGGTGGATAAAGAGTCAAGACCCATCAGTGTGCTGTATTCAGGAAA1440.10043101644557895No Hit
TCGTTAGGGATGGCTTGCCATAGCTTCTCTAGGCCCAATTTTAGGCCATCAG1440.10043101644557895No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)