FastQCFastQC Report
Sat 31 Dec 2022
SRR5846875.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5846875.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences99839
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAAGGAGCTATCTCGTATGCCGTCT4760.47676759582928513RNA PCR Primer, Index 38 (95% over 21bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACAAGGAGCTATCTCGTATGC4640.464748244673925No Hit
CAGTTGGGGCTGGTAGCTTGACATTTGGTAGTATGAGAATTTTACATGAACA1290.1292080249201214No Hit
TTGTAGGGGAATCTACACTAAGACCAAATGGACCTAATAGATATTTACAAAA1190.11919189895732128No Hit
TACGTTGGGATCTTCCATTCAAAACTGAAGACCTTTATGCAATGCTTCATTA1170.11718867376476128No Hit
GCTTAGGGGAAAGAATAAATGCGTGCATTCCATAGGATGCAAGAAAAGGAGT1130.1131822233796412No Hit
AACGCAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1130.1131822233796412No Hit
TTAACGGGGGTGCAGGGGAAGACCAAAGTTTGCAACTCGAACTGCTTTCGTC1070.10717254780196117No Hit
TTTAAAAAGAGATTAAACCGAAGGTGATTAAAAGACCTTGAAATCCATGACG1070.10717254780196117No Hit
CTTTATGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1010.1011628722242811No Hit
TCTATAGGGGCGGTGCGGCGAGTGAGGCCCCGGTCTTCCTCCTCGTCCTGCC1000.10016125962800108No Hit
CGCAACGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1000.10016125962800108No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)