FastQCFastQC Report
Sat 31 Dec 2022
SRR5846874.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5846874.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences100462
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACAAGGAGCTATCTCGTATGC4470.4449443570703351No Hit
CTTATACACATCTCCGAGCCCACGAGACAAGGAGCTATCTCGTATGCCGTCT4390.4369811471003962RNA PCR Primer, Index 38 (95% over 21bp)
TTAACGGGGGTGCAGGGGAAGACCAAAGTTTGCAACTCGAACTGCTTTCGTC1350.13437916824271864No Hit
CTTTATGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1160.1154665445641138No Hit
CAGTTGGGGCTGGTAGCTTGACATTTGGTAGTATGAGAATTTTACATGAACA1110.11048953833290198No Hit
CGCAACGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1100.10949413708665964No Hit
TTTAAAAAGAGATTAAACCGAAGGTGATTAAAAGACCTTGAAATCCATGACG1090.10849873584041728No Hit
ACATTTGGGGTACAAGTTAGAATGTGGTGCCAGTACAAGTTAGAATGTGGTC1080.10750333459417492No Hit
GCGTAAGGGAAATAAAAGAATAAGTATTGAAATGAAAGAATGACAGAAAGAA1080.10750333459417492No Hit
ACTTGGGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1070.10650793334793256No Hit
CAATAAGGGCACCCGGATTTCCGCAAGGACTTCCATGGCGAGCTGGCGCTGC1070.10650793334793256No Hit
TTGTAGGGGAATCTACACTAAGACCAAATGGACCTAATAGATATTTACAAAA1040.10352172960920547No Hit
AATTCTGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1040.10352172960920547No Hit
ATGTCTGGGGACTATTTTGATTCAAAGGAACTGTCTCAGTTTCTGTGCAAGA1030.1025263283629631No Hit
TACGTTGGGATCTTCCATTCAAAACTGAAGACCTTTATGCAATGCTTCATTA1030.1025263283629631No Hit
GCTTAGGGGAAAGAATAAATGCGTGCATTCCATAGGATGCAAGAAAAGGAGT1020.10153092711672074No Hit
TGAGCCTGGGAGGTCGAGGATGCAGTGAGCTGTGGTCATACCACTGCACTCT1020.10153092711672074No Hit
ACAATTGGGTTGGAAACCAGTGCTAAGAATGCAACGAATGTAGAACAGTCTT1010.10053552587047838No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)