FastQCFastQC Report
Sat 31 Dec 2022
SRR5846873.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5846873.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences100321
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAAGGAGCTATCTCGTATGCCGTCT4580.4565345241773906RNA PCR Primer, Index 38 (95% over 21bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACAAGGAGCTATCTCGTATGC4530.4515505228217422No Hit
TTAACGGGGGTGCAGGGGAAGACCAAAGTTTGCAACTCGAACTGCTTTCGTC1320.13157763578911694No Hit
CTTTATGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1270.12659363443346855No Hit
TTTAAAAAGAGATTAAACCGAAGGTGATTAAAAGACCTTGAAATCCATGACG1260.1255968341623389No Hit
TTTGTGGGGTAGTTTCTGACAAAACAGACTTTGAATCAACAAAGATAAAAAA1150.11463203117991248No Hit
GTAGTAGGGATTCTTTTTAATTTTGCACATGGAACATTTACCAAGATAGACC1140.1136352309087828No Hit
CAGTTGGGGCTGGTAGCTTGACATTTGGTAGTATGAGAATTTTACATGAACA1130.11263843063765314No Hit
CAATAAGGGCACCCGGATTTCCGCAAGGACTTCCATGGCGAGCTGGCGCTGC1120.11164163036652346No Hit
GCTTAGGGGAAAGAATAAATGCGTGCATTCCATAGGATGCAAGAAAAGGAGT1110.11064483009539379No Hit
TACGTTGGGATCTTCCATTCAAAACTGAAGACCTTTATGCAATGCTTCATTA1110.11064483009539379No Hit
ATCCGAGGGATCTTTTAAAAAGAGATTAAACCGAAGGTGATTAAAAGACCTT1100.10964802982426411No Hit
GTTTGAGGGAGTTTAATTTCAGGATCCATCTTTCCATTCATGTTTCTCATTA1070.10665762901087508No Hit
GGTCGCGGGAGAGTCAGTCCAGCGATCAGAAGGCAAGTGGCTTAGAAGACAA1060.10566082873974543No Hit
CAAAAAGGGATATCAAATGAAGTGATAGGGGTCACAATGGGGAAATTGAAGT1050.10466402846861574No Hit
AAAGGCGGGAGAATCGCTTGGACCTGGGAGGCGGAGGTTGCAGTGAGCCTGG1050.10466402846861574No Hit
ACTTGGGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1040.10366722819748608No Hit
TCTATAGGGGCGGTGCGGCGAGTGAGGCCCCGGTCTTCCTCCTCGTCCTGCC1040.10366722819748608No Hit
GCACTAGGGCAAAGCGCTGAGGCTGTGCACCTATTCTGTAGTTGCAGCTGAT1020.10167362765522672No Hit
ATGTCTGGGGACTATTTTGATTCAAAGGAACTGTCTCAGTTTCTGTGCAAGA1010.10067682738409704No Hit
TTGTAGGGGAATCTACACTAAGACCAAATGGACCTAATAGATATTTACAAAA1010.10067682738409704No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)