FastQCFastQC Report
Sat 31 Dec 2022
SRR5846869.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5846869.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19460
Sequences flagged as poor quality0
Sequence length52
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAAGGAGCTATCTCGTATGCCGTCT4442.2816032887975335RNA PCR Primer, Index 38 (95% over 21bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACAAGGAGCTATCTCGTATGC4402.2610483042137717No Hit
CAAGCTGGGAGAAAAACTGAGACTGTACAAAGAGGAGAGAAAGAAGAAATCA1540.7913669064748201No Hit
GCTCAGGGGAGGGACTGGAGGGAGTCGGAGAAAAAGCTGACCTAATGAAACT1060.5447070914696814No Hit
ACCTAAGAACAGCTAAAAGAGCACACCCGTCTATGTAGCAAAATAGTGGGAA890.4573484069886948No Hit
ACACCTGGGGAGAGCTACGCAGCCTTCGGTGCAGTCGTCACTCGTGTCTCGC840.4316546762589928No Hit
TGCTTAGGGATAAGTCAAAAGAAGACATGTCTACAGAGAAAGTTTGAGTGAT810.4162384378211716No Hit
TGGCATGGGAAATATGCGTTTATTTAAAAGGAGAACAAAAGCTTGACTCTGA800.41109969167523125No Hit
CTGCAGGGGAGGAGCTCCGCGCGGCTGCAGCGCGGGCGGGAGCGGGGACGCG800.41109969167523125No Hit
TAGTCGGGGACGACGCTCTTCCGATCTTGTACTGGGGGTGGCGGATCATGGT760.39054470709146966No Hit
AAGTTTGGGGGAGCAGGAACAGGTTGAACAGTCTACCCTCCCTTAGCAGGGA750.3854059609455293No Hit
CTTGCTTAGGGCATGGCTGAGTGCCTTCACGAGGTCGGATTGCGTGAGGATG730.3751284686536485No Hit
TGTAATGGGCAGGCCTGACGGATGACACCCACACAGACCCTGTGTCCCAGGT710.3648509763617677No Hit
TTACCAGGGCTGGGTTTGATTTGAGTTCACCTGTGGGCAGTGGGCAGTGGGC710.3648509763617677No Hit
CTACGTGGGCACTGATCTGTTTCAACATTTCTTTGTTGCTATGCTGTAGGTG680.3494347379239465No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGGAGCT680.3494347379239465No Hit
TTAGAAGGGGCTTCATTACTGGACTAGAGGGTTGAGCCACCCACTTGCCATT670.3442959917780062No Hit
ATCTCTGGGGGGGCCTCTCTCCGTCGCCATGGAAACGAAAGCGGCCAAGTAG670.3442959917780062No Hit
CTGTTAGGGCCATGGGTGCGCTTGATGGTTTAGTGGATCGACTCAACTCAGA630.3237410071942446No Hit
ATCCCTGGGATGACCGGCTGCGGCCCCTGTCCTACCAGAACACCCACCTCGT630.3237410071942446No Hit
ACTCAGGGGGTATCGACGTATTCATCTCTCTTCGGACCCTTACCCTCTTTAC610.31346351490236385No Hit
ACAATTGGGATTATCTGTCCAAGATTGAAGAACTCCAGCTGATTGTGAATGA610.31346351490236385No Hit
TCGTACGGGAACCACTGGTGTAAAACCTTAGTTATATATGATCTGCATTTTC610.31346351490236385No Hit
TAGCGGGGGATAGTGGCAGCTGACCAATCCCCCATCCCAAATTGACCATTTA600.3083247687564234No Hit
GTAAAGGGGATTTGTGCCAATTTCTGGTTGGAATGGTGACAACATGCTGGAG600.3083247687564234No Hit
AGATATGGGTGCCCTGTGTGACAGGCACACCAAATTTTTTGAAGAGCCGGAA590.30318602261048305No Hit
GCTACTGGGGGGTTCGAACACGGCACCCGCACTGCGCGTCATGGTGCAGGCC580.29804727646454265No Hit
TGCGTAGGGATACCAAGGCCACCACACACCACCTGTCCAAAAAGGCCTTCGA580.29804727646454265No Hit
TTGCAAGGTACCTCTGAGTTGTGTTCCACGGAAAATGCTATCCAGCAGATCA560.28776978417266186No Hit
TGGTCAGGGCGTGCCCCAGCCTGGACCCCTGGGGCTCAGATAGAGGTGCTGA560.28776978417266186No Hit
GGCATTGGGAAAGAACCAAAACCATCCCTGAAATGCCTTGCTAATACAACTA560.28776978417266186No Hit
TAGACAGGGATGACCCACCAATCACATGCCTATCATATAGTAAAACCCAGCC550.28263103802672146No Hit
GCAACTGGGAGAGGAGAGGAGCGGCCGGAGCGAGACACTTCGCCGAGGCACA540.2774922918807811No Hit
ATTTTAGGGAAGCTTGAAGGCATTGGAATAGTAAAATATTATGAAACATTAC540.2774922918807811No Hit
TAGTAGGGGAGTCCGAGTGGAGAGAGCGAGCTGAGTGGTTGTGTGGTCGCGT540.2774922918807811No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGGAGCTA540.2774922918807811No Hit
TGTAGGTGGGGAAGAACCCAGCCAAAGTGAAAGTCAGCAGGGGAATAAGAAC530.2723535457348407No Hit
ATAGAGGGGGAGCCTACACTTAGCAACATCATCGAGCAGCGCAGCCTGAAGT520.26721479958890026No Hit
AAGACTGGGATGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCT490.2517985611510791No Hit
TTTTGTGGGGATAGGATACATCTTGGACATGGAATTGTTAAGCCACCTCTGA490.2517985611510791No Hit
TCTGCAGGGATTATGATAATCATATTTACCAAATGCCCCTCATTTACATAAA480.24665981500513876No Hit
AGGCCAGGGGTCGGCGGCCGCGCGCAGCACGCTCAAGGCCGGGATGGCGGCG480.24665981500513876No Hit
GAAGCAGGGAACTTCTTTTCAGTTAGCATTATACTTGATATATCTATTCCAT470.24152106885919836No Hit
CTCGCGGGGAGGGTTGAATCTGAATTGGCTTCTCAGGCTCAAGGTAACAGTG460.236382322713258No Hit
GACCATGGGGGGGGGCGCGCGCGCGGGCCTGGGGAGCTCGCGGGGAGAGCCC460.236382322713258No Hit
CTCCCGGGGATCCTGCGGCTGGAGGGCGTGGTCACCAAGAGCCGACCCCTCA450.2312435765673176No Hit
GGATATGGGATGAGCAAATATGTCAAGGGAGTCAATTTCTGACCAATCAAGT450.2312435765673176No Hit
TTAATAGGGTATAATTTCAGCCCCCAGCCAACCCTTTTTATCCAGAATGTCC450.2312435765673176No Hit
CGTAAAGGGGGATGACGAATTTGAATGCTATCTAGTGGGTCAGGGGAAGTCA440.22610483042137716No Hit
ATCTTGGGGCCGATCAAACTGTGGGAGTGTTTGTTTTTGCACACCATTTTGT430.2209660842754368No Hit
GTTAGAGGGGCAGATAATTTTTAGCTTAGGGCCTGGTGGCTAGGTCGGTTCT410.21068859198355602No Hit
TGCCGCGGGATTGAAGAAAGTTTTCTTTGCCTTGCTTTGTTTTGCCTTACCC410.21068859198355602No Hit
AGTAAAGGGTTGGTTGGACACAGCGCTTAGTGCACGCTGTCATCATGGACAT400.20554984583761562No Hit
CTTGTAGGGTATTACTCCATTACTATTAACTATCGGAAAAATGAACTGGAAC400.20554984583761562No Hit
CACACCGGGATTGTGGGAGTACAGCTCTTTCCTTTCCGTCTGGCGGCAGCCA400.20554984583761562No Hit
AAGGCAGGACTAATACTGAGTGATTGCAGAGTGCTTTATAAATATCACCTTA390.20041109969167523No Hit
TTGCCAGGGCATTTTAAAATCTTTTGCCTATACTTTGATATATACACCTTGG390.20041109969167523No Hit
GCTAAGGGGAATGGGCTCAAATTCTCTACCTCTTCAGGGCTAATACTTTTAA370.19013360739979446No Hit
AAACAAGGCATTCCAGTGGAAGACGAATGTAACCTGCGTGGTGATGTGACAA370.19013360739979446No Hit
TTGCATGGGTAAAAATAGAATTGCCTTAAAGGATTTGAAGTAAGAACATAAT370.19013360739979446No Hit
AAAGCTGGGCCTAGCAAAAAGTCTGAAATTTATCTTTCTTACAAACTGTTCC370.19013360739979446No Hit
ACTCTCGGGAGCTGACCCCCTCCCCCCAATCCCTCAAACCTTGAGGAGAAGT370.19013360739979446No Hit
TGCACTGGGATAAAAATGTTGCATTAGTTGCAACATACATATAAAAATGCTT360.18499486125385406No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACAAGGAGCTATCCTGTCTCT340.17471736896197326No Hit
CTTATACACATCTCCGAGCCCACGAGACAAGGAGCTACTGTCTCTTATACAC340.17471736896197326No Hit
AGCAAAGGGAGTAGCCCTCTGGGCCTGGGATATTGTGGTTGATAACTGTGCC330.1695786228160329No Hit
AAAGCAGGGAGCATATGCATTTCCCCACCGCGTTGTGTCTGCAGCTTCTTTG330.1695786228160329No Hit
CATAGCGGGACATCAGGCAGAGGAGAGTGCAGCCTCACAGTGACTTTCTCAG320.1644398766700925No Hit
CTAGCGGGGGTCGTACGTGCTGTGCTACGACCCGCGCACCCGCGGCGCGCTC320.1644398766700925No Hit
TAGGTTGGGAGCCGGGGACATCGTCGGGCTTCCCGGGACCGAAGTGCGAAAT320.1644398766700925No Hit
CTTATACACATCTCCGAGCCCACGAGACAAGGAGCTATCCTGTCTCTTATAC320.1644398766700925No Hit
TGCTGAGGGCAAGCCAAATACCAGATCCGCTACCGAGTGGAGGACAGTGTGG310.15930113052415212No Hit
TTCTTAGGGATTTGTAAAATCAACTTTTCAAATAATGTAAATGTAATATACT300.1541623843782117No Hit
TCAACAGGGATTCCTTCAAATATGTTTTCTGTGTTGTATACTTTTTCTCCTC300.1541623843782117No Hit
TCGTAAGGGGGAGCTGGGGTGGCCCGGGGAGGCGCGGGCGGCGGCCCCAGCG300.1541623843782117No Hit
CATCTTGGGAGGAACAGACAAACTCAACCAGAACTCCCAGCTGAGCCACAGT300.1541623843782117No Hit
TTCATTGGGGCGGCTGCACCAGCTGGCCGCGGGCGCCAGGCTGGAGCCGGCC300.1541623843782117No Hit
TCTTGAGGGCAGCAGAGTACAGAAGGGTGAAGAAGTGAGCAGAGGCTTATGA300.1541623843782117No Hit
TGACAGGGGTCCGACTCTTCCTGGGAGAAGGGCAATGCTTTTAGATTAAAAT290.14902363823227133No Hit
GCTTGTGGGACTGCCTAATTTTCTCACCCACATTCGCTGAAGCGATGGAGAG290.14902363823227133No Hit
ATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGGAGCTATCTCGT290.14902363823227133No Hit
AAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGGAGCTATCTC290.14902363823227133No Hit
GTATACGGGAAGCTAGGAGCCGCGCGAGTCGTAGTGTCGCTGTTTGCGGGTC280.14388489208633093No Hit
CCCAACCGGGGAATAACAACTAGAAAAGGCAGTATACTTCACTGATGCTTGT280.14388489208633093No Hit
GCTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGGAGCTATCTCGTAT280.14388489208633093No Hit
ATCTGAAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGGAGCTAT270.13874614594039056No Hit
GTACATGGGACAGGACAACTGGCAAGTTTGCTGGGAAGTCAAATAATACATT270.13874614594039056No Hit
CAGAATGGGACTGGGAGTGCTACCAGAACTACCACAGGATGAAAGTGGTGAG270.13874614594039056No Hit
GCTAGTGGGTAATCACAGCAGTCCTACTTCTCCTATCTCTCCCAGTCCTAGC270.13874614594039056No Hit
AGTCCAGGGAGGCCTGCAAGGCCCAAGTGGACGCTCGGACAAGGGCAGGAAG250.12846865364850976No Hit
TTACAGGGGGGTTTCTAAGAGCCTTTCTTGCAGCTAATTCCATAAAGTGTCT250.12846865364850976No Hit
TATTCGGGGAGACCATGTGTTCACGGGATGCCCGCACAAAACAGCTGAGGCA250.12846865364850976No Hit
TATCGAGGGGAGGGTCTGGATGCCCACTTTCCATGCAGCAGCATTTCATTGC250.12846865364850976No Hit
TTGGCTGGGCGAGTGTAGCCCTGTTTCACTCGGATCAGGTTGGCACGGCCGC250.12846865364850976No Hit
CTTTGGGGGGGTGGCCTGGGCGGCGTTGGTCCGGTGCGTCCTGTTCTACAGC250.12846865364850976No Hit
CAGCAGGGGTGCTCAGACACATTTAGGTTCAGGGTGGACCTTTATGTCTTGT240.12332990750256938No Hit
TTGCAGGGGGCCAAAAGACATCTTACACAATCCACCTTTAACTTTTCAAGTT230.118191161356629No Hit
ACTGCCGGGTCTCGCACTTCAACTGGCCGGTGCAGGGCGCCAACTACCACGT220.11305241521068858No Hit
GTTGCCGGGTTTTTTTTTTCTTGATGTAAACGTGTACAGCTGTTTTATTAGT220.11305241521068858No Hit
TGACTAGGGACTGTAAGACGTATGTAACATGATGTTAACTTTGTGTGGTCTA220.11305241521068858No Hit
GGTAGCGGGATGTGGAAAGCTTGTCTTAGAGGGCTTTTCTTTGTATGTGTAG220.11305241521068858No Hit
CAATAAGGGAAGTGAAGTGATGGCGAATCCTCCCCAAGTCGAGACCGTGACC220.11305241521068858No Hit
GTATTTGGGATCATAGGAGGCTTCATTCACTGATTTCCCCTATTCTCAGGCT210.1079136690647482No Hit
TCATATGGGGGACTAACCCCTATACCTTCTGCATAATGAATTAACTAGAAAT210.1079136690647482No Hit
ATAAAAGGGAGGGCCCGCCACCCGAGGGTCGCGCAGCCCACTGGGCCCGATG210.1079136690647482No Hit
GCTGCTGAGTTGAGTTACCAGACATCCTCCATGTGAGAAGCAGCGAACATTG210.1079136690647482No Hit
AGCGCCGGGAGCCTTCCGCCCTGGTATTTGGGGGGATGACCAGCCCAGGCGT210.1079136690647482No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACAAGGAGCTAACTCGTATGC200.10277492291880781No Hit
CGTGAGGGGGAGCTGTTTAGTAATAACCCAGTTTTCTTGAGGTCTGTTTACT200.10277492291880781No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)