FastQCFastQC Report
Sat 31 Dec 2022
SRR5846868.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5846868.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19415
Sequences flagged as poor quality0
Sequence length52
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAAGGAGCTATCTCGTATGCCGTCT4582.3590007725985065RNA PCR Primer, Index 38 (95% over 21bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACAAGGAGCTATCTCGTATGC4452.2920422353850114No Hit
CAAGCTGGGAGAAAAACTGAGACTGTACAAAGAGGAGAGAAAGAAGAAATCA1430.7365439093484419No Hit
GCTCAGGGGAGGGACTGGAGGGAGTCGGAGAAAAAGCTGACCTAATGAAACT990.509915014164306No Hit
CTGCAGGGGAGGAGCTCCGCGCGGCTGCAGCGCGGGCGGGAGCGGGGACGCG940.48416173062065415No Hit
ATCTCTGGGGGGGCCTCTCTCCGTCGCCATGGAAACGAAAGCGGCCAAGTAG930.4790110739119237No Hit
CTGTTAGGGCCATGGGTGCGCTTGATGGTTTAGTGGATCGACTCAACTCAGA900.46355910378573273No Hit
TGCTTAGGGATAAGTCAAAAGAAGACATGTCTACAGAGAAAGTTTGAGTGAT890.45840844707700235No Hit
TAGTAGGGGAGTCCGAGTGGAGAGAGCGAGCTGAGTGGTTGTGTGGTCGCGT790.4069018799896987No Hit
ACACCTGGGGAGAGCTACGCAGCCTTCGGTGCAGTCGTCACTCGTGTCTCGC760.3914499098635076No Hit
ACCTAAGAACAGCTAAAAGAGCACACCCGTCTATGTAGCAAAATAGTGGGAA750.38629925315477726No Hit
AGGCCAGGGGTCGGCGGCCGCGCGCAGCACGCTCAAGGCCGGGATGGCGGCG750.38629925315477726No Hit
AAGTTTGGGGGAGCAGGAACAGGTTGAACAGTCTACCCTCCCTTAGCAGGGA730.37599793973731654No Hit
ATAGAGGGGGAGCCTACACTTAGCAACATCATCGAGCAGCGCAGCCTGAAGT710.3656966263198558No Hit
TGTAGGTGGGGAAGAACCCAGCCAAAGTGAAAGTCAGCAGGGGAATAAGAAC700.36054596961112545No Hit
TTAGAAGGGGCTTCATTACTGGACTAGAGGGTTGAGCCACCCACTTGCCATT690.35539531290239507No Hit
ACAATTGGGATTATCTGTCCAAGATTGAAGAACTCCAGCTGATTGTGAATGA690.35539531290239507No Hit
TGTAATGGGCAGGCCTGACGGATGACACCCACACAGACCCTGTGTCCCAGGT680.3502446561936647No Hit
AGATATGGGTGCCCTGTGTGACAGGCACACCAAATTTTTTGAAGAGCCGGAA640.32964202935874326No Hit
ATCCCTGGGATGACCGGCTGCGGCCCCTGTCCTACCAGAACACCCACCTCGT630.3244913726500129No Hit
CTTGCTTAGGGCATGGCTGAGTGCCTTCACGAGGTCGGATTGCGTGAGGATG620.3193407159412825No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGGAGCT620.3193407159412825No Hit
CTACGTGGGCACTGATCTGTTTCAACATTTCTTTGTTGCTATGCTGTAGGTG610.31419005923255217No Hit
GGATATGGGATGAGCAAATATGTCAAGGGAGTCAATTTCTGACCAATCAAGT610.31419005923255217No Hit
TGGTCAGGGCGTGCCCCAGCCTGGACCCCTGGGGCTCAGATAGAGGTGCTGA600.3090394025238218No Hit
GGCATTGGGAAAGAACCAAAACCATCCCTGAAATGCCTTGCTAATACAACTA600.3090394025238218No Hit
TGGCATGGGAAATATGCGTTTATTTAAAAGGAGAACAAAAGCTTGACTCTGA600.3090394025238218No Hit
TTAATAGGGTATAATTTCAGCCCCCAGCCAACCCTTTTTATCCAGAATGTCC600.3090394025238218No Hit
TTACCAGGGCTGGGTTTGATTTGAGTTCACCTGTGGGCAGTGGGCAGTGGGC590.30388874581509145No Hit
GTAAAGGGGATTTGTGCCAATTTCTGGTTGGAATGGTGACAACATGCTGGAG590.30388874581509145No Hit
ACTCAGGGGGTATCGACGTATTCATCTCTCTTCGGACCCTTACCCTCTTTAC580.29873808910636107No Hit
TTGCAAGGTACCTCTGAGTTGTGTTCCACGGAAAATGCTATCCAGCAGATCA580.29873808910636107No Hit
TAGTCGGGGACGACGCTCTTCCGATCTTGTACTGGGGGTGGCGGATCATGGT580.29873808910636107No Hit
ATTTTAGGGAAGCTTGAAGGCATTGGAATAGTAAAATATTATGAAACATTAC570.2935874323976307No Hit
TCTGCAGGGATTATGATAATCATATTTACCAAATGCCCCTCATTTACATAAA560.28843677568890036No Hit
GACCATGGGGGGGGGCGCGCGCGCGGGCCTGGGGAGCTCGCGGGGAGAGCCC550.28328611898017No Hit
TGCGTAGGGATACCAAGGCCACCACACACCACCTGTCCAAAAAGGCCTTCGA540.2781354622714396No Hit
CACACCGGGATTGTGGGAGTACAGCTCTTTCCTTTCCGTCTGGCGGCAGCCA540.2781354622714396No Hit
TCGTACGGGAACCACTGGTGTAAAACCTTAGTTATATATGATCTGCATTTTC530.2729848055627092No Hit
TAGCGGGGGATAGTGGCAGCTGACCAATCCCCCATCCCAAATTGACCATTTA500.25753283543651817No Hit
ATCTTGGGGCCGATCAAACTGTGGGAGTGTTTGTTTTTGCACACCATTTTGT480.24723152201905743No Hit
CATAGCGGGACATCAGGCAGAGGAGAGTGCAGCCTCACAGTGACTTTCTCAG460.23693020860159672No Hit
AAGACTGGGATGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCT460.23693020860159672No Hit
TTCTTAGGGATTTGTAAAATCAACTTTTCAAATAATGTAAATGTAATATACT450.23177955189286636No Hit
GCAACTGGGAGAGGAGAGGAGCGGCCGGAGCGAGACACTTCGCCGAGGCACA450.23177955189286636No Hit
CTCGCGGGGAGGGTTGAATCTGAATTGGCTTCTCAGGCTCAAGGTAACAGTG450.23177955189286636No Hit
GAAGCAGGGAACTTCTTTTCAGTTAGCATTATACTTGATATATCTATTCCAT450.23177955189286636No Hit
TAGACAGGGATGACCCACCAATCACATGCCTATCATATAGTAAAACCCAGCC440.22662889518413595No Hit
GCTACTGGGGGGTTCGAACACGGCACCCGCACTGCGCGTCATGGTGCAGGCC440.22662889518413595No Hit
CGTAAAGGGGGATGACGAATTTGAATGCTATCTAGTGGGTCAGGGGAAGTCA440.22662889518413595No Hit
AAAGCTGGGCCTAGCAAAAAGTCTGAAATTTATCTTTCTTACAAACTGTTCC440.22662889518413595No Hit
GCTAAGGGGAATGGGCTCAAATTCTCTACCTCTTCAGGGCTAATACTTTTAA430.2214782384754056No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGGAGCTA430.2214782384754056No Hit
TTTTGTGGGGATAGGATACATCTTGGACATGGAATTGTTAAGCCACCTCTGA410.2111769250579449No Hit
AAACAAGGCATTCCAGTGGAAGACGAATGTAACCTGCGTGGTGATGTGACAA400.20602626834921456No Hit
TCGTAAGGGGGAGCTGGGGTGGCCCGGGGAGGCGCGGGCGGCGGCCCCAGCG380.1957249549317538No Hit
AAAGCAGGGAGCATATGCATTTCCCCACCGCGTTGTGTCTGCAGCTTCTTTG380.1957249549317538No Hit
TATCGAGGGGAGGGTCTGGATGCCCACTTTCCATGCAGCAGCATTTCATTGC370.19057429822302344No Hit
TGCCGCGGGATTGAAGAAAGTTTTCTTTGCCTTGCTTTGTTTTGCCTTACCC370.19057429822302344No Hit
CTTATACACATCTCCGAGCCCACGAGACAAGGAGCTACTGTCTCTTATACAC360.18542364151429305No Hit
TTGCATGGGTAAAAATAGAATTGCCTTAAAGGATTTGAAGTAAGAACATAAT360.18542364151429305No Hit
TTGCCAGGGCATTTTAAAATCTTTTGCCTATACTTTGATATATACACCTTGG350.18027298480556272No Hit
CCCAACCGGGGAATAACAACTAGAAAAGGCAGTATACTTCACTGATGCTTGT350.18027298480556272No Hit
TGACAGGGGTCCGACTCTTCCTGGGAGAAGGGCAATGCTTTTAGATTAAAAT340.17512232809683234No Hit
CTCCCGGGGATCCTGCGGCTGGAGGGCGTGGTCACCAAGAGCCGACCCCTCA340.17512232809683234No Hit
GTTAGAGGGGCAGATAATTTTTAGCTTAGGGCCTGGTGGCTAGGTCGGTTCT340.17512232809683234No Hit
CTAGCGGGGGTCGTACGTGCTGTGCTACGACCCGCGCACCCGCGGCGCGCTC340.17512232809683234No Hit
ATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGGAGCTATCTCGT330.16997167138810199No Hit
CATCTTGGGAGGAACAGACAAACTCAACCAGAACTCCCAGCTGAGCCACAGT330.16997167138810199No Hit
TTCATTGGGGCGGCTGCACCAGCTGGCCGCGGGCGCCAGGCTGGAGCCGGCC330.16997167138810199No Hit
AGTAAAGGGTTGGTTGGACACAGCGCTTAGTGCACGCTGTCATCATGGACAT320.16482101467937163No Hit
AAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGGAGCTATCTC320.16482101467937163No Hit
TTGGCTGGGCGAGTGTAGCCCTGTTTCACTCGGATCAGGTTGGCACGGCCGC320.16482101467937163No Hit
CTTTGGGGGGGTGGCCTGGGCGGCGTTGGTCCGGTGCGTCCTGTTCTACAGC320.16482101467937163No Hit
CTTATACACATCTCCGAGCCCACGAGACAAGGAGCTATCCTGTCTCTTATAC320.16482101467937163No Hit
AAGGCAGGACTAATACTGAGTGATTGCAGAGTGCTTTATAAATATCACCTTA310.15967035797064125No Hit
CTTGTAGGGTATTACTCCATTACTATTAACTATCGGAAAAATGAACTGGAAC310.15967035797064125No Hit
TTGCAGGGGGCCAAAAGACATCTTACACAATCCACCTTTAACTTTTCAAGTT310.15967035797064125No Hit
TATTCGGGGAGACCATGTGTTCACGGGATGCCCGCACAAAACAGCTGAGGCA280.14421838784445018No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACAAGGAGCTAACTCGTATGC280.14421838784445018No Hit
TTACAGGGGGGTTTCTAAGAGCCTTTCTTGCAGCTAATTCCATAAAGTGTCT270.1390677311357198No Hit
CGTGAGGGGGAGCTGTTTAGTAATAACCCAGTTTTCTTGAGGTCTGTTTACT270.1390677311357198No Hit
TGCTGAGGGCAAGCCAAATACCAGATCCGCTACCGAGTGGAGGACAGTGTGG270.1390677311357198No Hit
GTATACGGGAAGCTAGGAGCCGCGCGAGTCGTAGTGTCGCTGTTTGCGGGTC260.13391707442698944No Hit
TAGGTTGGGAGCCGGGGACATCGTCGGGCTTCCCGGGACCGAAGTGCGAAAT260.13391707442698944No Hit
AGTCCAGGGAGGCCTGCAAGGCCCAAGTGGACGCTCGGACAAGGGCAGGAAG250.12876641771825909No Hit
TGCACTGGGATAAAAATGTTGCATTAGTTGCAACATACATATAAAAATGCTT250.12876641771825909No Hit
CAGAATGGGACTGGGAGTGCTACCAGAACTACCACAGGATGAAAGTGGTGAG250.12876641771825909No Hit
TCTTGAGGGCAGCAGAGTACAGAAGGGTGAAGAAGTGAGCAGAGGCTTATGA250.12876641771825909No Hit
TCAACAGGGATTCCTTCAAATATGTTTTCTGTGTTGTATACTTTTTCTCCTC240.12361576100952872No Hit
ATAAAAGGGAGGGCCCGCCACCCGAGGGTCGCGCAGCCCACTGGGCCCGATG240.12361576100952872No Hit
ACTCTCGGGAGCTGACCCCCTCCCCCCAATCCCTCAAACCTTGAGGAGAAGT240.12361576100952872No Hit
AGCAAAGGGAGTAGCCCTCTGGGCCTGGGATATTGTGGTTGATAACTGTGCC230.11846510430079836No Hit
ATCTGAAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGGAGCTAT220.11331444759206798No Hit
ACTGCCGGGTCTCGCACTTCAACTGGCCGGTGCAGGGCGCCAACTACCACGT220.11331444759206798No Hit
AGCGCCGGGAGCCTTCCGCCCTGGTATTTGGGGGGATGACCAGCCCAGGCGT220.11331444759206798No Hit
GCTAGTGGGTAATCACAGCAGTCCTACTTCTCCTATCTCTCCCAGTCCTAGC220.11331444759206798No Hit
TGACTAGGGACTGTAAGACGTATGTAACATGATGTTAACTTTGTGTGGTCTA220.11331444759206798No Hit
GGTAGCGGGATGTGGAAAGCTTGTCTTAGAGGGCTTTTCTTTGTATGTGTAG220.11331444759206798No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACAAGGAGCTATCCTGTCTCT210.10816379088333762No Hit
GCTGCTGAGTTGAGTTACCAGACATCCTCCATGTGAGAAGCAGCGAACATTG210.10816379088333762No Hit
TTCTTGCGGGCGAACTACTATACTCAATTGATCCAATAACTTGACCAACGGA200.10301313417460728No Hit
CCATCCGGGTACATTTTCAAGTTTATTAGGTTAAGAAAAACAGGGCAGCCTT200.10301313417460728No Hit
GTACATGGGACAGGACAACTGGCAAGTTTGCTGGGAAGTCAAATAATACATT200.10301313417460728No Hit
CAGCAGGGGTGCTCAGACACATTTAGGTTCAGGGTGGACCTTTATGTCTTGT200.10301313417460728No Hit
CATCCAGGGAGCCTGCTTCCGGATGGCCCCCTTATATAATCTGCCAAGGCAT200.10301313417460728No Hit
GCTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGGAGCTATCTCGTAT200.10301313417460728No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)