FastQCFastQC Report
Sat 31 Dec 2022
SRR5846867.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5846867.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18513
Sequences flagged as poor quality0
Sequence length52
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAAGGAGCTATCTCGTATGCCGTCT4382.3659050397018313RNA PCR Primer, Index 38 (95% over 21bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACAAGGAGCTATCTCGTATGC4302.322692162264355No Hit
CAAGCTGGGAGAAAAACTGAGACTGTACAAAGAGGAGAGAAAGAAGAAATCA1300.702209258358991No Hit
TGCTTAGGGATAAGTCAAAAGAAGACATGTCTACAGAGAAAGTTTGAGTGAT1000.5401609679684547No Hit
GCTCAGGGGAGGGACTGGAGGGAGTCGGAGAAAAAGCTGACCTAATGAAACT940.5077513098903473No Hit
CTGTTAGGGCCATGGGTGCGCTTGATGGTTTAGTGGATCGACTCAACTCAGA890.4807432614919246No Hit
CTGCAGGGGAGGAGCTCCGCGCGGCTGCAGCGCGGGCGGGAGCGGGGACGCG840.45373521309350184No Hit
TAGTAGGGGAGTCCGAGTGGAGAGAGCGAGCTGAGTGGTTGTGTGGTCGCGT790.4267271646950791No Hit
CTTGCTTAGGGCATGGCTGAGTGCCTTCACGAGGTCGGATTGCGTGAGGATG780.4213255550153946No Hit
ACACCTGGGGAGAGCTACGCAGCCTTCGGTGCAGTCGTCACTCGTGTCTCGC780.4213255550153946No Hit
ACCTAAGAACAGCTAAAAGAGCACACCCGTCTATGTAGCAAAATAGTGGGAA760.4105223356560255No Hit
ATAGAGGGGGAGCCTACACTTAGCAACATCATCGAGCAGCGCAGCCTGAAGT720.3889158969372873No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGGAGCT710.3835142872576028No Hit
ATCTCTGGGGGGGCCTCTCTCCGTCGCCATGGAAACGAAAGCGGCCAAGTAG680.3673094582185491No Hit
AAGTTTGGGGGAGCAGGAACAGGTTGAACAGTCTACCCTCCCTTAGCAGGGA680.3673094582185491No Hit
ACAATTGGGATTATCTGTCCAAGATTGAAGAACTCCAGCTGATTGTGAATGA670.3619078485388646No Hit
TTAGAAGGGGCTTCATTACTGGACTAGAGGGTTGAGCCACCCACTTGCCATT650.3511046291794955No Hit
ACTCAGGGGGTATCGACGTATTCATCTCTCTTCGGACCCTTACCCTCTTTAC620.33489980014044185No Hit
TTACCAGGGCTGGGTTTGATTTGAGTTCACCTGTGGGCAGTGGGCAGTGGGC620.33489980014044185No Hit
TGTAGGTGGGGAAGAACCCAGCCAAAGTGAAAGTCAGCAGGGGAATAAGAAC620.33489980014044185No Hit
TCGTACGGGAACCACTGGTGTAAAACCTTAGTTATATATGATCTGCATTTTC620.33489980014044185No Hit
TAGACAGGGATGACCCACCAATCACATGCCTATCATATAGTAAAACCCAGCC570.30789175174201916No Hit
CTCCCGGGGATCCTGCGGCTGGAGGGCGTGGTCACCAAGAGCCGACCCCTCA560.30249014206233454No Hit
CTACGTGGGCACTGATCTGTTTCAACATTTCTTTGTTGCTATGCTGTAGGTG560.30249014206233454No Hit
AAGACTGGGATGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCT550.29708853238265004No Hit
TAGTCGGGGACGACGCTCTTCCGATCTTGTACTGGGGGTGGCGGATCATGGT550.29708853238265004No Hit
TGGCATGGGAAATATGCGTTTATTTAAAAGGAGAACAAAAGCTTGACTCTGA550.29708853238265004No Hit
TTAATAGGGTATAATTTCAGCCCCCAGCCAACCCTTTTTATCCAGAATGTCC550.29708853238265004No Hit
TTGCAAGGTACCTCTGAGTTGTGTTCCACGGAAAATGCTATCCAGCAGATCA540.2916869227029655No Hit
AGGCCAGGGGTCGGCGGCCGCGCGCAGCACGCTCAAGGCCGGGATGGCGGCG540.2916869227029655No Hit
CACACCGGGATTGTGGGAGTACAGCTCTTTCCTTTCCGTCTGGCGGCAGCCA540.2916869227029655No Hit
CTCGCGGGGAGGGTTGAATCTGAATTGGCTTCTCAGGCTCAAGGTAACAGTG530.2862853130232809No Hit
TAGCGGGGGATAGTGGCAGCTGACCAATCCCCCATCCCAAATTGACCATTTA520.28088370334359636No Hit
GCAACTGGGAGAGGAGAGGAGCGGCCGGAGCGAGACACTTCGCCGAGGCACA520.28088370334359636No Hit
ATCCCTGGGATGACCGGCTGCGGCCCCTGTCCTACCAGAACACCCACCTCGT510.27548209366391185No Hit
TGTAATGGGCAGGCCTGACGGATGACACCCACACAGACCCTGTGTCCCAGGT500.27008048398422735No Hit
CTTGTAGGGTATTACTCCATTACTATTAACTATCGGAAAAATGAACTGGAAC500.27008048398422735No Hit
AAGGCAGGACTAATACTGAGTGATTGCAGAGTGCTTTATAAATATCACCTTA480.25927726462485823No Hit
TGGTCAGGGCGTGCCCCAGCCTGGACCCCTGGGGCTCAGATAGAGGTGCTGA480.25927726462485823No Hit
TCTGCAGGGATTATGATAATCATATTTACCAAATGCCCCTCATTTACATAAA480.25927726462485823No Hit
AGATATGGGTGCCCTGTGTGACAGGCACACCAAATTTTTTGAAGAGCCGGAA470.25387565494517367No Hit
GAAGCAGGGAACTTCTTTTCAGTTAGCATTATACTTGATATATCTATTCCAT470.25387565494517367No Hit
GCTACTGGGGGGTTCGAACACGGCACCCGCACTGCGCGTCATGGTGCAGGCC470.25387565494517367No Hit
GGATATGGGATGAGCAAATATGTCAAGGGAGTCAATTTCTGACCAATCAAGT470.25387565494517367No Hit
AAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGGAGCTATCTC430.23226921622643545No Hit
GACCATGGGGGGGGGCGCGCGCGCGGGCCTGGGGAGCTCGCGGGGAGAGCCC430.23226921622643545No Hit
GTAAAGGGGATTTGTGCCAATTTCTGGTTGGAATGGTGACAACATGCTGGAG420.22686760654675092No Hit
GGCATTGGGAAAGAACCAAAACCATCCCTGAAATGCCTTGCTAATACAACTA420.22686760654675092No Hit
ATCTTGGGGCCGATCAAACTGTGGGAGTGTTTGTTTTTGCACACCATTTTGT420.22686760654675092No Hit
CTTATACACATCTCCGAGCCCACGAGACAAGGAGCTATCCTGTCTCTTATAC420.22686760654675092No Hit
CATAGCGGGACATCAGGCAGAGGAGAGTGCAGCCTCACAGTGACTTTCTCAG410.2214659968670664No Hit
TGCGTAGGGATACCAAGGCCACCACACACCACCTGTCCAAAAAGGCCTTCGA410.2214659968670664No Hit
CGTAAAGGGGGATGACGAATTTGAATGCTATCTAGTGGGTCAGGGGAAGTCA400.21606438718738183No Hit
TGCCGCGGGATTGAAGAAAGTTTTCTTTGCCTTGCTTTGTTTTGCCTTACCC390.2106627775076973No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACAAGGAGCTATCCTGTCTCT380.20526116782801276No Hit
TTCTTAGGGATTTGTAAAATCAACTTTTCAAATAATGTAAATGTAATATACT380.20526116782801276No Hit
TGACAGGGGTCCGACTCTTCCTGGGAGAAGGGCAATGCTTTTAGATTAAAAT360.19445794846864364No Hit
AGTAAAGGGTTGGTTGGACACAGCGCTTAGTGCACGCTGTCATCATGGACAT360.19445794846864364No Hit
CTAGCGGGGGTCGTACGTGCTGTGCTACGACCCGCGCACCCGCGGCGCGCTC360.19445794846864364No Hit
AAAGCAGGGAGCATATGCATTTCCCCACCGCGTTGTGTCTGCAGCTTCTTTG350.1890563387889591No Hit
TTTTGTGGGGATAGGATACATCTTGGACATGGAATTGTTAAGCCACCTCTGA350.1890563387889591No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGGAGCTA350.1890563387889591No Hit
CTTTGGGGGGGTGGCCTGGGCGGCGTTGGTCCGGTGCGTCCTGTTCTACAGC350.1890563387889591No Hit
AAAGCTGGGCCTAGCAAAAAGTCTGAAATTTATCTTTCTTACAAACTGTTCC350.1890563387889591No Hit
ATTTTAGGGAAGCTTGAAGGCATTGGAATAGTAAAATATTATGAAACATTAC320.17285150974990549No Hit
TAGGTTGGGAGCCGGGGACATCGTCGGGCTTCCCGGGACCGAAGTGCGAAAT320.17285150974990549No Hit
AAACAAGGCATTCCAGTGGAAGACGAATGTAACCTGCGTGGTGATGTGACAA320.17285150974990549No Hit
TATTCGGGGAGACCATGTGTTCACGGGATGCCCGCACAAAACAGCTGAGGCA310.16744990007022093No Hit
GCTAGTGGGTAATCACAGCAGTCCTACTTCTCCTATCTCTCCCAGTCCTAGC310.16744990007022093No Hit
CTTATACACATCTCCGAGCCCACGAGACAAGGAGCTACTGTCTCTTATACAC310.16744990007022093No Hit
CCCAACCGGGGAATAACAACTAGAAAAGGCAGTATACTTCACTGATGCTTGT310.16744990007022093No Hit
TTGCCAGGGCATTTTAAAATCTTTTGCCTATACTTTGATATATACACCTTGG300.1620482903905364No Hit
TCATATGGGGGACTAACCCCTATACCTTCTGCATAATGAATTAACTAGAAAT290.15664668071085183No Hit
ATAAAAGGGAGGGCCCGCCACCCGAGGGTCGCGCAGCCCACTGGGCCCGATG290.15664668071085183No Hit
TATCGAGGGGAGGGTCTGGATGCCCACTTTCCATGCAGCAGCATTTCATTGC290.15664668071085183No Hit
TTGCAGGGGGCCAAAAGACATCTTACACAATCCACCTTTAACTTTTCAAGTT290.15664668071085183No Hit
ACTGCCGGGTCTCGCACTTCAACTGGCCGGTGCAGGGCGCCAACTACCACGT280.15124507103116727No Hit
CAGAATGGGACTGGGAGTGCTACCAGAACTACCACAGGATGAAAGTGGTGAG270.14584346135148274No Hit
TTGGCTGGGCGAGTGTAGCCCTGTTTCACTCGGATCAGGTTGGCACGGCCGC270.14584346135148274No Hit
AGTCCAGGGAGGCCTGCAAGGCCCAAGTGGACGCTCGGACAAGGGCAGGAAG260.14044185167179818No Hit
AGCAAAGGGAGTAGCCCTCTGGGCCTGGGATATTGTGGTTGATAACTGTGCC260.14044185167179818No Hit
TCAACAGGGATTCCTTCAAATATGTTTTCTGTGTTGTATACTTTTTCTCCTC260.14044185167179818No Hit
TGACTAGGGACTGTAAGACGTATGTAACATGATGTTAACTTTGTGTGGTCTA260.14044185167179818No Hit
ATCTGAAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGGAGCTAT250.13504024199211367No Hit
ATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGGAGCTATCTCGT250.13504024199211367No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACAAGGAGCTAACTCGTATGC250.13504024199211367No Hit
TTGCATGGGTAAAAATAGAATTGCCTTAAAGGATTTGAAGTAAGAACATAAT250.13504024199211367No Hit
TGCTGAGGGCAAGCCAAATACCAGATCCGCTACCGAGTGGAGGACAGTGTGG250.13504024199211367No Hit
GTTAGAGGGGCAGATAATTTTTAGCTTAGGGCCTGGTGGCTAGGTCGGTTCT240.12963863231242911No Hit
GTACATGGGACAGGACAACTGGCAAGTTTGCTGGGAAGTCAAATAATACATT240.12963863231242911No Hit
GCTAAGGGGAATGGGCTCAAATTCTCTACCTCTTCAGGGCTAATACTTTTAA240.12963863231242911No Hit
TTCATTGGGGCGGCTGCACCAGCTGGCCGCGGGCGCCAGGCTGGAGCCGGCC240.12963863231242911No Hit
ACTCTCGGGAGCTGACCCCCTCCCCCCAATCCCTCAAACCTTGAGGAGAAGT240.12963863231242911No Hit
TTACAGGGGGGTTTCTAAGAGCCTTTCTTGCAGCTAATTCCATAAAGTGTCT230.12423702263274455No Hit
TCGTAAGGGGGAGCTGGGGTGGCCCGGGGAGGCGCGGGCGGCGGCCCCAGCG230.12423702263274455No Hit
TCTTGAGGGCAGCAGAGTACAGAAGGGTGAAGAAGTGAGCAGAGGCTTATGA230.12423702263274455No Hit
GCTTGTGGGACTGCCTAATTTTCTCACCCACATTCGCTGAAGCGATGGAGAG220.11883541295306002No Hit
CAGCAGGGGTGCTCAGACACATTTAGGTTCAGGGTGGACCTTTATGTCTTGT220.11883541295306002No Hit
AGCGCCGGGAGCCTTCCGCCCTGGTATTTGGGGGGATGACCAGCCCAGGCGT220.11883541295306002No Hit
GGCTAGGGAGATCTGTCCTAGTGATTTTACCTTTGTTCTAGAAGGCGCTCCT220.11883541295306002No Hit
ATAATAGGCCAGCACCGCTGCCCTGTCCACCTGGCTGGCAGCTGCTTCACAG220.11883541295306002No Hit
CGTGAGGGGGAGCTGTTTAGTAATAACCCAGTTTTCTTGAGGTCTGTTTACT210.11343380327337546No Hit
GTATACGGGAAGCTAGGAGCCGCGCGAGTCGTAGTGTCGCTGTTTGCGGGTC200.10803219359369091No Hit
ACGGCCGGGACCGCGCCGCGGAAGGGGGCTCCGAGGGGGCGCAGCCTCAAAC200.10803219359369091No Hit
GCTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGGAGCTATCTCGTAT200.10803219359369091No Hit
CAATAAGGGAAGTGAAGTGATGGCGAATCCTCCCCAAGTCGAGACCGTGACC200.10803219359369091No Hit
TCTACCGGGCTTCCTGCGTCTCTGCAGCTTCCGCAACGCCAGCGAGGTGCCC190.10263058391400638No Hit
TGTTATGGGCAAATATTTATACACTACAAAAGATTAAAAAAGGAGAGGGAGA190.10263058391400638No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACAAGGAGCTACTGTCTCTTA190.10263058391400638No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)