FastQCFastQC Report
Sat 31 Dec 2022
SRR5846854.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5846854.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences151051
Sequences flagged as poor quality0
Sequence length52
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAAGGAGCTATCTCGTATGCCGTCT11470.7593461810911546RNA PCR Primer, Index 38 (95% over 21bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACAAGGAGCTATCTCGTATGC10250.6785787581677711No Hit
CACACGAGGGCAGAAAGGAAAGTATAGAAGAAGGAAACTTGGAGTATCAGAA2090.1383638638605504No Hit
GCAAATGGGGGAACAAATGTGATCTGACCACAAAGAAAGTAGTAGACTACAC1660.10989665742034146No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)