FastQCFastQC Report
Sat 31 Dec 2022
SRR5846851.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5846851.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences83198
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAAGGAGCTATCTCGTATGCCGTCT7680.9230991129594461RNA PCR Primer, Index 38 (95% over 21bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACAAGGAGCTATCTCGTATGC5260.6322267362196207No Hit
AGATGTGGGGAATCGTTTAATTTTCTGTAAGGTAATGATGATCATTTGAATG1400.16827327579989904No Hit
ATACGCGGGTATGGATGAAGAAAAATAATTAGGCATGAAAAATGTAGATAAA1360.1634654679199019No Hit
CATCAAGGGTGTGCCTTCCTGTGCTCCGGCCACAGGAGCGTCGGCCCAGGGG1290.15505180412990696No Hit
TGATGCGGGACAAGGGATGAGTACTTTTTAGAGTATGTGCATGTTACGTAAT1290.15505180412990696No Hit
AGACTGGGGATATGAAGACTTAGAAGAGTAGCATGAGGAAGGAAAAGATAAA1220.14663814033991202No Hit
GCTGGTGGGGCAAAATAGATAAGTACAACACTCCATTTAGTGTTTTGCCAGA1220.14663814033991202No Hit
GTCAAAGGGAACTGAGTGTGGTGGCTTATGTCTGTAATCTCAGCCTCCCCAA1180.14183033245991492No Hit
GTGCTTGGGCCGTCATCAGGGACTACCTGGTCCAAAAACTACAGAAACAGAG1170.1406283804899156No Hit
CATAAAGGGATTTTAGTCTTTTCTACGATAAAAATTGTATGTGTACTTTCAT1150.13822447654991707No Hit
GAGTGCGGGTACGGTGGGGTGAACAAGGAGGGCTTGGAATGCCTTCGGCTCC1150.13822447654991707No Hit
ACAGCTGGGGGGCCCCGAAGGGCACTGTGAGGGACAGAGGCTCACCTGGGCA1150.13822447654991707No Hit
GTCAAAGGGTTAGACAGATCAACAAGACAGAAGATTAACAAGGATATCCAGA1150.13822447654991707No Hit
CCTCGAGGGCAAGTTCCCTGAGGCACCTACTGTCAAGGCCTCCTGTGGCTTC1140.1370225245799178No Hit
AAGCCAGGGAGGCGAGAAGCCGTTCAAGTGCTACAAGTGCGGCCGTGGCTTC1140.1370225245799178No Hit
CTATTGGGGTAATGATCACACAGGCAAGGGCCACACATATCACTGGCATCCT1140.1370225245799178No Hit
GTCTAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1140.1370225245799178No Hit
GTAGAAGGGCAGGGATTATAATTAGTTTACATACATACTCCTTAAACAGATA1130.1358205726099185No Hit
CGTCCTGGGTCACCATCAAGATTAGATATCTTTGATTAGTTTTGGTAACTTC1130.1358205726099185No Hit
CTGCCTATTGAGACAACCAGGTGCATAATTGATTGCCCTTTGGCCATAAAAA1130.1358205726099185No Hit
GGCAATGGGCACTTGCCTTGTTGCTGAAGTTCCAATAATGTGAAAACCAAAG1120.13461862063991922No Hit
ACTTGAGGGAGTCTACTGCGTATTTCCCTGCTCTCTACAGATACCTTATCGC1110.13341666866991997No Hit
TTCCGCGGGAGGTAGAGGAAACTGACTCTCGATTATGTGTAAGAACAGTGGA1110.13341666866991997No Hit
GGAACAGGGTGGGTGGCTGAGGGGATCGTTAATTGAATGGGGCAGACTGAGG1110.13341666866991997No Hit
ATGGTAGGGTGAGATGAAAGATCTTGCCATTCAAAAGAGGATCAGAGTGGAA1100.13221471669992066No Hit
TGCCAGGGGCGGCCTGGCGGCCCCAGGACGCAGCAGCAACCTTGGGCTTCCT1090.1310127647299214No Hit
CAGTATGGGAACAAATTGCCACTTCCACCAAGGCTAAGGAAAAGCAAGATAA1080.12981081275992212No Hit
AACACGGGGGAACATCCATTTGCCCTATAATACTTCTTAATTTTTCCCAGAC1080.12981081275992212No Hit
GAGAAAGGACATCCACACCAAAACCCCATCTGTACGTCACCATCATCAAAGA1080.12981081275992212No Hit
GTGCAAGGGTGAAGGCTGAATCAAAGACATTTCATCCACCAATATCATGTGT1080.12981081275992212No Hit
ACTTTAGGGCTAATTGCCCACAAGAGAAAGCAGGAAAGATCTAAAATCAACA1070.1286088607899228No Hit
ACCGCTGGGACCAGTGCAACAGCATCTTCAAAGAAAACAATTGAAGATAGAA1070.1286088607899228No Hit
GTTAGGGGGGTTGTTAACTAGATAGCTGTAACTCCAAAAAGACAACTTAAAT1060.12740690881992356No Hit
TAATCGGGGGTGATAGTTTTCATTAAATTTGGAACAATTTTGACCATCACTT1060.12740690881992356No Hit
GAAAATGGGATTGTGAACATAATTTGAACTCATTATCACACACTTGGAAAAT1050.12620495684992428No Hit
CACGTCTGGGAGCTATTATTAGTGTCAATGTAGTATTTAGTATACAGTTAAT1050.12620495684992428No Hit
AAGCCAGGGCTTTTGATTGATAAATAATATTTCAGCCTTATTTTTGTAAGGA1050.12620495684992428No Hit
TCAACAGGGTTATCAACTTAAGGAGATTTTGGGCTGAGACGATGGGGTTTTC1050.12620495684992428No Hit
GTTGCAGGGAACAGTCTCCGCGGACAGACTCGCATCAGCCGCCCTGGGGCTC1040.125003004879925No Hit
ATATATGGGCAAACGCAGAAGGACTGCCTCACTGAGCAACCAAGAGTGTCAG1040.125003004879925No Hit
TTAGATGGGATTTGTATCCTTTCTTGTTCTTGTGATCTTGTGATGAAGACTT1030.12380105290992571No Hit
TAACTTGGGATGTTGTGCACTAAAGCAATTGTTCTCTTATCGTAGAATTTTG1030.12380105290992571No Hit
ATAACGGGTAAGATGATAATCTTAGTTTATTCAGGCTGCTGTAACAAAATAC1030.12380105290992571No Hit
TACAAAGGGGGTTGCCTGCTGCAGTGTCAGTTTCTACTACCTAGCAACCAGC1030.12380105290992571No Hit
CAAGCTGGGGAGGCAAGGAAAGACCAGCAAAGAGACAGAGAAGAAAGAGACA1020.12259910093992644No Hit
AGTCATGGGAGCAGGCTTTGGAAGTGATAAAGCAGTTAAAAGAGAAAATGAA1010.12139714896992716No Hit
AGTCATGGGATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTA1000.12019519699992788No Hit
AAGCTAGGGATGAACTTTGGTTCTTCATGAGTGCATTTTGAAAAAGAAAAAA990.1189932450299286No Hit
GTTAGGGGGTGACTTTTCCAAAAAGGAAACATGAAAACACTCTCATATTCTT990.1189932450299286No Hit
CCGCCGGGAGGCTGATAAGCAATTTCAGCAAAGTCTTAGGATACAAAATCAA990.1189932450299286No Hit
TAGCATGGGCTGGTTATGAGTCACTGTTTTTTTCTCAATGTTAAAATGAGTG980.11779129305992933No Hit
GTAAACGGGGCTTTGGTTCGCCTTTGTTAATGGATTTTCTGGGCAGATTTTA980.11779129305992933No Hit
GATCGCGGGAGCCTTGTGTGTTATATTACTCGATTTACAGAAGAGGCTTTTG980.11779129305992933No Hit
GGTTTTGGGAGAGATTGTCTTTCCTGTTGGAAAAGGCAATCACTAGGGAAGC970.11658934108993003No Hit
GTCTTGGGCTCTTCCGATCTGCCAGGGGCAGTACAGCCTTTCAGTAAAGAAG970.11658934108993003No Hit
GAGTGAGGGGCCAGCATTCTGGAGTCACAATAATTAAATAAAATGTAAATCC970.11658934108993003No Hit
TCGTACGGGGTACGAATTGGAGCAATGTTGGCTTATACACCTCTGGATGAGA950.11418543714993148No Hit
TTGGCAGGGGGGTATCTGGCAGAGCAAGATCTAGGCAGGGAAATAGTGCCAA950.11418543714993148No Hit
GCTTCAGGGCGGCTCACTGCAGGCTCCGCCTCCCGGGTTCACGCCATTCTCC940.11298348517993222No Hit
GCTAGAGGGGGAAAATAGGACACATTATTAGCCCAGTTCTAAATCCAGTTCC940.11298348517993222No Hit
CCGATAGGGAAGTCCTGTCACCAAGGACGTTAAAGGCATTTTATTCCAGCGT930.11178153320993292No Hit
TGTCTTGGGGAATGCAAACAATTCTTAGGACATTTTTAATATCATTTTACCA930.11178153320993292No Hit
CGCAAAGGGGACCTCACAGACAGCAGCAGCTTTGAGCGAGCAAAGTTCTGGG930.11178153320993292No Hit
ATGAATGGGATGTGGGTGTCAGGATGAAAGGAAGAAATCTAGATTTGATGTG920.11057958123993365No Hit
GGCGCTGGGATCTCTTAACTTTTATCTTTATTATTTTACAATGCCCTTGTAA920.11057958123993365No Hit
GCTTCAGGGACAGTTTAATTTACTACTTCTAAGACAATGCTGAGACCCTATA920.11057958123993365No Hit
CAGAATGGGGAGAACATGAAGCGCAAACAGAACGACGTCTTTGGAGAAGCTG920.11057958123993365No Hit
AGCGGCGGGAAGAGAGTTAAAATTTAAAAATGCATCTTGGGAGAGAAATTTG910.10937762926993438No Hit
CAACAGGGAGGGTAACAAATGATGCCCGAGAAAATGAAATGGATGAAAACCT910.10937762926993438No Hit
GGCTTGGGGAACGCAAGTTCCCAGTTGGAGCTCCAGCCTGACATCAAAAAAG910.10937762926993438No Hit
ATTGTTGGGGTACTTCACTAGTGCTGCCAGAGGAATTGTTAATAAAAGCACC910.10937762926993438No Hit
AGTTCCGGGGCAGAGGAAAACAGCATGTGGGTATTTGTCGACCAAAATGATG910.10937762926993438No Hit
AGCGCTGGGATGGCTCCCTCTGTTGAGATTGGAATTGGAAGAGAACTTAATT910.10937762926993438No Hit
TGGCCGAGGGATCTGAATCTGTCTCCCAGAATAAAATACTACTTCAAAGGAT910.10937762926993438No Hit
AAACATGGGGCAAAAGGAAGTTTTCATCTTTGAACATTAAAGGTATCCTTTA900.10817567729993509No Hit
GCTGATGGGGGGATGTCAAAGTTGTCCAAAAATGGTTTTATTGGGCAGTGAA900.10817567729993509No Hit
GTTAAAGGGTGTAACCTAAATGACACTGGTGTTAGGACCTGAGACCTTAAGC900.10817567729993509No Hit
TGAAGCGGGCTGGCATCAGCCTCATACCTAAAACTCCTGACTCTTACCACTC890.1069737253299358No Hit
TAAGGGGGATGTTCACATTTGATAAGTATATGCCAAAAAATGAAGCCGGGAA890.1069737253299358No Hit
GTGCCTGGGTGGTGATAATGATGGTGATGGTGATAATGATGGCGATGATAAT890.1069737253299358No Hit
AGTTTCGGGTATGTGATTGTTCTTAAAGTCAATGTATTAAATAAGGTATTTT890.1069737253299358No Hit
ACACGTGGGATGGGAGCTGTTTATATAGATCAACTACATTGTGGGACAAATA890.1069737253299358No Hit
CGGTGCGGGACTGAGAAAGTATTGTAAGGCACCATTTATTATTCACCTTTGT890.1069737253299358No Hit
TCCATGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA880.10577177335993654No Hit
TAGTTGGGGATGTATAGAGGTTGTCATAAAGCCAGGCTTGTTAACATGAACA880.10577177335993654No Hit
AGGCAAGGGAATATTACCCTTTCCTATGTGTTTTATCTAATTATTTTGGTTG880.10577177335993654No Hit
TACCCCGCCTGTTTACCAAAAACATCACCTCTAGCATCACCAGTATTAGAGG880.10577177335993654No Hit
CTCGCTGGGAATGCCCCAACTAAATACTACCGTATGGCCCACCATAATTACC880.10577177335993654No Hit
ACAACTGGGAACAAACGGGGGCGCATAGCTATCACCTGTAGCTCCCTCCCTA880.10577177335993654No Hit
TTATATGGGATGTAAGTTGTGCCGTCATTTGTTTTGCTTAACAGTTTTTTTG880.10577177335993654No Hit
ATATGGGTGAGTATTTCCAGGACAACATTCTAAAAGTACAATTATTTCTTAT880.10577177335993654No Hit
TTAGCAGGGCATTTCTCCTGTTACAATTAAAGTCTTTTTTTCCTATTTTCAG880.10577177335993654No Hit
ATGTATGGGACCAAAACTGTATCTAATATCTATTATCTTTAGGATGAGTAAT880.10577177335993654No Hit
ATAACAGGGATATGTCAATTTAAAAAAATAAAAGTCTGCTTTGTAAATAGTA880.10577177335993654No Hit
GCGACCGGGTTCTGGAGAGATTCTGTATAGTCACGTGTTAACCCTCTTTTCA880.10577177335993654No Hit
CTCTACGGGAAGCCAGGGCTGGAGAACAAGAAAGGGCCAGGAGATGGAATTC880.10577177335993654No Hit
AGACACGGGTACACACCATCAGCTTGTAAGTGGTCACAATTCAATTTTGCAA880.10577177335993654No Hit
GCGCATGGGAACGTCAGAAATTCATTACATTAAAAATGTGAAAAATGTTGCA880.10577177335993654No Hit
ACTTAGGGGTTAGTGTTTATGATGTTTTTATCATAAAGGATGTCAGAGAAAC870.10456982138993727No Hit
GACTAAGGGGACAGAAAAGACATCTAAGAAAGAAAACTAGCAAATTTATCTT870.10456982138993727No Hit
TTACCAGGGAATGTTAGTATAAGTAACATGAAAACATTCTCCTCCGCATAAG870.10456982138993727No Hit
TCTAAGGGGATTCTTCAGGTAGATAATTTTTCAAATGTGAATTATCTTTTGT870.10456982138993727No Hit
TGCGCTGGGTACACGACGCACTTCCCACATATAGATACGTGTTTAGTGACAG870.10456982138993727No Hit
ATGTGAGGGGGCAGACTAGCTGGGTGGCTAGACCCAGAAGAGAGACAATAAT870.10456982138993727No Hit
GTTTATGGGACTCTCACCCACGGAAAGCTTCTTTTCATTAGTAGTAATGGAA870.10456982138993727No Hit
CCAAGTGGGAGTGTCAATGTTTGTCAAAATGTGACTCTTTGAATATTCTAAT870.10456982138993727No Hit
CCAATGGGTCTTAGGACGGGAGATGTGATGAGCTAGCAAATAGCATGTAGGA870.10456982138993727No Hit
TTTTCTGGGGTCCTCCTCATTGGAATTCACTATGAGTCAGAATCATTAGACT870.10456982138993727No Hit
ATGTGGGGGTTTGCAGAAACATCTAAGAAGCGCTGAATTCTAGTTCAAGCCC870.10456982138993727No Hit
AAGTGCGGGTCAGCAGTCTCTCTGGAGAATCAGAAGAAACCTATTTGTCATT860.10336786941993797No Hit
GTCTGCGGGACAGTTCCAAAACTGAGCCAGACCAAATTTATCCTGCTACAAA860.10336786941993797No Hit
TGGAGAGGGAAAAGTTTGGGAAGGAGTGGGCCCATGCCACAATCATCCCCAA860.10336786941993797No Hit
CGTTCTGGGAGATGTGGAGTTGTGGGAATTCTGGGGAAGTTTCCAAGTTGTC860.10336786941993797No Hit
TAAAGGGGGATCTAAAACGCGGGCGGTGCAGGAGCTGAAGGTGAGTGGAGTG860.10336786941993797No Hit
TGCTCTGGGCTCTGGGAGGGGTGCTGCCTTGTAGATGGAGCAAGTGAGCACT860.10336786941993797No Hit
CACATGGGGGGAGCTACCTGTGGGGACCTGAGCCATCACTATTCCTGCTTCA860.10336786941993797No Hit
TACCAGGGGGCACACAGCAAGTGACAGAACCAGGGCTTCAGCCTGGGATTGT860.10336786941993797No Hit
TCAATAGGGCAACACCATCAACCAACAGATCTAATGAACATTGCTAAAGTGC860.10336786941993797No Hit
TTGGCCGGGGTTGAAGGGACTTTGTTGGCTTTTAGAGTGTTGGTCATGACTC860.10336786941993797No Hit
TTCCTTGGGAATAATGGAAGTACATACTTGGAAGATGGCTGGGTTAAGAAAT860.10336786941993797No Hit
CCGTCCAGGGCCCCACATCGATGAAAGCAAAGCGAAGCCACCAAGCCATCAT860.10336786941993797No Hit
CGGCGTGGGTGTCTGGGACACTGACCTGTCAAAACTGGCCCCTGGCTCACTG850.1021659174499387No Hit
CATTAGGGGAGAAAAGGACAAGTGATGACTTGGTAATAGGAAGCAGTGCAGA850.1021659174499387No Hit
TTGTCGGGGGCAAAGAGGCAGTTCTTCTGGGTCTTGACAGCAGATGTTGGAC850.1021659174499387No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGGAGCT850.1021659174499387No Hit
GCTGATGGGACACTTGAGCAGCCTTAGCTAGACTGACCAGGGAGAAAGAGAG850.1021659174499387No Hit
TCACAGGGAGACATATTCTTGGATGATATTTTAGCATTATGTATTTATTCAG850.1021659174499387No Hit
TCTAATGGGATGTCTGTCAGCCAGTAAAGATTAAGAACAGAGTTTCTCTAAA840.10096396547993942No Hit
ATTAGAGGGCTGTTACTAATGATGGGGAAGAAACCAAAACTACTGAGGAATC840.10096396547993942No Hit
ACTGGAAAGGGCATTGGAGAAAACAGGTGTGTATAAGAGTACAGGAAAACAG840.10096396547993942No Hit
CACCGCGGGGTACGACCTGTCCAAAGGCCTGGCCCGGCGGCTCAGCCCCATC840.10096396547993942No Hit
AACAGTGGGCAGATCCTGGGATGGTTAACGAAGGCACAAAATGTGGTGCTGG840.10096396547993942No Hit
AGTATCGGGGAAGGTGGTTTATTACAAATAAGCACACTAACCCCTCGGAAGT840.10096396547993942No Hit
TGTGATGGGCGGGAGCCACGGCTTCTTGTGGTTACTGACCCCAGGGCTGACC840.10096396547993942No Hit
AGCATAGGGTACTAAAAATACAAAATTAGGTGGGCGTGGTGGCGCATGCCTG840.10096396547993942No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)