FastQCFastQC Report
Sat 31 Dec 2022
SRR5846850.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5846850.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences84297
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAAGGAGCTATCTCGTATGCCGTCT7780.9229272690605835RNA PCR Primer, Index 38 (95% over 21bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACAAGGAGCTATCTCGTATGC5310.6299156553614007No Hit
AGATGTGGGGAATCGTTTAATTTTCTGTAAGGTAATGATGATCATTTGAATG1570.186246248383691No Hit
GAGTGCGGGTACGGTGGGGTGAACAAGGAGGGCTTGGAATGCCTTCGGCTCC1480.17556971185214182No Hit
GTCAAAGGGAACTGAGTGTGGTGGCTTATGTCTGTAATCTCAGCCTCCCCAA1370.16252061164691506No Hit
CATAAAGGGATTTTAGTCTTTTCTACGATAAAAATTGTATGTGTACTTTCAT1360.16133432981007628No Hit
AGACTGGGGATATGAAGACTTAGAAGAGTAGCATGAGGAAGGAAAAGATAAA1360.16133432981007628No Hit
GCTGGTGGGGCAAAATAGATAAGTACAACACTCCATTTAGTGTTTTGCCAGA1360.16133432981007628No Hit
AAGCCAGGGAGGCGAGAAGCCGTTCAAGTGCTACAAGTGCGGCCGTGGCTTC1340.15896176613639867No Hit
GTGCTTGGGCCGTCATCAGGGACTACCTGGTCCAAAAACTACAGAAACAGAG1290.1530303569522047No Hit
ATACGCGGGTATGGATGAAGAAAAATAATTAGGCATGAAAAATGTAGATAAA1270.15065779327852713No Hit
AAGCCAGGGCTTTTGATTGATAAATAATATTTCAGCCTTATTTTTGTAAGGA1270.15065779327852713No Hit
GATCGCGGGAGCCTTGTGTGTTATATTACTCGATTTACAGAAGAGGCTTTTG1220.14472638409433314No Hit
CTATTGGGGTAATGATCACACAGGCAAGGGCCACACATATCACTGGCATCCT1210.14354010225749433No Hit
ACAGCTGGGGGGCCCCGAAGGGCACTGTGAGGGACAGAGGCTCACCTGGGCA1210.14354010225749433No Hit
CGTCCTGGGTCACCATCAAGATTAGATATCTTTGATTAGTTTTGGTAACTTC1190.14116753858381675No Hit
CATCAAGGGTGTGCCTTCCTGTGCTCCGGCCACAGGAGCGTCGGCCCAGGGG1190.14116753858381675No Hit
GGCAATGGGCACTTGCCTTGTTGCTGAAGTTCCAATAATGTGAAAACCAAAG1150.13642241123646154No Hit
AAGCTAGGGATGAACTTTGGTTCTTCATGAGTGCATTTTGAAAAAGAAAAAA1140.13523612939962276No Hit
GTTTAAGGGGGAGCTAGGCTGGGACAAGCCACTGATTTGAACAAAAAGACAG1140.13523612939962276No Hit
GTCAAAGGGTTAGACAGATCAACAAGACAGAAGATTAACAAGGATATCCAGA1130.13404984756278396No Hit
AGCGCTGGGATGGCTCCCTCTGTTGAGATTGGAATTGGAAGAGAACTTAATT1130.13404984756278396No Hit
TAGCATGGGCTGGTTATGAGTCACTGTTTTTTTCTCAATGTTAAAATGAGTG1100.13049100205226757No Hit
AGACACGGGTACACACCATCAGCTTGTAAGTGGTCACAATTCAATTTTGCAA1100.13049100205226757No Hit
ACCGCTGGGACCAGTGCAACAGCATCTTCAAAGAAAACAATTGAAGATAGAA1090.12930472021542877No Hit
GAGTGAGGGGCCAGCATTCTGGAGTCACAATAATTAAATAAAATGTAAATCC1090.12930472021542877No Hit
CAAACCGGGAAGGAGACGAGAGGCCCACCATCCCGGACCCAAGTCTTCTTCC1080.12811843837859No Hit
GTTAAAGGGTGTAACCTAAATGACACTGGTGTTAGGACCTGAGACCTTAAGC1080.12811843837859No Hit
CCGCCGGGAGGCTGATAAGCAATTTCAGCAAAGTCTTAGGATACAAAATCAA1070.1269321565417512No Hit
TTCCGCGGGAGGTAGAGGAAACTGACTCTCGATTATGTGTAAGAACAGTGGA1060.12574587470491239No Hit
CCGATAGGGAAGTCCTGTCACCAAGGACGTTAAAGGCATTTTATTCCAGCGT1060.12574587470491239No Hit
TGTGATGGGCGGGAGCCACGGCTTCTTGTGGTTACTGACCCCAGGGCTGACC1040.1233733110312348No Hit
AGTCATGGGATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTA1030.122187029194396No Hit
GGACTGGGAGAAGAAAAGTGATTAGCTAGCATGCGAAGCAGGAAGCTACAAA1030.122187029194396No Hit
GAGAAAGGACATCCACACCAAAACCCCATCTGTACGTCACCATCATCAAAGA1030.122187029194396No Hit
ACACGTGGGATGGGAGCTGTTTATATAGATCAACTACATTGTGGGACAAATA1030.122187029194396No Hit
ACTTTAGGGCTAATTGCCCACAAGAGAAAGCAGGAAAGATCTAAAATCAACA1020.12100074735755721No Hit
AGTTTCGGGTATGTGATTGTTCTTAAAGTCAATGTATTAAATAAGGTATTTT1020.12100074735755721No Hit
CAAGCTGGGGAGGCAAGGAAAGACCAGCAAAGAGACAGAGAAGAAAGAGACA1020.12100074735755721No Hit
GGAACAGGGTGGGTGGCTGAGGGGATCGTTAATTGAATGGGGCAGACTGAGG1010.11981446552071841No Hit
TTGTCGGGGGCAAAGAGGCAGTTCTTCTGGGTCTTGACAGCAGATGTTGGAC1000.11862818368387962No Hit
CTTTCCGGGAACTGATGAATAATTGAAATGATTTTTATATTTTTTTGTTTAA1000.11862818368387962No Hit
GTCACAGGGGCAACAGAGAAGATCAATTAGAACTCGTTCTGAATCAGAGAAA1000.11862818368387962No Hit
TTGGCCGGGGTTGAAGGGACTTTGTTGGCTTTTAGAGTGTTGGTCATGACTC1000.11862818368387962No Hit
ACTGGAAAGGGCATTGGAGAAAACAGGTGTGTATAAGAGTACAGGAAAACAG990.11744190184704083No Hit
TCGTACGGGGTACGAATTGGAGCAATGTTGGCTTATACACCTCTGGATGAGA990.11744190184704083No Hit
CTGCCTATTGAGACAACCAGGTGCATAATTGATTGCCCTTTGGCCATAAAAA990.11744190184704083No Hit
ACAACTGGGAACAAACGGGGGCGCATAGCTATCACCTGTAGCTCCCTCCCTA990.11744190184704083No Hit
CACGTCTGGGAGCTATTATTAGTGTCAATGTAGTATTTAGTATACAGTTAAT990.11744190184704083No Hit
GACTAAGGGGACAGAAAAGACATCTAAGAAAGAAAACTAGCAAATTTATCTT980.11625562001020201No Hit
GTAGAAGGGCAGGGATTATAATTAGTTTACATACATACTCCTTAAACAGATA980.11625562001020201No Hit
CGGCCTCCCAAAGTGCTGGGATTACAGGCATGAGCCTCCGCACCCGAATAAA980.11625562001020201No Hit
GTTAGGGGGGTTGTTAACTAGATAGCTGTAACTCCAAAAAGACAACTTAAAT980.11625562001020201No Hit
TGATGCGGGACAAGGGATGAGTACTTTTTAGAGTATGTGCATGTTACGTAAT980.11625562001020201No Hit
ATATATGGGCAAACGCAGAAGGACTGCCTCACTGAGCAACCAAGAGTGTCAG980.11625562001020201No Hit
TGGCCGAGGGATCTGAATCTGTCTCCCAGAATAAAATACTACTTCAAAGGAT980.11625562001020201No Hit
GTTGCAGGGAACAGTCTCCGCGGACAGACTCGCATCAGCCGCCCTGGGGCTC970.11506933817336323No Hit
GTCTTGGGCTCTTCCGATCTGCCAGGGGCAGTACAGCCTTTCAGTAAAGAAG970.11506933817336323No Hit
TCAACAGGGTTATCAACTTAAGGAGATTTTGGGCTGAGACGATGGGGTTTTC970.11506933817336323No Hit
TGGCGCCGGGAATCTATGGGAAGAATATATAATAGTGACCTGTATTTTATGT970.11506933817336323No Hit
AGTTCCGGGGCAGAGGAAAACAGCATGTGGGTATTTGTCGACCAAAATGATG970.11506933817336323No Hit
ACTTGAGGGAGTCTACTGCGTATTTCCCTGCTCTCTACAGATACCTTATCGC960.11388305633652444No Hit
GTCTAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA960.11388305633652444No Hit
ATGGTAGGGTGAGATGAAAGATCTTGCCATTCAAAAGAGGATCAGAGTGGAA960.11388305633652444No Hit
ATGAATGGGATGTGGGTGTCAGGATGAAAGGAAGAAATCTAGATTTGATGTG950.11269677449968563No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGGAGCT950.11269677449968563No Hit
TAACTTGGGATGTTGTGCACTAAAGCAATTGTTCTCTTATCGTAGAATTTTG950.11269677449968563No Hit
GTTGTTGGGATACAGGGAAGAGTCATGATAGGAAACCAAAGGTATCCTGGTA950.11269677449968563No Hit
ATAACAGGGATATGTCAATTTAAAAAAATAAAAGTCTGCTTTGTAAATAGTA950.11269677449968563No Hit
CTCTACGGGAAGCCAGGGCTGGAGAACAAGAAAGGGCCAGGAGATGGAATTC950.11269677449968563No Hit
TTTCAGGGGTCCGGAGCGCGCACACAGATCCGGAGCGCGCACACAGATCCGG940.11151049266284684No Hit
ACGAACGGGAGTGCATCTGAAGCTTTTCATATAACGATGATGAAGACGGCTG940.11151049266284684No Hit
AAGCGTGGGCTTCTAAAGTGCTGGGATTACAGGCATGAGCCACTGCACCCCG940.11151049266284684No Hit
TCACTGCGGGAGCCTGCAATCGGTCTGATTGGTTGCAGAAAACAGCCAACCA940.11151049266284684No Hit
AATAGTGGGATCCATCATTTTAGTATAAGCACATTCATAACTGTTTAAAAGA940.11151049266284684No Hit
CGTAATGGGAGAGTGGGCAGTCCTTCTCCAGAGCCTCTGACCAGCTCAAAAG930.11032421082600805No Hit
TTAGATGGGATTTGTATCCTTTCTTGTTCTTGTGATCTTGTGATGAAGACTT930.11032421082600805No Hit
GCTTCAGGGACAGTTTAATTTACTACTTCTAAGACAATGCTGAGACCCTATA930.11032421082600805No Hit
TTAGCAGGGCATTTCTCCTGTTACAATTAAAGTCTTTTTTTCCTATTTTCAG930.11032421082600805No Hit
GGCGCTGGGATCTCTTAACTTTTATCTTTATTATTTTACAATGCCCTTGTAA920.10913792898916924No Hit
AAACATGGGGCAAAAGGAAGTTTTCATCTTTGAACATTAAAGGTATCCTTTA920.10913792898916924No Hit
CACATGGGGGGAGCTACCTGTGGGGACCTGAGCCATCACTATTCCTGCTTCA920.10913792898916924No Hit
CTAAGAGGGACCTATTCTCTTCAAGAAGAGATGTTGGCTGGAATTAGACATG910.10795164715233045No Hit
CGGCGTGGGTGTCTGGGACACTGACCTGTCAAAACTGGCCCCTGGCTCACTG910.10795164715233045No Hit
TAGAACGGGTGGACCCAGTATGTCACTTTAATGGACTTCAAGAAAAAATCTG910.10795164715233045No Hit
GTCTGCGGGACAGTTCCAAAACTGAGCCAGACCAAATTTATCCTGCTACAAA910.10795164715233045No Hit
GTCGCCGGGATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTA910.10795164715233045No Hit
GCCTAAGGGATAATCCACCATGATCAAGTGGGTTTCATACCAGGGATGCAGG910.10795164715233045No Hit
GGTGTTGGGACTTTGGGAGGCTGAGGTGGGTAGATTACTTGAGCCCAGGAAT910.10795164715233045No Hit
AGTCATGGGAGCAGGCTTTGGAAGTGATAAAGCAGTTAAAAGAGAAAATGAA910.10795164715233045No Hit
TGAAGCGGGCTGGCATCAGCCTCATACCTAAAACTCCTGACTCTTACCACTC900.10676536531549165No Hit
CATCCTGGGGAGAGAAGGATAATGAGTTTGACTTTACATAGAATGAAGGGCA900.10676536531549165No Hit
TAAGGGGGATGTTCACATTTGATAAGTATATGCCAAAAAATGAAGCCGGGAA900.10676536531549165No Hit
GCTAGAGGGGGAAAATAGGACACATTATTAGCCCAGTTCTAAATCCAGTTCC900.10676536531549165No Hit
GCATCCGGGAACTTTGCTTCTTTTAATATAGGCAGGGAAGTCAAAAGATTGG900.10676536531549165No Hit
CGTTCTGGGAGATGTGGAGTTGTGGGAATTCTGGGGAAGTTTCCAAGTTGTC890.10557908347865286No Hit
CGTTAAGGGCCCCACTTGCTCATCAAAGTAGTTCAACCAACATGCTTCTTTC890.10557908347865286No Hit
GTTCCAGGGTAAGCAAATTTCATTTCACATTTTTGTAATGCTGTACAGCAGT890.10557908347865286No Hit
CCGTCCAGGGCCCCACATCGATGAAAGCAAAGCGAAGCCACCAAGCCATCAT890.10557908347865286No Hit
GTCAAAGGGAATTGGAGATCTCTTTAATTTCGATCAACTTATAATGTGTAGT880.10439280164181407No Hit
CATTCCGGGTCCGATCTTTTAGCGGGATGTGTCATGATTTCAATGATTGTTT880.10439280164181407No Hit
TACCCCGCCTGTTTACCAAAAACATCACCTCTAGCATCACCAGTATTAGAGG880.10439280164181407No Hit
ATTGCTGGGATTACGGGCGTGAGCCACCGCACCCGGCCCAGAGCTTACTTTT880.10439280164181407No Hit
TTGACAGGGCAGATCATCCAGCTACAAATTCAACAAAAAAACATTGGATCTA880.10439280164181407No Hit
ACACGTGGGAAAAAGCAGTGGTCAAACAGAAAAGAAAGATTTAGTTGAACTT880.10439280164181407No Hit
TTATTTGGGATGTGCCTGCTGATGACTTGACTGACCCTGCCCCTGCTACTAC880.10439280164181407No Hit
TTCCTTGGGAATAATGGAAGTACATACTTGGAAGATGGCTGGGTTAAGAAAT880.10439280164181407No Hit
GTTTACGGGAAACTTGATAGATAATTCCCCAAATTTAACAACAATTTAAAGA870.10320651980497526No Hit
ATTCAAGGGGAACAAAGTGAGAAATGAAATCGAATGGAGATTGGCCTGGTTG870.10320651980497526No Hit
CCTCGAGGGCAAGTTCCCTGAGGCACCTACTGTCAAGGCCTCCTGTGGCTTC870.10320651980497526No Hit
ATACAAGGGTATCAGTGAACTTGCTGGGTGGTATGTATGGTAAGTGTATGTT870.10320651980497526No Hit
CCTACTGGGAAATGAACTTTGTTTGGACCATTCCACAAGCAACTTAAACTGC870.10320651980497526No Hit
TAAAGGGGGATCTAAAACGCGGGCGGTGCAGGAGCTGAAGGTGAGTGGAGTG870.10320651980497526No Hit
ATTAGGGGGGGGTTAAGCTTACCCAAGAGTGTTACTTCATTTCCAAGTCTAA870.10320651980497526No Hit
CACAACGGGGATTCGGTCGTGCTGGAGAATACAAAGGCAGTAATCAAAGAGC870.10320651980497526No Hit
GTGCAAGGGTGAAGGCTGAATCAAAGACATTTCATCCACCAATATCATGTGT870.10320651980497526No Hit
TGCTCTGGAAGCTTTATTTATTAGACGTGTTGAGTGAGTGCTGAGTTCCTTG860.10202023796813647No Hit
AACTCAGGGATCTGTGTCTGACCAAATCAGGGGACTTCCCCACCTGTGGTGG860.10202023796813647No Hit
GGTTTTGGGAGAGATTGTCTTTCCTGTTGGAAAAGGCAATCACTAGGGAAGC860.10202023796813647No Hit
GCGCTGGGTGAGGATGGTCTCATTCATAGACCTCTCAGACACGGTGGTAATT860.10202023796813647No Hit
AGTATCGGGGAAGGTGGTTTATTACAAATAAGCACACTAACCCCTCGGAAGT860.10202023796813647No Hit
CGCAAAGGGGACCTCACAGACAGCAGCAGCTTTGAGCGAGCAAAGTTCTGGG860.10202023796813647No Hit
CGTAGGGGGTATAACTTGGGCAAGTTCCTCAACTTCCCTGTGTATCAGTTTC860.10202023796813647No Hit
TGATTAGGGGAAGGAGTTAAAGGCCTAAATCAAGATATTCTCTTTAGACAAA860.10202023796813647No Hit
TGCCAGGGGCGGCCTGGCGGCCCCAGGACGCAGCAGCAACCTTGGGCTTCCT860.10202023796813647No Hit
TAGTTGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA860.10202023796813647No Hit
TAATCGGGGGTGATAGTTTTCATTAAATTTGGAACAATTTTGACCATCACTT860.10202023796813647No Hit
CAACAGGGAGGGTAACAAATGATGCCCGAGAAAATGAAATGGATGAAAACCT850.10083395613129768No Hit
CTTCGCGGGCCAGGAAAAAGACTGAGGTTCACTAAACTATTTTAAAATGCCT850.10083395613129768No Hit
GTCAAGGGGCCACCACCACGCCCGGCTAATTTTTTGTATTTTTAGTAGAGAC850.10083395613129768No Hit
TTCAACGGGCCAAAGTCATGTATCCTGGCTCTGCTCCAAGCCCTTTAAGGTG850.10083395613129768No Hit
GACAATCGGGTCACTTACCCAGACTGGTATAGAGCTCTGTAATGAAAATGAT850.10083395613129768No Hit
CAGAATGGGGAGAACATGAAGCGCAAACAGAACGACGTCTTTGGAGAAGCTG850.10083395613129768No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)