FastQCFastQC Report
Sat 31 Dec 2022
SRR5846849.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5846849.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences83151
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAAGGAGCTATCTCGTATGCCGTCT7750.9320393019927601RNA PCR Primer, Index 38 (95% over 21bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACAAGGAGCTATCTCGTATGC5060.6085314668494667No Hit
ATACGCGGGTATGGATGAAGAAAAATAATTAGGCATGAAAAATGTAGATAAA1290.1551394451058917No Hit
AGATGTGGGGAATCGTTTAATTTTCTGTAAGGTAATGATGATCATTTGAATG1290.1551394451058917No Hit
CCGATAGGGAAGTCCTGTCACCAAGGACGTTAAAGGCATTTTATTCCAGCGT1280.1539368137484817No Hit
GTTAGGGGGGTTGTTAACTAGATAGCTGTAACTCCAAAAAGACAACTTAAAT1250.15032891967625164No Hit
GGCAATGGGCACTTGCCTTGTTGCTGAAGTTCCAATAATGTGAAAACCAAAG1240.14912628831884162No Hit
GAGTGCGGGTACGGTGGGGTGAACAAGGAGGGCTTGGAATGCCTTCGGCTCC1230.14792365696143162No Hit
GCTGGTGGGGCAAAATAGATAAGTACAACACTCCATTTAGTGTTTTGCCAGA1220.1467210256040216No Hit
CATAAAGGGATTTTAGTCTTTTCTACGATAAAAATTGTATGTGTACTTTCAT1200.1443157628892016No Hit
AGACTGGGGATATGAAGACTTAGAAGAGTAGCATGAGGAAGGAAAAGATAAA1200.1443157628892016No Hit
ACAGCTGGGGGGCCCCGAAGGGCACTGTGAGGGACAGAGGCTCACCTGGGCA1190.14311313153179156No Hit
GGAACAGGGTGGGTGGCTGAGGGGATCGTTAATTGAATGGGGCAGACTGAGG1170.14070786881697153No Hit
GCCTAAGGGATAATCCACCATGATCAAGTGGGTTTCATACCAGGGATGCAGG1150.1383026061021515No Hit
TAACTTGGGATGTTGTGCACTAAAGCAATTGTTCTCTTATCGTAGAATTTTG1140.13709997474474148No Hit
ACTTTAGGGCTAATTGCCCACAAGAGAAAGCAGGAAAGATCTAAAATCAACA1130.13589734338733148No Hit
TGATGCGGGACAAGGGATGAGTACTTTTTAGAGTATGTGCATGTTACGTAAT1130.13589734338733148No Hit
GTCAAAGGGAACTGAGTGTGGTGGCTTATGTCTGTAATCTCAGCCTCCCCAA1110.13349208067251145No Hit
AAGCCAGGGAGGCGAGAAGCCGTTCAAGTGCTACAAGTGCGGCCGTGGCTTC1100.13228944931510145No Hit
GCTTCAGGGACAGTTTAATTTACTACTTCTAAGACAATGCTGAGACCCTATA1100.13228944931510145No Hit
GTCTTGGGCTCTTCCGATCTGCCAGGGGCAGTACAGCCTTTCAGTAAAGAAG1100.13228944931510145No Hit
TGCTCTGGGCTCTGGGAGGGGTGCTGCCTTGTAGATGGAGCAAGTGAGCACT1090.13108681795769142No Hit
AGTTCCGGGGCAGAGGAAAACAGCATGTGGGTATTTGTCGACCAAAATGATG1090.13108681795769142No Hit
CATCAAGGGTGTGCCTTCCTGTGCTCCGGCCACAGGAGCGTCGGCCCAGGGG1080.12988418660028142No Hit
TAGAACGGGTGGACCCAGTATGTCACTTTAATGGACTTCAAGAAAAAATCTG1060.1274789238854614No Hit
ACGAACGGGAGTGCATCTGAAGCTTTTCATATAACGATGATGAAGACGGCTG1060.1274789238854614No Hit
TTAGATGGGATTTGTATCCTTTCTTGTTCTTGTGATCTTGTGATGAAGACTT1060.1274789238854614No Hit
GAGAAAGGACATCCACACCAAAACCCCATCTGTACGTCACCATCATCAAAGA1050.1262762925280514No Hit
ACTTGAGGGAGTCTACTGCGTATTTCCCTGCTCTCTACAGATACCTTATCGC1020.12266839845582132No Hit
GTGCTTGGGCCGTCATCAGGGACTACCTGGTCCAAAAACTACAGAAACAGAG1020.12266839845582132No Hit
GATCGCGGGAGCCTTGTGTGTTATATTACTCGATTTACAGAAGAGGCTTTTG1020.12266839845582132No Hit
TTCCTTGGGAATAATGGAAGTACATACTTGGAAGATGGCTGGGTTAAGAAAT1010.12146576709841132No Hit
AAACATGGGGCAAAAGGAAGTTTTCATCTTTGAACATTAAAGGTATCCTTTA1000.12026313574100131No Hit
AGTTTCGGGTATGTGATTGTTCTTAAAGTCAATGTATTAAATAAGGTATTTT1000.12026313574100131No Hit
GCTGATGGGGGGATGTCAAAGTTGTCCAAAAATGGTTTTATTGGGCAGTGAA1000.12026313574100131No Hit
GTAGAAGGGCAGGGATTATAATTAGTTTACATACATACTCCTTAAACAGATA990.1190605043835913No Hit
GTTGTTGGGATACAGGGAAGAGTCATGATAGGAAACCAAAGGTATCCTGGTA990.1190605043835913No Hit
GAGTGAGGGGCCAGCATTCTGGAGTCACAATAATTAAATAAAATGTAAATCC990.1190605043835913No Hit
AGCGCTGGGATGGCTCCCTCTGTTGAGATTGGAATTGGAAGAGAACTTAATT980.11785787302618128No Hit
CTGCCTATTGAGACAACCAGGTGCATAATTGATTGCCCTTTGGCCATAAAAA970.11665524166877127No Hit
AAGCGTGGGCTTCTAAAGTGCTGGGATTACAGGCATGAGCCACTGCACCCCG970.11665524166877127No Hit
AGTCATGGGAGCAGGCTTTGGAAGTGATAAAGCAGTTAAAAGAGAAAATGAA970.11665524166877127No Hit
CTCGTGGGGGTATTGTATTTCATGGGAGGTATATTTTATGAGCTTTTTGGCT970.11665524166877127No Hit
GGCTTGGGGAACGCAAGTTCCCAGTTGGAGCTCCAGCCTGACATCAAAAAAG960.11545261031136125No Hit
ATATATGGGCAAACGCAGAAGGACTGCCTCACTGAGCAACCAAGAGTGTCAG960.11545261031136125No Hit
GTTAGGGGGTGACTTTTCCAAAAAGGAAACATGAAAACACTCTCATATTCTT950.11424997895395125No Hit
TGAAGCGGGCTGGCATCAGCCTCATACCTAAAACTCCTGACTCTTACCACTC950.11424997895395125No Hit
GTCAAAGGGAATTGGAGATCTCTTTAATTTCGATCAACTTATAATGTGTAGT950.11424997895395125No Hit
CAGTATGGGAACAAATTGCCACTTCCACCAAGGCTAAGGAAAAGCAAGATAA950.11424997895395125No Hit
GTGCCTGGGTGGTGATAATGATGGTGATGGTGATAATGATGGCGATGATAAT950.11424997895395125No Hit
GGTTTTGGGAGAGATTGTCTTTCCTGTTGGAAAAGGCAATCACTAGGGAAGC950.11424997895395125No Hit
TTGTCTGGGACAAGGCATATGAGTTTGTATTTATTTCCTGGAACATGAGAGC940.11304734759654124No Hit
TTGTCGGGGGCAAAGAGGCAGTTCTTCTGGGTCTTGACAGCAGATGTTGGAC940.11304734759654124No Hit
GTCAAAGGGTTAGACAGATCAACAAGACAGAAGATTAACAAGGATATCCAGA940.11304734759654124No Hit
ATCGCTGGGATAAATAGCTAATGGGGGTGGTAGGCCTATAAAATTAAATGCC930.11184471623913123No Hit
CACATGGGGGGAGCTACCTGTGGGGACCTGAGCCATCACTATTCCTGCTTCA930.11184471623913123No Hit
CTCTACGGGAAGCCAGGGCTGGAGAACAAGAAAGGGCCAGGAGATGGAATTC930.11184471623913123No Hit
CAGAATGGGGAGAACATGAAGCGCAAACAGAACGACGTCTTTGGAGAAGCTG930.11184471623913123No Hit
TCTGCCGGGGAAAGTCACAAGGAATCTACCAGATAATCCTAGGACTTGTGAG930.11184471623913123No Hit
CGGCGTGGGTGTCTGGGACACTGACCTGTCAAAACTGGCCCCTGGCTCACTG920.11064208488172121No Hit
AGTAAAGGGACCAGCCTTCCTGCTCTTTCTCATGCTTCATATCTCTCTCCCG920.11064208488172121No Hit
TAGCATGGGCTGGTTATGAGTCACTGTTTTTTTCTCAATGTTAAAATGAGTG920.11064208488172121No Hit
CAAACCGGGAAGGAGACGAGAGGCCCACCATCCCGGACCCAAGTCTTCTTCC920.11064208488172121No Hit
GTTTATGGGACTCTCACCCACGGAAAGCTTCTTTTCATTAGTAGTAATGGAA920.11064208488172121No Hit
CGCAAAGGGGACCTCACAGACAGCAGCAGCTTTGAGCGAGCAAAGTTCTGGG920.11064208488172121No Hit
CGTTAAGGGCCCCACTTGCTCATCAAAGTAGTTCAACCAACATGCTTCTTTC920.11064208488172121No Hit
ATAACAGGGATATGTCAATTTAAAAAAATAAAAGTCTGCTTTGTAAATAGTA920.11064208488172121No Hit
GGTACCGGGGTTGTTTATAGTGGCATCTCATGTTTGAACCCGGGTGGTATCC910.10943945352431118No Hit
TCATAAAGGGAAATTATGGGAATCACTGAAATATTTTTGTAGATTAATTGTT910.10943945352431118No Hit
CTTCGCGGGCCAGGAAAAAGACTGAGGTTCACTAAACTATTTTAAAATGCCT910.10943945352431118No Hit
CGTTGAGGGATATTAAAATTAAAAGGCACTTTTTGAATATAGTGCAAAAATA910.10943945352431118No Hit
CAAGCTGGGGAGGCAAGGAAAGACCAGCAAAGAGACAGAGAAGAAAGAGACA910.10943945352431118No Hit
CGTTCTGGGATCAACTCATTTCACCTTTACTATGATCTGTACCATTCATATT910.10943945352431118No Hit
GGTGTTGGGACTTTGGGAGGCTGAGGTGGGTAGATTACTTGAGCCCAGGAAT910.10943945352431118No Hit
TTAGCAGGGCATTTCTCCTGTTACAATTAAAGTCTTTTTTTCCTATTTTCAG910.10943945352431118No Hit
TCAACAGGGTTATCAACTTAAGGAGATTTTGGGCTGAGACGATGGGGTTTTC910.10943945352431118No Hit
GTGCAAGGGTGAAGGCTGAATCAAAGACATTTCATCCACCAATATCATGTGT910.10943945352431118No Hit
CTGCGCTTGGGACTAGGTAATAAAACCTTCAAAGGTTGTCAAAGGTCATCAA910.10943945352431118No Hit
TGGCCGAGGGATCTGAATCTGTCTCCCAGAATAAAATACTACTTCAAAGGAT910.10943945352431118No Hit
TTCGCAGGGCTTGAACTCCTGGGCTCAAGCAATCTGTCTGCCTCAGCCTCCC900.10823682216690117No Hit
CTATTGGGGTAATGATCACACAGGCAAGGGCCACACATATCACTGGCATCCT900.10823682216690117No Hit
ATGTGAGGGGGCAGACTAGCTGGGTGGCTAGACCCAGAAGAGAGACAATAAT900.10823682216690117No Hit
TGTGATGGGCGGGAGCCACGGCTTCTTGTGGTTACTGACCCCAGGGCTGACC900.10823682216690117No Hit
TAAAGGGGGATCTAAAACGCGGGCGGTGCAGGAGCTGAAGGTGAGTGGAGTG900.10823682216690117No Hit
GAGACCGGGATTCCTTTTCATAGTATGTGCTCCTGCAGAAATGTGACAATGA900.10823682216690117No Hit
GGCGCTGGGATCTCTTAACTTTTATCTTTATTATTTTACAATGCCCTTGTAA890.10703419080949116No Hit
CAACAGGGAGGGTAACAAATGATGCCCGAGAAAATGAAATGGATGAAAACCT890.10703419080949116No Hit
CATAATGGGGCACAGAGGAGTGGATTTAGTAATTGTTAACCTTGTGTCGTAT890.10703419080949116No Hit
CACGTCTGGGAGCTATTATTAGTGTCAATGTAGTATTTAGTATACAGTTAAT890.10703419080949116No Hit
AAGCCAGGGCTTTTGATTGATAAATAATATTTCAGCCTTATTTTTGTAAGGA890.10703419080949116No Hit
CGGCCTCCCAAAGTGCTGGGATTACAGGCATGAGCCTCCGCACCCGAATAAA890.10703419080949116No Hit
GACAATCGGGTCACTTACCCAGACTGGTATAGAGCTCTGTAATGAAAATGAT890.10703419080949116No Hit
AAGCTAGGGATGAACTTTGGTTCTTCATGAGTGCATTTTGAAAAAGAAAAAA880.10583155945208114No Hit
ATATGGGTGAGTATTTCCAGGACAACATTCTAAAAGTACAATTATTTCTTAT880.10583155945208114No Hit
CTTTCCGGGAACTGATGAATAATTGAAATGATTTTTATATTTTTTTGTTTAA880.10583155945208114No Hit
TACAAAGGGGGTTGCCTGCTGCAGTGTCAGTTTCTACTACCTAGCAACCAGC880.10583155945208114No Hit
CCGCCGGGAGGCTGATAAGCAATTTCAGCAAAGTCTTAGGATACAAAATCAA880.10583155945208114No Hit
TCTAAGGGGATTCTTCAGGTAGATAATTTTTCAAATGTGAATTATCTTTTGT870.10462892809467114No Hit
ACTGGAAAGGGCATTGGAGAAAACAGGTGTGTATAAGAGTACAGGAAAACAG870.10462892809467114No Hit
CTCAGGGTAATCATCTTGCTCCTTGCTTAAAGCATATCTTATTATATATAAT870.10462892809467114No Hit
GTCGCCGGGATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTA870.10462892809467114No Hit
CGTCCTGGGTCACCATCAAGATTAGATATCTTTGATTAGTTTTGGTAACTTC870.10462892809467114No Hit
GTCTAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA870.10462892809467114No Hit
CCCTGTGGGCTTTGTTCACGGCTCTCCTTAATGACTGAGTGAACAGTTCCTA870.10462892809467114No Hit
TTATATGGGATGTAAGTTGTGCCGTCATTTGTTTTGCTTAACAGTTTTTTTG870.10462892809467114No Hit
TTAGGTGGGCACCTTCTTATTTGACATACAAACATTGTGGATTTTATCTTGC870.10462892809467114No Hit
ATGTGGGGGTTTGCAGAAACATCTAAGAAGCGCTGAATTCTAGTTCAAGCCC870.10462892809467114No Hit
CCTCGAGGGCAAGTTCCCTGAGGCACCTACTGTCAAGGCCTCCTGTGGCTTC860.10342629673726113No Hit
TGCAAAGGGATTTTGAAAAAGAAAAAAAGTACACATTCAGAATCTGGCCAAT860.10342629673726113No Hit
GCGCTGGGTGAGGATGGTCTCATTCATAGACCTCTCAGACACGGTGGTAATT860.10342629673726113No Hit
TAATCTGGGTAAGGAATAGCAGGAGAAATTTTACTGAAGTGCTGTTTTCCTA860.10342629673726113No Hit
GAGCAGGGAAGCTGAGTCCAGCACAGACCTCGCCCTGAATCTCCATGCCCAA850.10222366537985111No Hit
TTCGCCGGGCTGAGCGATGCAAGACACAGCAAAGCCACCTGAAGCAACCACA850.10222366537985111No Hit
GTTGTAGGGTGAGCAGCCTCTTGTAAAATAGCTAGAACAAGGGCAAGGATAT850.10222366537985111No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGGAGCT850.10222366537985111No Hit
CCGGCTGGGATTGACATTTTGACAGTGATTCTAGGCTTTGTGGTGTTGTTCA850.10222366537985111No Hit
GCTAGAGGGGGAAAATAGGACACATTATTAGCCCAGTTCTAAATCCAGTTCC850.10222366537985111No Hit
GCATAGGGGGCCTTTCGGCCGGAACCGCCATCTTCCAGTAATTCGCCAAAAT850.10222366537985111No Hit
CCAAGTGGGAGTGTCAATGTTTGTCAAAATGTGACTCTTTGAATATTCTAAT850.10222366537985111No Hit
ACCGCTGGGACCAGTGCAACAGCATCTTCAAAGAAAACAATTGAAGATAGAA850.10222366537985111No Hit
GTTCCAGGGTAAGCAAATTTCATTTCACATTTTTGTAATGCTGTACAGCAGT850.10222366537985111No Hit
CCGTCCAGGGCCCCACATCGATGAAAGCAAAGCGAAGCCACCAAGCCATCAT850.10222366537985111No Hit
GACTAAGGGGACAGAAAAGACATCTAAGAAAGAAAACTAGCAAATTTATCTT840.1010210340224411No Hit
TTTCAGGGGTCCGGAGCGCGCACACAGATCCGGAGCGCGCACACAGATCCGG840.1010210340224411No Hit
TGCGCTGGGTACACGACGCACTTCCCACATATAGATACGTGTTTAGTGACAG840.1010210340224411No Hit
AGTGCTGGGTGGAAGCTATTGGAACTTTTGTATTCAGTGCTAAATACTAAGT840.1010210340224411No Hit
TTATTTGGGATGTGCCTGCTGATGACTTGACTGACCCTGCCCCTGCTACTAC840.1010210340224411No Hit
TTGGCCGGGGTTGAAGGGACTTTGTTGGCTTTTAGAGTGTTGGTCATGACTC840.1010210340224411No Hit
CAAATGGGGTTAATGTAAACCTCCTGAGATTGGAAGACAATCAGTTTTACAG840.1010210340224411No Hit
AGACACGGGTACACACCATCAGCTTGTAAGTGGTCACAATTCAATTTTGCAA840.1010210340224411No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)