FastQCFastQC Report
Sat 31 Dec 2022
SRR5846839.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5846839.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences188634
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAAAGGTCCATCTCGTATGCCGTCT15920.8439623821792466RNA PCR Primer, Index 16 (95% over 21bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACAAAGGTCCATCTCGTATGC13560.7188523808009161No Hit
CTTATACACATCTCCGAGCCCACGAGACAAAGGTCCATCCTGTCTCTTATAC2550.1351824167435351No Hit
GGTACCGGGCAAGAAGAAAGTGAGGAAGTTGGAAGATCAGAACGAGTATGAA2430.12882089124972168No Hit
CCGCGAGGGCGAACGAAAAATTCTAGGCTATATACAACTACGCAAAGGCCCC2410.12776063700075277No Hit
ACCGTCGGGGTCCTTTTGAATTAAGAGATTTAGTTATGTTTACTAAGAATGT2360.12511000137833053No Hit
ACTTACGGGAGACGTAGACACACGAGCATATTTCACCTCCGCTACCATAATC2350.12457987425384606No Hit
GCTTCGGGGTATTTTGTGAAGATCTTAAATTTGTGGTATAAGAACTTGAAGG2340.12404974712936162No Hit
CAATATGGGGATAACTAAAAATTGGACATAAGAGAGACAGTGTCCAGGAAAG2340.12404974712936162No Hit
GTTATCGGGCTCGGCAGTGGTACCTGTTCCCGGTGTCCCTGAGGACGTGCGG2320.12298949288039272No Hit
CGTGGCGGGTAACATAAAGCAAGTGAGCGACCTTATCTCTGTGCTTCGTGAA2320.12298949288039272No Hit
TTAGTAGGGGATCTCATGACGGGAGCAGAGGATTCCTCTGGGCCGGTGATTT2310.12245936575590827No Hit
GACTTGGGGAAGACATGGAAGTTGGGAGGCACTGAATCAATGAAACTGCCAA2310.12245936575590827No Hit
CAGCAAGGGCATCACCTCGGTCGTCCCTTCCCACCAGGCTACTGTGCTACTC2300.12192923863142381No Hit
GGATGTGGGTGCTTCTGGCCCCAAAGTTGTAAAACACATATGTATGGCATAT2300.12192923863142381No Hit
TCTACAGGGGCTTTTCCTCCGCTGGAGCAGCTATGGCGGCGGTGAAGACCCT2290.12139911150693937No Hit
CCGCACGGGGAACCTTTTCAGTAGGGAAATTCAGAAAATGGAATGATTCATC2260.119808730133486No Hit
GCGACGGGGCTGCTGCTTTCCTTCGGAATCCAGTGCTTCCACAGAGATTAGC2260.119808730133486No Hit
GTTTATGGGAAGATTGAGCCTCCGCTGATTTTCGAGTGTAACCAGGCGTGCT2260.119808730133486No Hit
TGGCATGGGAATTGGGCATGGTGGTGGACACCTGTAATCCCAAAATGTTCAG2240.1187484758845171No Hit
GACAATCTTGGCTCTGATGAAGACCCCAGTCCTCTTTGACATTTATGAGATA2230.11821834876003265No Hit
ATGCAGGGGTGCTCAGAATATCATATAATGTTTATTTTTTGAGGTGTTTTTG2230.11821834876003265No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACAAAGGTCCATCCTGTCTCT2210.11715809451106375No Hit
ACAGACGGGCTTGCTAACAAATTCTTCCTAATAGAGGTCCTGTTAGAGTCAA2200.1166279673865793No Hit
ACTTAGGGGCTGAAAAGGGAGGAGTATTTTTAGTTTGACAATTTAATAATTT2200.1166279673865793No Hit
GCATGTGGGAGTCCGAGTGGAGAGAGCGAGCTGAGTGGTTGTGTGGTCGCGT2190.11609784026209485No Hit
GTCTGCGGGACTATGTCTCAAGGAGAGATTACCAAGTTGGCAGTGAGACAGA2190.11609784026209485No Hit
CTCATTGGGGGCCACGTGATCTTATTTTAAAAGAACACTGCTATAGAAGAAT2170.11503758601312596No Hit
TGCTGCGGGTTAAAGATAAATTTAAGAAGACTCAAAATAGAAAGGATACAGA2150.11397733176415706No Hit
ATTCTGGGGAATTAAAGTTGGATAGAGAAACAGACACATGTGATGAAGCAAA2150.11397733176415706No Hit
ACTCAGGGGCCCTGAACTTGGCTCAGCTGCCGGGCTGCTCCGGTTGGAAACG2140.11344720463967259No Hit
GCTGCAGGGCAGCAAAAACAGGAGAATCACATGCTTTAAAGAACACTTTCTC2140.11344720463967259No Hit
GTGTGCAGGGAGGCGCCCGGTGGCTCCGAAGCGGGGAAGTGGGACAAGATGG2120.11238695039070369No Hit
AAAATCTACTGCTCTTTCTGAATCTCCAGATATCATTTCTGTATGGTTTCTG2120.11238695039070369No Hit
TCGGGCGGGTAAGAAACACAATTAATGTATATGAAACAGCGACATCCACAGA2110.11185682326621924No Hit
AGTAAGGGAAATCATAAACCTTGGCTTTCATGGTTTACATTATTTTGTAAAT2100.1113266961417348No Hit
CAGACTGGGAGGTTTATCTTATATCAGTAAATCAGTTTCTAGCAACAAAGCT2100.1113266961417348No Hit
TTGTCTGGGAGATGAAGACATTAATAAAAGGTAAAATTATTTTTACATTTGA2100.1113266961417348No Hit
GCCCATGGGCTTGTGATGGGACAGAAATAAACCTCTTACAGAGTCAACAGGA2080.11026644189276588No Hit
GTCGACGGGCAGCTGAGAGTGAAGCAAAAGTAAAAACCAAAGTTCGCTTTGA2070.10973631476828144No Hit
GCAGTGGGAACATGTTGCTTTGTATAAAAATCTTATTTATAAATGTGAAGCT2070.10973631476828144No Hit
CTTCAAGGGATTGGCAACAAATACTGTTATTTTCCTTAACATGACAGGCTCA2070.10973631476828144No Hit
TCAGCCGGGTTATACATGCAAAATAGAAAAAAAATGTTCAACATTTATTGAT2040.10814593339482809No Hit
ATCCTTGGGATCAGGATTTAGTGACCTAATTGAAGATGTCAAGATGGCTTTC2020.10708567914585919No Hit
ATTGTCGGGGACGCGCACGAACGCACGGCCGCGCAGCATCTGTCTTGCTGGA2020.10708567914585919No Hit
ACTGTAGGGACGTATCCTATATTTTGAATTTTATACTAGTTTTTCAAAAGTA2020.10708567914585919No Hit
CCAACGGGATGAGGGAATTCCGGTCAGGGTCAAGTCGTGTTCTGATCACTAC2010.10655555202137472No Hit
CGTGCCGGGGTCCGGACAACCAAAAATGAAGAGGAGAATGAAAAGGAAAAGG2010.10655555202137472No Hit
ACTTGTGGGTTGCTAGATAACCACATATTCCACTTAGAGATTAGCATACATC2010.10655555202137472No Hit
CGCTGGGGGGGCCCCAGTCACTGAGCCGCCGCCGAGGATTCAGCAGCCTCCC2010.10655555202137472No Hit
GCCGCGGGGAAGAGTTTTCAGTAGAATATTTAAGAACTAATTTTCTGCAGCT2000.10602542489689029No Hit
TGTGGAGGGACTTTCAAATTATTTGAATTACCAAGACGTCTGCTTTTGTGAC2000.10602542489689029No Hit
TGCGCTGGGAGGGACCAATCCTGGAGAGATAGAGATTTGCGGCCTTTCAGAC2000.10602542489689029No Hit
TACAACTGGGAATGTAAAGGATGAGACAAATTAGAGAAATATTGTGAAGGCA1990.10549529777240582No Hit
TGATGCAGGGGCGCTTAAAAGGCGGTGGCGGTGGCGGCAGCGCCCGGCGCCC1990.10549529777240582No Hit
ATTGTTGGGACCAATCAGAATTCGAAGGATTTTCCTTTGTTAACTCTGAATT1990.10549529777240582No Hit
GATCACGGGTCTTGGGAATTTTGTATACATTATGGAATATTAGGATAAGTTT1990.10549529777240582No Hit
GTAAAGGGGATGACAGAACAGTGATCACATATATTTGGATTTATATGATATA1990.10549529777240582No Hit
GCTTTCGGGATCCCTGTTTCACTCCCAAACCCTCTCAAATTCATTTCTTCAC1970.10443504352343692No Hit
CAGCATGGGGAAGGAGCTCAAGGAGCAGCTGGAACAAGCCAAGAGCCACGGG1960.10390491639895247No Hit
AGTGCCGGGAAGGAACTCCGGTGCAAAGCGTGATGGTTTCCTACCCAACAAT1960.10390491639895247No Hit
ACACGTGGGATAACAGAATTCCTCTCTTCTCCAACTCGTTTTATATATGCTG1950.10337478927446803No Hit
CCATTAGGACTGGTTATTGTAATAAACCACCCATGGTTCTTTATGTACATTT1950.10337478927446803No Hit
GGGTACGGGAACATACATTGCTTGGCCAAAGCCATCAACCAGATTGCTGCAG1940.10284466214998357No Hit
ACAGAAGGGTGTTGAGGACTGGGACTTAGTTACCAGGCAGAAGCAGCTGTTT1930.1023145350254991No Hit
AAAATAGGGATATTTGAAAAGGCTTCCATTAATATAATAGCTTTGCTTGCAG1930.1023145350254991No Hit
CGCTATGGGACATATTTCAAAGTTAGATATGTGTCTTTGAATATATTTAGAA1930.1023145350254991No Hit
ATCAACGGGGAAGTTATGAGGGATACAAGTGAAATACTGGGGAAGAAAATGG1930.1023145350254991No Hit
TCTAAAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1920.10178440790101467No Hit
CTTATAGGGCATTTGAAATAAGTTATGGCTTAAAATGCTTTTGGAGTTTATT1920.10178440790101467No Hit
AGCTTGGGTGAGCTAGCATAGTCTTTCAAATGTAAATATTTCAATATTTGCT1920.10178440790101467No Hit
GTGCAGGGGAGAAGGACTTGGAGGACCTGTTCTACAAGTACGGCCGCATCCG1900.10072415365204578No Hit
ACATTAGGGCAGCGTGGGGGCGGCAGGAGGGAAGATGGCGGACGAGGAGAAG1900.10072415365204578No Hit
TCTTTAGGGTTCATATGTTTCCTCAACTGTACAATGAACTATTCTCATGAAA1900.10072415365204578No Hit
TGCTCTCGGGACCACCTTCAACTCCATCATGAAGTGTGACGTGGACATCCGC1890.1001940265275613No Hit
CTGGATGGATTAAAGAAACCACAGGGATTTCTGCTCTTGTGTAGTTGTGTAT1890.1001940265275613No Hit
CCTGGTGGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCC1890.1001940265275613No Hit
CTATTTGGGACAGCTGTGAAGAGAAAGTTTCCCAGTGAGTTAATAAGTTGGC1890.1001940265275613No Hit
CTCTCAGGGTACCTGGTACTGTGGCACCTCCCAGAAAGTCTGGTTTTTGCCC1890.1001940265275613No Hit
AGCAGGGGGCAGCGAATCAAGCGGGAGTTCAAGGAGGTGCTGAAGAGCGAGG1890.1001940265275613No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)